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Simultaneous resection of primary colorectal cancer and its liver metastases: why not?

Hepatobiliary Surgery and Nutrition 2019年第3期8卷 321-323页

作者： Joost Hof koert p.de jong University Medical Center Groningen University of GroningenGroningenThe Netherlands

In patients with colorectal cancer and synchronous liver metastases the issue of performing a simultaneous resection of both the primary tumour and the liver metastases is a matter of much debate,especially among *** study by Howard et *** relevant information and is a nice reflection of general daily practice decision making in the Canadian surgical community(1).

关键词： metastases colorectal al.

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Symmetric periodic Orbits and Uniruled Real Liouville Domains

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Chinese Annals of Mathematics,Series B 2016年第4期37卷 607-624页

作者： Urs FRAUENFELdeR Otto van koert department of Mathematics University of Augsburg department of Mathematics and Research Institute of Mathematics Seoul National University

A real Liouville domain is a Liouville domain with an exact anti-symplectic involution. The authors call a real Liouville domain uniruled if there exists an invariant finite energy plane through every real point. Asymptotically, an invariant finite energy plane converges to a symmetric periodic orbit. In this note, they work out a criterion which guarantees uniruledness for real Liouville domains.

关键词： Symmetric periodic orbits Real symplectic manifolds Real uniruledness

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Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices

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World Journal of Gastroenterology 2009年第11期15卷 1359-1366页

作者： Michael A van Geer koert FD Kuhlmann Conny T Bakker Fibo JW ten Kate Ronald pJ Oude Elferink piter J Bosma Liver Center Academic Medical Center of the University of AmsterdamMeibergdreef 69/711105 BK AmsterdamThe Netherlands department of Surgery Academic Medical Center of the University of AmsterdamMeibergdreef 91105BK AmsterdamThe Netherlands department of pathology Academic Medical Center of the University of AmsterdamMeibergdreef 91105 BK AmsterdamThe Netherlands

AIM： To culture human pancreatic tissue obtained from small resection specimens as a pre-clinical model for examining virus-host interactions. METHODS： Human pancreatic tissue samples （malignant and normal） were obtained from surgical specimens and processed immediately to tissue slices. Tissue slices were cultured ex vivo for 1-6 d in an incubator using 95% 02. Slices were subsequently analyzed for viability and morphology. In addition the slices were incubated with different viral vectors expressing the reporter genes GFp or DsRed. Expression of these reporter genes was measured at 72 h after infection.RESULTS： With the Krumdieck tissue slicer, uniform slices could be generated from pancreatic tissue but only upon embedding the tissue in 3% low melting agarose. Immunohistological examination showed the presence of all pancreatic cell types. pancreatic normal and cancer tissue slices could be cultured for up to 6 d, while retaining viability and a moderate to good morphology. Reporter gene expression indicated that the slices could be infected and transduced efficiently by adenoviral vectors and by adeno associated viral vectors, whereas transduction with lentiviral vectors was limited. For the adenoviral vector, the transduction seemed limited to the peripheral layers of the explants. CONCLUSION： The presented system allows reproducible processing of minimal amounts of pancreatic tissue into slices uniform in size, suitable for pre-clinical evaluation of gene therapy vectors.

关键词： pancreas Adenocarcinoma Tissue slicetechnology Ex vivo Adenovirus

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