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Analysis on Genetic Diversity of 40 Flowering Cherry Cultivars and Construction of Molecular ID Based on SSR Markers

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Agricultural Sciences 2024年第2期15卷 256-273页

作者： Chaoren Nie Xiaoguo Xu Xiaoqin Zhang hongbing sun Jingya Yu Wensheng Xia Wuhan Institute of Landscape Architecture Wuhan China Wuhan Landscape Ecology Group Co. Ltd. Wuhan China

Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. however, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, Na and Ne were 6.062, 4.326, respectively. Ho and He were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest Ho, He, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.

关键词： Flowering Cherry SSR Genetic Relationship Molecular ID Identifying Cultivars

Tunable ultraviolet to deep blue light emission from sulfur nanodots fabricated by a controllable fission-aggregation strategy

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Science China Materials 2022年第10期65卷 2786-2792页

作者： Lian Xiao Quanchao Du Yi Huang Shijia Cheng Shuai Yin Teck Neng Wong Edwin kok Lee Yeow Handong sun Division of Physics and Applied Physics School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore 637371Singapore Division of Chemistry and Biological Chemistry School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore 637371Singapore Research Institute of Aero-Engine Beihang UniversityBeijing 100083China School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore MajuLab International Joint Research Unit UMI 3654CNRSUniversitéCôte d’AzurSorbonne UniversitéNational University of SingaporeNanyang Technological UniversitySingapore 637371Singapore

Bright tunable light emission in the short wavelength range from sulfur nanodots was demonstrated with a photoluminescence quantum yield(PLQY)of up to 59.4%.A fission-aggregation mechanism was proposed for the formation of sulfur nanodots with desired *** synthetic strategy allowed for simultaneous size control from 3.2 to 5.6 nm,thus tuning the emission color from ultraviolet(UV)to deep blue(342±430 nm),and for the suppression of unwanted nonradiative recombination centers and deep level *** luminescence mechanism and quantum confinement effect of the synthesized sulfur nanodots were investigated by optical spectroscopy and theoretical *** results show promise toward the application of sulfur nanodots in UV optoelectronics,biomedical treatments,and sterilization.

关键词： sulfur nanodots ultraviolet deep blue tunable photoluminescence heavy metal free high PLQY

Spatiotemporal Variations and Controls on Anthropogenic Heat Fluxes in 12 Selected Cities in the Eastern China

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Chinese Geographical Science 2021年第3期31卷 444-458页

作者： CAO Zheng WEN Ya SONG Song HUNG Chak ho sun Hui School of Geographical Sciences Guangzhou UniversityGuangzhou 510006China Guangdong Province Engineering Technology Research Center for Geographical Conditions Monitoring and Comprehensive Analysis Guangzhou 510006China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China College of Resources and Environment South China Agricultural UniversityGuangzhou 510642China S.Department of Urban Planning and Design The University of Hong KongHong Kong 999077China

Spatiotemporal variations of anthropogenic heat flux(AHF)is reported to be associated with global ***,confined to the low spatial resolution of energy consumption statistical data,details of AHF was not well *** obtain high spatial resolution data of AHF,Defense Meteorological Satellite Program/Operational Linescan System(DMSP/OLS)nighttime light time-series product and Moderate Resolution Imaging Spectroradiometer(MODIS)satellite monthly normalized difference vegetation index(NDVI)product were applied to construct the human settlement *** on the spatial regression relationship between human settlement index and energy consumption data.A 1-km resolution dataset of AHF of 12 selected cities in the eastern China was *** least-squares(OLS)model was applied to detect the mechanism of spatial patterns of *** showed that industrial emission in selected cities of the eastern China was accountable for 63%of the total *** emission in megacities,such as Tianjin,Jinan,Qingdao,and Hangzhou,was most *** increasing speed in most areas in the chosen cities was quite *** growth or extremely high growth of AHF were located in central downtown *** Beijing,Shanghai,Guangzhou,Jinan,Hangzhou,Changzhou,Zhaoqing,and Jiangmen,a single kernel of AHF was *** influencing factors showed that precipitation,temperature,elevation,normalized different vegetation index,gross domestic product,and urbanization level were positive with ***,this investigation implied that urbanization level and economic development level might dominate the increasing of AHF and the spatial heterogeneousness of *** urbanization level or economic development level resulted in high increasing speeds of *** findings provide a novel way to reconstruct of AHF and scientific supports for energy management strategy development.

关键词： anthropogenic heat flux(AHF) Defense Meteorological Program/Operational Linescan System(DMSP/OLS)data spatiotemporal variations influencing factors eastern China

Applying Petri-Net-Based Reduction Approach for Verifying the Correctness of Workflow Models

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Wuhan University Journal of Natural Sciences 2006年第1期11卷 203-210页

作者： LI Xi-zuo HAN Gui-ying KIM sun-ho School of Computer Science and Engineering Dalian Nationalities University Dalian 116600 Liaoning China School of Electromechanical Engineering Dalian Nationalities University Dalian 116600 Liaoning China Department of Industrial and Systems Engineering Myongji University San 38-2 Namdong Yonginsi Kyunggido 449 728Korea

The correctness of workflow models is one of the major challenges in context of workflow analysis. The aim of this paper is to provide an improved Petri net-based reduction approach for verifying the correctness of workflow models. To the end, how to represent well-behaved building blocks and control structures of business processes by Petri nets is given at first, and then how to build well-structured process nets is presented. According to the structural characteristics of well-structured process nets, a set of legacy reduction rules are improved and extended, and then a complete Petri-net-based verification approach is proposed. The sound ness and the complexity with polynomial time for the improved re duction method are also proven.

关键词： workflow analysis well structured process nets Petri nets reduction rules

The Molecular Mechanism of Interaction between Sushi Peptide and Pseudomonas Endotoxin

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Cellular & Molecular Immunology 2006年第1期3卷 21-28页

作者： Peng Li Miao sun Thorsten Wohland Bow ho Jeak Ling Ding Departments of Biological Sciences National University of Singapore Singapore 1175431Singapore Departments of Chemistry National University of Singapore Singapore 117543 Singapore Departments of Microbiology National University of Singapore Singapore 117543 Singapore Departments of Biological Sciences and Microbiology National University of Singapore 14 Science Drive 4 Singapore 117543 Singapore

Septic shock is caused by Gram-negative bacterial infection. Lipopolysaccharide （LPS） is the bioactive molecule present on the outer membrane of the Gram-negative bacteria. It is generally thought that LPS interacts with sensors on the host cell membrane to activate the intracellular signaling pathway resulting in the overproduction of cytokines such as TNF-α This causes inflammation and ultimately, septic shock. Lipid A is the pharmacophore of the LPS molecule. Thus, developing bio-molecules which are capable of binding LPS at high affinity, especially to the lipid A moiety is an efficient way to neutralize the LPS toxicity. Factor C, a serine protease in the horseshoe crab ameobocytes, is sensitive to trace levels of LPS. We have derived Sushi peptides from the LPS-binding domains of Factor C. Our earlier study showed that the Sushi peptides inhibit LPS-induced septic shock in mice. Here, we demonstrate that the molecular interaction between LPS and Sushi 1 peptide is supported by the hydrophobic interaction between the lipid tail of LPS and Sushi 1 peptide. Furthermore, in the presence of LPS, the peptide transitions from a random structure into an α-helical conformation and it disrupts LPS aggregates, hence, neutralizing the LPS toxicity.

关键词： septic shock Sushi 1 peptide Factor C Pseudomonas endotoxin/LPS LPS binding electrostatic and hydrophobic interaction

Automatic Discontinuity Classification of Wind-turbine Blades Using A-scan-based Convolutional Neural Network

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Journal of Modern Power Systems and Clean Energy 2021年第1期9卷 210-218页

作者： Jiyeon Choung sun Lim Seung Hwan Lim Su Chung Chi Mun ho Nam Korea Electronics Technology Institute 401-402Bucheon Technopark655Pyeongcheon-roWonmi-guBucheon-siGyeonggi-do 14502Korea Sam Yong Inspection Engineering Co.Ltd.

Recent development trends in wind power generation have increased the importance of the safe operation of wind-turbine blades(WTBs). To realize this objective, it is essential to inspect WTBs for any defects before they are placed into operation. however, conventional methods of fault inspection in WTBs can be rather difficult to implement, since complex curvatures that characterize the WTB structures must ensure accurate and reliable inspection. Moreover, it is considered useful if inspection results can be objectively and consistently classified and analyzed by an automated system and not by the subjective judgment of an inspector. To address this concern,the construction of a pressure-and shape-adaptive phased-array ultrasonic testing platform, which is controlled by a nanoengine operation system to inspect WTBs for internal defects, has been presented in this paper. An automatic classifier has been designed to detect discontinuities in WTBs by using an A-scanimaging-based convolutional neural network(CNN). The proposed CNN classifier design demonstrates a classification accuracy of nearly 99%. Results of the study demonstrate that the proposed CNN classifier is capable of automatically classifying the discontinuities of WTB with high accuracy, all of which could be considered as defect candidates.

关键词： Wind-turbine blade(WTB) blade inspection platform convolutional neural network(CNN) discontinuity phased-array ultrasonic testing(PAUT) A-scan

A study on effects of ceramic foam filter on flow aspect through water modeling experiment

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China Foundry 2017年第3期14卷 216-225页

作者： ho-Young Hwang Cheol-Hee Nam Young-Sim Choi Jun-ho hong Xun sun Cyber Manufacturing Process Group Korea Institute of Industrial Technology Incheon 21999 South Korea Department of Robotics and Virtual Engineering Korea University of Science and Technology Daejeon 34113 South Korea Materials Science and Engineering Kyoto University Kyoto 606-8501 Japan Shenyang Research Institute of Foundry Shenyang 110022 China

Casting defects that are closely related to entrapped air bubbles and metallic oxides occur very frequently in the sand mold casting process. Many researchers have shown that these defects can be reduced by adopting an appropriate gating system design. however, it is difficult for field engineers to identify a specific gating system that is more appropriate for their products. In this study, we tried to draw a comparison between two gating system designs with and without a ceramic foam filter. The ceramic foam filter was added to the horizontal runner just after the down sprue to prevent air bubble generation and reduce turbulence without a change of gating system design. The water modeling experiment was conducted with four different amounts of the initial volumes of water in the reservoir to verify the effects of initial pouring velocity. The results of the experiment applying the filter showed remarkably changed flow characteristics. The use of the filter was found to convert the flow pattern of water in the desired direction. The ceramic foam filter performed well to reduce flow velocity and stabilize the water *** flow pattern without a filter can be improved significantly even with the the use of just a 10 PPI irregular filter. Although the study confirmed that use of the filter may change the flow characteristics, it needs to be noted that the use of the ceramic filter alone cannot solve all the problems caused by a poorly designed gating system.

关键词： ceramic foam filter sprue gating system design sand mold casting casting defect

Revision of Senegalia in China, and notes on introduced species of Acacia, Acaciella, Senegalia and Vachellia(Leguminosae: Mimosoideae)

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Plant Diversity 2019年第6期41卷 353-480页

作者： Bruce R.Maslin Boon Chuan ho Hang sun Lin Bai Research Associate of Western Australian Herbarium Department of BiodiversityConservation and AttractionsBentley Delivery CentrePO Box 104WA6983Australia Research Associate of Singapore Botanic Gardens Herbarium Republic of Singapore Herbarium Singapore Botanic GardensNational Parks Board1 Cluny Road259569Republic of Singapore CAS Key Laboratory of Biodiversity and Biogeography Kunming Institute of BotanyChinese Academy of SciencesKunming650201China

The present work represents the first comprehensive taxonomic revision of Acacia ***.(Leguminosae:Mimosoideae)in China,focusing on the indigenous *** reassessments of specimens and re-defin让ion of previously recorded species have resulted in recognising 21 indigenous species under Senegalia,a segregate of the former Acacia *** botanical profiles are provided for these species,together w让h a diagnostic line drawing and distribution map;photographs of living plants are provided for more than half the *** treatments are also provided for the 15 species from four genera that are regarded as major introductions of Acacia *** the country:Acacia Mill.(12 spp.),Acaciella Britton&Rose(1 sp.),Senegalia Raf.(1 sp.)and Vachellia Wight&Arn.(1 sp.).An identification key to all Acacia *** in China is *** indigenous species of Senegalia in China belong to ***(Vassal)Maslin,comb,nov.,and the introduced ones to *** a consequence of this study,the number of Senegalia species now recognised for China has almost *** new species are described:Senegalia clandestina Maslin,***,***&LBai,***.;Senegalia guangdongensis Maslin,***,***&LBai,***.;Senegalia obliqua Maslin,***,***&LBai,***.;Senegalia orientalis Maslin,***,***&LBai,***.;Senegalia prominens Maslin,***,***&LBai,***.;Senegalia stipitata Maslin,***,***&LBai,*** new combinations are established:Senegalia garrettii(***)Maslin,***,***&LBai,comb.&stat,nov.;Senegalia kerrii(***)Maslin,***,***&LBai,comb.&stat,nov.;Senegalia kunmingensis(***&***)Maslin,***,***&LBai,comb.&stat,nov.;Senegalia macrocephala(Lace)Maslin,***,***&LBai,*** andamanica(***)Maslin,Seigler&Ebinger and *** are new records for China.A lectotype is designated for Acacia teniana Harms and 2nd step lectotypifications effected for Acacia delavayi

关键词： Acacia Acaciella Senegalia Vachellia Taxonomy China

Biodegradable nanocarriers for small interfering ribonucleic acid （siRNA） co-delivery strategy increase the chemosensitivity of pancreatic cancer cells to gemcitabine

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Nano Research 2017年第9期10卷 3049-3067页

作者： Chengbin Yang kok Ken Chan Wen-Jen Lin Alana Mauluidy Soehartono Guimiao Lin Huiting Toh ho Sup Yoon Chih-Kuang Chen Ken-Tye Yong School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore Polymeric Biomaterials Lab Department of Fiber and Composite Materials Feng Chia University Taichung 40724 Taiwan China Key Lab of Biomedical Engineering School of Medicine Shenzhen University Shenzhen 518060 China Division of Structural Biology & Biochemistry School of Biological Sciences Nanyang Technological University Singapore 639798Singapore Department of Genetic Engineering College of Life Sciences Kyung Hee University Yongin-si Gyeonggi-do 446701 Republic of Korea

Ribonucleic acid (RNA) interference (RNAi) therapies are promising cancer treatment modalities that can specifically target abnormal proto-oncogenes, thus improving the therapeutic effect. For the treatment of pancreatic cancer, targeting one mutant proto-oncogene by RNAi usually does not yield the desired therapeutic efficiency. Both K-ras gene mutations and Notch1 overexpression are common symptoms in pancreatic cancer patients, and play a crucial role in pancreatic cancer cell drug resistance. In this study, biodegradable charged polyester-based vectors (BCPVs) were synthesized for the co-delivery of K-ras and Notch1 small interfering ribonucleic acid (siRNA) into MiaPaCa-2 cells (pancreatic cancer cell line) to overcome drug resistance to gemcitabine (GEM), a first-line chemotherapeutic drug used in the clinic. BCPVs could effectively absorb negative siRNA to form a capsule-like structure, prevent siRNA from nuclease digestion in the serum, and promote effective siRNA cell internalization and endosomal escape. Through K-ras and Notch1 gene silencing in MiaPaCa-2 cells, BCPV-siRNAK-ras-siRNANotch1 nanocomplexes effectively reversed the epithelia-mesenchymal transition (EMT) in MiaPaCa-2 cells, thereby greatly enhancing the sensitivity of MiaPaCa-2 cells to GEM. MiaPaCa-2 cell proliferation, migration, and invasion were effectively inhibited, and cell apoptosis was also significantly enhanced by the synergistic antitumor effect of BCPV-siRNAK-ras-siRNANotch1 nanocomplexes and GEM. These results suggest that this combination RNAi therapy can be used to improve cancer cell sensitivity to chemotherapeutic drugs. Specifically, this newly developed strategy has a great potential for treating pancreatic cancer.

关键词： pancreatic cancer small interfering ribonucleic acid (siRNA) biodegradable charged polyester-based vector (BCPV) gemcitabine (GEM) epithelia-mesenchymal transition (EMT)