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Development of time-resolved immunofluorometric assays for the detection of house dust mite-allergic IgE in human sera

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Health 2010年第11期2卷 1280-1286页

作者： Ratchanoo Phiphatchaipaisarn Jundee Rabablert kornkarn bramarapravati Duangthep Thongdee Nares Wongpitoon Worawan Durongpisitkul Nat Malainual 不详

Dermatophagoides farinae and D. pteronyssinus are the prevalent house dust mites (HDM) in tropical countries and are associated with allergic diseases. This investigation developed a time- resolved immunofluorometric assay (TR-IFMA) for the first time to detect specific IgE antibody in patients with skin prick test positive to HDM but no detectable IgE by other means. Levels of IgE to natural and recombinant HDM allergens were measured by TR-IFMA in 50 HDM-allergic patients and 19 healthy participants compared to sandwich enzyme-linked immunosorbent assay (ELISA). A recombinant allergen, rDerf2, showed a 14 kDa band corresponding to broad range proteins of natural ***-IFMA showed sensitivity lower than 0.35 kUA/l. TR-IFMA employing three HDM antigens showed good correlations with sandwich ELISA at R2 0.93-0.96. TR-IFMA detected HDM IgE in 62, 62, 25 percent of allergic patient serum sample compared to 28, 32, and 22 percent detected by ELISA result using three HDM allergen. TR-IFMA also detected 26.3, 31.6, and 5.3 percent positive samples from 19 healthy participants while ELISA showed 0, 5.3, and 0 percents IgE positive samples. The use of rDerf2 as an HDM allergen for the assay was verified with no statistically different from other HDM allergens. TR-IFMA showed lower detection limit than ELISA and yielded higher sensitivity for serum of people with allergic symptoms with no detectable HDM IgE. It is anticipated that TR-IFMA for HDM-specific IgE detection will play an important role in future diagnosis of HDM allergy in clinical laboratories and for different research purposes.

关键词： House Dust Mites Time-Resolved Immunofluorometric Assay Allergy IgE

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Effectiveness of auricular point acupressure with magnetic plate for pain management in acute postpartum cesarean section patients in Thammasat University Hospital: a randomized clinical controlled trial

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Journal of Traditional Chinese Medicine 2022年第4期42卷 611-616页

作者： Rakchanok Tanitsookarn Komsun Suwannarurk Paradi Sangvatanakul Supapen Lertvuttivivat Junya Pattaraarchachai kornkarn Bhamarapravatana Department of obstetrics and gynecology Faculty of medicineThammasat UniversityPathum Thani 12120Thailand Chulabhorn International College of Medicine Thammasat UniversityPathum Thani 12120Thailand Department of preclinical science Faculty of medicineThammasat UniversityPathum Thani 12120Thailand

OBJECTIVE:To compare the effectiveness of auricular acupressure with or without magnetic plates to routine post cesarean pain ***:This randomized controlled trial was conducted at Obstetrics and Gynecology Department,Thammasat University Hospital,between January and June *** participants were term primigravida pregnant women who underwent cesarean delivery during the study *** were divided into three groups,namely control,placebo and study ***(magnetic application)and placebo(non-magnetic application)groups received magnetic and non-magnetic auricular patches at both pinnas,*** assignments for each group were computergenerated,printed and kept in opaque sealed *** points in this study were Shenmen(TF4),Erzhong(HX1)and Penqiang(TF5).Visual analog scale(VAS)was immediately recorded up to 72 h after the ***:A total of 195 cases were enrolled during the period of *** group had 65 *** mean participant’s age was 31 years *** and clinical characters among the three groups were *** scores within 12 h postoperative period of all three groups were *** 36 and 72 h post-operation,study groups had significantly lower VAS than the control ***-quarter of participants had comparable nausea and vomiting side ***:Auricular acupressure at Shenmen(TF4),Penqiang(TF5)and Erzhong(HX1)with magnetic plate attachment could significantly relieve post cesarean pain between 18 and 72 h.

关键词： cesarean section pain acupressure magnetic plate

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