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Reduced port surgery for appendectomy:Early experience and surgical technique

World Journal of Surgical Procedures 2013年第2期3卷 8-12页

作者： Shinichiro Mori Kenji Baba Shigehiro Yanagita Yoshiaki Kita kosei maemura Yuko Mataki Yasuto Uchikado Hiroshi Okumura Tetsuhiro Nakajyo Shoji Natsugoe Sonshin Takao Kuniaki Aridome Department of Digestive Surgery Breast and Thyroid Surgery Graduate School of Medicine Kagoshima University Kagoshima 890-8520 Japan Frontier Science Research Center Kagoshima University Kagoshima 890-8520 Japan Department of Surgery Nanpuh Hospital NKagoshima 892-8512 Japan

AIM: To evaluate our experience and surgical technique of laparoscopic appendectomy via reduced port surgery(LARPS). METHODS: Sixteen patients(8 men and 8 women; median age: 31.0 years) who underwent LARPS between November 2009 and May 2012 were included in the present study. We performed LARPS, in which access devices were inserted through an umbilical skin incision with 1 additional skin incision in the left lower abdomen. After setting access devices, pneumoperitoneum was maintained at 10 mmH g using CO2 and a 3 mm trocar was positioned(or direct puncture was performed by the Endo Relief system) under laparoscopic guidance. The mesoappendix was dissected using an ultrasonically activated device. After mesoappendix dissection, ligation was performed near the appendix base and the appendix was dissected using an ultrasonically activated device. The appendix was then removed. At the end of surgery, we administered local anesthesia with ropivacaine 1%(10 mL) for the skin incisions. The outcomes were evaluated in terms of operation time, intraoperative blood loss, length of postoperative hospital stay and surgical complications. RESULTS: Our surgical procedure allowed operators to use instruments as in conventional laparoscopic appendectomy. The basic principle of triangulation of instrumentation was maintained to some degree. LARPS was performed in 9 patients with catarrhal appendicitis, 5 with phlegmonous appendicitis, and 2 with gangrenous appendicitis. The median surgery time was 60 min and the median intraoperative blood loss was 1.2 mL. The median length of postoperative hospitalization was 4 d. There were no conversions to open surgery, no operation-related complications or mortality. CONCLUSION: Our experience and surgical technique suggest that LARPS is a safe and feasible procedure for patients with appendicitis.

关键词： Reduced port surgery Appendectomy Laparoscopy Cosmesis Single incision

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Recording the direct surgeon’s view with an operating microscopic view improves microscopic ophthalmic surgery training

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International Journal of Ophthalmology(English edition) 2023年第9期16卷 1555-1558页

作者： Masayuki Akimoto kosei Tomita Miyo Yoshida Yuki Hama Ophthalmology Osaka Red Cross HospitalTennoji-kuOsaka 543-8555Japan

AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to facilitate the reproduction of a skilled surgeon’s technique by a surgeon in ***:A small camera was attached to the operating microscope with a custom *** surgeon’s view and direct surgeon’s view through this new camera were recorded in the surgical recording *** movies were synchronized and analyzed how do surgeons handle the ***:A small camera attached to the operating microscope allowed the surgeon’s hands motion to be recorded without interfering with the surgeon’s *** surgeons used different methods to manipulate the ultrasound handpiece and the irrigation/aspiration *** in the simple paracentesis procedure,different surgeons used different ***-in-training were able to identify and improve their weaknesses by watching synchronized movies of their hand motions and microscopic ***:Simultaneous recording the surgical field out of the operating microscopic view by a small camera set on the microscope is comprehensive and improves surgeons-in-training understanding and learning surgeries.

关键词： operating microscopic view direct surgeon’s view video recording microscopic surgery training

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Kawasaki disease without changes in inflammatory biomarkers:A case report

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World Journal of Clinical Cases 2022年第35期10卷 13038-13043页

作者： kosei Yamashita Takeru Kanazawa Yoshifusa Abe Takuya Naruto Masaaki Mori Children’s Medical Center Showa University Koto Toyosu HospitalTokyo 135-8577Japan Lifetime Clinical Immunology Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityTokyo 113-8510Japan Department of Rheumatology Clinical Immunology and AllergologySt.Marianna University School of MedicineKanagawa 216-8511Japan

BACKGROUND Kawasaki disease(KD)is diagnosed based on clinical *** tests and other tests are auxiliary diagnostic *** KD is a disease caused by arterial inflammation,many patients with KD have elevated levels of inflammatory biomarkers,such as C-reactive protein(CRP),erythrocyte sedimentation rate(ESR),and serum amyloid A protein(SAA)in blood *** report our experience of a patient with KD who did not have elevated levels of inflammatory *** SUMMARY A 1-year-old boy presented with a 3-day history of *** of the six symptoms of KD were observed,except for changes in the lips and oral *** tests revealed no elevation in CRP,ESR,or SAA *** the blood test results were atypical,the patient was diagnosed with KD based on clinical symptoms and was admitted to the hospital for *** patient was administered intravenous immunoglobulin(IVIG)and *** commencing treatment,the fever persisted;therefore,additional IVIG was administered,the dosage of aspirin was increased,and ulinastatin was *** doses of IVIG were administered and the fever resolved on day 11 of KD symptoms *** tests performed during hospitalization showed normal levels of inflammatory *** examined leucine-rich alpha-2-glycoprotein 1-a protein that is elevated during the acute phase of *** protein levels did not increase during *** This case suggests the need to identify criteria and biomarkers for detecting KD conditions that do not require KD treatment.

关键词： Blood test C-reactive protein Erythrocyte sedimentation rate Intravenous immunoglobulin Kawasaki disease Case report

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Direct imaging of the near field and dynamics of surface plasmon resonance on gold nanostructures using photoemission electron microscopy

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Light(Science & Applications) 2013年第1期2卷 1-8页

作者： Quan Sun kosei Ueno Han Yu Atsushi Kubo Yasutaka Matsuo Hiroaki Misawa Research Institute for Electronic Science Hokkaido UniversitySapporo 001-0021Japan Creative Research Institution Hokkaido UniversitySapporo 001-0021Japan PRESTO Japan Science and Technology AgencyKawaguchi 332-0012Japan Institute of Physics University of TsukubaTsukuba 305-8571Japan

Localized surface plasmon resonance(LSPR)can be supported by metallic nanoparticles and engineered *** understanding of the spatially resolved near-field properties and dynamics of LSPR is important,but remains experimentally *** report experimental studies toward this aim using photoemission electron microscopy(PEEM)with high spatial resolution of sub-10 *** engineered gold nanostructure arrays(such as rods,nanodisk-like particles and dimers)are investigated via PEEM using near-infrared(NIR)femtosecond laser pulses as the excitation *** the LSPR wavelengths overlap the spectrum of the femtosecond pulses,the LSPR is efficiently excited and promotes multiphoton photoemission,which is correlated with the local intensity of the metallic nanoparticles in the near ***,the local field distribution of the LSPR on different Au nanostructures can be directly explored and discussed using the PEEM *** addition,the dynamics of the LSPR is studied by combining interferometric time-resolved pump-probe technique and *** information on the oscillation and dephasing of the LSPR field can be *** results identify PEEM as a powerful tool for accessing the near-field mapping and dynamic properties of plasmonic nanostructures.

关键词： femtosecond laser local field enhancement near-field imaging photoemission electron microscopy surface plasmon resonance

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Isolation, Identification and Tyrosinase Inhibitory Activities of the Extractives from Allamanda cathartica

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Natural Resources 2011年第3期2卷 167-172页

作者： kosei Yamauchi Tohru Mitsunaga Irmanida Batubara 不详

Tyrosinase inhibitory activity of the extractives from A. cathartica was examined and their new bioactivity and potent active compounds were identified. Five compounds, glabridin, new lignan, kaempferol, naringenin, and allamandicin, were isolated by a series of chromatography, and identified by NMR and LC-MS. Among them, glabridin had the high-est tyrosinase inhibitory activity (IC50:2.93 μM) which is 15 times stronger than that of kojic acid used as positive con-trol (IC50:43.7 μM). Moreover the lignan was indentified as 1-[3-(4-allyl-2,6-dimethoxyphenoxy)-4-methoxyphenyl] propane-1,2,diol which was a novel lignan.

关键词： Allamanda c athartica Tyrosinase Glabridin Kaempferol Naringenin Allamandicin

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Spatial evolution of the near-field distribution on planar gold nanoparticles with the excitation wavelength across dipole and quadrupole modes

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Photonics Research 2017年第3期5卷 187-193页

作者： JINGHUAN YANG QUAN SUN HAN YU kosei UENO HIROAKI MISAWA QIHUANG GONG State Key Laboratory for Mesoscopic Physics and Department of Physics Peking University Research Institute for Electronic Science Hokkaido University Collaborative Innovation Center of Quantum Matter

We investigate the superposition properties of the dipole and quadrupole plasmon modes in the near field both experimentally, by using photoemission electron microscopy(PEEM), and theoretically. In particular, the asymmetric near-field distributions on gold(Au) nanodisks and nanoblocks under oblique incidence with different polarizations are investigated in detail. The results of PEEM measurements show that the evolutions of the asymmetric near-field distributions are different between the excitation with s-polarized and p-polarized *** experimental results can be reproduced very well by numerical simulations and interpreted as the superposition of the dipole and quadrupole modes with the help of analytic calculations. Moreover, we hypothesize that the electrons collected by PEEM are mainly from the plasmonic hot spots located at the plane in the interface between the Au particles and the substrate in the PEEM experiments.

关键词： of mode Spatial evolution of the near-field distribution on planar gold nanoparticles with the excitation wavelength across dipole and quadrupole modes with in on is that

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Revealing the plasmon coupling in gold nanochains directly from the near field

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Opto-Electronic Advances 2019年第4期2卷 1-7页

作者： Quan Sun Han Yu kosei Ueno Shuai Zu Yasutaka Matsuo Hiroaki Misawa Research Institute for Electronic Science Hokkaido University Sapporo 001-0021 Japan College of Electronic Science and Engineering Jilin University Changchun 130012 China

We studied the near-field properties of localized surface plasmon resonances in finite linear gold nanochains using photoemission electron microscopy(PEEM).The localization of the electromagnetic field in the near-field region was mapped at high spatial *** tuning the excitation laser wavelength,we can obtain the near-field spectra,from which the energy splitting between longitudinal(L)and transverse(T)plasmon modes can be *** particular,the L-mode red shifts and the T-mode blue shifts with increasing chain *** red shift of the L-mode is highly dependent on the gap *** contrast,the T-mode almost remains constant within the range of gap distance we *** energy splitting between the L-mode and the T-mode of metallic chains is in agreement with previous far-field measurements,where it was explained by dipole-dipole near-field ***,we provide direct proof of this near-field plasmon coupling in nanochains via the above-described near-field measurements using *** addition,we explore the energy transport along the gold nanochains under excitation at oblique illumination via PEEM measurements together with numerical simulations.

关键词： surface plasmon resonance metallic nanochains near-field imaging photoemission electron microscopy

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Doublecortin and CaM kinase-like-1 as an independent prognostic factor in patients with resected pancreatic carcinoma

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World Journal of Gastroenterology 2017年第31期23卷 5764-5772页

作者： Kohei Nishio Kenjiro Kimura Ryosuke Amano Bunzo Nakata Sadaaki Yamazoe Go Ohira Kotaro Miura Naoki Kametani Hiroaki Tanaka Kazuya Muguruma kosei Hirakawa Masaichi Ohira Department of Surgical Oncology Osaka City University Graduate School of Medicine Department of Surgery Kashiwara Municipal Hospital

To elucidate the effect of expression of doublecortin and CaM kinase-like-1 (DCLK1) in patients with pancreatic ductal adenocarcinoma (PDAC). METHODSTumor specimens were obtained from 136 patients with pancreatic cancer who had undergone resection without preoperative therapy between January 2000 and December 2013 at the Department of Surgical Oncology, Osaka City University. The resected specimens were analyzed for associations with clinicopathological data, including DCLK1 expression, epithelial mesenchymal transition (EMT) marker expression, and cancer stem cell (CSC) marker expression. Univariate and multivariate survival analyses were performed and we assessed the association between DCLK1 expression and clinicopathological factors, including the EMT marker and CSC marker. RESULTSIn total, 48.5% (66/136) of the pancreatic cancer samples were positive for DCLK1. Patients with DCLK1-positive tumors had significantly shorter survival times than those with DCLK1-negative tumors (median, 18.7 mo vs 49.5 mo, respectively; P < 0.0001). Positive DCLK1 expression correlated with histological grade (P = 0.0290), preoperative CA19-9 level (P = 0.0060), epithelial cell adhesion molecule (EpCAM) expression (P = 0.0235), and the triple-positive expression of CD44/CD24/EpCAM (P = 0.0139). On univariate survival analysis, five factors were significantly associated with worse overall survival: histological grade of G2 to G4 (P = 0.0091), high preoperative serum SPan-1 level (P = 0.0034), R1/2 (P < 0.0001), positive expression of DCLK1 (P < 0.0001) or CD44 (P = 0.0245). On multivariate survival analysis, R1/2 [odds ratio (OR) = 2.019, 95% confidence interval (CI): 1.380-2.933; P = 0.0004] and positive DCLK1 expression (OR = 1.848, 95%CI: 1.2854-2.661; P = 0.0009) were independent prognostic factors. CONCLUSIONDCLK1 expression was found to be an independent prognostic factor and it may play a crucial prognostic role by promoting acquisition of stemness.

关键词： Doublecortin and CaM kinase-like-1 Pancreatic cancer Epithelial mesenchymal transition Cancer stem cell Prognostic factor

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Effect of Grains of Paradise (GP) Extract Intake on Obesity and Sympathetic Nerve Activity

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American Journal of Plant Sciences 2017年第2期8卷 85-95页

作者： Hiroyuki Hattori kosei Yamauchi Siaw Onwona-Agyeman Tohru Mitsunaga Faculty of Applied Biological Science Gifu University Gifu Japan Department of Medical Physiology Texas A&M Health Science Center College of Medicine Temple TX USA Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan

The methanol extract of grains of paradise (GP), the seed of Aframomum melegueta, which is distributed throughout West Africa, was administered during an animal breeding test. The extract suppressed body weight gain and decreased the weight of adipose tissues in breeding mice, with a greater effect on mice fed a high-fat diet (HFD) than on those fed a normal diet (ND). Other significant effects of GP intake included increased serum triglyceride (TG) concentration and reduced hepatic total cholesterol (TC) and TG concentrations. GP intake markedly prevented fat accumulation and improved hepatic lipid metabolism in HFD-fed mice. In addition, GP extract at a dosage of 5 mg/kg body weight decreased sympathetic nerve activity (SNA) in brown adipose tissue (BAT), while capsaicin, a major component of chili pepper, activated BAT SNA. This suggested that GP exerts a potential anti-obesity effect by a different mechanism from that of capsaicin.

关键词： Anti-Obesity Effect Grains of Paradise Aframomum melegueta Vanilloid BAT SNA

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Computer-Aided Design and Fabrication of Finger Prosthesis

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Journal of Biomedical Science and Engineering 2015年第2期8卷 98-103页

作者： Takeshi Murayama kosei Oono Mitsunori Tada Toru Eguchi Misuzu Nagami Mitsuhiro Tamamoto Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan Digital Human Research Center Agency for Industrial Science and Technology (AIST) Tokyo Japan Graduate School of Engineering Hiroshima University Hiroshima Japan Hello Tomorrow Japan Co. Ltd. Tokyo Japan

Custom-made esthetic finger prostheses, which are used for rehabilitation of patients with missing or impaired fingers, have been fabricated manually. However, such fabrication is time-consuming and requires manual skill. Here we propose a computer-aided method for fabricating finger pros-theses to save time and allow fabrications that do not require considerable manual skill. In this method, the dimensions of a patient’s healthy finger on the contralateral hand are first measured using a caliper. Using these dimensions, a three-dimensional model is constructed for fabricating a prosthesis for the patient’s impaired finger. Using the 3D model, a mold is designed using 3D modeling tools and a computer-aided design system. The resulting mold is then fabricated using a 3D printer. A finger prosthesis is fabricated by pouring silicone resin into the mold. A finger prosthesis for a volunteer was experimentally fabricated according to the proposed method. To evaluate the size and shape of the finger prosthesis, the difference between the finger prosthesis and the original finger of the volunteer was analyzed. Because the average difference between them was 0.25 mm, it was concluded that the proposed method could be used to fabricate a finger prosthesis of adequate size and shape.

关键词： Finger Prostheses Esthetic Prostheses Computer-Aided Design 3D Printer Additive Manufacturing

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