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Chinese Science Bulletin 2002年第11期47卷 915-917页

作者： Lin, RC Li, LB kuang, ty Chinese Acad Sci Photosynth Res Ctr Inst Bot Beijing 100093 Peoples R China

The violaxanthin de-epoxidase gene was cloned from rice (Oryza sativa subsp. japonica). The full length of the cDNA is 1887 bp, encoding a 446-amino acids protein with the transit peptide of 98 amino acids. The bacterial expression vector pET-Rvde was constructed and the expression quantity of the exogenous protein increased with the induction time by 0.4 mmol/L IPTG. Its molecular weight was similar with that of the native VDE. Western blotting indicated that the expressed protein has immu-nological reaction with the VDE polyclonal antibody. The absorbance spectrum together with xanthophyll pigments quantification by HPLC demonstrated that the expressed VDE has its enzyme activity, which can de-epoxidate violaxanthin into antheraxanthin and zeaxanthin in vitro.

关键词： Oryza saliva L. violaxanthin de-epoxidase clone ex-pression enzyme activity.

Prediction model for corrosion rate of low-alloy steels under atmospheric conditions using machine learning algorithms

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International Journal of Minerals,Metallurgy and Materials 2024年第2期31卷 337-350页

作者： Jingou kuang Zhilin Long School of Civil Engineering Xiangtan UniversityXiangtan 411105China

This work constructed a machine learning(ML)model to predict the atmospheric corrosion rate of low-alloy steels(LAS).The material properties of LAS,environmental factors,and exposure time were used as the input,while the corrosion rate as the output.6 dif-ferent ML algorithms were used to construct the proposed *** optimization and filtering,the eXtreme gradient boosting(XG-Boost)model exhibited good corrosion rate prediction *** features of material properties were then transformed into atomic and physical features using the proposed property transformation approach,and the dominant descriptors that affected the corrosion rate were filtered using the recursive feature elimination(RFE)as well as XGBoost *** established ML models exhibited better predic-tion performance and generalization ability via property transformation *** addition,the SHapley additive exPlanations(SHAP)method was applied to analyze the relationship between the descriptors and corrosion *** results showed that the property transformation model could effectively help with analyzing the corrosion behavior,thereby significantly improving the generalization ability of corrosion rate prediction models.

关键词： machine learning low-alloy steel atmospheric corrosion prediction corrosion rate feature fusion

Synergistic extraction and separation of thorium from rare earths in chloride media using mixture of Cextrant 230 and Cyanex 923

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Journal of Rare Earths 2024年第4期42卷 759-767页

作者： Hui Wang Shengting kuang Wuping Liao School of Rare Earths University of Science and Technology of ChinaHefei230026China Institute of Material and Chemistry Ganjiang Innovation AcademyChinese Academy of SciencesGanzhou341000China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China

In order to lower the usage of expensive Cyanex 923 and increase the extraction capacity of the system of Cextrant 230,the synergistic extraction of thorium from chloride media by a mixture of Cextrant 230 and Cyanex 923 was *** maximum synergistic enhancement coefficient(R)of 1.53 is obtained at 1:1 molar ratio of Cextrant 230/Cyanex *** syne rgistic extracted species of Th^(4+)is determined as ThCl_(4)·2Cextrant 230·Cyanex *** synergistic extraction of Th^(4+)is an entropy-driven exothermic *** loading capacity of 0.60 mol/L mixed extractant for thorium is about 17.10 g/L(calculated as ThO_(2)),and the loaded thorium in the organic phase can be effectively stripped by distilled *** comparison,rare earth cations are barely extracted under the similar conditions,suggesting that the mixtures can be applied to separate thorium from rare earths.A cascade extraction process was developed based on the synergistic extraction system to separate thorium from the hydrochloric acid leaching of *** content of thorium in the leaching solution decreases obviously from 19.90 mg/L to1.4μg/L by 3 stages of extraction,which is superior to sole Cextrant 230 or Cyanex *** introduction of Cextrant 230 into the extraction system not only lowers the usage of Cyanex 923 but also enhances the selective extraction of thorium at low acidity,implying that the synergistic extraction system can selectively extract thorium more efficiently and economically than the sole systems.

关键词： Synergistic extraction Cextrant 230 Cyanex 923 Thorium Rare earths

Hierarchical Privacy Protection Model in Advanced Metering Infrastructure Based on Cloud and Fog Assistance

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Computers, Materials & Continua 2024年第8期80卷 3193-3219页

作者： Linghong kuang Wenlong Shi Jing Zhang School of Computer Science and Mathematics Fujian University of TechnologyFuzhou350118China Fujian Provincial Key Laboratory of Big Data Mining and Applications Fuzhou350118China

The Advanced Metering Infrastructure(AMI),as a crucial subsystem in the smart grid,is responsible for measuring user electricity consumption and plays a vital role in communication between providers and ***,with the advancement of information and communication technology,new security and privacy challenges have emerged for *** address these challenges and enhance the security and privacy of user data in the smart grid,a Hierarchical Privacy Protection Model in Advanced Metering Infrastructure based on Cloud and Fog Assistance(HPPM-AMICFA)is proposed in this *** proposed model integrates cloud and fog computing with hierarchical threshold encryption,offering a flexible and efficient privacy protection solution that significantly enhances data security in the smart *** methodology involves setting user protection levels by processing missing data and utilizing fuzzy comprehensive analysis to evaluate user importance,thereby assigning appropriate protection ***,a hierarchical threshold encryption algorithm is developed to provide differentiated protection strategies for fog nodes based on user IDs,ensuring secure aggregation and encryption of user *** results demonstrate that HPPM-AMICFA effectively resists various attack strategies while minimizing time costs,thereby safeguarding user data in the smart grid.

关键词： AMI cloud and fog assistance fuzzy comprehensive analysis hierarchical threshold encryption

Monitoring of Cell Membrane Microenvironment Based on DNA Nanodevices

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Chemical Research in Chinese Universities 2024年第2期40卷 255-267页

作者： NAN Hexin CAI Ming kuang Shi NIE Zhou State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha410082P.R.China

The cell membrane is a critical barrier for cellular homeostasis,integral to signaling and intercellular communication,and vital for understanding cellular functions and disease *** its microenvironment is crucial for uncovering the molecular basis of physiological and pathological processes associated with the cell membrane,driving the development of bioanalytical toolkits capable of dynamically monitoring the cell surface *** the continuous advancement of functional nucleic acids and dynamic DNA nanotechnology,DNA nanodevices with controllable nanosized geometry,specific molecular recognition,and selective membrane-localization properties offer a versatile platform for probing the cell membrane *** this review,we summarize the current biosensing and membrane-anchoring mechanisms of DNA nanodevices and highlight their use in studying key cell membrane events,including membrane lipid dynamics,transmembrane transport,receptor dimerization,and signal ***,we discuss the challenges and potential future applications of DNA nanodevices in advancing cell membrane biology research and biomedical applications.

关键词： DNA nanodevice Cell membrane Cellular micro-environment Monitoring

Aldehyde oxidase mediated enantioselective metabolic health risk of dinotefuran

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Science China Chemistry 2024年第6期67卷 2079-2091页

作者： Liwei Xu Xinxin Xu Hua kuang Chuanlai Xu Xiaoling Wu State Key Laboratory of Food Science and Resources Jiangnan UniversityWuxi214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and Technology Jiangnan UniversityWuxi214122China Collaborative Innovation center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi214122China

Chiral pollutants often pose significant differential environmental health *** this study,the biotransformation of chiral dinotefuran(DIN)and its enantioselective metabolic toxicity mechanisms have been systemically ***,reversedphase chromatography-high resolution mass spectrometry was developed to quantify the content of DIN R/S chiral enantiomer with pg level sensitivity,revealing a lower elimination rate constant(K_(e))of S-DIN(0.730 h^(-1))than R-DIN(0.746 h^(-1)).Secondly,the interaction mechanism between DIN metabolism and important endogenous bioactive molecules,such as aldehyde oxidase(AOX)and neurotransmitters,was *** DIN nitro-group was converted into a guanidine group by the reducing site of nearby flavin adenine dinucleotide(FAD)in AOX with the preferred higher affinity of ***,the endogenous tryptophan(Trp)aldehyde metabolic intermediate,5-hydroxyindoleacetaldehyde(5-HIAL),provides a persistent electron donor for DIN reduction via the oxidation-catalyzed site in AOX,resulting in remarkable up-regulation of monoamine neurotransmitters such as serotonin and ***,the higher level of neurotransmitters further mediated dysregulation of oxylipin homeostasis via the serotonergic pathway,where S-DIN exhibited more pronounced liver lipid damage and environmental health risk with the accumulated lipid biomarkers,oxidized triglyceride(OxTG)and oxidized sphingomyelin(OxSM).This study elucidates the AOX-mediated enantioselectivity metabolic pathway of DIN,providing a new analytical method for chiral pollutants and paves the way for their health risk assessments.

关键词： neonicotinoid dinotefuran enantiomer aldehyde oxidase metabolomics health risk

The role of Down syndrome cell adhesion molecule in Down syndrome

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Medical Review 2024年第1期4卷 31-41页

作者： ty Hergenreder Tao Yang Bing Ye Life Sciences Institute and Department of Cell and Developmental Biology University of MichiganAnn ArborMIUSA Life Sciences Institute and Department of Cell and Developmental Biology University of Michigan210 Washtenaw AvenueRoom 5403Ann ArborMI48109USA

Down syndrome(DS)is caused by the presence of an extra copy of the entire or a portion of human chromosome 21(HSA21).This genomic alteration leads to elevated expression of numerous HSA21 genes,resulting in a variety of health issues in individuals with *** the genes located in the DS“critical region”of HSA21,Down syndrome cell adhesion molecule(DSCAM)plays an important role in neuronal *** is a growing body of evidence underscoring DSCAM’s involvement in various DS-related *** review aims to provide a concise overview of the established functions of DSCAM,with a particular focus on its implications in *** delve into the roles that DSCAM plays in DS-associated *** the concluding section of this review,we explore prospective avenues for future research to further unravel DSCAM’s role in DS and opportunities for therapeutic treatments.

关键词： Down syndrome DSCAM neuron axon GABAergic synapse

Synergistic strengthening mechanism of Ca^(2+)-sodium silicate to selective separation of feldspar and quartz

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International Journal of Minerals,Metallurgy and Materials 2024年第9期31卷 1985-1995页

作者： Bo Lin Jingzhong kuang Yiqiang Yang Zheyu Huang Delong Yang Mingming Yu School of Resources and Environmental Engineering Jiangxi University of Science and TechnologyGanzhou 341000China Jiangxi Key Laboratory of Mining Engineering Ganzhou 341000China

Inhibitors are important for flotation separation of quartz and *** this study,a novel combined inhibitor was used to separate quartz and feldspar in near-neutral *** inhibition of the combined inhibitor was assessed by micro-flotation *** a series of detection methods were used to detect differences in the surface properties of feldspars and quartz after flotation reagents and put forward the synergistic strengthening *** outcomes were pointed out that pre-mixing combined inhibitors were more effective than the addition of Ca^(2+)and SS in sequence under the optimal proportion of 1:5.A concentrate from artificial mixed minerals that was characterized by a high quartz grade and a high recovery was acquired,and was found to be 90.70wt% and 83.70%,*** was demonstrated that the combined inhibitor selectively prevented the action of the collector and feldspar from Fourier-transform infrared(FT-IR)and adsorption capacity *** results of X-ray photoelectron spectroscopy(XPS)indicated that Ca^(2+)directly interacts with the surface of quartz to increase the adsorption of *** contrast,the chemistry property of Al on the feldspar surface was altered by combined inhibitor due to Na^(+)and Ca^(2+)taking the place of K^(+),resulting in the composite inhibitor forms a hydrophilic structure,which prevents the adsorption of the collector on the surface of feldspar by interacting with the Al active *** combination of Ca^(2+)and SS synergically strengthens the difference of collecting property between quartz and feldspar by collector,thus achieving the effect of efficient separation.A new strategy for flotation to separate quartz from feldspar in near-neutral pulp was provided.

关键词： feldspar quartz Ca^(2+)-sodium silicate selective adsorption flotation separation

Prediction of discharge in a tidal river using the LSTM-based sequence-to-sequence models

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Acta Oceanologica Sinica 2024年第7期43卷 40-51页

作者： Zhigao Chen Yan Zong Zihao Wu Zhiyu kuang Shengping Wang Key Laboratory of Mine Environmental Monitoring and Improving around Poyang Lake of Ministry of Natural Resources East China University of TechnologyNanchang 330013China School of Surveying and Geoinformation Engineering East China University of TechnologyNanchang 330013China

The complexity of river-tide interaction poses a significant challenge in predicting discharge in tidal *** short-term memory(LSTM)networks excel in processing and predicting crucial events with extended intervals and time delays in time series ***,the sequence-to-sequence(Seq2Seq)model,known for handling temporal relationships,adapting to variable-length sequences,effectively capturing historical information,and accommodating various influencing factors,emerges as a robust and flexible tool in discharge *** this study,we introduce the application of LSTM-based Seq2Seq models for the first time in forecasting the discharge of a tidal reach of the Changjiang River(Yangtze River)*** study focuses on discharge forecasting using three key input characteristics:flow velocity,water level,and discharge,which means the structure of multiple input and single output is *** experiment used the discharge data of the whole year of 2020,of which the first 80%is used as the training set,and the last 20%is used as the test *** means that the data covers different tidal cycles,which helps to test the forecasting effect of different models in different tidal cycles and different *** experimental results indicate that the proposed models demonstrate advantages in long-term,mid-term,and short-term discharge *** Seq2Seq models improved by 6%-60%and 5%-20%of the relative standard deviation compared to the harmonic analysis models and improved back propagation neural network models in discharge prediction,*** addition,the relative accuracy of the Seq2Seq model is 1%to 3%higher than that of the LSTM *** assessment of the prediction errors shows that the Seq2Seq models are insensitive to the forecast lead time and they can capture characteristic values such as maximum flood tide flow and maximum ebb tide flow in the tidal cycle *** indicates the significance of the Seq2Seq models.

关键词： discharge prediction long short-term memory networks sequence-to-sequence(Seq2Seq)model tidal river back propagation neural network Changjiang River(Yangtze River)Estuary

Designing Bioorthogonal Reactions for Biomedical Applications

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Research 2024年第3期2023卷 625-650页

作者： Qingfei Zhang Gaizhen kuang Li Wang Ping Duan Weijian Sun Fangfu Ye Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325001China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China. Department of Obstetrics and Gynecology The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325027China. Department of Gastrointestinal Surgery The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou325027China.

Bioorthogonal reactions are a class of chemical reactions that can be carried out in living organisms without interfering with other reactions, possessing high yield, high selectivity, and high efficiency. Since the first proposal of the conception by Professor Carolyn Bertozzi in 2003, bioorthogonal chemistry has attracted great attention and has been quickly developed. As an important chemical biology tool, bioorthogonal reactions have been applied broadly in biomedicine, including bio-labeling, nucleic acid functionalization, drug discovery, drug activation, synthesis of antibody–drug conjugates, and proteolysis-targeting chimeras. Given this, we summarized the basic knowledge, development history, research status, and prospects of bioorthogonal reactions and their biomedical applications. The main purpose of this paper is to furnish an overview of the intriguing bioorthogonal reactions in a variety of biomedical applications and to provide guidance for the design of novel reactions to enrich bioorthogonal chemistry toolkits.

关键词： orthogonal summarized chemistry