检索结果-南通市图书馆

Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms

引用

Advances in Bioscience and Biotechnology 2011年第4期2卷 304-310页

作者： Hirofumi Taki Tomoharu Gomi Bryan Knuckley Paul R. Thompson Oliver Vugrek Kazuya Hirata Tatsurou Miyahara kouichiro shinoda Hiroyuki Hounoki Eiji Sugiyama Isao Usui Masaharu Urakaze Kazuyuki Tobe Tetsuya Ishimoto Ran Inoue Ayumi Tanaka Hiroki Mano Hirofumi Ogawa Hisashi Mori 不详

The murine peptidylarginine deiminase (PAD) has five isoforms encoded by different genes and participates in a variety of cellular functions through the citrullination of target proteins. The crystal structure of human PAD4 with a dimeric form was previously solved because of the enzyme’s relevance to rheumatoid arthritis. PAD6, abundant in mouse oocytes and eggs, is believed to take part in early events of embryogenesis, but its biochemical properties are little understood. Here we have purified and characterized a recombinant PAD6. A PAD6 cDNA was cloned from mouse ovary RNA and expressed in Escherichia coli through pET29 and pGEX vectors. When benzoyl-L-arginine ethyl ester was used as a substrate, no appreciable activity was detected with a cell homogenate under conditions where a human PAD4 cDNA caused significant activity. Both proteins were affinity-purified to near homogeneity. The circular dichroism spectra of PAD6 and human PAD4 were similar in the far ultraviolet region. On molecular sieving, PAD6 was eluted faster than human PAD4. The cross-linking of PAD6 with dimethyl suberimidate clearly showed six bands on an sodium dodecyl sulfate-polyacrylamide gel. These results indicate that PAD6 can constitute a hexameric structure. The purified PAD6 still showed no enzymatic activity. This unique structure and loss in enzymatic activity is strongly suggested to favor the formation of egg cytoplasmic sheets as the architectural protein.

关键词： Peptidylarginine deiminase Isoform Dimer Hexamer Mouse Oocytes Cytoplasmic sheets.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BEOL工艺统合Integration——low-k Dual/Damascene的形成

引用

半导体技术 2003年第3期28卷 77-78,73页

作者： LI-Hung Chen Takashi Hayakawa Kaoru Maekawa kouichiro Inazawa Tokyo Electron ShanghaiLtd Tokyo Electron ShanghaiLtdTokyo Electron LtdTokyo Electron Shang haiLtd Japan.

1简介当前,从成膜方法来讲,ILD loW-k材料大致上分为两类:一类是以SiCOH为代表的CVD(化学气相淀积)膜,另一类是SOD(旋转涂层)膜,它包括有机的、无机的和SiCOH.

关键词： BEOL工艺 Dual/Damascene LOW-K材料 SOD沉淀蚀刻技术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Successful Two-Stage Surgical Treatment for Lung Cancer in a Patient with Contralateral Giant Emphysematous Bullae

引用

Open Journal of Thoracic Surgery 2012年第4期2卷 133-135页

作者： Yuji Hirami Katsuhiko Shimizu Riki Okita Shinsuke Saisho Takuro Yukawa Ai Maeda kouichiro Yasuda Masao Nakata Department of General Thoracic Surgery Kawasaki Medical School Okayama Japan Okita Department of General Thoracic Surgery Kawasaki Medical School Okayama Japan

A 67-year-old man was referred for further evaluation of an abnormal chest roentgenogram. Computed tomography showed a 40 × 30 mm mass in the left upper lobe. A giant bulla occupying about two-thirds of the right thorax was found compressing the adjacent lung parenchyma, shifting the mediastinum to the left. The mass was a primary lung cancer, clinical T2aN0M0, stage IB. Preoperative respiratory function evaluation showed poor pulmonary function, with a forced expiratory volume in 1 second of 1070 ml (29.2% of predicted). Therefore, we first performed giant bullectomy by video-assisted thoracoscopic surgery. At 1 month after this operation, improvement of the forced expiratory volume in 1 second significantly to 2140 ml (80.1% of predicted) was observed. Therefore, we performed resection for the tumor. He was discharged after an uneventful postoperative course, and has remained in good condition for 6 months after the operation.

关键词： Giant Emphysematous Bulla Pulmonary Function Bullectmy Lung-Cancer Surgery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

关于多孔MSQ Dual Damascene蚀刻/灰化工艺——为更低损伤和更低k值的多方面损伤评价和化学性质优化

引用

电子工业专用设备 2003年第3期32卷 67-72页

作者： Shin Okamoto CHEN Li-hung Takashi Hayakawa kouichiro Inazawa Tokyo Electron AT Ltd Tokyo Electron AT LtdToko Electron LtdTokyo Electron AT Ltd Yamanashi407-8511JapanYamanashi 407-8511JapanTokyo 107JapanYamanashi 407-8511Japan

对铜(Cu)互连系统使用低k值材料时,应解决的许多相关问题不仅有技术问题,而且有所有权成本(CoO)?质量合格(CoC)问题。从蚀刻和灰化(去除)工艺的角度报道了几种问题的解决方法。对蚀刻工艺,将在此推荐Via1st的Duo248TM方法(Duo248TM是Hon... 详细信息

对铜(Cu)互连系统使用低k值材料时,应解决的许多相关问题不仅有技术问题,而且有所有权成本(CoO)?质量合格(CoC)问题。从蚀刻和灰化(去除)工艺的角度报道了几种问题的解决方法。对蚀刻工艺,将在此推荐Via1st的Duo248TM方法(Duo248TM是Honeywell公司的材料,包含US专利#6,268,457)。对于灰化工艺,建议混合灰化工艺(HybridAshing),即蚀刻和灰化工艺在同一反应室连续进行。这些方法的目标将是65nm以下工艺。

关键词： DualDamascene 低k值 Duo 混合灰化工艺 SCCM

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Headache at the onset of stroke: Frequencies, background characteristics and correlation with mortality

引用

Health 2013年第1期5卷 89-95页

作者： Kazuo Shigematsu Hiromi Nakano Yoshiyuki Watanabe Tatsuyuki Sekimoto kouichiro Shimizu Akihiko Nishizawa Atsushi Okumura Masahiro Makino Touru Seki Kazuhiko Bando Yasushi Kitagawa Department of Epidemiology for Community Health and Medicine Kyoto Prefectural University of Medicine Graduate School of Medical Science Kyoto Japan Department of Internal Medicine The Bando Clinic Kyoto Japan Department of Internal Medicine The Kitagawa Clinic Kyoto Japan Department of Internal Medicine The Nishizawa Clinic Kyoto Japan Department of Internal Medicine The Seki Clinic Kyoto Japan Department of Neurology Japanese Red Cross Kyoto Daini Hospital Kyoto Japan Department of Neurology National Hospital Organization Minami Kyoto Hospital Kyoto Japan Department of Neurosurgery Jujyo Rehabilitation Hospital Kyoto Japan Department of Neurosurgery Kyoto Fushimi Shimizu Hospital Kyoto Japan Department of Neurosurgery Kyoto Kidugawa Hospital Kyoto Japan Department of Neurosurgery Kyoto Prefectural Yosanoumi Hospital Kyoto Japan

Background: Headache is a common symptom and sometimes makes patients worry about stroke. Some stroke patients actually have headache. However, the frequency of headache at the onset of stroke and the relationship between headache and outcomes are not fully clarified. Objective: The aim of the study is to clarify frequency of headache, and to compare patients with headache with patients without headache in their characteristics, risk factors and early mortalities. Methods: We confirmed the presence or absence of onset headache in 1671 patients in the Kyoto Stroke Registry. We studied frequencies of headache, age, sex, blood pressure, arrhythmia, diabetes mellitus, hyperlipemia, tobacco and alcohol use and mortality of stroke patients. Results: Headache was observed in 21.4% of stroke patients;12.0% in cerebral infarction (CI), 29.8% in cerebral hemorrhage (CH), and 93.9% in subarachnoid hemorrhage (SAH). Blood pressure at the first medical examination after stroke was higher in patients with headache. Factors associated with headache were age, diastolic blood pressure, hypertension and hyperlipemia histories, alcohol use, and paresis in CI, age, alcohol and tobacco use and paresis in CH and systolic blood pressure in SAH. The mortality rate 30 days after the stroke event was higher by 6.8% in CI and by 11.4% in CH in patients with headache than that in patients without headache. The hazard ratios for death between patients with headache and patients without headache were 4.94 (1.73 - 14.08, p = 0.003) in CI, and 3.20 (1.10 - 9.36, p = 0.033) in CH. Conclusions: Headache is common in stroke and predicts outcome. Headache is associated with younger age and alcohol use in CI and CH. Patients who could express presence of headache at the onset of CI or CH have higher mortality than patients who could express absence of headache. Headache is important both as an identifier of stroke and as a poor prognostic indicator in stroke.

关键词： Headache Stroke Outcomes Mortality Epidemiology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of a Muon Rotating Target for J-PARC/MUSE

Development of a Muon Rotating Target for J-PARC/MUSE

引用

第18届国际真空大会

作者： Shunsuke Makimura Yasuo Kobayashi Yasuhiro Miyake Naritoshi Kawamura Patrick Strasser Akihiro Koda kouichiro Shimomura Hiroshi Fujimori Kusuo Nishiyama Mineo Kato Kenji Kojima Ryosuke Kadono Muon Science Laboratory High Energy Accelerator Research Organization （KEK）

At the J-PARC muon science facility （J-PARC/MUSE）, a graphite target with 20-mm thickness has been used in vacuum to obtain an intense pulsed muon beam from the RCS 3-GeV proton beam [1]. In the current design, the target frame is constructed using copper with a stainless steel tube embedded for water cooling. The energy deposited by the proton beam at 1MW is evaluated to be 3.3 kW on the graphite target and 600 W on the copper frame by a Monte-Carlo simulation code, PHITS [2]. Graphite materials are known to lose their crystal structure and can be shrunk by the intense proton beam irradiation. Consequently, the lifetime of the muon target is essentially determined by the radiation damage in graphite, and is evaluated to be half a year [3]. Hence, we are planning to distribute the radiation damage by rotating a graphite wheel. Although the lifetime of graphite in this case will be more than 10 years, the design of the bearing must be carefully considered. Because the bearing in J-PARC/MUSE is utilized in vacuum, under high radiation, and in high temperature, an inorganic and solid lubricant must be applied on the bearing. Simultaneously, the temperature at the bearing must be also decreased to extend the lifetime. In 2009, a mock-up of the Muon Rotating Target, which could heat up and rotate a graphite wheel, was fabricated and then several tests were started to select the lubricant, and to decide the structure of the Muon Rotating Target, the control system and so on. In this presentation, the present status of the Muon Rotating Target for J-PARC/MUSE, especially the development of a rotation system in vacuum, will be described.

关键词： J-PARC Muon Target Bearing

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：