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Neuroscience Bulletin 2017年第3期33卷 292-298页

作者： Dahu Li Lijun Zhang Xin Huang Lili Liu Yunling He Lun Xu Yiyao Zhang Tong Zhao Liying wu Yongqi Zhao kuiwu wu Yan wu Ming Fan Lingling Zhu Department of Cognitive Science Institute of Basic MedicalSciencesBeijing 100850China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Beijing Institute for Brain Disorders Beijing 100069China Navy General Hospital of the PLA Beijing 100048China Air Force General Hospital of the PLA Beijing 100142China

The hypobaric hypoxic environment in highaltitude areas often aggravates the severity of inflammation and induces brain injury as a consequence. However, the critical genes regulating this process remain largely unknown. The phosphatase wild-type p53-induced phosphatase 1（WIP1） plays important roles in various physiological and pathological processes, including the regulation of inflammation in normoxia, but its functions in hypoxic inflammation-induced brain injury remain unclear.Here, we established a mouse model of this type of injury and found that WIP1 deficiency augmented the release of inflammatory cytokines in the peripheral circulation and brain tissue, increased the numbers of activated microglia/macrophages in the brain, aggravated cerebral histological lesions, and exacerbated the impairment of motor and cognitive abilities. Collectively, these results provide the first in vivo evidence that WIP1 is a critical neuroprotector against hypoxic inflammation-induced brain injury.

关键词： Hypobaric hypoxia Inflammation Brain injury WIP1 phosphatase Lipopolysaccharide

Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure

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Science China(Life Sciences) 2023年第12期66卷 2786-2804页

作者： Shuting Yu ZhiYong Sun Xiuzhu Wang Tiantian Ju Changhao Wang Yingqi Liu Zhezhe Qu kuiwu Liu Zhongting Mei Na Li Meixi Lu Fan wu Min Huang Xiaochen Pang Yingqiong Jia Ying Li Yaozhi Zhang Shunkang Dou Jianhao Jiang Xin Li Baofeng Yang Weijie Du Department of Pharmacology(The State-Province Key Laboratories of Biomedicine Pharmaceutics of China Key Laboratory of Cardiovascular ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbin 150081China Northern Translational Medicine Research and Cooperation Center Heilongjiang Academy of Medical SciencesHarbin Medical UniversityHarbin 150081China Research Unit of Noninfectious Chronic Diseases in Frigid Zone Chinese Academy of Medical Sciences2019RU070Harbin 150081China Traditional Chinese Medicine School Beijing University of Chinese MedicineBeijing 100029China

Ischemic heart failure(HF)remains a leading cause of morbidity and mortality.Maintaining homeostasis of cardiac function and preventing cardiac remodeling deterioration are critical to halting HF progression.Methyltransferase-like protein 13(Mettl13)has been shown to regulate protein translation efficiency by acting as a protein lysine methyltransferase,but its role in cardiac pathology remains unexplored.This study aims to characterize the roles and mechanisms of Mettl13 in cardiac contractile function and HF.We found that Mettl13 was downregulated in the failing hearts of mice post-myocardial infarction(MI)and in a cellular model of oxidative stress.Cardiomyocyte-specific overexpression of Mettl13 mediated by AAV9-Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to MI,while silencing of Mettl13 impaired cardiac function in normal mice.Moreover,Mettl13 overexpression abrogated the reduction in cell shortening,Ca^(2+)transient amplitude and SERCA2a protein levels in the cardiomyocytes of adult mice with MI.Conversely,knockdown of Mettl13 impaired the contractility of cardiomyocytes,and decreased Ca^(2+)transient amplitude and SERCA2a protein expression in vivo and in vitro.Mechanistically,Mettl13 impaired the stability of c-Cbl by inducing lysine methylation of c-Cbl,which in turn inhibited ubiquitination-dependent degradation of SERCA2a.Furthermore,the inhibitory effects of knocking down Mettl13 on SERCA2a protein expression and Ca^(2+)transients were partially rescued by silencing c-Cbl in H_(2)O_(2)-treated cardiomyocytes.In conclusion,our study uncovers a novel mechanism that involves the Mettl13/c-Cbl/SERCA2a axis in regulating cardiac contractile function and remodeling,and identifies Mettl13 as a novel therapeutic target for ischemic HF.

关键词： heart failure Mettl13 SERCA2a c-Cbl ubiquitination

Geophysical Features of the Ore-Controlling Fault in the Chang'an Gold Deposit, Southern Yunnan Province

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Acta Geologica Sinica(English Edition) 2015年第5期89卷 1771-1772页

作者： LI Hua ASKAR ZHOU Yunman ZHANG Wei wu Wenxian ZHOU Yimin ZHOU kuiwu Chengdu Center of China Geological Survey Yunnan Gold & Mining Group Co.Ltd.

The Ailao Mountain is one of the most important metallogenic belts ofpolymetallic deposits in the Sanjiang region, southwestern China. Located in the southern segment of this metallogenic belt, the newly-discovered Chang＇an gold deposit is large in scale （Fig. 1A）, and has attracted much attention among geologists. The ore-hosted rocks in the district include the Late Ordovician Xiangyang Fm. sandstone and clastic rocks and the Early Silurian Kanglang Fm. dolomite. Affected by the multistage tectonic activities, stocks and dykes of lamprophyre, dolerite, syenite porphyry and orthoclasite are widely exposed, and the orebodies are in symbiosis with or crosscut the dyke rocks.

关键词： Gold Southern Yunnan Province Geophysical Features of the Ore-Controlling Fault in the Chang’an Gold Deposit

Loss of cell adhesion molecule CHL1 improves homeostatic adaptation and survival in hypoxic stress

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Loss of cell adhesion molecule CHL1 improves homeostatic ada...

Loss of NB-3 Aggravates Cerebral Ischemia by Impairing Neuron Survival and Neurite Growth

中国神经科学学会第十届全国学术会议

作者： Xin HUANG Jia SUN Weifang RONG Tong ZHAO Li-ying wu kuiwu wu Melitta SCHACHNER Zhi-Cheng XIAO Ling-ling ZHU Ming FAN Institute of Molecular and Clinical Medicine Kunming Medical College

Objective CHLl is a transmembrane cell adhesion molecule that is critical for brain development and in the maintenance of neural circuits in the adult.Recent studies revealed that CHLl has diverse roles and is involved in the regulation of recovery after spinal cord injury.CHLl expression was down-regulated in the cerebral cortex,hypothalamus and brain stem after induction of acute hypoxia.In the current study we sought to address the role of CHLl in regulating homeostasis responses to hypoxia using CHLl knockout(CHLl) mice.Results We found that,compared with the wild type littermates,CHLl mice showed a dramatically lower mortality rate and an augmented ventilatory response after they were subjected to acute hypoxia.Immunofluorescence staining revealed that CHLl was expressed in the carotid body(CB),the key oxygen sensor in rodents and CHLl expression level in CB as assayed by western blot was decreased after hypoxic exposure.The number of glomus cells and the expression of tyrosine hydroxylase(TH,a marker for glomus cells) in CB of CHLl mice appeared to be increased compared with CHL1mice.In addition,in the ex vivo carotid body preparation,hypoxia induced a significantly greater afferent nerve discharge in CHLl mice compared with the CHL1 mice.Furthermore,the arterial blood pressure and plasma catecholamine level of CHLl mice were also significantly higher than those of CHL1 mice.Conclusion Our findings first demonstrate that CHLl is a novel intrinsic factor that is involved in carotid body function and in the ventilatory response to acute hypoxia.

关键词： CHL1 acute hypoxia homeostatic adaptation survival

来源： cnki会议评论

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生理通讯 2014年第3期33卷 70-77页

作者： Xin Huang Jia Sun Tong Zhao kuiwu wu Kazutada Watanabe Zhi-Cheng Xiao Ling-ling Zhu Ming Fan Department of Brain protection and Plasticity Institute of Basic Medical Sciences No.2 7 Taiping Road Beo'ing 100850 China

2013年10月12—13日中国生理学会张锡钧基金会第十二届全国青年优秀生理学学术论文交流会在湖南长沙顺利召开。由各省生理学会推荐的37名参赛选手的论文参加评选，会议展示了选手们近3年来在生理学研究方面所取得的最新研究成果。经过... 详细信息

2013年10月12—13日中国生理学会张锡钧基金会第十二届全国青年优秀生理学学术论文交流会在湖南长沙顺利召开。由各省生理学会推荐的37名参赛选手的论文参加评选，会议展示了选手们近3年来在生理学研究方面所取得的最新研究成果。经过专家对参评者论文和现场报告的综合评判，评出一等奖、二等奖、三等奖、特别奖、最佳表达奖、最佳答辩奖和最佳图表奖共11名。从2013年第6期开始，《生理通讯》将陆续转载获奖者的参评论文各一篇，以飨读者。

关键词：生理学论文研究成果基金

罗汉松内生真菌Pestalotiopsis heterocornis的代谢产物研究

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广西植物 2019年第11期39卷 1568-1572页

作者：鲍真真吴洁王奎武吴斌钟嫄江苏卫生健康职业学院药学院南京211800 浙江工商大学食品科学与生物技术学院杭州310035 浙江大学海洋学院浙江舟山316021

从罗汉松内生真菌Pestalotiopsis heterocornis的发酵培养液中分离得到10个代谢产物,应用质谱、核磁共振等现代波谱学方法鉴定为jesterone(1),hydroxy-jesterone(2),ambuic acid(3),6β-羟基-豆甾-4-烯-3-酮(4),(24 S)-麦角甾-5-烯-3β,7α-二醇(5),7,22-二烯-3β,5α,7β-三羟基-麦角甾醇(6),麦角甾-7,22-二烯-3-酮(7),(4 E,8 E,2 S,3 R,2′R)-N-2′-羟基棕榈酰-9-甲基-4,8-sphingadienin(8),鲨肝醇(9),棕榈酸(10)。以上化合物均为首次从内生真菌P.heterocornis的代谢产物中分离得到,化合物4,6,7为首次从拟盘多毛孢属真菌代谢产物中分离得到。

关键词：内生真菌 Pestalotiopsis heterocornis 代谢产物

解郁祛痰化浊方干预高脂饮食大鼠肠道菌群多样性的探讨

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天津中医药 2022年第3期39卷 375-379页

作者：李娜吴阳阳段锦龙郑旭彤姚魁武北京中医药大学北京100029 洛阳市中医院心血管科二病区洛阳471000 中国中医科学院广安门医院北京100053

[目的]探讨解郁祛痰化浊方干预高脂饮食大鼠肠道菌群的多样性及对血脂、体质量的影响。[方法]将70只雄性SPF级别Wistar大鼠随机分为正常组,模型组,血脂康组,立普妥组,解郁祛痰化浊方低剂量组,中剂量组,高剂量组。每组10只。正常组喂养... 详细信息

[目的]探讨解郁祛痰化浊方干预高脂饮食大鼠肠道菌群的多样性及对血脂、体质量的影响。[方法]将70只雄性SPF级别Wistar大鼠随机分为正常组,模型组,血脂康组,立普妥组,解郁祛痰化浊方低剂量组,中剂量组,高剂量组。每组10只。正常组喂养正常饲料,模型组喂养高脂饲料。连续灌胃给药8周后收集粪便,进行16S rRNA基因测序,测量体质量,腹主动脉取血检测胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平。[结果]与正常组比较,模型组大鼠体质量明显增加(P<0.01)。与模型组比较,血脂康组,解郁祛痰化浊方低、高剂量组大鼠体质量明显下降(P<0.05)。模型组大鼠的血脂4项TC、TG、LDL-C均见明显升高,HDL-C降低(P<0.01)。同模型组比较,各用药组TC、TG均见显著降低,其中解郁祛痰化浊方中剂量组显著降低LDL-C水平(P<0.01),解郁祛痰化浊方高剂量组以及血脂康组可提高HDL-C水平。正常组厚壁菌门较模型组低(P<0.01),拟杆菌门较模型组高(P<0.01)。拟杆菌门解郁祛痰化浊方中剂量组较模型组增高(P<0.05)。厚壁菌门/拟杆菌门(F/B)模型组显著高于正常组(P<0.01),解郁祛痰化浊方中剂量组低于模型组(P<0.01)。[结论]高脂血症以气郁痰阻为病机,解郁祛痰化浊方立方精准,调节高脂饮食大鼠血脂,降低体质量,调节F/B的比值及肠道菌群多样性。

关键词：肠道菌群解郁祛痰化浊方高脂血症 16S rDNA测序

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加速康复外科理念在肝切除术中的应用

中国普通外科杂志 2019年第8期28卷 989-994页

作者：于江涛任魁梧吴盼盼孙杰杨锐宋坤安徽省阜阳市人民医院肝胆胰外科

目的:探讨加速康复外科(ERAS)理念在肝切除术围手术期中的应用效果。方法:选择2014年2月-2018年12月于阜阳市人民医院行肝切除术的97例患者,按随机序贯原则分为ERAS组(49例)和对照组(48例),ERAS组患者采用ERAS理念行围手术期管理,对照... 详细信息

目的:探讨加速康复外科(ERAS)理念在肝切除术围手术期中的应用效果。方法:选择2014年2月-2018年12月于阜阳市人民医院行肝切除术的97例患者,按随机序贯原则分为ERAS组(49例)和对照组(48例),ERAS组患者采用ERAS理念行围手术期管理,对照组则按照传统方式行围手术期管理。比较两组患者的相关临床指标。结果:两组患者的一般资料差异无统计学意义(均P>0.05)。与对照组比较,ERAS组患者手术时间明显延长[(186.18±51.31)min vs.(157.00±66.53)min,P=0.02],但术中出血量与无输血率无统计学差异(均P>0.05);术后拔管时间[(6.04±2.09)d vs.(8.44±2.97)d]、肛门排气时间[(1.96±0.79)d vs.(2.52±1.26)d]及术后住院时间[(8.57±3.21)d vs.(11.54±4.82)d]均明显缩短(均P<0.05);术后并发症发生率明显降低(20.4%vs. 60.4%,P=0.001)。结论:ERAS理念在肝切除术治疗肝脏疾病的围术期中应用安全、有效,并可以减少术后并发症的发生,促进患者术后恢复,值得临床应用推广。

关键词：肝切除术肝疾病围手术期加速康复外科

姚魁武教授运用养阴定悸汤治疗室性早搏经验撷英

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天津中医药 2019年第10期36卷 991-993页

作者：吴阳阳姚魁武中国中医科学院广安门医院

室性早搏导致的心悸在临床很为常见,姚魁武教授按照中医理论将其按照“心悸”予以论治。姚魁武教授认为室性早搏所致的心悸与心阳失守、心血失充、心神不藏相关。其病位在心,而不止于心。治疗以“燮理阴阳,安神定悸”为治疗大法,重视宗... 详细信息

室性早搏导致的心悸在临床很为常见,姚魁武教授按照中医理论将其按照“心悸”予以论治。姚魁武教授认为室性早搏所致的心悸与心阳失守、心血失充、心神不藏相关。其病位在心,而不止于心。治疗以“燮理阴阳,安神定悸”为治疗大法,重视宗气、阳气与神志对于心脉的调摄作用。选药处方以大力滋阴补血,兼以温复心阳、安神定悸为务,并根据多年临床经验,以炙甘草汤与桂枝甘草龙骨牡蛎汤合方化裁为养阴定悸汤,并依据证情变化予以进退。

关键词：室性早搏炙甘草汤桂枝甘草龙骨牡蛎汤心悸临床经验

农业院校国家自然科学基金全程质量管理的思考与实践

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科技管理研究 2012年第5期32卷 150-153页

作者：刘双清伍小松王奎武湖南农业大学科技处湖南长沙410128

近年来,国家对基础研究和应用基础研究的支持力度不断增强,国家自然科学基金项目的申报数量也在不断增加,高校科研管理部门在重视项目申报的过程管理、提高申报质量的同时,也要注重项目立项后的中期检查、结题验收、绩效考核和成果转化... 详细信息

近年来,国家对基础研究和应用基础研究的支持力度不断增强,国家自然科学基金项目的申报数量也在不断增加,高校科研管理部门在重视项目申报的过程管理、提高申报质量的同时,也要注重项目立项后的中期检查、结题验收、绩效考核和成果转化等工作。本文结合工作实践与体会,介绍了对国家自然科学基金项目进行前期、中期及后期三个阶段全程质量管理的基本思路和主要做法,以期为农业院校科研管理部门提供有价值的参考和借鉴。

关键词：农业院校国家自然科学基金质量管理