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Effect and mechanism of tacrolimus on melanogenesis on A375 human melanoma cells

Chinese Medical Journal 2014年第16期127卷 2966-2971页

作者： Huang Haiyan Wang Xiaoyan Ding Xiaolan Xu Qianxi Sonia kay Hwang Wang Fang Du Juan Zhang Jianzhong Department of Dermatology Peking University People's HospitalBeijing 100044 China Department of Allergy Beijing Shijitan Hospital Beijing 100038China

Background Topical tacrolimus has been used for vitiligo as a common treatment option for more than ten years while the underlying mechanism is still *** aim of this study was to investigate the direct effects of tacrolimus on the melanogenesis and migration on human A375 melanoma *** expression of c-KIT mRNA and protein of human A375 cells were also *** The cultured A375 human melanoma cells were randomly assigned to control and tacrolimus treatment groups （10,102,103and 104 nmol/l）.The cell proliferation was measured with Cell Counting Kit-8 *** content was measured with NaOH *** migration assay was used to measure cell *** expression of c-KIT mRNA and protein were measured with real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry *** The cell proliferation of the 103 and 104 nmol/l tacrolimus groups were significantly lower （0.666±0.062 and 0.496±0.038） as compared with the control （0.841±0.110,P 〈0.05）.The mean melanin content in all groups treated with different concentration of tacrolimus （10,102,103,104 nmol/l） increased compared with the control group （P 〈0.05）.Dosedependent increase in cell migration were seen in all tacrolimus-treated groups （P 〈0.01）.The expression of c-KIT mRNA level in A375 cells exposed to tacrolimus （103and 104 nmol/l） had significantly increased by 3.03-fold and 3.19-fold respectively compared with the control （P 〈0.05）.Conclusions Although tacrolimus had no effects on cell proliferation on A375 human melanoma cells,it could increase the melanin content and cell *** expression of c-KIT mRNA and protein increased dose-dependently in tacrolimus-treated groups as compared with the *** study demonstrated that tacrolimus could enhance the melanogenesis and cell migration on A375 cells.

关键词： tacrolimus melanocyte c-KIT melanogenesis cell migration

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现代谱分析技术综述

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强度与环境 1987年第Z1期 60-119页

作者： S.M.kay美 S.l.Marple美吴家驹

本文综述了最近20年推出的有关离散时序谱分析的新方法。对每个方法所假设的基本时序模型作的分析,构成本文了解各种谱分析方法之间差别的共同基础。所讨论的方法包括经典的周期图法、经典的 Blackman-Tukey 法、自回归(最大熵)法、滑... 详细信息

本文综述了最近20年推出的有关离散时序谱分析的新方法。对每个方法所假设的基本时序模型作的分析,构成本文了解各种谱分析方法之间差别的共同基础。所讨论的方法包括经典的周期图法、经典的 Blackman-Tukey 法、自回归(最大熵)法、滑动平均法、自回归滑动平均法、最大似然法、Prony 法和 Pisarenko 法。正文中的一览表对所有方法作了简要的评述,包括基本的参考文献和计算每一谱估计的相应公式

关键词：谱—分析谱—述评信号分析

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Janus相关激酶2基因缺失对胰岛β细胞存活及其功能的影响

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中华糖尿病杂志 2017年第10期9卷 612-616页

作者：何晓玉熊飞 kay-Uwe Wagner 王从义华中科技大学同济医学院附属同济医院生物医学研究中心武汉430030 美国内布拉斯加大学医学中心

目的探讨Janus相关激酶2（Jak2）对小鼠胰岛β细胞存活及其功能的影响.方法将诱导型胰岛β细胞特异性Cre转基因小鼠（Rip-CreER tg）与条件性Jak2基因敲除小鼠（Jak2f/f）反复杂交后得到Rip-CreER-Jak2f/f后代,Rip-CreER阳性Jak2双lox... 详细信息

目的探讨Janus相关激酶2（Jak2）对小鼠胰岛β细胞存活及其功能的影响.方法将诱导型胰岛β细胞特异性Cre转基因小鼠（Rip-CreER tg）与条件性Jak2基因敲除小鼠（Jak2f/f）反复杂交后得到Rip-CreER-Jak2f/f后代,Rip-CreER阳性Jak2双loxp的雄性小鼠（Rip-CreER-Jak2f/f）为实验组（KO组,40只）,同窝生的Rip-CreER阴性Jak2f/f雄性小鼠作为对照组（40只）.KO组与对照组小鼠体重20-22 g,鼠龄8周.KO组于8周时腹腔注射他莫昔芬诱导β细胞特异性敲除Jak2基因,对照组给予同样剂量他莫昔芬诱导,在他莫昔芬诱导后一周开始实验检测.采用聚合酶链反应及Western blotting分析检测Jak2在β细胞中的表达缺失,监测小鼠随机血糖及腹腔糖耐量实验评价小鼠胰岛功能.给予KO组和对照组小鼠不同剂量的链脲佐菌素（STZ）刺激,观察Jak2对β细胞存活的调节作用.两两比较采用独立样本的t检验进行统计分析,糖尿病发病率及小鼠病死率用Kaplan-Meier生存模型进行分析.结果成功建立了诱导型胰岛β细胞特异性敲除Jak2小鼠模型.随机血糖监测发现KO组小鼠无自发糖尿病.KO组与对照组小鼠在150 mg/kg的STZ刺激下,糖尿病发生率差异无统计学意义（83.3%比91.6%,P=0.3928）.在亚致病剂量100 mg/kg的STZ刺激下两组小鼠的糖尿病发病率均为20%,2组平均血糖值差异无统计学意义[（11.0 ± 6.6）比（12.0 ± 5.7）mmol/l,t=0.7931,P=0.4258].给予KO组及对照组小鼠致死剂量250 mg/kg的STZ刺激,KO组与对照组小鼠死亡率在给药后第25天无统计学差异（92.3%比93.75%,P=0.5540）.结论小鼠胰岛β细胞的Jak2基因高表达,但对β细胞的存活及功能无影响.

关键词： Jak2基因 β细胞氧化应激胰岛功能

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新南威尔士新生儿囊性纤维化筛查对其肺部结局的影响

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世界核心医学期刊文摘（儿科学分册） 2006年第A3期2卷 60-61页

作者： Mc kay K.O. Waters D.l. Gaskin K.J. 贺文龙 Department of Respiratory Medicine Children's Hospital at Westmead Locked Bag 4001 Westmead NSW 2145 Australia

Objectives: To determine whether early treatment of cystic fibrosis made possible by diagnosis after newborn screening results in improved pulmonary outcomes in adolescence. Study design: Both screening (SG) and non-screening groups (NSG) comprised a cohort of children from Australia previously studied at 1, 5, and 10 years of age. The groups were compared on measures of clinical status obtained during their comprehensive annual review conducted at or near the 15th birthday of the subjects. Results: Data were collected on 48 of 57 original subjects in the NSG (7 had died; 2 were lost to follow-up) and 52 of 60 original subjects in the SG (4 had died; 2 transferred out of the country; 2 were lost to follow-up). Those dying in the SG were significantly older (by 48 months, P lly significant differences were found between the groups in nutritional status. However, subjects in the SG displayed statistically better total Shwachman-Kulczycki scores (7.0, P ≤ .05), chest x-ray scores (2.3, P ≤ .05) and lung function (forced expiratory volume in 1 second by 12.3% , P ≤ .01; forced vital capacity by 12.6% , P ≤ .01; and mean airflow by 23.3% , P ≤ .01). Conclusions: The previously observed advantage conferred by cystic fibrosis newborn screening on clinical outcomes in infancy and childhood is still apparent in adolescence.

关键词：囊性纤维化新南威尔士用力呼气量最大肺活量青少年期失访营养状况有显著性差异试验设计临

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Prevalence and risk factors for impaired renal function among Asian patients with nonalcoholic fatty liver disease

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Hepatobiliary & Pancreatic Diseases International 2024年第3期23卷 241-248页

作者： Chao Sun George Boon-Bee Goh Wan-Cheng Chow Wah-Kheong Chan Grace lai-Hung Wong Wai-kay Seto Yi-Hsiang Huang Han-Chieh lin I-Cheng lee Hye Won lee Seung Up Kim Vincent Wai-Sun Wong Jian-Gao Fan Center for Fatty liver Disease Department of GastroenterologyXinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai Key Lab of Pediatric Gastroenterology and NutritionShanghai 200092China Department of Gastroenterology&Hepatology Singapore General HospitalSingaporeSingapore Gastroenterology and Hepatology Unit Department of MedicineFaculty of MedicineUniversity of MalayaKuala LumpurMalaysia Department of Medicine and Therapeutics and State Key laboratory of Digestive Disease The Chinese University of Hong KongHong KongChina Department of Medicine The University of Hong KongHong KongChina Institute of Clinical Medicine National Yang Ming Chiao Tung University School of MedicineTaipeiTaiwanChina Division of Gastroenterology and Hepatology Taipei Veterans General HospitalTaipeiTaiwanChina Faculty of Medicine National Yang Ming Chiao Tung University School of MedicineTaipeiTaiwanChina Department of Internal Medicine Yonsei University College of MedicineSeoulKorea

Background:Nonalcoholic fatty liver disease(NAFlD)is associated with impaired renal function,and both diseases often occur alongside other metabolic ***,the prevalence and risk factors for impaired renal function in patients with NAFlD remain *** objective of this study was to identify the prevalence and risk factors for renal impairment in NAFlD ***:All adults aged 18-70 years with ultrasound-diagnosed NAFlD and transient elastography examination from eight Asian centers were enrolled in this prospective *** fibrosis and cirrhosis were assessed by FibroScan-aspartate aminotransferase(FAST),Agile 3+and Agile 4 *** renal function and chronic kidney disease(CKD)were defined by an estimated glomerular filtration rate(eGFR)with value ofl/min/1.73 m^(2) andl/min/1.73 m^(2),respectively,as estimated by the CKD-Epidemiology Collaboration(CKD-EPI)***:Among 529 included NAFlD patients,the prevalence rates of impaired renal function and CKD were 37.4%and 4.9%,*** multivariate analysis,a moderate-high risk of advanced liver fibrosis and cirrhosis according to Agile 3+and Agile 4 scores were independent risk factors for CKD(Plasma glucose(FPG)and blood pressure were significantly associated with impaired renal function after controlling for the other components of metabolic syndrome(Plycemia,those with prediabetes[FPG≥5.6 mmol/l or hemoglobin A1c(HbA1c)≥5.7%]were more likely to have impaired renal function(Plusions:Agile 3+and Agile 4 are reliable for identifying NAFlD patients with high risk of *** glycemic control in the prediabetic stage might have a potential renoprotective role in these patients.

关键词： Nonalcoholic fatty liver disease Impaired renal function Agile 3+ Agile 4 Metabolic syndrome

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社交和新闻媒体大数据的多模态分析方法

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当代修辞学 2023年第6期 47-60页

作者：凯·奥哈洛伦高塔姆·帕金闵昊瞿桃(译) 利物浦大学传媒系英国利物浦同济大学外国语学院上海200092

传统的多模态分析首先为语言、图像和其他资源及符号间关系(如图文关系)建构抽象框架,然后用一些实例演示框架的应用。最近,随着大数据分析的多模态转向,多模态研究开始通过大数据分析来测试和验证其理论框架。由于计算模型的开发需要... 详细信息

传统的多模态分析首先为语言、图像和其他资源及符号间关系(如图文关系)建构抽象框架,然后用一些实例演示框架的应用。最近,随着大数据分析的多模态转向,多模态研究开始通过大数据分析来测试和验证其理论框架。由于计算模型的开发需要以多模态理论为基础,并使用大量经过分析的文本进行训练,所以本文提出将多模态框架与现有的大数据、云计算、自然语言处理、图像处理、视频处理和语境元数据计算模型进行融合。这种跨领域的融合能有效地改进计算工具和技术,也能将多模态理论用于理解多模态交际的前沿研究之中。为此,我们探索了如何将现有的计算工具和方法集成为一个多模态分析平台(MAP),用于搜索、存储和分析在线媒体中的文本、图像和视频,使结果可视化。在本文中,我们讨论了用大数据方法进行多模态话语分析的优势以及存在的局限性。我们认为要改进大数据分析的计算技术,需要将有关语言、图像、元数据和其他资源的知识整合为符号系统(而不是简单的符号和像素集)((1))。

关键词：多模态分析新闻媒体社交媒体大数据

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摘叶和修剪对“奇科”(Chico)核桃(Juglans regia l.)花芽分化和形成的影响

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落叶果树 1980年第S1期 17-18+6页

作者： kay Ryugo ,David E.Ramos ,史传铎

在一年的不同时期对旺长幼树的二次枝短截到基部芽上，证明枝端的这些芽形成后四周内即由营养状态转变为生殖状态。在７月份把７个顶节摘叶或摘叶加黄化处埋，不能阻滞这些节位的芽于翌年形成雌花，修剪大大地促进了分化过程。

关键词：摘叶新梢除叶 Chico Juglans regia l 核桃胡桃

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Health Risk Assessment in Children by Arsenic and Mercury Pollution of Groundwater in a Mining Area in Sonora, Mexico

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Journal of Geoscience and Environment Protection 2019年第6期7卷 90-105页

作者： José leopoldo Mendoza-lagunas Diana María Meza-Figueroa Marco Antonio Martínez-Cinco Mary kay O’Rourke Elena Centeno-García Francisco Martin Romero leticia García-Rico María Mercedes Meza-Montenegro Programa de Doctorado en Ciencias en Biotecnologia Instituto Tecnológico de Sonora Ciudad Obregón México División de Ciencias Exactas y Naturales Departamento de Geología Universidad de Sonora Rosales y Encinas Hermosillo México División de Estudios de Posgrado Facultad de Ingeniería Química Universidad Michoacana de San Nicolás de Hidalgo (UMSNH) Morelia México Mel & Zuckerman College of Public Health University of Arizona Tucson AZ USA Instituto de Geología Universidad Nacional Autónoma de México Ciudad Universitaria Delegación Coyoacán Ciudad de México México Centro de Investigación en Alimentos y Desarrollo Hermosillo México Dirección de Recursos Naturales InstitutoTecnológico de Sonora Obregón México

A highly acidic spill from one of the most important copper mines in the Americas (Buenavista del Cobre) occurred in Sonora, Mexico in August, 2014. 40,000 m3 of metals-enriched sulphate solution escaped into the Sonora River representing the worst ecological disaster in Mexican history. The spill affected the economy, water accessibility and the health of residents near the river. Despite the economic importance of mining in this area, no environmental assessment was undertaken. There is no information about the health impact of this event on the local population. The study assesses the health risk among children exposed to arsenic and mercury via groundwater in five communities located in a mining area into the Sonora River Basin, Mexico. local drinking water from the localities was sampled one year after the spill and it was analyzed for arsenic and mercury by Atomic Absorption Spectrometry coupled with Hydride Generation (AAS/HG). Further, some of the traditional local foods were sampled and analyzed for the same elements. Results indicate that levels of arsenic in drinking water did not exceed the Mexican Norm (25 μg As/l) or the EPA Guideline (10 μg As/l). The concentrations of mercury exceeded the WHO and Mexican legislation value (1.0 μg Hg/l). The Hazard Quotient (HQ) values for arsenic were >1 in 42% of children exposed and for mercury in 67% of the children. Foods concentrations contained in the ranges of 9.2 to 62.0 μg As/Kg and 0.28 to 42 μg Hg/Kg for arsenic and mercury respectively. These values are below the Codex Alimentarius limits. Children affected by mining activities are at risk of developing chronic diseases associated with low arsenic and mercury exposure via groundwater consumption, without consideration of the contribution from these metals by other important exposures routes.

关键词： Hazard Quotient Mining Mercury Arsenic Children

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代谢综合征的发育程序化:利用第二代测序技术分析母胎高果糖摄取对子代大鼠转录组的影响（英文）

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生理学报 2016年第5期68卷 557-567页

作者：赵咏梅田佑霖吕史提吴芎历李伟嘉华瑜高雄长庚医院生物医学转化研究所高雄83301 高雄长庚医院儿童肾脏科高雄83301 高雄长庚医院泌尿科高雄83301

临床证据显示高果糖摄取与代谢综合征现象产生有高度相关性,然而关于母体在怀孕与哺乳期高果糖摄取对子代代谢综合征的发育程序化影响的研究却相对缺乏。本研究利用第二代测序(next-generation sequencing)技术分析母鼠高果糖摄取对新... 详细信息

临床证据显示高果糖摄取与代谢综合征现象产生有高度相关性,然而关于母体在怀孕与哺乳期高果糖摄取对子代代谢综合征的发育程序化影响的研究却相对缺乏。本研究利用第二代测序(next-generation sequencing)技术分析母鼠高果糖摄取对新生第一天、离乳后一天(出生后三周)、以及成年期(出生后三个月)子代大鼠脑部、心脏、肾脏与膀胱四个器官转录组的影响。结果显示:(1)母鼠在怀孕与哺乳期高果糖摄取,会程序化胎鼠使其于成年期产生代谢综合征;(2)同时,在仔鼠脑部、心脏、肾脏与膀胱四个器官引起不同程度的转录组变化;(3)在新生第一天子代大鼠,有两个基因,分别是Errfi1与Ctgf,同时在四个器官中表现受到影响,此现象在子代离乳与成年期则消失;(4)参与果糖代谢、糖酵解及糖异生、脂肪代谢、以及胰岛素信号传导通路的基因转录也受到不同程度影响,此现象在新生第一天尤为显著。该结果提示,可利用第二代测序技术寻找不同年龄层子代器官特异性受母体高果糖摄取影响的基因转录变化,进而研发代谢综合征发育程序化在不同器官引起病变的可能机制与治疗策略。

关键词：发育程序化代谢综合征第二代测序转录组

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Analyses of head and thorax in Eupomphini(Meloidae)suggest that complex behaviors are not associated to changes in general shape

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Integrative Zoology 2023年第5期18卷 949-957页

作者： Mario GONZÁlEZ-FERREIRO Mario GARCÍA-PARÍS lucía SAINZ-ESCUDERO E.Karen lÓPEZ-ESTRADA Museo Nacional de Ciencias Naturales(MNCN-CSIC) MadridSpain Real Jardín Botánico(RJB-CSIC) MadridSpain

INTRODUCTION Evolution of behavioral responses is often dependent on the existence of modified structures that enable the development of the behavioral suite(kay 1978;lundrigan 1996;Alfaro et al.2004;Goyens et al.2015;Higham et al.2015;Sharp et al.2018;Schendel et al.2019).Occasionally,structural modifications are so important that a direct link between morphology and behavior can be inferred(Fleagle 1977;Rodman 1979;losos 1990a;Higham et al.2015).

关键词： enable structural behaviors

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