检索结果-南通市图书馆

Poor maternal nutrition inhibits muscle development in ovine offspring

Journal of Animal Science and Biotechnology 2015年第1期6卷 79-89页

作者： Sarah A Reed Joseline S Raja Maria L Hoffman Steven A Zinn Kristen E Govoni Department of Animal Science University of ConnecticutStorrsCT 06269USA

Background： Maternal over and restricted nutrition has negative consequences on the muscle of offspring by reducing muscle fiber number and altering regulators of muscle growth. To determine if over and restricted maternal nutrition affected muscle growth and gene fed 60%, 100% or 140% of National Research Council and protein expression in offspring, 36 pregnant ewes were requirements from d 31 ＋ 1,3 of gestation until parturition. Lambs from control-fed （CON）, restricted-fed （ReS） or over-fed （OVeR） ewes were necropsied within 1 d of birth （n = 18） or maintained on a control diet for 3 mo （n = ] 5）. Semitendinosus muscle was collected for immunohistochemistry, and protein and gene expression analysis. Results： Compared with CON, muscle fiber cross-sectional area （CSA） increased in ReS （58%） and OVeR （47%） lambs at 1 d of age （P〈 0.01）; however at 3 mo, CSA decreased 15% and 17% compared with CON, respectively （P 〈 0.01）. Compared with CON, muscle lipid content was increased in OVeR （212.4%） and ReS （92.5%） at d 1 （P 〈 0.0001）. Muscle lipid content was increased 36.1% in OVeR and decreased 23.6% in ReS compared with CON at 3 mo （P 〈 0.0001）. At d 1, myostatin mRNA abundance in whole muscle tended to be greater in OVeR （P = 0.07） than CON. Follistatin mRNA abundance increased in OVeR （P = 0.04） and tended to increase in ReS （P = 0.06） compared with CON at d 1. However, there was no difference in myostatin or follistatin protein expression （P 〉 0.3）. Phosphorylated Akt （ser473） was increased in ReS at 3 mo compared with CON （P = 0.006）. Conclusions： In conclusion, maternal over and restricted nutrient intake alters muscle lipid content and growth of offspring, possibly through altered gene and protein expression.

关键词： Growth Maternal nutrition Muscle development Sheep

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Culture conditions for equine bone marrow mesenchymal stem cells and expression of key transcription factors during their differentiation into osteoblasts

引用

Journal of Animal Science and Biotechnology 2014年第2期5卷 163-172页

作者： elizabeth R A Glynn Alfredo Sanchez Londono Steven A Zinn Thomas A Hoagl Kristen E Govoni Department of Animal Science University of Connecticut3636 Horsebarn Road Ext.Unit 4040StorrsCT 06269-4040USA Department of environmental and Population Health Tufts University North GraftonMA 01536USA

Background： The use of equine bone marrow mesenchymal stem cells （BMSC） is a novel method to improve fracture healing in horses. However, additional research is needed to identify optimal culture conditions and to determine the mechanisms involved in regulating BMSC differentiation into osteoblasts. The objectives of the experiments were to determine： 1） if autologous or commercial serum is better for proliferation and differentiation of equine BMSC into osteoblasts, and 2） the expression of key transcription factors during the differentiation of equine BMSC into osteoblasts. equine BMSC were isolated from the sterna of 3 horses, treated with purchased fetal bovine serum （FBS） or autologous horse serum （HS）, and cell proliferation determined. To induce osteoblast differentiation, cells were incubated with L-ascorbic acid-2-phosphate and glycerol-2-phosphate in the presence or absence of human bone morphogenetic protein2 （BMP2）, dexamethasone （DeX）, or combination of the two. Alkaline phosphatase （ALP） activity, a marker of osteoblast differentiation, was determined by eLISA. Total RNA was isolated from differentiating BMSC between d 0 to 18 to determine expression of runt-reloted tronscrJption foctor2 （Runx2）, osterix （Osx）, and T-box3 （Tbx3）. Data were analyzed by ANOVA. Results： Relative to control, FBS and HS increased cell number （133 ± 5 and 116 ± 5%, respectively; P 〈 0.001） and 5-bromo- 2＇-deoxyuridine （BrdU） incorporation （167 ± 6 and 120 ± 6%, respectively; P 〈 0.001）. Treatment with DeX increased ALP activity compared with control （1,638 ± 38%; P 〈 0.001）. In the absence and presence of Dex, BMP-2 did not alter ALP activity （P 〉 0.8）. Runt-reloted transcription foctor2 expression increased 3-fold （P 〈 0.001） by d 6 of culture. Osterix expression increased 94old （P 〈 0.05） by d 18 of culture. expression of Tbx3 increased 1.8-fold at d 3 （P 〈 0.01）; however expression was reduced 4-fold at d 18 （P 〈 0.01）. Conclusions： Dexamethasone, but not BMP-2, is required for differentiation of equine BMSC into osteo

关键词： Bone marrow mesenchymal stem cells Cell culture equine Osteoblasts Transcription factors

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular phylogenetic analysis of Leibnitzia Cass.(Asteraceae:Mutisieae:Gerbera-complex),an Asian-North American disjunct genus

引用

Journal of Systematics and evolution 2010年第3期48卷 161-174页

作者： kristen e.BAIRD Vicki A.FUNK Andrea WeeKS Department of environmental Science and Policy George Mason University US National Herbarium Smithsonian InstitutionMRC 166P.O.Box 371012WashingtonDC 20013-7012USA

Leibnitzia comprises six species of perennial herbs that are adapted to high elevation conditions and is one of only two Asteraceae genera known to have an exclusively disjunct distribution spanning central to eastern Asia and North America.Molecular phylogenetic analysis of Leibnitzia and other Gerbera-complex members indicates that Leibnitzia is monophyletic,which is in contrast with our expectation that the American Leibnitzia species L. lyrata and L.occimadrensis would be more closely related to another American member of the Gerbera-complex,namely Chaptalia.Ancestral area reconstructions show that the historical biogeography of the Gerbera-complex mirrors that of the entire Asteraceae,with early diverging lineages located in South America that were followed by transfers to Africa and eurasia and,most recently,to North America.Intercontinental transfer of Leibnitzia appears to have been directed from Asia to North America.Independent calibrations of nuclear(ribosomal DNA internal transcribed spacer region)and chloroplast(trnL-rpl32 intron)DNA sequence data using relaxed clock methods and either mean rate or fossil-based priors unanimously support Miocene and younger divergence times for Gerbera-complex taxa.The ages are not consistent with most Gondwanan vicariance episodes and,thus,the global distribution of Gerbera-complex members must be explained in large part by long-distance dispersal.American species of Leibnitzia are estimated to have diverged from their Asian ancestor during the Quaternary(ca.2 mya)and either migrated overland to North America via Beringia and retreated southwards along high elevation corridors to their present location in southwestern North America or were dispersed long distance.

关键词： Beringia biogeography Gerbera-complex Mutisieae.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Polymorphism of the endothelin-1 gene(rs5370)is a potential contributor to sickle cell disease pathophysiology

引用

Genes & Diseases 2016年第4期3卷 294-298页

作者： kristen G.Navarro Smith e.Agyingi Chinedu K.Nwabuobi Bolaji N.Thomas Department of Biomedical Sciences Rochester Institute of TechnologyRochesterNY14623USA Department of Obstetrics and Gynecology University of South FloridaTampaFLUSA

Sickle cell disease has been shown to demonstrate extensive variability in disease severity among and between individuals,the variability highlighted by differing genetic haplotypes.Despite the abundance of reports of functional significance due to polymorphisms of endothelial nitric oxide synthase(eNOS)and endothelin-1(eT-1)genes,the role of these polymorphisms in mediating sickle cell disease pathophysiology among African Americans is presently unclear.To deconvolute their potential significance among African Americans with sickle cell disease,we examined the genetic diversity and haplotype frequency of eNOS and eT-1 polymorphisms in disease(n Z 331)and control(n Z 379)groups,with a polymerase echain reaction restriction fragment length polymorphism assay.We report that genotypic and allelic frequencies of eNOS variants are not significantly different between groups.eNOS homozygote mutants,which had been shown to have clinical significance elsewhere,showed no statistical significance in our study.On the other hand,and contrary to previous report among Africans with sickle cell disease,the endothelin-1 homozygous mutant variant showed significant difference in genotypic(p Z 2.84e-12)and allelic frequencies(p Z 2.20e-16)between groups.The most common haplotype is the combination of T786C homozygote wildtype variant with homozygote mutant variants of G5665T(eT-1)and Glu298Asp(eNOS).These results show that endothelin-1(rs5370)polymorphism,rather than endothelial nitric oxide synthase polymorphism might play a significant role in disease severity or individual clinical outcomes among African Americans with sickle cell disease.This would have profound implications for designing and/or advancing personalized care for sickle cell patients and relieving disease complications.

关键词： endothelial nitric oxide synthase endothelin-1 Pathophysiology Polymorphisms Sickle cell disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phase 3 trial of first generation protease inhibitor therapy for hepatitis C virus/human immunodeficiency virus coinfection

引用

World Journal of Hepatology 2017年第4期9卷 217-223页

作者： Kenneth e Sherman Minhee Kang Richard Sterling Triin Umbleja kristen Marks Jennifer J Kiser Beverly Alston-Smith Wayne Greaves Adeel A Butt 无 Division of Digestive Diseases University of Cincinnati College of Medicine harvard T.h. Chan School of Public health Department of Gastroenterology Virginia Commonwealth University Division of Infectious Disease Weill Cornell Medical School University of Colorado School of Pharmacy NIAID-Division of AIDS Merck and Co Inc. University of Pittsburgh School of Medicine hamad healthcare Quality Institute the ACTG 5294 BIRTH Study Team

AIM To evaluate efficacy/safety of hepatitis C virus(HCV) protease inhibitor boceprevir with pegylated interferon(PeG-IFN) alfa and weight-based ribavirin(RBV) in a phase 3 trial. MeTHODS A prospective, multicenter, phase 3, open-label, singlearm study of PeG-IFN alfa, weight-based RBV, and boceprevir, with a PeG-IFN/RBV lead-in phase was performed. The HCV/human immunodeficiency virus coinfected study population included treatment na?ve(TN) and treatment experienced(Te) patients. Treatment duration ranged from 28 to 48 wk dependent upon response-guided criteria. All patients had HCV Genotype 1 with a viral load > 10000 IU/ml. Compensated cirrhosis was allowed. Sample size was determined to establish superiority to historical(PeG-IFN plus RBV) rates in sustained viral response(SVR). ReSULTS A total of 257 enrolled participants were analyzed(135 TN and 122 Te). In the TN group, 81.5% were male and 54.1% were black. In the Te group, 76.2% were male and 47.5% were white. Overall SVR12 rates(HCV RNA er limit of quantification, target not detected, target not detected) were 35.6% in TN and 30.3% in Te. Response rates at SVR24 were 28% in TN and 10% in Te, and exceeded those in historical controls. The highest rate was observed in TN non-cirrhotic participants(36.8% and the lowest in Te cirrhotics(26.3%). Cirrhotic TN participants had a 27.8% SVR12 rate and 32.1% of Te non-cirrhotics achieved SVR12. Significantly lower response rates were observed among black participants; in the Te, SVR12 was 39.7% in white participants but only 13.2% of black subjects(P = 0.002). Among the TN, SVR12 was 42.1% among whites and 27.4% among blacks(P = 0.09). CONCLUSION The trial met its hypothesis of improved SVR compared to historical controls but overall SVR rates were low. All-oral HCV treatments will mitigate these difficulties.

关键词：人的免疫不全病毒丙肝病毒 Boceprevir Pegylated 干扰素 A Ribavirin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phylogeography and morphometric variation in the Cinnamon Hummingbird complex:Amazilia rutila(Aves:Trochilidae)

引用

Avian Research 2021年第4期12卷 509-525页

作者： Melisa Vázquez-López Nandadevi Córtes-Rodríguez Sahid M.Robles-Bello Alfredo Bueno-Hernández Luz e.Zamudio-Beltrán kristen Ruegg Blanca e.Hernández-Baños Museo de Zoología Facultad de CienciasUniversidad Nacional Autónoma de MéxicoApartado Postal04510 Mexico CityMexico Department of Biology Ithaca CollegeIthacaNY 14850USA Museo de Zoología Facultad de Estudios Superiores ZaragozaUniversidad Nacional Autónoma de MéxicoMexico CityMexico Department of Biology Colorado State UniversityFort CollinsCO 80523USA

Background:The Mesoamerican dominion is a biogeographic area of great interest due to its complex topography and distinctive climatic history.This area has a large diversity of habitats,including tropical deciduous forests,which house a large number of endemic species.Here,we assess phylogeographic pattern,genetic and morphometric variation in the Cinnamon Hummingbird complex Amazilia rutila,which prefers habitats in this region.This resident species is distributed along the Pacific coast from Sinaloa—including the Tres Marías Islands in Mexico to Costa Rica,and from the coastal plain of the Yucatán Peninsula of Mexico south to Belize.Methods:We obtained genetic data from 85 samples of A.rutila,using 4 different molecular markers(mtDNA:ND2,COI;nDNA:ODC,MUSK)on which we performed analyses of population structure(median-joining network,STRU CTU Re,FST,AMOVA),Bayesian and Maximum Likelihood phylogenetic analyses,and divergence time estimates.In order to evaluate the historic suitability of environmental conditions,we constructed projection models using past scenarios(Pleistocene periods),and conducted Bayesian Skyline Plots(BSP)to visualize changes in population sizes over time.To analyze morphometric variation,we took measurements of 5 morphological traits from 210 study skins.We tested for differences between sexes,differences among geographic groups(defined based on genetic results),and used PCA to examine the variation in multivariate space.Results:Using mtDNA,we recovered four main geographic groups:the Pacific coast,the Tres Marías Islands,the Chiapas region,and the Yucatán Peninsula together with Central America.These same groups were recovered by the phylogenetic results based on the multilocus dataset.Demography based on BSP results showed constant population size over time throughout the A.rutila complex and within each geographic group.ecological niche model projec-tions onto past scenarios revealed no drastic changes in suitable conditions,but revealed some possible refuges.Mor-phometric results showed m

关键词： Amazilia rutila Genetic structure Genetic variation Phylogeography Tres Marías Islands Trochilidae Tropical deciduous forest

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The estrogen metabolite 2-methoxyestradiol regulates eukaryotic initiation factor 4e(eIF4e)and inhibits protein synthesis in MG63 osteosarcoma cells

引用

Genes & Diseases 2016年第2期3卷 153-158页

作者： Avudaiappan Maran kristen L.Shogren Michael J.Yaszemski Department of Orthopedics Mayo ClinicRochesterMN 55905USA

Osteosarcoma is a primary bone tumor that affects children and young adults.The estrogen metabolite 2-methoxyestradiol(2-Me)induces cell death in osteosarcoma cells.To determine whether 2-Me actions involve the control of protein synthesis,we studied the effect of 2-Me on eukaryotic initiation factor 4e(eIF4e)and eIF4e-binding protein 1(4e-BP1)in MG63 osteosarcoma cells.Our results show that 2-Me treatment increases the association of eIF4e with 4e-BP1 in osteosarcoma cells.Also,2-Me decreases the binding of eIF4e protein to 7-methyl-guanosine cap structure,indicating that 2-Me treatment results in the inhibition of translational initiation.These findings are further supported by the inhibition of protein synthesis in 2-Me-treated osteosarcoma cells.Taken together,our studies show that 2-Me-mediated antitumor effects in osteosarcoma cells involve the regulation of protein synthesis,and translational machinery could serve as a target in the treatment of osteosarcoma.

关键词： 2-Methoxyestradiol 4e-BP estrogen metabolite eIF4e Osteosarcoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际农业食品网络和可持续发展委员会

引用

产业与环境 2000年第2期22卷 63-64页

作者： kristen e.Sukalac International Agri-Food Network c/o IFA28 rue Marbeuf75008 ParisFrance

国际农业食品网络(IAFN)是1996年联合国粮农组织世界粮食高峰会议时为协调农业发展领域中与国际组织有关的活动而成立的.该网络只是一个讨论论坛,其中与会者可以确认他们的共同关注和分享各种思路的经验.

关键词：国际农业食品网络可持续发展委员会协会

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SWeeT as Sugar： New Sucrose effluxers in Plants

引用

Molecular Plant 2012年第4期5卷 766-768页

作者： R. Frank Baker kristen A. Leach David M. Braun Division of Biological Sciences Interdisciplinary Plant Group Missouri Maize Center University of Missouri 110 Tucker Hall Columbia MO 65211

Photosynthesis leads to the fixation of carbon in leaves and to the accumulation of carbohydrates （e.g., sucrose, starch）. This assimilated carbon must be transported from the leaves （source tissues） to non-photosynthetic organs （sink tissues）, such as roots and seeds. Despite the essentiality of this process in plants, a complete understanding of the actors involved in sucrose export from leaves is lacking. SWeeT proteins are a newly identified class of sugar transporters that facilitate dif- fusion of sugars across cell membranes down a concentration gradient. In this perspective, we highlight the recent publica- tion of Chen et al. （2012）, which reports that SWeeT proteins transport sucrose across cell membranes, are expressed in a subset of phloem parenchyma cells,

关键词： Candy foliage sugar protein sweeteners plants Fueling actor Carbon Fixation Saccharose snow goose

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

effects of maternal nutrition on the expression of genomic imprinted genes inovine fetuses

Effects of maternal nutrition on the expression of genomic i...

引用

第十四届中国实验动物科学年会

作者： Jingyue (ellie) Duan 张名媛 Kaleigh Flock Sahar Al Seesi Ion Mandoiu Amanda Jones elizabeth Johnson Sambhu Pillai Maria Hoffman Katelyn McFadden Hesheng Jiang Sarah Reed kristen govoni Steve Zinn Zongliang Jiang Xiuchun (Cindy) Tian Department of Animal Science University of Connecticut College of Animal Science and Technology Guangxi University Laboratory Animal Center Guangxi Medical University Department of Computer Science University of Connecticut School of Animal Science Louisiana StateUniversity Agricultural Center

Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parentalorigin. To date, 263 imprinted genes have been identified among all investigated mammalian species.However, only 21 have been described in sheep, of which 11 are annotated in the current ovinegenome. Here, we aimtoi) use DNA/RNA high throughput sequencing to identify new monoallelicallyexpressed and imprinted genes in day 135 ovine fetuses and ii) determine whether maternal diet(100%, 60%, or 140% of National Research Council Total Digestible Nutrients) influences expression ofimprinted genes. We also reported strategies to solve technical challenges in the data analysispipeline. We identified 80 monoallelically expressed, 13 new putative imprinted genes, and fiveknown imprinted genes in sheep using the 263 genes stated above as a guide. Sanger sequencingconfirmed allelic expression of seven genes, CASD1, COPG2, DIRAS3, INPP5 F, PLAGL1, PPP1 R9 A, and SLC22 A18. Among the 13 putative imprinted genes, five were localized in the known sheep imprintingdomains of MeST on chromosome 4, DLK1/GTL2 on chromosome 18 and KCNQ1 on chromosome 21,and three were in a novel sheep imprinted cluster on chromosome 4, known in other species asPeG10/SGCe. The expression of DIRAS3, IGF2, PHLDA2, and SLC22 A18 was altered by maternal diet,albeit without allelic expression reversal. Together, our results expanded the list of sheep imprintedgenes to 34 and demonstrated that while the expression levels of four imprinted genes were changedby maternal diet, the allelic expression patterns were un-changed for all imprinted genes studied.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：