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Expression and Antigenic Characterization of the Epitope-G_1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris

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中国病毒学 2007年第5期22卷 347-352页

作者： Fu-ying ZHENG Guo-zhen LIN Chang-qing QIU kui-zhang yuan Jun-ying SONG Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046China Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 China

The epitope-G1 gene of Bovine ephemeral fever virus(BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid *** the pPIC9K-G1 was linearized and transformed into Pichia pastoris *** recombinant *** strains were selected by a G418 transformation screen and confirmed by *** being induced with methanol,an expressed protein with 26 kDa molecular weight was obtained,which was much bigger than the predicted size(15.54 kDa).Deglycosylation analysis indicated the recombinant G1 was *** blot and ELISA tests,as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and *** recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.

关键词：基因表达转基因抗原-G1 牛流行热病毒甲醇酵母

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ESTERIFICATION REACTION OF ACETIC ACID WITH n-AMYL ALCOHOL OVER ZEOLITEBETA

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Journal of Energy Chemistry 1998年第4期 59-68+91页

作者： zhang Huai Bin\ \ zhang kui\ \ yuan Zhong Yong ZHAO Wei\ \ LI He Xuan Department of Chemistry, Nankai University Tianjin \ 300071 南开大学化学系

在连续流动反应装置上研究了β沸石分子筛上正戊醇的酯化反应。经５００℃焙烧后的β分子筛对该反应有较好的催化作用。在β分子筛上，适宜的反应温度范围是１４０～１５０℃，较高的反应温度将降低催化剂的活性与选择性。随着乙酸／正... 详细信息

在连续流动反应装置上研究了β沸石分子筛上正戊醇的酯化反应。经５００℃焙烧后的β分子筛对该反应有较好的催化作用。在β分子筛上，适宜的反应温度范围是１４０～１５０℃，较高的反应温度将降低催化剂的活性与选择性。随着乙酸／正戊醇摩尔比和ＬＨＳＶ的增加，酯的选择性增加。文中还讨论了离子交换度和焙烧温度对β分子筛表面性质和催化性能的影响。

关键词： β沸石酯化反应正戊醇乙酸

Silurian integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

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Science China Earth Sciences 2024年第4期67卷 1005-1035页

作者： Zhongyang CHEN Qing CHEN Guangxu WANG Xiang FANG Peng TANG Guanzhou YAN Wenwei yuan Bing HUANG Xiaole zhang kui YAN yuandong zhang Yi WANG State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China

The Silurian palaeontology and stratigraphy of the Qinghai-Tibetan Plateau have been studied for more than100 *** the launch of the Second Tibetan Plateau Scientific Expedition and Research,it is necessary to update the summaries of the Silurian stratigraphy and fossil assemblages of the Qinghai-Tibetan Plateau and their correlation with its surrounding *** this study,we have selected 33 sections from the Qinghai-Tibetan Plateau and its surroundings areas,summarised the available data in terms of stratigraphic regionalisation,lithostratigraphy,chronostratigraphy,and biostratigraphy,and divided the study area into four stratigraphic provinces:the Xizang-Western Yunnan-Western Sichuan Province,the KunlunQilian Province,the South China Province,and the Tarim *** general,the Silurian strata in the Qinghai-Tibetan Plateau is more complete than those in other areas of *** studies of the vertebrate faunas,sporomorph assemblages,and brachiopod faunas of the study area indicate a closer geographical relationship between the South China,Tarim,and Indochina palaeoplates,than previously *** with the surrounding areas where Silurian strata have been extensively studied,the study of Silurian strata in the main part of the Qinghai-Tibetan Plateau is still in the preliminary *** the future,it is necessary to conduct research on the age,distribution,and resource potential of basal Silurian black shales to provide new directions for shale gas exploration and development in *** shallow marine strata of the middle to upper Silurian require further *** that the Silurian System in the Qinghai-Tibetan Plateau is more complete,it has the potential to supplement and improve the study of the carbon isotope stratigraphy of the Silurian in China.

关键词： Qinghai-Tibetan Plateau Silurian Stratigraphic regionalisation Lithostratigraphy Biostratigraphy Palaeogeographical evolution

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Oxygen reduction electrocatalysis:From conventional to single-atomic platinum-based catalysts for proton exchange membrane fuel cells

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Frontiers in Energy 2024年第2期18卷 206-222页

作者： Cheng yuan Shiming zhang Jiujun zhang Institute for Sustainable Energy/College of Sciences Shanghai UniversityShanghai 200444China

Platinum(Pt)-based materials are still the most efficient and practical catalysts to drive the sluggish kinetics of cathodic oxygen reduction reaction(ORR)in proton exchange membrane fuel cells(PEMFCs).However,their catalysis and stability performance still need to be further improved in terms of corrosion of both carbon support and Pt catalyst particles as well as Pt loading *** on the developed synthetic strategies of alloying/nanostructuring Pt particles and modifying/innovating supports in developing conventional Pt-based catalysts,Pt single-atom catalysts(Pt SACs)as the recently burgeoning hot materials with a potential to achieve the maximum utilization of Pt are comprehensively reviewed in this *** design thoughts and synthesis of various isolated,alloyed,and nanoparticlecontained Pt SACs are *** single-atomic Pt coordinating with non-metals and alloying with metals as well as the metal-support interactions of Pt single-atoms with carbon/non-carbon supports are emphasized in terms of the ORR activity and stability of the *** advance further research and development of Pt SACs for viable implementation in PEMFCs,various technical challenges and several potential research directions are outlined.

关键词： oxygen reduction electrocatalysis Pt singleatom catalysts conventional Pt-based catalysts design thoughts and synthesis metal-support interactions

Recovery of cyanophycin granule polypeptide from activated sludge: carbon source dependence and aggregation-induced luminescence characteristics

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Frontiers of Environmental Science & Engineering 2024年第2期18卷 25-34页

作者： kui Zou Hongyuan Liu Bo Feng Taiping Qing Peng zhang College of Environment and Resources Xiangtan UniversityXiangtan 411105China

In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic *** granule polypeptide(CGP),a recently discovered high value-added biopolymer present in activated sludge,has provided new avenues for the recovery of ***,the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus *** study investigated the synthesis of CGP,polyhydroxyalkanoates(PHA),and alginate-like exopolysaccharides(ALE)in various microbial aggregates under different carbon sources feeding *** results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding ***,biofilm was more conducive to CGP accumulation than floc *** with glucose and methanol,sodium acetate significantly enriched the CGP synthetase-encoding gene(cphAabundance=~17419),resulting in the highest CGP yield(average 107.1 mg/g MLSS)in both biofilm and floc *** study is the first to reported the characteristic fluorescence of CGP(Ex/Em=~360/450 nm)caused by the aggregated luminescence of arginine on the side ***,this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.

关键词： Cyanophycin Polyhydroxyalkanoate Alginate-like exopolysaccharides Bioresource recovery Aggregation-induced luminescence

A mix & act liposomes of phospholipase A2-phosphatidylserine for acute brain detoxification by blood‒brain barrier selective-opening

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1827-1844页

作者： Zinan zhang Wenbin Cao Huanchun Xing Shuai Guo Lijuan Huang Lin Wang Xin Sui kui Lu yuan Luo Yongan Wang Jun Yang State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institutes of Pharmacology and ToxicologyBeijing 100850China Tianjin University of Science and Technology Tianjin 300222China Hebei University of Science and Technology Shijiazhuang 050018China

In the treatment of central nervous system disease,the blood-brain barrier(BBB)is a major obstruction to drug delivery that must be *** this study,we propose a brain-targeted delivery strat-egy based on selective opening of the *** strategy allows some simple bare nanoparticles to enter the brain when mixed with special opening material;however,the BBB still maintains the ability to completely block molecules from passing *** on the screening of BBB opening and matrix delivery mate-rials,we determined that phospholipase A2-catalyzed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine li-posomes can efficiently carry drugs into the brain *** an effective dose,this delivery system is safe,especially with its effect on the BBB being *** mix&act delivery system has a simple structure and rapid preparation,making it a strong potential candidate for drug delivery across the BBB.

关键词： Liposomes Phospholipase A2 Phosphoserine Blood-brainbarrier Selectively open Mix&act

Integration of Multiple Spectral Data via a Logistic Regression Algorithm for Detection of Crop Residue Burned Areas:A Case Study of Songnen Plain,Northeast China

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Chinese Geographical Science 2024年第3期34卷 548-563页

作者： zhang Sumei zhang yuan ZHAO Hongmei College of Mining and Engineering Taiyuan University of TechnologyTaiyuan 030024China Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China

The burning of crop residues in fields is a significant global biomass burning activity which is a key element of the terrestrial carbon cycle,and an important source of atmospheric trace gasses and *** estimation of cropland burned area is both crucial and challenging,especially for the small and fragmented burned scars in *** we developed an automated burned area mapping algorithm that was implemented using Sentinel-2 Multi Spectral Instrument(MSI)data and its effectiveness was tested taking Songnen Plain,Northeast China as a case using satellite image of *** employed a logistic regression method for integrating multiple spectral data into a synthetic indicator,and compared the results with manually interpreted burned area reference maps and the Moderate-Resolution Imaging Spectroradiometer(MODIS)MCD64A1 burned area *** overall accuracy of the single variable logistic regression was 77.38%to 86.90%and 73.47%to 97.14%for the 52TCQ and 51TYM cases,*** comparison,the accuracy of the burned area map was improved to 87.14%and 98.33%for the 52TCQ and 51TYM cases,respectively by multiple variable logistic regression of Sentind-2 *** balance of omission error and commission error was also *** integration of multiple spectral data combined with a logistic regression method proves to be effective for burned area detection,offering a highly automated process with an automatic threshold determination *** method exhibits excellent extensibility and flexibility taking the image tile as the operating *** is suitable for burned area detection at a regional scale and can also be implemented with other satellite data.

关键词： crop residue burning burned area Sentinel-2 Multi Spectral Instrument(MSI) logistic regression Songnen Plain,China

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Insight into the Role and Evidence of Oxygen Vacancies in Porous Single-Crystalline Oxide to Enhance Catalytic Activity and Durability

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Research 2024年第2期2023卷 675-683页

作者： Lingting Ye Jiaming Ma Jie zhang Wen Yin yuanguang Xia kui Xie Key Laboratory of Optoelectronic Materials Chemistry and Physics Fujan Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhouFujian 350002China Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China FuzhouFujian 350108China Advanced Energy Science and Technology Guangdong Laboratory 29 Sanxin North RoadHuizhouGuangdong 116023China Spallation Neutron Source Science Center DongguanGuangdong 523803China

Introducing and stabilizing oxygen vacancies in oxide catalysts is considered to be a promising strategy for improving catalytic activity and ***,we quantitatively create oxygen vacancies in the lattice of porous single-crystallineβ-Ga_(2)O_(3)monoliths by reduction treatments and stabilize them through the long-range ordering of crystal lattice to enhance catalytic activity and *** combination analysis of time-of-flight neutron powder diffraction and extended x-ray absorption fine structure discloses that the preferential generation of oxygen vacancy tends to occur at the site of tetrahedral coordination oxygen ions(O_(Ⅲ)sites),which contributes to the formation of unsaturated Ga-O coordination in the monoclinic *** oxygen vacancies are randomly distributed in lattice even though some of them are present in the form of domain defect in the PSC Ga_(2)O_(3)monoliths after the reduction *** number of oxygen vacancies in the reduced monoliths gives 2.32×10^(13),2.87×10^(13),and 3.45×10^(13)mg^(-1)for the Ga_(2)O_(2.952),Ga_(2)O_(2.895),and Ga_(2)O_(2.880),*** therefore demonstrate the exceptionally high C_(2)H_(4)selectivity of~100%at the C_(2)H_(6)conversion of~37%for nonoxidative dehydrogenation of C_(2)H_(6)to C_(2)H_(4).We further demonstrate the excellent durability even at 620℃for 240 h of continuous operation.

关键词： durability coordination Oxygen

Optimization of biofuel supply chain integrated with petroleum refineries under carbon trade policy

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Frontiers of Chemical Science and Engineering 2024年第3期18卷 113-122页

作者： Wenhui zhang Yiqing Luo Xigang yuan Chemical Engineering Research Center School of Chemical Engineering and TechnologyTianjin UniversityTianjin300350China State Key Laboratory of Chemical Engineering Tianjin UniversityTianjin300350China

The use of fossil fuels results in significant carbon dioxide *** have been increasingly adopted as sustainable alternatives to fossil fuel to address this environmental *** petroleum refineries into biofuel supply chains is an effective approach to mitigating greenhouse gas emissions and improving environmental *** this study,an integrated supply chain optimization framework was established,considering the carbon trade *** addition,a mixed-integer nonlinear programming model was developed to optimize the selection of biomass suppliers,construction of pretreatment plants and biorefineries,integration of petroleum refineries,and selection of transportation routes with the objective of minimizing the total annual *** example is presented to illustrate the applicability of the *** optimization results show that integrating petroleum refineries into biofuel supply chains effectively mitigates carbon *** trade policies can have a direct impact on the total annual cost and carbon emissions of the supply chain.

关键词： renewable energy biofuel supply chain carbon trade policy