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Cancer Biology & Medicine 2024年第2期21卷 175-192页

作者： Lulu Han Yuxin Chen Nan Huang Xiaowan Zhou Yanfang Lv Huizhong Li Dafei Chai Junnian Zheng gang Wang Cancer Institute Xuzhou Medical University209 Tongshan RoadXuzhou 221004China Center of Clinical Oncology The Affiliated Hospital of Xuzhou Medical University99 West Huaihai RoadXuzhou 221002China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Xuzhou Medical University209 Tongshan RoadXuzhou 221004China Department of Oncology Suzhou Xiangcheng People’s HospitalSuzhou 215131China

Objective:Neutrophils are one of the most predominant infiltrating leukocytes in lung cancer tissues and are associated with lung cancer *** neutrophils promote lung cancer progression,however,has not been ***:Kaplan–Meier plotter online analysis and tissue immunohistochemistry were used to determine the relationship between neutrophils and overall survival in lung cancer *** effect of neutrophils on lung cancer was determined using the Transwell migration assay,a proliferation assay,and a murine tumor *** knockdown was used to determine poly ADPribose polymerase(PARP)-1 function in lung cancer-educated *** blot analysis and gelatin zymography were used to demonstrate the correlation between PARP-1 and matrix metallopeptidase 9(MMP-9).Immunoprecipitation coupled to mass spectrometry(ip/MS)was used to identify the proteins interacting with ***-immunoprecipitation(Co-ip)was used to confirm that PARP-1 interacts with arachidonate 5-lipooxygenase(ALOX5).Neutrophil PARP-1 blockage by AG14361 rescued neutrophil-promoted lung cancer ***:An increased number of infiltrating neutrophils was negatively associated with overall survival in lung cancer patients(P<0.001).Neutrophil activation promoted lung cancer cell invasion,migration,and proliferation in vitro,and murine lung cancer growth in ***,PARP-1 was shown to be involved in lung cancer cell-induced neutrophil activation to increase MMP-9 expression through interacting and stabilizing ALOX5 by post-translational protein modification(PARylation).Blocking PARP-1 by gene knockdown or AG14361 significantly decreased ALOX5 expression and MMP-9 production,and eliminated neutrophil-mediated lung cancer cell invasion and in vivo tumor ***:We identified a novel mechanism by which PARP-1 mediates lung cancer cell-induced neutrophil activation and PARylates ALOX5 to regulate MMP-9 expression,which exacerbates lung cancer

关键词： Lung cancer neutrophils PARP-1 ALOX5 MMP-9

Biochar regulates biogeochemical cycling of iron and chromium in a soil-rice system by stimulating Geobacter and Clostridium

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Pedosphere 2024年第5期34卷 929-940页

作者： Min XU Yang LIN Jing MA Lulu LONG Chao CHEN gang YANG Chun SONG Jun WU Xiaohong ZHANG Peng GAO College of Environmental Science Sichuan Agricultural University Soil Water and Ecosystem Sciences Department University of Florida College of Water Conservancy and Hydropower Engineering Sichuan Agricultural University Department of Environmental and Occupational Health University of Pittsburgh School of Public Health

In soil-rice systems,microbial reduction of iron （Fe） has been recognized as a crucial biogeochemical process that regulates Fe and chromium （Cr）translocation;however,the underlying processes are *** investigate the impacts of biochar on the biochemical cycling of Fe and Cr and their toxicity to rice,maize straw biochar was applied at 1%（weight/weight） to a paddy soil spiked with 300 mg kg-1Cr under two phosphorus （P） levels (0 or 90 mg kg-1) in a pot *** key microbial groups affecting Fe dissimilatory reduction and their environmental drivers were *** inhibited root Cr uptake by 36%,owing to the promoted iron plaque （ip） formation on the rice root *** analysis showed that Fe concentration in pore water was strongly linked to the abundances of Geobacter （r=0.81–0.94,Pip formation and altering subcellular *** mechanistic insights had important implications for reducing Cr uptake by rice.

关键词： chromium translocation iron plaque iron reduction maize straw biochar microbial reduction paddy soil phosphorus fertilization subcellular distribution

Unveiling the Role of Acetate in Probiotic-Mediated Promotion of Intestinal Serotonin Production Using Intestinal Organoids

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Food Science and Human Wellness 2024年

作者： Peijun Tian Zheng Wang Xin Qian Jianxin Zhao gang Wang Wei Chen State Key Laboratory of Food Science and Resources， Jiangnan University School of Food Science and Technology， Jiangnan University National Engineering Research Center for Functional Food， Jiangnan University (Yangzhou) Institute of Food Biotechnology， Jiangnan University

The neurotransmitter 5-hydroxytryptamine （5-HT）， primarily produced by intestinal enterochromaffin cells （EC）， relies on Tryptophan hydroxylase （TPH1） for synthesis. Research suggested Bifidobacterium breve CCFM1025's potential in regulating Tph1 gene expression， maintaining 5-HT levels in stressed mice， but its precise mechanisms were unclear. This study used metabolomic techniques to assess probiotic fermentation products， revealing acetate as the crucial element in Bb-CCFM1025's regulation of gut 5-HT synthesis. Further exploration correlated acetate with Tph1 transcription in intestinal organoids. Transcriptomic methods and qRT-PCR validation demonstrated how acetate facilitated 5-HT synthesis and secretion. It unveiled that acetate orchestrates signaling pathways (PI3K-AKT， PLC-pERK， and PLC-ip3-Ca2+) within EC cells， enabling 5-HT production. These findings elucidate the biochemical mechanisms behind specific probiotics' effects， aiding in the targeted selection of similar beneficial strains. This study offers theoretical support for choosing probiotics with analogous functionalities based on their physiological impacts.

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四川网络文学ip孵化与影视改编策略分析

电影评介 2024年第11期 88-94页

作者：李正丽王钢四川电影电视学院

依托强大的故事叙述力、跨媒介的内容延展力、深厚的粉丝影响力,网络文学正以丰富饱满的内容和创新多元的媒介形式,成为刻画社会生活场景、反映时代发展潮流、展现民族精神力量的重要载体。四川盛以其丰富的巴蜀文化滋养了《斗破穹苍》... 详细信息

依托强大的故事叙述力、跨媒介的内容延展力、深厚的粉丝影响力,网络文学正以丰富饱满的内容和创新多元的媒介形式,成为刻画社会生活场景、反映时代发展潮流、展现民族精神力量的重要载体。四川盛以其丰富的巴蜀文化滋养了《斗破穹苍》等众多优秀网络文学作品,拥有庞大的网络作家群。然而,四川网络文学发展也面临着创作人才流失、ip外流现象严重等突出问题。在成渝地区双城经济圈建设战略和媒介融合的时代潮流下,川渝两地应加强区域合作,聚焦精品创作生产,借鉴杭州网络文学之都等建设经验,从人才引留、精品创作、平台建设和文旅融合等方面积极作为。

关键词：网络文学 ip孵化影视改编

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激电测深法在勘探采空区中的应用和分析

世界有色金属 2024年第2期 100-102页

作者：徐刚安徽省地质矿产勘查局322地质队安徽马鞍山243000

激电测深法是一种快速有效、经济无损探查地下采空区的一种地球物理勘探方法,主要通过人工地下直流电场激发,以电测深装置形式,接收、研究地下横、纵向激发极化效应变化的来划分地层和圈定地下采空区位置范围。本文根据工程实例对该方... 详细信息

激电测深法是一种快速有效、经济无损探查地下采空区的一种地球物理勘探方法,主要通过人工地下直流电场激发,以电测深装置形式,接收、研究地下横、纵向激发极化效应变化的来划分地层和圈定地下采空区位置范围。本文根据工程实例对该方法的技术原理及后期的数据处理推断解释进行研究,分析激电测深在马鞍山市和睦山充填站区探查地下采空区范围的应用,为地下采空区处理和周边边坡的稳定性分析提供科学的依据。

关键词：激电测深法视电阻率视极化率采空区

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雷达对抗中基于FPGA输入分块重映射的数据处理方法

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导弹与航天运载技术（中英文） 2024年第2期 86-90,106页

作者：薛晓琴孟刚陈旸李宏博赵健北京航天长征飞行器研究所北京100076 试验物理与计算数学国家重点实验室北京100076 哈尔滨工业大学哈尔滨150006

雷达对抗中,干扰机侦收雷达的信号,经AD采样后输入FPGA进行处理并产生干扰信号。FPGA平台利用Xilinx等官方提供的FFTip核对高速ADC输出的数据进行FFT处理时,需要进行“并行-串行”的数据预处理,降低了处理速度,同时也没有对FPGA内部资... 详细信息

雷达对抗中,干扰机侦收雷达的信号,经AD采样后输入FPGA进行处理并产生干扰信号。FPGA平台利用Xilinx等官方提供的FFTip核对高速ADC输出的数据进行FFT处理时,需要进行“并行-串行”的数据预处理,降低了处理速度,同时也没有对FPGA内部资源进行最大化利用。基于以上问题,提出一种基于FPGA输入分块重映射的数据处理方法。该方法通过数据输入重映射模块将输入雷达数据优化为并行分块的数据流格式,再通过FFT蝶形网络输出离散傅里叶变换数据,最后进行并行结果输出。试验证明,本方法能有效节省FPGA的运算时间,提高雷达数据处理速度,优化利用了FPGA内部资源,具有实时性好、灵活性高的特点。

关键词：雷达对抗 FPGA 分块重映射数据处理蝶形网络

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唑吡坦对低压低氧致失眠模型小鼠的镇静催眠作用

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中国药理学与毒理学杂志 2024年第2期38卷 81-88页

作者：梁欢欢许琳俞纲苏瑞斌李明媛天津科技大学生物工程学院天津市透明质酸应用研究企业重点实验室天津300457 军事科学院军事医学研究院毒物药物研究所抗毒药物与毒理学国家重点实验室北京100850

目的研究唑吡坦的镇静催眠作用及对丘脑和下丘脑氨基酸类神经递质含量的影响。方法模拟海拔5500 m低压低氧环境,通过阈上剂量(50 mg·kg^(-1),ip)戊巴比妥钠诱导小鼠翻正反射消失(LORR)实验建立低压低氧致失眠模型。采用LORR实验观察常... 详细信息

目的研究唑吡坦的镇静催眠作用及对丘脑和下丘脑氨基酸类神经递质含量的影响。方法模拟海拔5500 m低压低氧环境,通过阈上剂量(50 mg·kg^(-1),ip)戊巴比妥钠诱导小鼠翻正反射消失(LORR)实验建立低压低氧致失眠模型。采用LORR实验观察常压常氧和低压低氧条件下唑吡坦(0.33,1,3,9和27 mg·kg^(-1),ip)与戊巴比妥钠镇静催眠阈下(20 mg·kg^(-1),ip)和阈上剂量的协同效应及唑吡坦(10,13,17,20,23,30和40 mg·kg^(-1),ip)的镇静催眠效应。运用高效液相荧光检测法测定常压常氧和低压低氧条件下给予唑吡坦(10,20和40 mg·kg^(-1),ip)1 h后,小鼠丘脑和下丘脑谷氨酸(Glu)和γ-氨基丁酸(GABA)含量。结果低压低氧处理1 d显著缩短阈上剂量戊巴比妥钠诱导小鼠LORR持续时间(P<0.05)。与常压常氧溶媒组和低压低氧致失眠溶媒组相比,常压常氧唑吡坦9和27 mg·kg^(-1)组和低压低氧致失眠模型+唑吡坦9和27 mg·kg^(-1)组均显著缩短阈下及阈上剂量戊巴比妥钠诱导LORR的潜伏期(P<0.01,P<0.05),同时显著延长LORR持续时间(P<0.01,P<0.05);常压常氧唑吡坦组和低压低氧致失眠模型+唑吡坦组致小鼠LORR的半数有效剂量(ED50)分别为16.21和20.55 mg·kg^(-1)。神经递质水平检测结果表明,与常压常氧溶媒组相比,常压常氧唑吡坦40 mg·kg^(-1)组显著降低丘脑和下丘脑Glu含量及下丘脑Glu/GABA比值(P<0.01,P<0.05);与常压常氧溶媒组相比,低压低氧致失眠模型组小鼠下丘脑Glu含量及Glu/GABA比值显著升高(P<0.01,P<0.05),低压低氧致失眠模型+唑吡坦40 mg·kg^(-1)组显著逆转其升高(P<0.05)。结论在低压低氧条件下,唑吡坦的镇静催眠效应减弱,唑吡坦对下丘脑中Glu和GABA水平的调节可能在其镇静催眠效应中发挥重要作用。

关键词：低压低氧唑吡坦翻正反射谷氨酸 γ-氨基丁酸

Gastric motor effects of ghrelin and growth hormone releasing peptide 6 in diabetic mice with gastroparesis

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World Journal of Gastroenterology 2008年第9期14卷 1419-1424页

作者： Wen-Cai Qiu Zhi-gang Wang Wei-gang Wang Jun Yan Qi Zheng The Affiliated Sixth Hospital of Medical School Shanghai Jiaotong University Shanghai 200233 China

AIM:To investigate the potential therapeutic significance of ghrelin and growth hormone releasing peptide 6 (GHRP-6) in diabetic mice with gastric motility disorders. METHODS: A diabetic mouse model was established by intraperitoneal (ip) injection of alloxan. Diabetic mice were injected ip with ghrelin or GHRP-6 (20-200 μg/kg), and the effects on gastric emptying were measured after intragastric application of phenol red. The effect of atropine, NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) or D-Lys3-GHRP-6 (a growth hormone secretagogue receptor (GHS-R) antagonist) on the gastroprokinetic effect of ghrelin or GHRP-6 (100 μg/kg) was also investigated. The effects of ghrelin or GHRP-6 (0.01-10 μmol/L) on spontaneous or carbachol-induced contractile amplitude were also investigated in vitro, in gastric fundic circular strips taken from diabetic mice. The presence of growth hormone secretagogue receptor 1a transcripts in the fundic strips of diabetic mice was detected by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: We established a diabetic mouse model with delayed gastric emptying. Ghrelin and GHRP-6 accelerated gastric emptying in diabetic mice with gastroparesis. In the presence of atropine or L-NAME, which delayed gastric emptying, ghrelin and GHRP-6 (100 μg/kg) failed to accelerate gastric emptying. D-Lys3-GHRP-6 also delayed gastric emptying induced by the GHS-R agonist. Ghrelin and GHRP-6 increased the carbachol-induced contractile amplitude in gastric fundicstrips taken from diabetic mice. RT-PCR confirmed the presence of GHS-R mRNA in the strip preparations. CONCLUSION: Ghrelin and GHRP-6 increase gastric emptying in diabetic mice with gastroparesis, perhaps by activating peripheral cholinergic pathways in the enteric nervous system.

关键词： Gastric emptying Ghrelin Growth hormone releasing peptide 6 Growth hormone secretagogue receptor Diabetic mice

RISP： An RPKI-Based Inter-AS Source Protection Mechanism

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Tsinghua Science and Technology 2018年第1期23卷 1-12页

作者： Yihao Jia Ying Liu gang Ren Lin He Institute for Network Sciences and Cyberspace Tsinghua University Beijing 100084 China

ip source address spoofing is regarded as one of the most prevalent components when launching an anonymous invasion, especially a Distributed Denial-of-Service （DDoS） attack. Although Source Address Validations （SAVs） at the access network level are standardized by the Internet Engineering Task Force （iETF）, SAV at the inter-Autonomous System （AS） level still remains an important issue. To prevent routing hijacking, the IETF is constructing a Resource Public Key Infrastructure （RPKI） as a united trust anchor to secure interdomain routing, in this study, we creatively use the RPKI to support inter-AS SAV and propose an RPKI-based Inter-AS Source Protection （RISP） mechanism. According to the trust basis provided by the RPKI, RISP offers ASes a more credible source-oriented protection for the ip addresses they own and remains independent of the RPKI. Based on the experiments with real Internet topology, RISP not only provides better incentives, but also improves efficacy and economizes bandwidth with a modest resource consumption.

关键词： ip spoofing source address validation inter-AS RPKI DDoS