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Zinc phosphate-based nanoparticles as alternatives to zinc oxide in diet of weaned piglets

Journal of Animal Science and Biotechnology 2020年第4期11卷 1147-1162页

作者： Silvia Kociova kristyna Dolezelikova Pavel Horky Sylvie Skalickova Daria Baholet Lucie Bozdechova Eva Vaclavkova Jaroslava Belkova Pavel Nevrkla Jiri Skladanka Tomas Do Ondrej Zitka Yazan Haddad Pavel Kopel Ludek Zurek Vojtech Adam kristyna smerkova Department of Chemistry and Biochemistry Mendel University in BrnoZemedelska 1CZ-61300 BrnoCzech Republic Central European Institute of Technology Brno University of TechnologyPurkynova 123CZ-61200 BrnoCzech Republic Department of Animal Nutrition and Forage Production Mendel University in BrnoZemedelska 1CZ-61300 BrnoCzech Republic Institute of Animal Science Pratelstvi 815CZ-10400 Praha UhrinevesCzech Republic Department of Animal Breeding Mendel University in BrnoZemedelska 1CZ-61300 BrnoCzech Republic Department of Inorganic Chemistry Faculty of SciencePalacky University17.listopadu 12CZ-77146 OlomoucCzech Republic Central European Institute of Technology Center for ZoonosesUniversity of Veterinary and Pharmaceutical SciencesBrnoPalackeho 1946/1CZ-61242 BrnoCzech Republic.

Background: The high doses of zinc oxide(Zn O) administered orally to piglets for the prevention of diarrhea and increase of growth rate can contaminate pig farms and the surrounding environment. Therefore, there is a need to find a replacement of high doses of dietary Zn O with an equally effective alternative. In the present study, the effect of two formulations of zinc phosphate-based nanoparticles(Zn A and Zn C NPs) on growth performance,intestinal microbiota, antioxidant status, and intestinal and liver morphology was evaluated. A total of 100 weaned piglets were randomly divided into 10 equal groups with the base diet(control) or the base diet supplemented with Zn A, Zn C, or Zn O at concentrations 500, 1000, and 2000 mg Zn per kilogram of diet. Supplements were given to animals for 10 days. Fecal samples were collected on day 0, 5, 10 and 20. At the end of the treatment(day 10),three piglets from each group were sacrificed and ***: Comparing to that of control, the significantly higher piglet weight gain was observed in all piglet groups fed with Zn A(P < 0.05). Differences in the total aerobic bacteria and coliform counts in piglet feces after NPs supplementation compared to that of control and Zn O groups were also found(P < 0.05). The majority of aerobic culturable bacteria from the feces represented Escherichia(28.57–47.62%), Enterococcus(3.85–35.71%), and Streptococcus(3.70–42.31%) spp. A total of 542 Escherichia coli isolates were screened for the virulence genes STa,STb, Stx2, F4, and F18. The substantial occurrence of E. coli virulence factors was found on day 5, mainly in fimbrillary antigen and thermostable toxins, except for piglets fed by Zn C. Zn treatment decreased Zn blood levels in piglets fed with Zn O and Zn A(500 mg/kg) and increased in Zn C(2000 mg/kg) compared to that of control(P < 0.05). The antioxidant status of piglets was affected only by Zn A. While some changes in the liver and the intestinal morphology of piglets with

关键词： Antioxidant status Diet E.coli STa STb Stx2 F4 F18 Fecal coliforms Microbiota

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Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

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Journal of Animal Science and Biotechnology 2019年第2期10卷 463-474页

作者： Pavel Horky Sylvie Skalickova Lenka Urbankova Daria Baholet Silvia Kociova Zuzana Bytesnikova Eliska Kabourkova Zuzana Lackova Natalia Cernei Milica Gagic Vedran Milosavljevic Vendula Smolikova Eva Vaclavkova Pavel Nevrkla Pavel Knot Olga Krystofova David Hynek Pavel Kopel Jiri Skladanka Vojtech Adam kristyna smerkova Department of Animal Nutrition and Forage Production Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Department of Chemistry and Biochemistry Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Central European Institute of Technology Brno University of TechnologyPurkynova 123CZ-612 00 BrnoCzech Republic Institute of Animal Science Komenskeho 1239CZ-517 41 Kostelec nad OrliciCzech Republic Department of Animal Breeding Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic.

Background: Development of new nanomaterials that inhibit or kil bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles(NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including ***: Four formulations of zinc nanoparticles(Zn A, Zn B, Zn C, and Zn D) based on phosphates with spherical(Zn A, Zn B)or irregular(Zn C, Zn D) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus(inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), fol owed by Escherichia coli(IC500.8–1.5 mmol/L). In contrast, methicil in resistant S. aureus(IC501.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial Zn O-based NPs and Zn O. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of Zn A, Zn B, Zn C, and Zn D, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of Zn O-N and Zn O, and one group(control) was fed only by basal diet. The significantly higher(P < 0.05) Zn level in liver and kidney of al treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However,the total aerobic and coliform bacterial population in rat feces significantly decreased(P < 0.05) in al zinc groups after 30 d of the treatment. Furthermore, when compared to the Zn O group, Zn A and Zn C nanoparticles reduced coliforms significantly more(P < 0.05).Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.

关键词： Aerobic bacteria Antibiotics Coliforms Nanomaterials Oxidative stress

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