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Detection of H pylori antibody profile in serum by protein array

World Journal of Gastroenterology 2006年第25期12卷 4044-4048页

作者： Feng-chan Han Xu-Jun Li Hong Jiang Li-Peng Qin Ding Li Yan-Hai Guo Zhi-Guang Liu Li Zhang Xiao-Jun Yan Institute of Genetic Diagnosis State Key Laboratory of Cancer Biology The Fourth Military Medical University Xi'an 710032 Shaanxi Province China Department of Gastroenterology Xi'an Central Hospital Xi'an 710032 Shaanxi Province China

AIM： To detect multiple H pylori antibodies in serum samples of individuals who carryHpylori by protein array. METHODS： Recombinant H pylori antigens, urease B subunit （UreB）, vacuolating toxin A （VacA） and cytotoxin associated gene A protein （CagA）, were prepared and immobilized in matrixes on nitrocellulose membrane by robotics to bind the specific immunoglobulin G （IgG） antibodies in serum. Staphylococcus protein A （SPA） labeled by colloid gold was used to integrate the immuno-complex and gave red color signal. The scanner based on charge-coupled device （CCD） could collect the image signal and convert it into digital signal. RESULTS： When human IgG was printed on the membrane in increasing concentrations and incubated with immunogold, a linear dose response curve was obtained and the detection limit for IgG was about 0.025 ng. The cutoff values, which were defined as the mean grey level plus 3 times of standard deviation, were 27.183, 28.546 and 27.402, for anti-UreB IgG, anti- CagA IgG and anti-VacA IgG, respectively, as 400 human serum samples with negative H pylori antibodies were detected by the protein array. When 180 serum samples from patients in hospital were employed for detection of IgG against UreB, CagA and VacA, the sensitivity of the protein array was 93.4%, 95.4%, 96.0%, and the specificity was 94.8%, 94.4% and 97.5%, respectively, as compared with the results obtained by ELISA. The assay also showed high reproducibility, uniformity and stability, and the results were available within 30 min. CONCLUSION： The protein array is a very practical method for rapid detection of multiple antibodies in serum samples. It is especially useful for large scale epidemiological investigation of the infection of Hpylori.

关键词： Helicobacter pylori Protein array Antibody Immunogold

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麦肯锡香港分公司办公室

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城市环境设计 2014年第6期83卷 64-69页

作者： Karbi chan Yin Ho Katja Lam Mike Lim Ted Lin Catherine ng Jesung Park Elaine Tsui Patrizia Zobernig

为满足这所顾问公司的使用需求,OMA设计的麦肯锡香港分公司办公室提供了更私密的空间,增加员工的合作性及归属感。OMA在设计新办公室的时候所遇到的设计问题是:如何重新考虑空间运用,推动麦肯锡的公司文化?OMA的设计师从黑色条码图案获... 详细信息

为满足这所顾问公司的使用需求,OMA设计的麦肯锡香港分公司办公室提供了更私密的空间,增加员工的合作性及归属感。OMA在设计新办公室的时候所遇到的设计问题是:如何重新考虑空间运用,推动麦肯锡的公司文化?OMA的设计师从黑色条码图案获得灵感,来设计新办公室的布局——各个功能空间组织成横条形状,并列在整个办公室里。新设计的办公室摆脱了传统写字楼的模式,

关键词：归属感香港分公司麦肯锡使用状态

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Roux-en-Y reconstruction does not require gastric decompression after radical distal gastrectomy

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World Journal of Gastroenterology 2012年第3期18卷 251-256页

作者： Cheng-Jueng Chen Tsang-Pai Liu Jyh-Cherng Yu Sheng-Der Hsua Tsai-Yuan Hsieh Heng-Cheng Chu Chung-Bao Hsieh Teng-Wei Chen, De-Chuan chan Division of General Surgery Tri-Service General Hospital National Defense Medical Center 11490 Taipei Taiwan China Department of Surgery Mackay Memorial Hospital Mackay Medicine Nursing and Management College Peitou 10449 Taipei Taiwan China Division of Gastroenterology Tri-Service General Hospital National Defense Medical Center 11490 Taipei Taiwan China

AIM: To determine whether routine nasogastric (ng) decompression benefitted patients undergoing radical gastric surgery. METHODS: Between January 1998 and December 2008, 519 patients who underwent distal gastrectomy for gastric cancer were retrospectively divided into 2 time-period cohorts; those treated with Billroth Ⅱ (BⅡ) reconstruction in the first 6 years and those with Roux-en-Y (RY) reconstruction in the last 5 years. In the latter group, the patients were further divided into 2 subgroups; with and without nasogastric ***: Postoperatively, there were no significant differences in the number of anastomotic leaks between the 3 groups. In the tubeless RY group, time to semiliquid diet was significantly shorter than in the other 2 groups (4.4 d ± 1.4 d vs 7.2 d ± 1.3 d and 5.9 d ± 1.2 d, P = 0.005). The length of postoperative stay was significantly increased in patients with BⅡ reconstruction compared with patients with RY reconstruction with/without ng decompression (15.4 d ± 4.3 d in BⅡ group vs 12.6 d ± 3.1 d in decompressed RY and 11.4 d ± 3.4 d in the tubeless RY group, P = 0.035). The postoperative pneumonia rate was lowest in the tubeless group and highest in the BⅡ group (1.4% vs 4.6%, P = 0.01). Severe sore throat was noted in 59 (20.7%) members of the BⅡ group, 18 (17.4%) members of the decompressed RY group and 6 (4.2%) members of the tubeless RY group. Fewer patients in the tubeless group complained of severe sore throat (P = 0.001). CONCLUSION: This study provides support for abandoning routine ng decompression in patients undergoing subtotal gastrectomy with Roux-en-Y gastrojejunostomy.

关键词： Nasogastric decompression Billroth Ⅱ gas- trojejunostomy Roux-en-Y gastrojejunostomy Radical distal gastrectomy Gastric cancer

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Effects of Korean ginseng berry extract （GB0710） on penile erection： evidence from in vitro and in vivo studies

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Asian Journal of Andrology 2013年第4期15卷 503-507,I0008页

作者： Kang Su Cho chan Woong Park Chun-Ki Kimt Hee Young Jeon Wan Gi Kim Sang Jun Lee Young-Myeong Kim Joo Yong Lee Young Deuk Choi Department of Urology Severance Hospital Urological Science Institute Yonsei University College of Medicine Seou1120-752 Korea R&D Center Amorepacific Corporation Yongin Gyeonggi-do 446-729 Korea Vascular System Research Center and Department of Molecular and Cellular Biochemistry School of Medicine Kangwon National University Chunchon 200-701 Korea and Clinical Trial Center for Medical Devices Yonsei University College of Medicine Seoul 120-752 Korea

Several reports have promoted the root-derived Korean red ginseng （KRG; Panax ginseng） as alternative treatment for erectile dysfunction （ED）, and ginsenosides are known to be the principal active ingredients of ginseng. Recent studies showed that ginseng berries produce more ginsenosides than KRG; thus, we investigated the ability of the Korean ginseng berry extract GB0710 to relax the penile corpus cavernosum smooth muscle （CCSM） in this study. As a comparative control, the results were compared to those obtained using KRG. In addition, possible mechanisms of action for GB0710 were investigated. While KRG and GB0710 both displayed dose-dependent relaxation effects on precontracted rabbit CCSM in vitro, GB0710 was shown to be more potent than KRG. The GBO710-induced relaxation could be partially reduced by removing the endothelium. In addition, pre-treatment with several nitric oxide （NO） inhibitors significantly inhibited the relaxation of muscle strips. Furthermore, administration of GB0710 increased intracavernosal pressure （ICP） in a rat in vivo model in both a dose- and duration-dependent manner. Intracellular NO production in human microvascular endothelial cells could be induced by GB0710 and inhibited by ng-monomethyI-L-arginine. In conclusion, GB0710 had a greater relaxation effect on rabbit CCSM than did KRG extract, and increased ICP in a rat model in both a dose- and a duration-dependent manner. This relaxine effect might be mediated bv NO production.

关键词： corpus cavernosum erectile dysfunction (ED) ginseng berry nitric oxide (NO) smooth muscle

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Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits

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World Journal of Stem Cells 2019年第12期11卷 1115-1129页

作者： Kyungha Shin Yeseul Cha Young-Hwan Ban Da Woom Seo Ehn-Kyoung Choi Dongsun Park Sung Keun Kang Jeong chan Ra Yun-Bae Kim College of Veterinary Medicine Chungbuk National UniversityCheongju 28644ChungbukSouth Korea Department of Biology Education Korea National University of EducationCheongju 28173ChungbukSouth Korea Biostar Stem Cell Research Institute R-BIO Co.Ltd.Seoul 07238South Korea

BACKGROUND Osteoarthritis(OA),a chronic age-related disease characterized by the slowly progressive destruction of articular cartilage,is one of the leading causes of *** a new strategy for treatment of OA,mesenchymal stem cells(MSCs)have the potential for articular cartilage ***,thrombospondin 2(TSP2)promotes the chondrogenic differentiation of *** To investigate whether TSP2 induces chondrogenic differentiation of human adipose-derived MSCs(hADMSCs)and potentiates the therapeutic effects of hADMSCs in OA *** We investigated the chondrogenic potential of TSP2 in hADMSCs by analyzing the expression of chondrogenic markers as well as NOTCH signaling genes in normal and TSP2 small interfering RNA(siRNA)-treated stem *** cruciate ligament transection surgery was performed in male New Zealand white rabbits,and 8 wk later,hADMSCs(1.7×10^6 or 1.7×10^7 cells)were injected into the injured knees alone or in combination with intra-articular injection of TSP2(100 ng/knee)at 2-d *** progression was monitored by gross,radiological,and histological *** In hADMSC culture,treatment with TSP2 increased the expression of chondrogenic markers(SOX9 and collagen Ⅱ)as well as NOTCH signaling genes(JAGGED1 and NOTCH3),which were inhibited by TSP2 siRNA *** vivo,OA rabbits treated with hADMSCs or TSP2 alone exhibited lower degree of cartilage degeneration,osteophyte formation,and extracellular matrix loss 8 wk after cell ***,such cartilage damage was further alleviated by the combination of hADMSCs and *** addition,synovial inflammatory cytokines,especially tumor-necrosis factor-α,markedly decreased following the combination *** The results indicate that TSP2 enhances chondrogenic differentiation of hADMSCs via JAGGED1/NOTCH3 signaling,and that combination therapy with hADMSCs and TSP2 exerts synergistic effects in the cartilage regeneration of OA joints.

关键词： Osteoarthritis Anterior cruciate ligament transection Human adipose tissuederived mesenchymal stem cell Thrombospondin 2 Notch signaling Cartilage regeneration

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Characteristics and source regions of the biomass burning tracer levoglucosan in spring at four background sites in South China

Characteristics and source regions of the biomass burning tr...

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第七届亚洲气溶胶会议

作者： Chuen-Yu chan Guenter Engling School of Environmental Science and Engineering Sun Yat-sen University National Tsing Hua University Department of Biomedical Engineering and Environmental Science

An intensive field study was carried out to identify the influence of biomass burning and the associated source regions during spring in South China. Ambient concentrations of the biomass burning tracer levoglucosan were determined at four background stations: Jianfengling （JFL） on Hainan Island, Hok Tsui （HT） in Hong Kong, Lin’an （LA） in Zhejiang province and Tengchong （TC） in Yunnan province. Exceptionally high levels of levoglucosan were found in TC, with an average concentration of 1070 ng m-3, while those at the other sites were lower by two orders of magnitude. Elevated levoglucosan levels were further investigated with the help of fire hot spot counts and aerosol index derived from satellite data, as well as backward trajectories. Southwest Asia, the Philippines and the Southeast China coast area were identified as source regions of biomass burning aerosol transported to South China. Using a simplified receptor-based model, the contribution of biomass burning to OC was estimated to be as high as 73% at the TC site due to strong influence by biomass burning activities in Southwest Asia. Moreover, the widespread biomass burning smoke plumes derived from the Philippines were reported for the first time in this study, accounting for 21% and 4.5% of OC in PM2.5 at JFL and HT, respectively.

关键词： Biomass combustion Levoglucosan Source apportionment Long-range transport

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CAPD病人腹部疝并发症

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中华肾脏病杂志 1988年第4期04卷 219-220,F4页

作者：陈静英吴小凤李文骥陈文岩香港大学内科东华医院透析中心香港大学内科港大外科香港大学内科港大内科

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新型冠狀病毒感染住院患者病毒載量與炎症指標的分析

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镜湖医学 2023年第1期23卷 25-27,90,I0001页

作者：馮婉珊吳健欣吳許文陳倩碧陳浩雲澳門鏡湖醫院檢驗科中國澳門

目的探討新冠肺炎住院患者其病毒載量與C反應性蛋白(CRP)、鐵蛋白(Ferritin)和白介素-6(IL-6)的關係。方法本研究納入2023年1月~2023年4月在本院收治入院的46例新冠肺炎患者。根據臨床分型標準分為輕症組(25例)和重症組(21例),分別檢測... 详细信息

目的探討新冠肺炎住院患者其病毒載量與C反應性蛋白(CRP)、鐵蛋白(Ferritin)和白介素-6(IL-6)的關係。方法本研究納入2023年1月~2023年4月在本院收治入院的46例新冠肺炎患者。根據臨床分型標準分為輕症組(25例)和重症組(21例),分別檢測入院後患者的新冠核酸Ct值、CRP、Ferritin及IL-6水平,統計分析以上指標與疾病程度的關係。結果在46例新冠患者中結果表明,重症組病毒載量(28.83±5.63)、CRP[76.40(23.08,115.00)]及IL-6[43.10(26.60,86.10)]顯著高於輕症組病毒載量(33.88±2.03)、CRP[13.70(6.56,50.60)]和IL-6[13.60(5.97,26.20)](P<0.001);受試者工作特徵曲線(ROC)分析結果顯示,CRP和IL-6指標可用於診斷新冠肺炎疾病的嚴重程度(AUC分別為0.75和0.76);相關性分析發現CRP和IL-6與核酸Ct值呈負相關(rs=-0.419,P=0.004;rs=-0.373,P=0.011);CRP與IL-6呈顯著正相關(rs=0.706,P=0.000);未發現鐵蛋白在兩組間有差異以及與核酸Ct值的相關性。結論本研究通過對住院病人進行分析,發現其病毒載量越高的患者,更容易出現嚴重病情,因此,持續檢測CRP和IL-6水平可以用來監測患者病情進展。

关键词：新型冠狀病毒感染新型冠狀病毒載量 C反應性蛋白鐵蛋白白介素-6

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Validated model for prediction of recurrent hepatocellular carcinoma after liver transplantation in Asian population

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World Journal of Gastrointestinal Oncology 2019年第4期11卷 322-334页

作者： Ka Wing Ma Wong Hoi She Albert Chi Yan chan Tan To Cheung James Yan Yue Fung Wing Chiu Dai Chung Mau Lo Kenneth Siu Ho Chok Department of Surgery the University of Hong Kong Department of Surgery and State Key Laboratory for Liver Research the University of Hong Kong Department of Medicine and State Key Laboratory for Liver Research The University of Hong Kong

BACKGROUND Liver transplantation(LT) is regarded as the best treatment for both primary and recurrent hepatocellular carcinoma(HCC). Post-transplant HCC recurrence rate is relatively low but significant, ranging from 10%-30% according to different series. When recurrence happens, it is usually extrahepatic and associated with poor prognosis. A predictive model that allows patient stratification according to recurrence risk can help to individualize post-transplant surveillance protocol and guidance of the use of anti-tumor immunosuppressive *** To develop a scoring system to predict HCC recurrence after LT in an Asian *** Consecutive patients having LT for HCC from 1995 to 2016 at our hospital were recruited. They were randomized into the training set and the validation set in a60:40 ratio. Multivariable Cox regression model was used to identity factors associated with HCC recurrence. A risk score was assigned to each factor according to the odds ratio. Accuracy of the score was assessed by the area under the receiver operating characteristic *** In total, 330 patients were eligible for analysis(183 in training and 147 invalidation). Recurrent HCC developed in 14.2% of them. The median follow-up duration was 65.6 mo. The 5-year disease-free and overall survival rates were78% and 80%, respectively. On multivariate analysis, alpha-fetoprotein > 400 ng/mL [P = 0.012, hazard ratio(HR) 2.92], sum of maximum tumor size and number(P = 0.013, HR 1.15), and salvage LT(P = 0.033, HR 2.08) were found to be independent factors for disease-free survival. A risk score was calculated for each patient with good discriminatory power(c-stat 0.748 and 0.85, respectively,in the training and validation sets). With the derived scores, patients were classified into low-(0–9), moderate-(> 9–14), and high-risk groups(> 14), and the risk of HCC recurrence in the training and validation sets was 10%, 20%, 54%(cstat 0.67) and 4%, 22%, 62%(c-stat 0.811), accordin

关键词： Hepatocellular carcinoma Liver transplantation Post-transplant recurrence Predictive model

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HYPOPIGMENTATION AND HEALTHY SKIN PROMOTIng EFFECT OF A MODIFIED QI BAI SAN

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World Journal of Traditional Chinese Medicine 2016年第2期 81-82页

作者： Elaine Wat Yau Tsz chan Tiffany Hoi Ka Tso Chun Fai ng Ching Po Lau Wing Sum Siu Chun Hay Ko Ka Keung Lau Raymond Hu Clara Bik-San Lau Ping Chung Leung Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and Plant Resources in West China The Chinese University of Hong Kong 5100 Cosmetic Company Ltd.

Introduction:Qi Bai San(QBS,七白散)is a traditional Chinese herbal formula used by ancient ladies for healthy skin and *** it is a popular product in the market,it contains animal drug and toxic herbs which

关键词： MSH HYPOPIGMENTATION AND HEALTHY SKIN PROMOTIng EFFECT OF A MODIFIED QI BAI SAN

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