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国际实验植物学杂志(英文) 2023年第10期92卷 2861-2874页

作者： Erick Malédon Elie Nudol Christophe Perrot marie-claire Gravillon Ronan Rivallan Denis Cornet Hâna Chair Komivi Dossa CIRAD UMR AGAP InstitutPetit BourgGuadeloupe97170France UMR AGAP Institut Univ MontpellierCIRADINRAEInstitut AgroMontpellierF-34398France CIRAD UMR AGAP InstitutMontpellierF-34398France

Various biological constraints including erratic and asynchronous flowering between male and female plants hinder successful hybrid development and genetic gains in greater yam breeding ***,pollen storage has gained much attention to facilitate artificial pollinations and increase the genetic *** 4-year study aimed at developing a practical long-term pollen storage technique for the successful development of yam *** pollens were collected from two Dioscorea alata males,then lyophilized(two lyophilization treatments were applied),followed by storage at room temperature(24℃–25℃)for 12 ***,the lyophilized and stored pollens were tested for viability by crossing with four female *** results showed that lyophilization is effective for achieving viable pollens after 12 months of *** 1(48 h drying)showed higher pollen germination and fertility rates than Treatment 2(72 h drying).Although we observed a reduction in viability of lyophilized pollens after 12 months of storage,we generated hybrid seedlings with success rates from 12%to 21%compared to 21%–31%when using fresh *** testing based on molecular genotyping confirmed the hybrid status of the obtained seedlings,which grew well in a *** is a practical approach for a long-term storage of greater yam pollen *** protocol will positively impact yam breeding programs particularly in developing countries.

关键词： Yam freeze-drying pollen conservation genetic gain

Mechanisms and treatment strategies of demyelinating and dysmyelinating Charcot-marie-Tooth disease

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Neural Regeneration Research 2023年第9期18卷 1931-1939页

作者： Nadège Hertzog claire Jacob Faculty of Biology Institute of Developmental Biology and NeurobiologyJohannes Gutenberg University MainzHanns-Dieter-Hüsch-WegMainzGermany

Schwann cells,the myelinating glia of the peripheral nervous system,wrap axons multiple times to build their myelin *** is of paramount importance for axonal integrity and fast axon potential ***,myelin is lacking or dysfunctional in several neuropathies including demyelinating and dysmyelinating Charcot-M arie-To oth ***-marie-To oth disease represents the most prevalent inherited neuropathy in humans and is classified either as axonal,demyelinating or dysmyelinating,or as *** demyelinating or dysmyelinating forms of Charcot-marie-Tooth disease constitute the majority of the disease cases and are most frequently due to mutations in the three following myelin genes:peripheral myelin protein 22,myelin protein ze ro and gap junction beta 1(coding for Connexin 32) causing Charcot-M arie-Tooth disease type 1A,Charcot-marie-Tooth disease type 1B,and X-linked Charcot-M arie-Tooth disease type 1,*** resulting perturbation of myelin structure and function leads to axonal demyelination or dysmyelination and causes severe disabilities in affected *** treatment to cure or slow down the disease progression is currently available on the market,howeve r,scientific discoveries led to a better understanding of the pathomechanisms of the disease and to potential treatment *** this review,we describe the features and molecular mechanisms of the three main demyelinating or dysmyelinating forms of Charcot-marie-Tooth disease,the rodent models used in research,and the emerging therapeutic approaches to cure or counteract the progression of the disease.

关键词： Charcot-marie-Tooth disease rodent models emerging treatments demyelination and dysmyelination endoplasmic reticulum stress gene therapy myelin repair Schwann cells unfolded protein response

Epididymis-specific lipocalin promoters

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Asian Journal of Andrology 2007年第4期9卷 515-521页

作者： Kichiya Suzuki Xiuping Yu Pierre Chaurand Yoshihiko Araki Jean-Jacques Lareyre Richard M. Caprioli marie-claire Orgebin-Crist Robert J. Matusik Department of Obstetrics and Gynecology Tohoku University Graduate School of Medicine Sendai 980-8574 Japan Departments of Urologic Surgery Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departments of Biochemistry Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departments of Obstetrics and Gynecology Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departments of Cell and Developmental Biology Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departments of Mass Spectrometry Research Center and Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departments of Center for Reproductive Biology Research Vanderbilt University School of Medicine Nashville Tennessee 37232 USA

Our goal is to decipher which DNA sequences are required for tissue-specific expression of epididymal genes. At least 6 epididymis-specific lipocalin genes are known. These are differently regulated and regionalized in the epididymis. Lipocalin 5 （Lcn5 or mE-RABP） and Lipocalin 8 （Lcn8 or mEP17） are homologous genes belonging to the epididymis-specific lipocalin gene cluster. Both the 5 kb promoter fragment of the Lcn5 gene and the 5.3 kb promoter fragment of the Lcn8 gene can direct transgene expression in the epididymis （Lcn5 to the distal caput and Lcn8 to the initial segment）, indicating that these promoter fragments contain important cis-regulatory element（s） for epididymisspecific gene expression. To define further the fragments regulating gene expression, the Lcn5 promoter was examined in transgenic mice and immortalized epididymal cell lines. After serial deletion, the 1.8 kb promoter fragment of the Lcn5 gene was sufficient for tissue-specific and region-specific gene expression in transgenic mice. Transient transfection analysis revealed that a transcription factor forkhead box A2 （Foxa2） interacts with androgen receptor and binds to the 100 bp fragment of the Lcn5 promoter between 1.2 kb and 1.3 kb and that Foxa2 expression inhibits androgen-dependent induction of the Lcn5 promoter activity. Immunohistochemistry indicated a restricted expression of Foxa2 in the epididymis where endogenous Lcn5 gene expression is suppressed and that the Foxa2 inhibition of the Lcn5 promoter is consistent with the lack of expression of Lcn5 in the corpus and cauda. Our approach provides a basic strategy for further analysis of the epididymal lipocalin gene regulation and flexible control of epididymal function. （Asian J Androl 2007 July; 9： 515-521）

关键词： androgen epididymis gene cluster gene evolution gene regulation lipocalin transcription factor

Mucosal healing progression after acute colitis in mice

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World Journal of Gastroenterology 2019年第27期25卷 3572-3589页

作者： Sandra Vidal-Lletjós Mireille Andriamihaja Anne Blais Marta Grauso Patricia Lepage Anne-marie Davila claire Gaudichon Marion Leclerc Francois Blachier Annaig Lan UMR PNCA AgroParisTechINRAUniversite Paris-SaclayParis 75005France UMR MICALIS INRAUniversite Paris-SaclayJouy-en-Josas 78350France

BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required. These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins. However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little *** To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced *** C57 BL/6 male mice were given 3.5% dextran sodium sulfate(DSS) in drinking water for 5 d. They were euthanized 2(day 7), 5(day 10), 8(day 13), and 23(day28) d after DSS removal. The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0. Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16 S rRNA *** The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa. It was recorded 2 d after termination of the DSStreatment, followed by a progressive return to values similar to those of control mice. Although signs of colitis were severe(inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations(hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammat

关键词： Colon luminal environment Dextran sodium sulfate-induced colitis Dysbiosis Epithelial repair Acute colitis

生态旅游对当地社区和森林覆盖变化的影响 ——以卢旺达火山国家公园为例

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生态旅游对当地社区和森林覆盖变化的影响 ...

作者： marie claire Uwineza 北京林业大学

学位级别：硕士

生态旅游具有参与自然保护区的保护和为当地社区提供利益的双重功效。这项VNP研究的目的是通过提高森林覆盖率,评估该地区的生态旅游对当地社区的收入和VNP保护的影响。数据来源于100名当地居民,他们从事与生态旅游相关的活动和其他职... 详细信息

生态旅游具有参与自然保护区的保护和为当地社区提供利益的双重功效。这项VNP研究的目的是通过提高森林覆盖率,评估该地区的生态旅游对当地社区的收入和VNP保护的影响。数据来源于100名当地居民,他们从事与生态旅游相关的活动和其他职业。为了探究生态旅游对森林覆盖率变化的影响,分析了3张卫星图像,1986年的图像用于评估实施严格的生态旅游保护措施之前的森林覆盖率,2003年的图像用于评估实施严格的生态旅游保护措施开始时的森林覆盖率,2019年的图像用于评估现状。结果表明,生态旅游对当地社区的收入具有积极影响,可为当地居民带来直接和间接的利益。例如,通过提供月薪就业机会提供直接支持,并通过建设卫生中心、道路、学校和水泵等社区基础设施提供间接支持。结果表明,引入生态旅游前社区居民的年收入与当前居民的年收入存在显著的差异（p=0.03）。此外,研究发现,从生态旅游中获得的利益导致社区对VNP的保护态度发生变化。卫星图像的结果表明,引入严格的生态旅游保护措施对森林覆盖率的改变（新造林）有积极的影响,每年的变化率为0.23%,但是这个变化速率很低,建议在未来进行更多的研究来验证生态旅游是否会对森林覆盖变化产生显著的积极影响。综上所述,有证据表明,生态旅游对当地社区和森林覆盖变化具有积极的影响。

关键词：卢旺达火山国家公园生态旅游的影响公园保护森林保护

A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals：A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis

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Journal of Genetics and Genomics 2016年第5期43卷 341-348页

作者： Vanessa Chenouard Lucas Brusselle Jean-marie Heslan Séverine Remy Séverine Ménoret claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson Institut National de la Sante'Et de la Recherche Medicale UMR 1064-1nstitut Transplantation Urologie-Nephrologie Centre Hospitalier Universitaire de Nantes Nantes 44093 France Transgenesis Rat ImmunoPhenomic Platform Structure Federative de Recherche Frangois Bonamy Centre National de Recherche Scientifique UMS3556 Nantes 44093 France GenoCellEdit INSERM UMR 1064-ITUN CHU Nantes Nantes 44093 France

The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of ***,these novel tools have become easier to use and have resulted in a great increase of *** gene knockout（KO） or knockin（KI） animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these *** several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping *** HMA-CE method uses a simple PCR amplification of genomic DNA（gDNA） followed by an automated capillary electrophoresis step which reveals a heteroduplexes（HD） signature for each *** allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level.

关键词： Genome editing Genotyping Heteroduplex mobility assay Capillary electrophoresis

Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy

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Light(Science & Applications) 2023年第9期12卷 1782-1794页

作者： Clothilde Raoux Anatole Chessel Pierre Mahou Gaël Latour marie-claire Schanne-Klein Laboratory for Optics and Biosciences Ecole PolytechniqueCNRSINSERMInstitut Polytechnique de Paris91128 PalaiseauFrance UniversitéParis-Saclay 91190 Gif-sur-YvetteFrance

A key property of the human cornea is to maintain its curvature and consequently its refraction capability despite daily changes in intraocular *** is closely related to the multiscale structure of the corneal stroma,which consists of 1-3µm-thick stacked lamellae made of thin collagen ***,the distribution,size,and orientation of these lamellae along the depth of the cornea are poorly characterized up to *** this study,we use second harmonic generation(SHG)microscopy to visualize the collagen distribution over the full depth of 10 intact and unstained human corneas(500-600µm thick).We take advantage of the small coherence length in epi-detection to axially resolve the lamellae while maintaining the corneal physiological ***,as raw epi-detected SHG images are spatially homogenous because of the sub-wavelength size of stromal collagen fibrils,we use a polarimetric approach to measure the collagen orientation in every *** a careful validation of this approach,we show that the collagen lamellae(i)are mostly oriented along the inferior-superior axis in the anterior stroma and along the nasal-temporal axis in the posterior stroma,with a gradual shift in between and(ii)exhibit more disorder in the anterior *** results represent the first quantitative characterization of the lamellar structure of the human cornea continuously along its entire thickness with micrometric *** also shows the unique potential of P-SHG microscopy for imaging of collagen distribution in thick dense tissues.

关键词： structure curvature resolved

Expression Patterns of CAPN1 and CAPN8b Genes during Embryogenesis in Xenopus laevis

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CellBio 2013年第4期2卷 211-216页

作者： Lucie Abrouk-Vérot claire Brun Jean-marie Exbrayat Université de Lyon UMRS 449 Biologie Générale Université Catholique de Lyon Reproduction et Développement Comparé Ecole Pratique des Hautes Etudes Lyon France

Calpains are a superfamily of Ca2+-dependent cysteine proteases, implicated in various cellular processes and thus probably necessary in all the stages of cell life. The first extended report of quantification of total RNAs within the developmental stages of Xenopus laevis was described in this study. Decreases of total RNAs were positively associated with waves of apoptotic cell death (onset of gastrulation, and morphogenesis). Using qPCR, the temporal expression pattern of CAPN1 and CPAN8b (XCL-2) were characterized during the Xenopus laevis embryogenesis. Transcripts of the CAPN1 and CAPN8 genes were detectable from gastrula stage and their levels oscillated throughout development. The expression of the CAPN1 (mu/I) gene was observed in earliest stage, indicating a maternal origin, while expression of the CAPN8b gene was detectable after midblastula transition. The levels of the two transcripts then started to rise again obviously as a result of zygotic expression (stage 11). The CAPN1 gene expression was particularly expressed at tailbud stage, while the CAPN8 transcripts were found at gastrula, neurula and tailbud stages. This is the first report of quantification of mRNAs CAPN8b and CAPN1 (mu/I) within the developmental stages of Xenopus laevis by qPCR.

关键词： Calpain Embryonic Development Cell Death Xenopus laevis

Analysis of Sodium and Potassium in Total Parenteral Nutrition Bags by ICP-MS and ICP-AES:Critical Influence of the Ingredients

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American Journal of Analytical Chemistry 2011年第5期2卷 573-581页

作者： Nicolas marie claire Verdier Barbara Le Bot Gwenola Burgot 不详

The compounding of total parenteral nutrition solutions (TPN) in the hospital pharmacy is a high-risk activity for which a quality assurance programme is necessary. The complexity of parenteral nutrition solutions containing almost 50 ingredients makes it difficult to measure each of them. On the other hand, the assay of electrolytes such as sodium and potassium is accepted as a quality marker for estimating compounding errors. Thus, the aim of this study was to estimate the influence of ingredients on the accuracy of assays of electrolytes. Experiments were performed with aqueous working simulated solutions of sodium and potassium prepared by the addition of each nutrient step by step, (dextrose, amino acids, lipids, vitamins and trace elements). Sodium and potassium levels were measured by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) and Atomic Emission Spectroscopy (ICP-AES). The performance of these methods was compared using statistical evaluations (t-test and Mann–Whitney test).The study highlights the interference of amino acids, vitamins and trace elements when measuring sodium, but no interference was noted during the measurement of potassium. To reduce the risk and to improve the quality of compounding, we used an automated compounding device but, even in this case, the acceptance criterion for sodium and potassium determination was not <10%.

关键词： Inorganic Cations Electrolytes Total Parenteral Nutrition Atomic Emission Spectrometry

Carbon pool dynamics after variable retention harvesting in Nothofagus pumilio forests of Tierra del Fuego

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Ecological Processes 2023年第1期12卷 64-82页

作者： Jimena E.Chaves marie-claire Aravena Acuña Julián Rodríguez-Souilla Juan M.Cellini Nolan J.Rappa María V.Lencinas Pablo L.Peri Guillermo J.Martínez Pastur Centro Austral de Investigaciones Científicas(CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Houssay 200(9410)UshuaiaTierra del FuegoArgentina Laboratorio de Investigaciones en Maderas(LIMAD) Universidad Nacional de la Plata(UNLP)Diagonal 113469(1900)La PlataBuenos AiresArgentina Nature Conservation&Landscape Ecology University of FreiburgTennenbacherstrasse 479106 FreiburgGermany Instituto Nacional de Tecnología Agropecuaria(INTA) Universidad Nacional de la Patagonia Austral(UNPA)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Cc 332(9400)Río GallegosSanta CruzArgentina

Background It is necessary to determine the implications for managing forest stands using variable retention harvesting for maintaining carbon and for calculating the effects of different harvesting practices on above-and below-ground carbon balance in forest *** this context,forest carbon management has gained more attention among managers and policy-makers during recent *** aim of this study was to determine carbon pool dynamics in different forest ecosystem components after variable retention harvesting(VRH)to characterize the ecological stability and quantify the recovery rate through the years-after-harvesting(YAH).Methods Carbon pool compartmentalization of 14 different components was determined in 60 harvested and primary unmanaged forests during the first 18 YAH in Tierra del Fuego(Argentina).We compared them using uni-and multi-variate methods,relativizing the outputs with primary unmanaged *** We determined the effectiveness to retain carbon components in post-harvested stands under different retention strategies(aggregated ***).The balance among carbon pool components changed between managed and unmanaged stands across the YAH,and was directly related to the impact *** retention improved the ecological stability of the harvested areas,where the below-ground components were more stable than the above-ground *** recovery rate was directly related to the post-harvesting natural dynamics of the *** studied period was not enough to fully recover the C levels of primary unmanaged for-ests,but VRH showed advantages to increase the C pools in the managed *** Promoting VRH can improve sustainable forestry at the landscape level and in the long term,generating positive synergies with biodiversity and the provision of ecosystem *** study provides important new insights into forest carbon management,in particular to setting standards in carbon projects and sets the ground

关键词： Carbon reservoir Temperate forest Ecological stability Recovery rate Forest carbon management