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Phytochemical analysis of Berberis lyceum methanolic extract and its antiviral activity through the restoration of MAPK signaling pathway modulated by HCV NS5A

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Asian Pacific Journal of Tropical Biomedicine 2021年第3期11卷 132-140页

作者： koloko brice landry Shafiq Azam Sidra Rehman Somayya Tariq Bushra Iqbal Mateen Abbas Dieudonné Massoma Lembè Bushra Ijaz Laboratory of Applied and Functional Genomic Centre of Excellence in Molecular BiologyUniversity of the PunjabLahorePakistan Department of Biosciences COMSATS University IslamabadIslamabadPakistan Quality Operations Laboratory University of Veterinary and Animal SciencesLahorePakistan Laboratory of Animal Biology and Physiology Faculty of ScienceUniversity of DoualaDoualaCameroon

Objective:To evaluate the antiviral activity and phytochemicals of selected plant extracts and their effect on the mitogen-activated protein kinase(MAPK)signaling pathway modulated by hepatitis C virus(HCV)nonstructural protein 5 A(NS5A).Methods:A total of ten plant extracts were initially screened for their toxicities against Hep G2 *** non-toxic plants were tested for their inhibitory effect on the expression of HCV NS5A at both m RNA and protein levels using real-time PCR and Western blotting assays,*** differential expression of the genes associated with MAPK pathway in the presence of NS5A gene and plant extract was measured through real-time ***,the identification of secondary metabolites was carried out by phytochemical and HPLC ***:The phytochemical profiling of Berberis lyceum revealed the presence of alkaloids,phenols,saponins,tannins,flavonoids,carbohydrates,terpenoids,steroids,and ***,quercetin,myricetin,gallic acid,caffeic acid,and ferulic acid were identified through HPLC *** methanolic extract of Berberis lyceum strongly inhibited HCV RNA replication with an IC50 of 11.44μg/m ***-PCR and Western blotting assays showed that the extract reduced the expression of HCV NS5A in a dosedependent *** lyceum extract also attenuated NS5A-induced dysregulation of the MAPK signaling ***:Our findings suggest that Berberis lyceum extract strongly inhibits HCV propagation by reducing HCV NS5A-induced perturbation of MAPK signaling.

关键词： Anti-HCV HCV NS5A Berberis lyceum MAPK pathway Phytochemicals HPLC

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