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国际医学放射学杂志 2024年第2期 255-255页

作者： D.Kawahara Y.Murakami S.Awane Y.Emoto K.Iwashita h.kubota 王俊

摘要目的基于影像组学和剂量组学特征开发一种多机构预测模型，评估食管鳞状细胞癌（ESCC）接受确定性放疗后的局部缓解。方法局部缓解分为2组（不完全组和完全

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Low temperature-mediated repression and far-red light-mediated induction determine morning FLOWERING LOCUS T expression levels

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Journal of Integrative Plant Biology 2024年第1期66卷 103-120页

作者： hayeon Kim hye Won Kang Dae Yeon hwang Nayoung Lee Akane kubota Takato Imaizumi Young hun Song Department of Agricultural Biotechnology Seoul National UniversitySeoul 08826Korea Department of Biology Ajou UniversitySuwon 16499Korea Plant Genomics and Breeding Institute Seoul National UniversitySeoul 08826Korea Graduate School of Science and Technology Nara Institute of Science and TechnologyIkomaNara 630‐0192Japan Department of Biology University of WashingtonSeattleWA 98195USA Institute of Agricultural Life Sciences Seoul National UniversitySeoul 08826Korea

In order to flower in the appropriate season,plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis(Arabidopsis thaliana)FLOWERING LOCUS T(FT).In Arabidopsis,FT messenger RNA levels peak in the morning and evening under natural long-day conditions(LDs).however,the regulatory mechanisms governing morning FT induction remain poorly *** morning FT peak is absent in typical laboratory LDs characterized by high red:far-red light(R:FR)ratios and constant ***,we demonstrate that ZEITLUPE(ZTL)interacts with the FT repressors TARGET OF EATs(TOEs),thereby repressing morning FT expression in natural *** LDs with simulated sunlight(R:FR=1.0)and daily temperature cycles,which are natural LD-mimicking environmental conditions,FT transcript levels in the ztl mutant were high specifically in the morning,a pattern that was mirrored in the toe1 toe2 double *** night-to-morning temperatures increased the inhibitory effect of ZTL on morning FT expression by increasing ZTL protein levels early in the ***-red light counteracted ZTL activity by decreasing its abundance(possibly via phytochrome A(phyA))while increasing GIGANTEA(GI)levels and negatively affecting the formation of the ZTL-GI complex in the ***,the phyA-mediated high-irradiance response and GI play pivotal roles in morning FT *** findings suggest that the delicate balance between low temperature-mediated ZTL activity and the far-red light-mediated functions of phyA and GI offers plants flexibility in fine-tuning their flowering time by controlling FT expression in the morning.

关键词： far-red light FLOWERING LOCUS T flowering time GIGANTEA low night-to-morning temperature natural long days phytochrome A red to far-red ratio ZEITLUPE

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从羰基纤维素和过氧化氢制得纤维素过氧化物

国外纺织技术(化纤、染整、三废治理分册) 1981年第12期 11-14页

作者： h.kubota 张文光

引言本文作者以前曾论述过,纤维素与含有羧基和醛基的聚乙烯醇衍生物的基质用过氧化氢（h2O2）处理可引入过氧化基团,结果所得过氧化基团足能引发热和光接枝。聚合物基质上的羧基和醛基想来就会对 h2O2反应,分别生成过酸和α—羟基过氧... 详细信息

引言本文作者以前曾论述过,纤维素与含有羧基和醛基的聚乙烯醇衍生物的基质用过氧化氢（h2O2）处理可引入过氧化基团,结果所得过氧化基团足能引发热和光接枝。聚合物基质上的羧基和醛基想来就会对 h2O2反应,分别生成过酸和α—羟基过氧化氢型的过氧化物。

关键词：过氧化物热分解分解反应双氧水过氧化氢纤维素

Microbial characteristics in anaerobic membrane bioreactor treating domestic sewage:Effects of hRT and process performance

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Journal of Environmental Sciences 2022年第1期34卷 392-399页

作者： Jialing Ni Jiayuan Ji Yu-You Li Kengo kubota Department of Civil and Environmental Engineering Graduate School of EngineeringTohoku University6-6-06 AobaAramakiAoba-ku 980-8579SendaiJapan Department of Frontier Science for Advanced Environment Graduate School of Environmental StudiesTohoku University6-6-06 AobaAramakiAoba-ku 980-8579SendaiJapan

Two anaerobic membrane bioreactors(AnMBRs)equipped with different membrane pore size(0.4 or 0.05μm)were operated at 25℃and fed with domestic *** hydraulic retention time(hRT)of the reactors was *** microbial communities of the two AnMBRs were investigated by 16S rRNA gene amplicon sequencing to see the effects of *** predominant Archaea was an aceticlastic methanogen *** composition of hydrogenotrophic methanogens changed with the hRTs:the population of Methanobacterium was higher for longer hRTs,whereas the population of unclassified Methanoregulaceae was higher for shorter *** Anaerolineae,Bacteroidia and Clostridia bacteria were dominant in both of the reactors,with a combined relative abundance of over 55%.The relative abundance of Anaerolineae was proportional to the biogas production *** change in the population of hydrogenotrophic methanogens or Anaerolineae can be used as an indicator for process *** sum of the relative abundance of Anaerolineae and Clostridia fluctuated slightly with changes in the hRT in both AnMBRs when the reactor was stably *** co-occurrence analysis revealed the relative abundance of the operational taxonomic units belonging to Anaerolineae and Clostridia was functionally equivalent during the treatment of real domestic sewage.A principal coordination analysis revealed that the changes in the microbial community in each reactor were consistent with the change of *** addition,both the hRT and the stability of the process are important factors for maintaining microbial community structures.

关键词： Anaerobic membrane bioreactor Co-occurrence analysis hydrogenotrophic methanogen hRT Microbial community structure Principal coordinate analysis

Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence in situ hybridization

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Engineering 2022年第2期8卷 61-66页

作者： Kengo kubota Kei Igarashi Masayoshi Yamada Yasuyuki Takemura Yu-You Li hideki harada Department of Civil and Environmental Engineering Tohoku UniversitySendai 980-8579Japan Department of Urban Environmental Design and Engineering National Institute of TechnologyKagoshima CollegeKirishima 899-5193Japan New Industry Creation hatchery Center Tohoku UniversitySendai 980-8579Japan

A microbial community structure of granules harvested from an anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescence in situ hybridization(FISh)and clone library *** of Syntrophorhabdaceae and Cryptanaerobacter were observed to be responsible for phenol *** accurate taxonomic assignment of Cryptanaerobacter clones,phylogenetic analysis using nearly full-length 16S ribosomal RNA(rRNA)gene sequences was *** oligonucleotide probes were designed to detect the following three taxonomic groups:Syntrophorhabdaceae,Cryptanaerobacter,and *** analysis of thin sections of anaerobic granules showed a random distribution of bacteria and ***,a well-defined distribution of Syntrophorhabdaceae,Cryptanaerobacter,and Syntrophus was *** and Syntrophus were found on the outer layer of the granules and were closely associated with each other,while Syntrophorhabdaceae was located in the deeper part of the *** specific distribution of the bacteria is most likely due to their metabolic association and affinity for the *** degradation in the granular sludge was observed to be carried out in the following ***,Cryptanaerobacter converts phenol to benzoate,which is then degraded by Syntrophus into *** syntrophic degradation of phenol occurs near the surface of the granule,where the phenol concen-tration is *** the deeper part of the granule,where the phenol concentration is lower,Syntrophorhabdaceae degrades phenol into *** observed that Syntrophorhabdaceae is less likely to produce benzoate as an intermediate to feed the neighboring organisms,which contradicts the theo-ries presented by previous studies.

关键词： Cryptanaerobacter Fluorescence in situ hybridization Anaerobic phenol degradation Syntrophorhabdaceae Syntrophus UASB granular sludge

Naldemedine在中国健康成年人的安全性、耐受性和药代动力学特征研究

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中国临床药理学杂志 2024年第11期40卷 1633-1637页

作者：杨雅茹郑亮张炜豆红红藤谷航平窪田竜二胡伟安徽医科大学第二附属医院药物临床试验研究中心安徽合肥230601 平安盐野义有限公司上海200032 日本盐野义制药株式会社日本大阪541-0042

目的评价naldemedine单、多次给药在中国健康成人受试者中的安全性、耐受性和药代动力学特征。方法10例中国健康受试者单、多次口服naldemedine后,用液相色谱质谱联用仪检测血浆中naldemedine以及代谢物(nor-naldemedine)的浓度,经Phoen... 详细信息

目的评价naldemedine单、多次给药在中国健康成人受试者中的安全性、耐受性和药代动力学特征。方法10例中国健康受试者单、多次口服naldemedine后,用液相色谱质谱联用仪检测血浆中naldemedine以及代谢物(nor-naldemedine)的浓度,经Phoenix WinNonlin 8.3.1软件计算药代动力学参数。结果相比于单次给药,多次给药后naldemedine的tmax(单次为1.06 h,多次为1.08 h)、C_(max)(单次为4.16 ng·mL^(-1),多次为4.11 ng·mL^(-1))均无明显变化,AUC0-τ高于单次给药(单次为23.88 ng·h·mL^(-1),多次为28.93 ng·h·mL^(-1)),t_(1/2)稍有延长(单次为9.26 h,多次为12.50 h),多次给药后有轻微蓄积(C_(max)的蓄积比为0.99,AUC的蓄积比为1.22);nor-naldemedine的t_(1/2)(单次为18.10 h,多次为29.30 h)、C_(max)(单次为0.19 ng·mL^(-1),多次为0.38 ng·mL^(-1))和AUC0-τ(单次为3.13 ng·h·mL^(-1),多次为6.02 ng·h·mL^(-1))均明显升高,tmax(单次为3.49 h,多次为3.56 h)无明显变化。代谢物与naldemedine的C_(max)比值和AUC比值明显升高,多次给药后存在蓄积(C_(max)的蓄积比为2.01,AUC的蓄积比为1.95)。所有治疗后出现的不良事件均为轻度。结论中国健康受试者连续多次(第1天单次、第4~13天每天1次)口服naldemedine片0.2 mg后,在体内消除较快,有轻度蓄积,在多次给药阶段第2次给药前达到稳态,且有良好的安全性和耐受性。

关键词： naldemedine nor-naldemedine 中国健康受试者药代动力学安全性

喂食中长期缺乏亚硝酸盐和硝酸盐可导致小鼠代谢综合征、内皮功能障碍及心血管病死亡

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中华高血压杂志 2017年第6期25卷 507-507页

作者：刘莉叶鹏 Kina-Tanada M Sakanashi M Tanimoto A Kaname T Matsuzaki T Noguchi K Uchida T Nakasone J Kozuka C Ishida M kubota h Taira Y Totsuka Y Kina SI Sunakawa h Omura J Satoh K Shimokawa h Yanagihara N Maeda S Ohya Y Matsushita M Masuzaki h Arasaki A Tsutsui M

一氧化氮不仅通过一氧化氮合成酶（nitric oxide synthases,NOS）由L-精氨酸合成,还可由其惰性代谢物,亚硝酸盐和硝酸盐合成。绿叶蔬菜中含有丰富的硝酸盐。但是膳食中亚硝酸盐/硝酸盐的缺乏是否导致疾病的发生还有待澄清。该研究人员假... 详细信息

一氧化氮不仅通过一氧化氮合成酶（nitric oxide synthases,NOS）由L-精氨酸合成,还可由其惰性代谢物,亚硝酸盐和硝酸盐合成。绿叶蔬菜中含有丰富的硝酸盐。但是膳食中亚硝酸盐/硝酸盐的缺乏是否导致疾病的发生还有待澄清。该研究人员假设,喂食中长期缺乏亚硝酸盐/硝酸盐会诱导小鼠发生代谢综合征。

关键词：亚硝酸盐和硝酸盐代谢综合征内皮功能障碍心血管病一氧化氮合成酶小鼠喂食死亡

Impact of endothelial nitric oxide synthase activation on accelerated liver regeneration in a rat ALPPS model

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World Journal of Gastroenterology 2023年第5期29卷 867-878页

作者： hitoshi Masuo Akira Shimizu hiroaki Motoyama Koji kubota Tsuyoshi Notake Takahiro Yoshizawa Kiyotaka hosoda Koya Yasukawa Akira Kobayashi Yuji Soejima Division of Gastroenterological Hepato-Biliary-PancreaticTransplantation and Pediatric SurgeryDepartment of SurgeryShinshu University School of MedicineMatsumoto 390-8621Japan

BACKGROUND Although the associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)induces more rapid liver regeneration than portal vein embolization,the mechanism remains *** To assess the influence of inflammatory cytokines and endothelial nitric oxide synthase(eNOS)activation on liver regeneration in *** The future liver remnant/body weight(FLR/BW)ratio,hepatocyte proliferation,inflammatory cytokine expression,and activation of the Akt-eNOS pathway were evaluated in rat ALPPS and portal vein ligation(PVL)*** proliferation was assessed based on Ki-67 expression,which was confirmed using *** serum concentrations of inflammatory cytokines were measured using enzyme linked immune-solvent *** Akt-eNOS pathway was assessed using western *** explore the role of inflammatory cytokines and NO,Kupffer cell inhibitor gadolinium chloride(GdCl3),NOS inhibitor N-nitro-arginine methyl ester(L-NAME),and NO enhancer molsidomine were administered *** The ALPPS group showed significant FLR regeneration(FLR/BW:1.60%±0.08%,Ph that observed in the PVL group(1.33%±0.11%)48 h after *** the ALPPS group,serum interleukin-6 expression was suppressed using GdCl3 to the same extent as that in the PVL ***,the FLR/BW ratio and Ki-67 labeling index were significantly higher in the ALPPS group administered GdCl3(1.72%±0.19%,Phan in the PVL group(1.33%±0.11%and 12.78%±1.55%,respectively).Phospho-Akt Ser473 and phospho-eNOS Ser1177 levels were enhanced in the ALPPS group compared with those in the PVL *** was no difference between the ALPPS group treated with L-NAME and the PVL group in the FLR/BW ratio and Ki-67 labeling *** the PVL group treated with molsidomine,the FLR/BW ratio and Ki-67 labeling index increased to the same level as in the ALPPS *** Early induction of inflammatory cytokines may n

关键词： hepatectomy Nitric oxide Liver regeneration Cytokines NG-Nitroarginine methyl ester Molsidomine

Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica

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Chinese Science Bulletin 2013年第10期58卷 1162-1168页

作者： kubota Ryo LABARRE Paul WEIGL Bernhard h LI Yong hAYDOCK Paul JENKINS Daniel M Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHI 96822USA Diagenetics IncHonoluluHI 96822USA Program for Appropriate Technologies in health(PATh) SeattleWA 98109USA human Nutrition Foodand Animal ScienceUniversity of Hawaii at ManoaHonoluluHI 96822USA Blood Cell Storage Inc(BCSI) SeattleWA 98109USA

We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella *** energy is provided by addition of a small amount(he reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change *** classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld *** or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction *** the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably *** findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries.

关键词：沙门氏菌污染检测设备分子诊断 NINA 核酸扩增成本低反应温度仪表

Edaravone inhibits apoptosis caused by ischemia/reperfusion injury in a porcine hepatectomy model

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World Journal of Gastroenterology 2012年第27期18卷 3520-3526页

作者： Mitsugi Shimoda Yoshimi Iwasaki Toshie Okada Keiichi kubota Second Department of Surgery Dokkyo Medical University880 Kita KobayashiMibuTochigi 321-0293Japan

AIM:To investigate the effect of E3-methyl-1-phenyl-2-pyrazolin-5-one (Edr) on hepatic ischemia-reperfusion (I/R) injury and liver regeneration in a porcine hepatectomy ***:One hour ischemia was induced by occluding the vessels and the bile duct of the right and median lobes.A 40% left hepatectomy was performed after *** animals received Edr (3 mg/kg per hour) intravenously and six control animals received saline just before *** liver volume,hemodynamics,aspartate aminotransferase (AST),alanine aminotransferase,lactate dehydrogenase and lactic acid,were compared between the *** expression of transforming growth factor (TGF1) and toll-like receptor (TRL) mRNA in hepatic tissues was examined using reverse transcription polymerase chain *** was demonstrated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining,***:Serum AST (P = 0.029),and toll like receptor 4 level (P = 0.043) were significantly lower after 3 h in animals receiving *** addition,TUNEL staining in Edr-treated pigs showed significantly fewer hepatocytes undergoing apoptosis compared with control *** 1 mo,all factors were non-significantly different between the two ***:Edr is considered to reduce hepatic injury in the early stage of I/R injury in a porcine model.

关键词：缺血/再灌注损伤细胞凋亡肝再生切除术模型依达拉奉 Toll样受体逆转录聚合酶链反应