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Polyfunctional T Cell and Neutralizing Antibody Responses to ACAM2000^TM Smallpox Vaccine Immunization in Primary-Vaccinated Individuals

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Advances in Microbiology 2016年第3期6卷 169-177页

作者： Suchada Sukhumvittaya Silawun Ampol kovit pattanapanyasat Wannee Kantakamalakul Department of Microbiology Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand Center of Excellence for Flow Cytometry Office for Research and Development Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand

Smallpox eradication was successful via prophylactic administration of live attenuated vaccinia virus. As a result of the discontinuation of the smallpox immunization program, many individuals are now susceptible to smallpox virus infection should it be used as a biological weapon. Presently, only individuals at high risk for exposure are required to receive smallpox vaccine, such as laboratory personnel that handle variola/vaccinia virus. This study endeavored to investigate a one-year period of vaccinia virus-specific T cell responses using polychromatic flow cytometry and neutralizing (Nt) antibody responses using plaque reduction neutralization test (PRNT) in individuals receiving primary immunization (n = 5) with ACAM2000TM smallpox vaccine. Functional and phenotypic profiles of vaccinia virus-specific T cell responses were characterized. Each single functional measurement {CD107a/b expression, production of interferon g (IFN-g), macrophage inflammatory protein 1b (MIP-1b), interleukin 2 (IL-2), and tumor necrosis factor a (TNF-a)} demonstrated that vaccinia virus-specific CD8+ T cells were functional at least one time point after vaccination (p ≤ 0.05). However, vaccinia virus-specific CD4+ T cells were functional only for MIP-1b production (p ≤ 0.05). Vaccinia virus-specific CD8+ T cells induced in these individuals showed increased polyfunctionality in at least 2 phenotypes relative to pre-vaccination (p ≤ 0.05). Although only three of five individuals (60%) showed positive Nt antibody (titer ≥ 20) at first month after vaccination, all five individuals (100%) demonstrated Nt antibody at 2 months, post-immunization. Interestingly, all vaccinees could retain the Nt antibody for 6 months after primary vaccination. In conclusion, ACAM2000TM smallpox vaccine induced both polyfunctional T cell-and Nt antibody-responses in primary immunized individuals.

关键词： Smallpox Vaccine Primary Immunization T Cell Neutralizing Antibody

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Green tea extract and epigallocatechin 3-gallate reduced labile iron pool and protected oxidative stress in iron-loaded cultured hepatocytes

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1140-1150页

作者： Somdet Srichairatanakool Kanokwan Kulprachakarn Kanjana Pangjit kovit pattanapanyasat Suthat Fuchaeron Department of Biochemistry Faculty of Medicine Chiang Mai University Chiang Mai Thailand Office of Research and Development Faculty of Medicine Siriraj Hospital Mahidol University Thailand Thalassemia Research Center Institute of Molecular Bioscience Mahidol University Salaya Campus Nakornpathom Thailand

Cellular and mitochondrial damage can be caused by labile iron pool (LIP) and mediated by reactive oxygen species (ROS). Livers of the thalassemias have highly increased levels of LIP and ROS. Green tea extract (GTE) and epigallocatechin 3-gallatte (EGCG) can potentially protect liver inflammation, fibrosis and cancer due to their anti-oxidative and iron-chelating activities. We studied the effects of GTE and EGCG on intracellular LIP and ROS, and mitochondrial membrane potential (ΔΨm) in mouse hepatocyte and HepG2 cell cultures using specific fluorescent techniques. Treatment with GTE (12.5 - 25 mg/dl) and EGCG (25 - 50 μM) significantly lowered levels of ΔΨm in the mouse hepatocytes;however, combined treatment of 25 μM DFP with GTE and EGCG did not enhance the decrease of hepatic ΔΨm. The results showed that GTE and EGCG effectively removed the intracellular LIP and ROS, and relieved the mitochondria membrane collapse of the liver cells, suggesting a hepatoprotective effect of green tea extract and EGCG in the hepatocytes with iron overload. Their actions might be related to iron-chelating and free radical-scavenging capacities. Whether the effects can improve iron overload and oxidative stress in thalassemia patients remains to be seen upon further examination.

关键词： Green Tea Epigallocatechin Gallate Hepatocytes Labile Iron Pool Iron Reactive OxygenSpecies

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