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Copper Delivery by the Copper Chaperone for Chloroplast and Cytosolic Copper/Zinc- Superoxide Dismutases： Regulation and Unexpected Phenotypes in an Arabidopsis Mutant

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Molecular Plant 2009年第6期2卷 1336-1350页

作者： Christopher M. Cohu Salah E. Abdel-Ghany k.thryn A. Gogolin Reynolds Alexander M. Onofrio Jared R. Bodeck.r Jeffrey A. k.mbrel krishna k. niyogi Marinus Pilon Biology Department Colorado State University Fort Collins CO 80523-1878 USA Department of Plant and Microbial Biology University of California Berkeley CA 94720-3102 USA

Copper （Cu） is an important mineral nutrient found in chloroplasts as a cofactor associated with plastocyanin and Cu/Zn superoxide dismutase （Cu/ZnSOD）. Superoxide dismutases are metallo-enzymes found in most oxygenic organisms with proposed roles in reducing oxidative stress. Several recent studies in Arabidopsis have shown that microRNAs and a SQUAMOSA promoter binding protein-lik.7 （SPL7） transcription factor function to down-regulate the expression of many Cu-proteins, including Cu/ZnSOD in both plastids and the cytosol, during growth on low Cu. Plants contain the Cu Chaperone for SOD （CCS） that delivers Cu to Cu/ZnSODs, and, in Arabidopsis, both cytosolic and plastidic CCS versions are encoded by one gene. In this study, we demonstrate that Arabiclopsis CCS transcript levels are regulated by Cu, mediated by microRNA 398 that was not previously predicted to target CCS. The microRNA target site is conserved in CCS of Oryza sativa. The data suggest that Cu-regulated microRNAs may have more mRNA targets than was previously predicted. A CCS null mutant has no measurable SOD activity in the chloroplast and cytosol, indicating an absolute requirement for CCS. When the CCS null mutant was grown on high Cu media, it lack.d both Fe superoxide dismutase （FeSOD） and Cu/ZnSOD activity. However, this did not lead to a visual phenotype and no photosynthetic deficiencies were detected, even after high light stress. These results indicate that Cu/ZnSOD is not a pivotal component of the photosynthetic anti-oxidant system during growth in laboratory conditions.

关键词： Chloroplast photosynthesis copper homeostasis superoxide dismutase microRNA.

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