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Current Zoology 2014年第4期60卷 460-471页

作者： Rupesh k. LUHARIYA kuldeep k. lal Rajeev k. SINGH Vindhya MOHINDRA Arti GUPTA Prachi MASIH Arvind k. DWIVEDI Rak.i DAS U. k. CHAUHAN J. k. JENA National Bureau ofFish Genetic Resources (ICAR) Lucknow- 226 002 UP India School of Environmental Sciences APS University Rewa- 486 003 MP India

ATPase 6/8 gene (842 bp) of mitochondrial DNA was sequenced inLabeo rohitasamples (n= 253) collected from nine rivers belonging to four river basins; Indus, Ganges, Brahmaputra and Mahanadi. Analysis revealed 44 haplotypes with high haplotype diversity (Hd) 0.694 and low nucleotide diversity (π) 0.001. The within population variation was larger (83.44%) than among population differences (16.56%). The mean FSTvalue (0.166;Pk.presented a single clade for wildL. rohitapopulation, from different rivers. Negative values for Fu’s index (FS), mismatch distribution analysis indicated period of expansion inL. rohitapopulation. The time after recent expansion was estimated for each population, between 0.042 to 0.167 mya. The pattern of Isolation by Distance (IBD) was not significant (r= -0.113,Pk.) was seen. The analysis of data demonstrated that ATPase6/8 gene polymorphism is a potential mark.r to understand genetic population structure of wildL. rohitaexisting in different rivers. The study identified population substructure in wildL. rohitawith common ancestral origin [Current Zoology60 (4): 460–471, 2014].

关键词：线粒体DNA 系统地理学基因分析 ATP酶野鲮鲤科鱼类南亚家谱

Identification of polymorphic allozyme mark.rs for assessing genetic variability in Labeo dyocheilus (McClelland,1839)

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动物学报 2005年第1期51卷 167-170页

作者： Peyush PUNIA lalit NARAIN Vindhya MOHINDRA Anshumala BHARDWAJ Rajeev k.mar SINGH Dhurendra k.POOR kuldeep k.mar lal National Bureau of Fish Genetic Resources(ICAR) Canal Ring RoadP.O.DilkhushaTelibaghLucknow-226002(UP)India

The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food *** is native to Afganistan,Pak.stan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambodia and Thailand(Froese and Pauly,2003).In India,the species in-habits the Indus,Ganges and Mahanadi river *** is identified as a potential cultivable species and has considerable significance for the fishery of these *** has been categorized as a vulnerable fish due to declining commercial catches in India(Mahanta et a1.,1994).At present the fishery is supported through capture from natural *** achieve domestication,various aspects of captive breeding and propagation are being studied.

关键词： Labeo dyocheilus Polymorphic allozyme Genetic variability

使用RAPD分析辨别印度半岛的五种结鱼(英文)

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动物学报 2007年第4期53卷 725-732页

作者： Vindhya MOHINDRA Praveen k.ARE kuldeep k.lal Peyush PUNIA Rajeev k.SINGH Anindya Sundar BARMAN W.S.LAk.A National Bureau of Fish Genetic Resources(ICAR) Canal Ring RoadP.O.DilkhushaLucknow-226002 (UP)India

用RAPD分析研究了结鱼种组(黄鳍结鱼Tor putitora,结鱼Tor tor,库德里结鱼Tor k.udree,Tor mosalmahanadicus和墨脱四■魮Neolissochilus hexagonolepis)5个物种的遗传关系。在所测试的69个随机引物中,11个引物能够在所有5个物种中扩增出稳定的条带。RAPD带型显示,综合使用这些RAPD标记能够区分这5个物种,但Tor mosal mahanadicus和黄鳍结鱼享有相似的带型。UPGMA分析揭示出3个独特的分支,第一支由黄鳍结鱼、Tor mosal mahanadicus和结鱼组成,第二支是库德里结鱼,第三支是墨脱四■魮。Tor mosal mahanadicus的分类地位在不同学者间存在分歧,被认为是库德里结鱼或结鱼的亚种,但我们的结果表明,相对而言,Mahanadi河中的Tor mosal mahanadicus与黄鳍结鱼的进化关系更近,因此有必要对其系统分类地位进行重新评估。

关键词：结鱼印度 RAPD标记物分子辨别聚类

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鲤科鱼蓝黑鲮微卫星位点的种间扩增(英文)

动物学报 2008年第5期54卷 937-940页

作者： Rajeev k.mar SINGH kuldeep k.mar lal Vindhya MOHINDRA Peyush PUNIA Wazir Singh LAk.A National Bureau of Fish Genetic Resources(ICAR) Canal Ring RoadP.O.DilkhushaLucknow 226002(UP)India

本研究使用105对微卫星引物对7种鲤科鱼类进行跨越种间PCR扩增,共得到14个多态性微卫星位点。其中9个扩增效果较好的位点用于分析来自帕吉勒提河(Bhagirathi,n=20)和戈达瓦里河(Godavari,n=25)的蓝黑鲮(Labeocalbasu)样品的遗传多样性... 详细信息

本研究使用105对微卫星引物对7种鲤科鱼类进行跨越种间PCR扩增,共得到14个多态性微卫星位点。其中9个扩增效果较好的位点用于分析来自帕吉勒提河(Bhagirathi,n=20)和戈达瓦里河(Godavari,n=25)的蓝黑鲮(Labeocalbasu)样品的遗传多样性。结果显示,前者在每个位点的平均等位基因数为7.33,而后者为8.11,期望杂合度介于0.795(Bhagirathi)和0.801(Godavari)之间;4个位点MFW11*(Godavari)、R1*(Godavari)、R3*(Bhagirathi)和Lr38*(Bhagirathi和Godavari)都表现出明显的杂合子缺失和哈迪温伯格平衡偏离;而任意两位点间都未观测到连锁不平衡现象;位点R3*极可能存在无效等位基因。上述结果表明这些多态性微卫星位点作为共显性标记在蓝黑鲮群体遗传学研究中有着较好的应用前景。

关键词：蓝黑鲮跨越种间扩增微卫星位点多态性遗传变异无效等位基因

印度恒河蓝黑鲮与南亚黑鲮杂交个体的分子检测(英文)

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动物学报 2007年第4期53卷 773-776页

作者： kuldeep k.lal Rajeev k.SINGH Vindhya MOHINDRA Anup MANDAL Peyush PUNIA B.N.MISHRA W.S.LAk.A National Bureau of Fish Genetic Resources(ICAR) Canal Ring RoadP.O.DilkushaLucknow-226002UPIndia Department of Biotechnology Institute of Engineering & TechnologyU.P.Technical UniversityLucknowUPIndia

利用鉴别性的同工酶位点从印度恒河水系的支流Banganga中检测到了蓝黑鲮(Labeo calbasu)与南亚黑鲮(Labeorohita)的一个杂交个体。线粒体DNA的单倍型模式进一步确认这个杂交个体的母本是河蓝黑鲮。

关键词：蓝黑鲮南亚黑鲮杂交同工酶 mtDNA

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用微卫星标记评估红尾鲃的种群结构(英文)

动物学报 2004年第4期50卷 686-690页

作者： Achamveettil GOPALAk.ISHNAN k.chik.aran k.njumohammed MUSAMMILU Peringady Mohammed ABDUL MUNEER kuldeep k.mar lal Dhurendra k.POOR Alphis Geethanand PONNIAH Vindhya MOHINDRA National Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) IndiaNational Bureau of Fish Genetic Resources (NBFGR ICAR) Canal Ring Road P O. Dilkhusha Telibagh Lucknow 226002 (UP) India

本文检测了三种鲤科鱼的 1 6对微卫星引物在红尾中的适用性 ,其中 6对引物可以成功扩增 ,且 5个位点具有多态性。对采自两条不同河流的标本 ,通过检测这些多态微卫星位点的遗传变异情况 ,评估了它们在红尾种群结构分析的适合性。结... 详细信息

本文检测了三种鲤科鱼的 1 6对微卫星引物在红尾中的适用性 ,其中 6对引物可以成功扩增 ,且 5个位点具有多态性。对采自两条不同河流的标本 ,通过检测这些多态微卫星位点的遗传变异情况 ,评估了它们在红尾种群结构分析的适合性。结果显示这 5个多态位点在上述两个样本中的平均表观杂合度分别是 0 2 93和0 4 71。

关键词：微卫星遗传变异鲤科红尾鲃种群结构