检索结果-南通市图书馆

Effects of palbociclib on oral squamous cell carcinoma and the role of PIK3CA in conferring resistance

Cancer Biology & Medicine 2019年第2期16卷 264-275页

作者： Nur Syafinaz Zainal Bernard kok Bang lee Zheng Wei Wong Iuan Sheau Chin Pei San Yee Chai Phei Gan Kein Seong Mun Zainal Ariff Abdul Rahman J. Silvio Gutkind Vyomesh Patel Sok Ching Cheong Head and Neck Team Cancer Research Malaysia Selangor 47500 Malaysia Department of Oral & Maxillofacial Clinical Sciences Faculty of Dentistry University of Malaya Kuala Lumpur 50603 Malaysia Department of Pathology Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia Oral Cancer Research and Co-ordinating Centre (OCRCC) Faculty of Dentistry University of Malaya Kuala Lumpur 50603 Malaysia Department of Pharmacology University of California San Diego 92093-5004 CA USA

Objective: Lack of effective therapies remains a problem in the treatment of oral squamous cell carcinoma(OSCC), especially in patients with advanced tumors. OSCC development is driven by multiple aberrancies within the cell cycle pathway, including amplification of cyclin D1 and loss of p16. Hence, cell cycle inhibitors of the CDK4/6-cyclin D axis are appealing targets for OSCC treatment. Here, we determined the potency of palbociclib and identified genetic features that are associated with the response of palbociclib in ***: The effect of palbociclib was evaluated in a panel of well-characterized OSCC cell lines by cell proliferation assays and further confirmed by in vivo evaluation in xenograft models. PIK3CA-mutant isogenic cell lines were used to investigate the effect of PIK3CA mutation towards palbociclib ***: We demonstrated that 80% of OSCC cell lines are sensitive to palbociclib at sub-micromolar ***, palbociclib was effective in controlling tumor growth in mice. We identified that palbociclib-resistant cells harbored mutations in PIK3CA. Using isogenic cell lines, we showed that PIK3CA mutant cells are less responsive to palbociclib as compared to wild-type cells with concurrent upregulation of CDK2 and cyclin E1 protein levels. We further demonstrated that the combination of a PI3 K/mTOR inhibitor(PF-04691502) and palbociclib completely controlled tumor growth in ***: This study demonstrated the potency of palbociclib in OSCC models and provides a rationale for the inclusion of PIK3CA testing in the clinical evaluation of CDK4/6 inhibitors and suggests combination approaches for further clinical studies.

关键词： OSCC palbociclib CDK4/6 inhibitors PIK3CA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Solving the benign prostatic hyperplasia puzzle

引用

Asian Journal of Urology 2016年第1期3卷 6-9页

作者： Keong Tatt foo Department of Urology Singapore General HospitalSingapore

*** Benign prostatic hyperplasia(BPH)is a common urological condition,and yet there are many puzzles waiting to be *** perspective is to help explain some of these puzzles,based on our recent publications and previous... 详细信息

关键词： prostatic BPH hyperplasia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

THE LONG TERM VARIATIONS OF TROPICAL CYCLONE ACTIVITY IN THE SOUTH CHINA SEA AND THE VICINITY OF HONG KONG

引用

Tropical Cyclone Research and Review 2012年第3期1卷 277-292页

作者： TSZ-CHEUNG lee CHRISTY YAN-YU LEUNG MANG-HIN kok HO-SUN CHAN Hong Kong Observatory 134A Nathan RoadKowloonHong KongChina Department of Meteorology School of Mathematical and Physical ScienceUniversity of ReadingWhiteknightsReadingBerkshireRG6 6AHUnited Kingdom

This study examines the long-term variations of tropical cyclone(TC) frequency and intensity in the South China Sea(SCS) and the vicinity of Hong Kong from 1961 to 2010 based on the best track data of four main weather agencies in the western North Pacific, namely the Hong Kong Observatory, China Meteorological Administration, Regional Specialized Meteorological Center Tokyo and Joint Typhoon Warning Center of the United States. To account for the discrepancy in the best track data between agencies, the maximum sustained wind speeds are standardized to the 10-minute average before data analysis. Moreover, a sensitivity assessment based on three different data scenarios is also conducted to study the uncertainty in trend analysis due to the discrepancy in the datasets from various agencies. The results show that, likely modulated by the El Ni?o Southern Oscillation and the Pacific Decadal Oscillation, there exist strong inter-annual and inter-decadal variations in the TC frequency in the SCS and the vicinity of Hong *** the long-term trend, all dataset/scenario combinations depict a decrease in the TC frequency in the SCS and the vicinity of Hong Kong during the study period, but the trend is not statistically significant at 5% level for most of the datasets. As for the TC intensity, the discrepancy between weather agencies remains very substantial even when the difference in the wind speed averaging period is accounted for. The large differences in the available datasets do not allow for a reliable detection of the long-term trend of the TC intensity in the SCS. The study of the TC impacts on Hong Kong reveals that there is no significant trend on the TC-induced extreme rainfall in Hong Kong. The extreme high winds associated with TCs within 500 km range of Hong Kong have no significant trend at Waglan Island(offshore island) while those of the urban station at Kai Tak have a signifi-cant decreasing trend.

关键词： tropical cyclones climate change South China Sea Hong Kong

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Thymosin β4 promotes angiogenesis and increases muscle progenitor cell density in ischemic skeletal muscle in a mouse model of hind limb ischemia

引用

Open Journal of Regenerative Medicine 2012年第2期1卷 19-24页

作者： Yue Zhou Eliana C. Martinez Li-Ping Su kok-Onn lee Lei Ye Department of Medicine National University of Singapore Singapore Minnesota USA Department of Medicine National University of Singapore Singapore Minnesota USA. Department of Medicine University of Minnesota Minnesota USA Department of Surgery National University of Singapore Singapore Minnesota USA Division of Bioengineering National University of Singapore Singapore Minnesota USA

Aim: To determine the therapeutic effect of thy- mosin β4 (Tβ4) for treatment of ischemic limb disease in a mouse model. Methods: A mouse model of hindlimb ischemia was created by permanent ligation of femoral arteries and internal iliac artery. Tβ4 was dissolved in sterile saline and intramuscularly injected into the centre and periphery of ligation area in the treatment group (n = 10) starting from the surgery day until 4 weeks after surgery, while control animals received saline injection only (n = 9). All animals were sacrificed at 6 weeks after surgery and used for immunohistochemistry studies. Results: Tβ4 stimulated angiogenesis was evidenced by increased vascular density based on CD31 immunostaining, which was sig- nifycantly increased in Tβ4 group (562.5 ± 78.4/mm2) as compared with control group (371.1 ± 125.7/mm2;p 0.05) groups. Tβ4 increased Pax3/7+ skeletal muscle progenitor cell density. Pax3/7+ cell density of Tβ4 group (13.7% ± 2%) was significantly higher than that of the control group (4.3% ± 1.6%, p < 0.05). However, the numbers of central nuclei fiber and central nuclei per fiber were insignificantly increased in Tβ4 group as compared to control group. The numbers of central nuclei fiber were 8.9 ± 2.1 and 9.5 ± 1.6, and the central nuclei per fiber were 0.25 ± 0.07 and 0.48 ± 0.09 for control and Tβ4 groups, respectively. Conclusions: This preliminary study suggests that localized delivery of Tβ4 increased angiogenesis and skeletal muscle progenitor cell density in ischemic skeletal muscle, but failed to promote skeletal muscle regeneration.

关键词： Thymosin β4 Neovascularization Progenitor Cell Muscle Regeneration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

子二代中华鲟分枝杆菌感染及血液生理生化指标的变化

引用

中国水产科学 2017年第1期24卷 136-145页

作者：张书环聂品舒少武杜浩 foo Khong lee 杨月梅邵纯邸军黄君曾令兵危起伟农业部淡水生物多样性保护重点实验室中国水产科学研究院长江水产研究所淡水生态与生物技术国家重点实验室中国科学院水生生物研究所香港海洋公园

鱼类分枝杆菌病是由分枝杆菌属的非结核分枝杆菌造成的长期性感染疾病。本研究发现,养殖中华鲟(Acipenser sinensis)子二代可感染该病,典型病理特征是腹水、腹膜肉芽肿、肾脏水肿、肝脏呈灰白色等。采集腹水,通过细菌分离培养获得一株... 详细信息

鱼类分枝杆菌病是由分枝杆菌属的非结核分枝杆菌造成的长期性感染疾病。本研究发现,养殖中华鲟(Acipenser sinensis)子二代可感染该病,典型病理特征是腹水、腹膜肉芽肿、肾脏水肿、肝脏呈灰白色等。采集腹水,通过细菌分离培养获得一株缓慢生长型的抗酸杆菌,进一步通过16S r DNA和IS2404重复序列进行分子生物学鉴定,发现该菌与Mycobacterium liflandii相似性最高。将10尾健康子二代中华鲟3次体检的血液参数与4尾分枝杆菌感染子二代中华鲟12次检查的血液参数进行比较,发现在26项生理生化指标中,有9项指标差异显著(P<0.05),即中性粒细胞、淋巴细胞、钠、氯、钙、总蛋白、球蛋白、外周血白细胞总数(TWBC)和谷丙转氨酶(ALT),其中TWBC、中性粒细胞和ALT这3项指标显著升高,其他指标显著下降,说明分枝杆菌感染对鱼体肝脏和肾脏的损伤较大。采用盐酸卡纳霉素、红霉素和利福平三种抗生素联合用药进行治疗,给药时间75 d,治疗后腹水明显减少,RBC、血红蛋白、PCV、MCV和球蛋白这5项血液理化指标均有显著性升高(P<0.05),说明药物治疗初期具有一定的效果,但随着病程的加剧,该尾鱼最终未能治愈。以上结果表明,分枝杆菌在中华鲟广泛流行,对中华鲟的危害较大,目前尚未见有效的疫苗和药物,因此对该病的预防、早期诊断和治疗极其重要。

关键词：中华鲟人工养殖分枝杆菌血液生理生化指标药物治疗

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Hiddleston因果模型语义学以及前进式与回溯式反事实条件句的区别（英文）

引用

逻辑学研究 2017年第1期10卷 79-94页

作者：李国扬国立中正大学哲学系

某些案例显示反事实条件句(counterfactual conditionals)是有"内在歧义的"(inherently ambiguous),即同一句反事实条件句既可以表达"前进式反事实条件句"(forward-tracking counterfactuals)也可以表达"回溯式反事实条件句"(backtracking counterfactuals)。在之前的文章中(lee 2015,lee 2016),我提出一个因果模型反事实条件句语义学(causal modeling semantics of counterfactuals),主张反事实条件句的内在歧义性是由不同的因果操弄(causal manipulation)所产生的。在一篇很重要的论文中(Hiddleston 2005),Eric Hiddleston提出一个截然不同的因果模型反事实条件句语义学,并宣称这个语义学可以解释反事实条件句的内在歧义性。本文将介绍上述两个因果模型反事实条件句语义学,并试图论证本人的语义学比Hiddleston的语义学能够更好地处理反事实条件句的内在歧义性。

关键词：反事实条件句因果模型语义学回溯歧义性 Eric 主张

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

内皮-氧化氮合酶基因多态性与糖尿病肾病关系的研究

引用

中华检验医学杂志 2004年第5期27卷 303-306页

作者：廖琳赵家军 Lim Min Chen Chan Shzu-Wei lee kok Onn 山东大学山东省立医院内分泌科济南250021 新加坡国立大学医学院内分泌科

目的探讨内皮一氧化氮合酶(eNOS)基因内含子4的多态性与糖尿病肾病的关系,以及其在中国人、马来西亚人和印度人中的分布。方法选择258例病程在10年以上的2型糖尿病患者作为观察对象,其中中国人150例、马来西亚人71例、印度人37例。从... 详细信息

目的探讨内皮一氧化氮合酶(eNOS)基因内含子4的多态性与糖尿病肾病的关系,以及其在中国人、马来西亚人和印度人中的分布。方法选择258例病程在10年以上的2型糖尿病患者作为观察对象,其中中国人150例、马来西亚人71例、印度人37例。从患者全血中提取DNA,然后,进行聚酶链反应(PCR)、克隆及测序。结果以往认为该基因有2种等位基因(a和b),而本研究发现3种等位基因,并对第3种多态(等位基因c)基因进行了序列分析;统计分析显示,等位基因a、b和c均与糖尿病肾病无显著相关性。结论 eNOS基因内含子4上的多态性有3种等位基因;该基因多态性与糖尿病肾病无显著相关性。

关键词：内皮一氧化氮合酶基因多态性糖尿病肾病

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Critical evaluation of contemporary management in a new Pelvic Exenteration Unit: The first 25 consecutive cases

引用

World Journal of Gastrointestinal Oncology 2017年第5期9卷 218-227页

作者： Min Hoe Chew Yu-Ting Yeh Ee-Lin Toh Stephen Aditya Sumarli Ghee Kheng Chew Lui Shiong lee Mann Hong Tan Tiffany Priyanthi Hennedige Shin Yi Ng Say Kiat lee Tze Tec Chong Hairil Rizal Abdullah Terence Lin Hon Goh Mohamed Zulfikar Rasheed kok Chai Tan Choong Leong Tang Department of Colorectal Surgery Singapore General Hospital Department of Obstetrics and Gynaecology Singapore General Hospital Department of Urology Singapore General Hospital Department of Orthopaedic Surgery Singapore General Hospital Division of Oncological Imaging National Cancer Centre Singapore Department of Anaesthesiology Singapore General Hospital Vascular Surgery Singapore General Hospital Department of Plastic Reconstructive and Aesthetic SurgerySingapore General Hospital

To critically appraise short-term outcomes in patients treated in a new Pelvic Exenteration (PE) Unit. METHODSThis retrospective observational study was conducted by analysing prospectively collected data for the first 25 patients (16 males, 9 females) who underwent PE for advanced pelvic tumours in our PE Unit between January 2012 and October 2016. Data evaluated included age, co-morbidities, American Society of Anesthesiologists (ASA) score, Eastern Cooperative Oncology Group (ECOG) status, preoperative adjuvant treatment, intra-operative blood loss, procedural duration, perioperative adverse event, lengths of intensive care unit (ICU) stay and hospital stay, and oncological outcome. Quantitative data were summarized as percentage or median and range, and statistically assessed by the χ2 test or Fisher’s exact test, as applicable. RESULTSAll 25 patients received comprehensive preoperative assessment via our dedicated multidisciplinary team approach. Long-course neoadjuvant chemoradiotherapy was provided, if indicated. The median age of the patients was 61.9-year-old. The median ASA and ECOG scores were 2 and 0, respectively. The indications for PE were locally invasive rectal adenocarcinoma (n = 13), advanced colonic adenocarcinoma (n = 5), recurrent cervical carcinoma (n = 3) and malignant sacral chordoma (n = 3). The procedures comprised 10 total PEs, 4 anterior PEs, 7 posterior PEs and 4 isolated lateral PEs. The median follow-up period was 17.6 mo. The median operative time was 11.5 h. The median volume of blood loss was 3306 mL, and the median volume of red cell transfusion was 1475 mL. The median lengths of ICU stay and of hospital stay were 1 d and 21 d, respectively. There was no case of mortality related to surgery. There were a total of 20 surgical morbidities, which occurred in 12 patients. The majority of the complications were grade 2 Clavien-Dindo. Only 2 patients experienced grade 3 Clavien-Dindo complications, and both required procedura

关键词： Colorectal cancer Advanced pelvic tumour Sacrectomy Oncological outcome Pelvic exenteration Chordoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Entamoeba histolytica acetyl-CoA synthetase:biomarker of acute amoebic liver abscess

引用

Asian Pacific Journal of Tropical Biomedicine 2014年第6期4卷 446-450页

作者： Lim Boon Huat Alfonso Olivos Garcia Tan Zi Ning Wong Weng Kin Rahmah Noordin Siti shafiqah Anaqi Azham lee zhi Jie Guee Cher Ching foo Phiaw Chong Pim Chau Dam Biomedicine Programme.School of Health Sciences Universiti Sains Malaysia Departmento de Medicina Experimental Facultad de MedicinaUniversidad Nacional Autonoma de Mexico Department of Medicine Allianze University College of Medical Sciences Institute for Research in Molecular Medicine 11800 University Sains Malaysia

Objective:To characterize the Entamoeba histolytica(***)antigen(s)recognized by moribound amoebic liver abscess ***:Crude soluble antigen of *** was probed with sera of moribund hamsters in 1D-and 2D-Westem blot *** antigenic protein was then sent for tandem mass spectrometry *** corresponding gene was cloned and expressed in Escherichia coli BL21-AI to produce the recombinant *** ADP-forming acetyl-CoA synthetase(EhACS)protein.A customised ELISA was developed to evaluate the sensitivity and specificity of the recombinant ***:A^75 kDa protein band with a pl value of 5.91-6.5 was found to be antigenic;and not detected by sera of hamsters in the control *** mass spectrometry analysis revealed the protein to be the 77 kDa *** ADP-forming acetyl-CoA synthetase(EhACS).The customised ELISA results revealed 100%sensitivity and 100%specificity when tested against infected(n=31)and control group hamsters(n=5)serum samples,***:This rinding suggested the significant role of EhACS as a biomarker for moribund hamsters with acute amoebic liver abscess(ALA)*** is deemed pertinent that future studies explore the potential roles of EhACS in better understanding the pathogenesis of ALA;and in the development of vaccine and diagnostic tests to control ALA in human populations.

关键词： Entamoeba histolytica Amoebic liver abscess Biomarker Acetyl-CoA synthetase Recombinant EhACS Western blot

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

内皮一氧化氮合酶基因多态性与高脂血症的关系

引用

山东大学学报（医学版） 2005年第6期43卷 496-499页

作者：廖琳赵家军 LIM Min-chen CHAN Shzu-wei lee kok-onn 山东大学山东省立医院内分泌科山东济南250021 新加坡国立大学医院内科内分泌实验室

目的:探讨内皮一氧化氮合酶基因内含子4(eNOS4)多态性与高脂血症的关系。方法:取患者血清,用电化学发光法、钟点法测定血糖、空腹胰岛素(FINS)、糖化血红蛋白(HbA1C)、总胆固醇(TC)、甘油三脂(TG)。从患者全血中提取DNA,进行多聚酶链反... 详细信息

目的:探讨内皮一氧化氮合酶基因内含子4(eNOS4)多态性与高脂血症的关系。方法:取患者血清,用电化学发光法、钟点法测定血糖、空腹胰岛素(FINS)、糖化血红蛋白(HbA1C)、总胆固醇(TC)、甘油三脂(TG)。从患者全血中提取DNA,进行多聚酶链反应(PCR),对PCR产物行6%聚丙烯酰胺凝胶电泳;部分PCR产物被克隆进载体,选阳性集落,进行核酸测序。结果:对照组eNOS4只有2种等位基因(a和b),而糖尿病患者有3种等位基因(a、b和c),对第3种多态(等位基因c)进行了序列分析,其基因序列较等位基因b多27bp,即:GAAGTCTAGACCTGCTGCGGGGGTGAG。患者组等位基因a携带者血清TG及TC显著增高。结论:糖尿病患者eNOS4上的多态性有3种等位基因即:a、b和c;等位基因a与高脂血症,特别是高TG血症显著相关。

关键词：基因,一氧化氮合酶多态性高脂血症糖尿病

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：