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呼吸道阻力增加時對呼吸運動的反射性影響

生理学报 1957年第1期 74-79页

作者：林雅各徐宏達上海第一医学院生理学教研组上海第一医学院生理学教研组上海

呼吸道發生部分阻塞以致增加呼吸氣體流通的阻力時,對呼吸運動就要發生影響,首先反映在氣管内的壓力變化以及氣流速度的相應改變。1928年及以後,Fleisch先後在人體以及犬和羊等動物進行實驗觀察,記錄這些變化。從這些變化中,他得出結論... 详细信息

呼吸道發生部分阻塞以致增加呼吸氣體流通的阻力時,對呼吸運動就要發生影響,首先反映在氣管内的壓力變化以及氣流速度的相應改變。1928年及以後,Fleisch先後在人體以及犬和羊等動物進行實驗觀察,記錄這些變化。從這些變化中,他得出結論,認為這時的呼吸運動發生了適應性反應。在切斷迷走神經之後,這種反應仍然存在,因此,他認為:這反應是呼吸肌本體感受器所引起的反射性反應。1935年Van Niekerk和Braak不同意這種意見,他們在割斷狗的兩側頸迷走神經、交感神經、頸脊髓第三至第七節神經背根以及在頸脊髓第七節以下横切脊髓之後,觀察到當增加呼吸道阻力時,氣管的内壓與氣流適應性變化仍然存在,故認為此反應由於呼吸肌的彈性滯性因素所致。

关键词：呼吸道阻力受器雅各反射性呼吸肌

新的氧化应激标志物与冠状动脉疾病患者的死亡风险相关

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中华高血压杂志 2015年第11期23卷 1079-1079页

作者：刘莉叶鹏 Patel RS Ghasemzadeh N Eapen DJ Sher S Arshad S ko ya Veledar E Samady H Zafari AM Sperling L Vaccarino V Jones DP Quyyumi AA

该文旨在验证新出现的以氨基硫醇为标志物的非自由基介导的氧化应激与临床预后的关系。方法：纳入行冠状动脉造影的1411例患者（平均63岁,男性占66%）,应用高效液相色谱仪定量分析还原的（半胱氨酸和谷胱甘肽）和氧化的（半胱氨酸和谷... 详细信息

该文旨在验证新出现的以氨基硫醇为标志物的非自由基介导的氧化应激与临床预后的关系。方法：纳入行冠状动脉造影的1411例患者（平均63岁,男性占66%）,应用高效液相色谱仪定量分析还原的（半胱氨酸和谷胱甘肽）和氧化的（半胱氨酸和谷胱甘肽二硫化物）氨基硫醇水平。

关键词：氧化应激高效液相色谱仪谷胱甘肽死亡风险炎症因子水平全因死亡率协变量

Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats

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World Journal of Gastroenterology 2007年第42期13卷 5605-5611页

作者： Ho-Lam Chung Grace Gar-Lee Yue Ka-Fai To ya-Lun Su Yu Huang Wing-Hung ko Department of Physiology Faculty of MedicineThe Chinese University of Hong Kong Shatin Hong Kong China Institute of Chinese Medicine The ChineseUniversity of Hong Kong Shatin Hong Kong China Department of Anatomical and Cellular Pathology Faculty of Medicine The Chinese University of Hong KongShatin Hong Kong China Institute of Materia Medica Chinese Academy ofMedical Sciences and Peking Union Medical College Beijing100050 China Department of Physiology and LiKa Shing Institute of Health Sciences Faculty of Medicine TheChinese University of Hong Kong Shatin Hong Kong China

AIM： To investigate the effect of Scutellariae Radix extract （SRE） on ulcerative colitis （UC） in rats induced by dextran-sulfate sodium （DSS）. METHODS： Colitis was induced in male Sprague-Dawley （SD） rats （170-180 g） by 4% dextran sulfate sodium （DSS, wt/v, MW 54000） in drinking water for 8 d. The treated rats received 4% DSS and SRE orally （100 mg/kg per day）. Control rats received either tap water or SRE only. Macroscopic assessment which included body weight changes, fecal occult blood and stool consistency were determined daily. At the appointed time, the rats were sacrificed and the entire colons were removed. The colon length and the myeloperoxidase （MPO） activity were measured. The severity of colitis was graded by morphological and histological assessments. The ion transport activity of the colonic mucosa was assessed by electrophysiological technique. RESULTS： Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis. Treatment with SRE relieved the symptoms, including the reduction in body weight, shortening 2nd ulceration of the colon. Administration of SRE also significantly reduced the histological damage induced by DSS. Moreover, the Isc responses of the colonic mucosa to forskolin, were suppressed after the induction of colitis. The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness. CONCLUSION： SRE was effective in treating acute DSS- induced ulcerative colitis, as gauged by reduced clinical disease, improved macroscopic and histological damage scores, and enhanced recovery of normal colonic secretory function.

关键词： Ulcerative colitis Scutellariae Radix Inflammatory bowel disease Colonic ion transport Traditional Chinese medicine

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端粒缩短,再生能力与心血管预后

中华高血压杂志 2016年第12期24卷 1174-1174页

作者：刘莉叶鹏 Hammadah M Mheid IA Wilmot K Ramadan R Abdulhadi N Alkhoder A Obideen M Pimple P Levantsevych O Kelli H Shah A Sun Y Pearce B Kutner M Long Q Ward L ko ya Mohammed KH Lin J Zhao J Bremner J Kim J Waller E Raggi P Sheps D Quyyumi A Vaccarino V

白细胞端粒长度（leucocyte telomere length,LTL）是衰老的生物标志物,较短的LTL与不良的心血管预后相关。端粒再生能力降低可能是其机制之一。骨髓来源的循环祖细胞（progenitor cells,PCs）参与组织修复和再生。该研究的目的是研究LT... 详细信息

白细胞端粒长度（leucocyte telomere length,LTL）是衰老的生物标志物,较短的LTL与不良的心血管预后相关。端粒再生能力降低可能是其机制之一。骨髓来源的循环祖细胞（progenitor cells,PCs）参与组织修复和再生。该研究的目的是研究LTL与PCs之间的关系及其对不良心血管预后的影响。方法：通过定量聚合酶链反应测定566例冠状动脉疾病（coronary artery disease, CAD）门诊患者[年龄（63±9）岁，76％为男性]的LTL。

关键词：端粒 telomere leucocyte 骨髓来源 progenitor 生物标志物祖细胞组织修复 length 四分位数

有氧运动对CNPY2调控NF-κB信号通路改善非酒精性脂肪肝的作用研究

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现代预防医学 2024年第11期51卷 2100-2107页

作者：蒋昌君王佳倩李亚龙李军汉西南石油大学体育学院成都体育学院运动医学与健康学院

目的探讨冠层同源物2(canopy homolog 2,CNPY2)和有氧运动经核因子κB (nuclear factor kappa-B, NF-κB)信号通路在高脂膳食诱导非酒精性脂肪肝(non-alcoholic fatty liver disease, NAFLD)中的作用机制。方法 (12±1)周龄雄性CNPY2基因敲除(CNPY2 ko)小鼠及同系同窝同龄野生(WT)小鼠经一周适应性喂养后随机分为对照组(CON)，高脂模型组(MOD)和高脂模型运动组(MOD+EX)。CON组喂养普通饲料，MOD组和MOD+EX组喂养高脂饲料，直至18周实验结束。从第10周开始，MOD+EX组小鼠进行一周的适应性跑台训练，随后持续运动干预至18周实验结束。全自动生化分析仪检测血清TC、TG、LDL-C、HDL-C、ALT和AST水平；HE染色和油红O染色分析肝脏病理形态；Western Blot检测肝脏CNPY2、IκBα、p-IκBα、NF-κB、p-NF-κB蛋白表达，ELISA检测肝脏炎症因子TNF-α和IL-6水平；qRT-PCR检测肝脏NF-κB mRNA、TNF-α mRNA和IL-6 mRNA表达。通过单因素方差分析和独立样本t检验分析数据。结果 WT小鼠MOD组CNPY2表达较CON组升高(t=-5.730, P=0.001),MOD+EX组CNPY2表达较MOD组降低(t=3.714, P=0.010)；与CON组相比，WT小鼠和CNPY2 ko小鼠MOD组血清TC、TG、LDL-C、ALT、AST水平均升高(WT:t=-13.325、-4.889、-10.442、-3.500、-15.122, P ko:t=-6.910、-4.962、-7.457、-4.584、-7.336, P均ko:t=-18.878、-21.840、-14.033、-12.511、-6.870、-9.546、-8.303, P均ko:t=6.598, Pko小鼠MOD+EX组上述指标均有效改善。与WT小鼠相比，CNPY2 ko小鼠上述指标基本均有效改善。结论 CNPY2调控NF-κB信号通路参与NAFLD的形成与发展。CNPY2基因缺失和有氧运动均能改善NAFLD，其机制可能与有氧运动降低肝脏CNPY2表达、抑制NF-κB信号通路、下调肝脏炎症因子表达水平、减轻肝脏炎症有关。

关键词：有氧运动非酒精性脂肪肝 CNPY2 炎症 NF-κB

Activity of the Mechanically Isolated Hybrid Stromal Vascular Fraction on Osteoarthritis

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International Journal of Clinical Medicine 2023年第11期14卷 505-524页

作者： Polen koçak Serli Canikyan yağiz Denizci Burak Beksaç yaren Kul Tunç Tiryaki Biomedical Engineering Istinye University Istanbul Turkey Genetics and Bioengineering Yeditepe University Istanbul Turkey Faculty of Medicine Medipol University Istanbul Turkey Private Practise Clinic Istanbul Turkey Bioengineering Yildiz Technical University Istanbul Turkey Cadogan Clinic London UK

Background: Regenerative medicine holds promise for treating degenerative and inflammatory conditions like osteoarthritis (OA). However, the complex molecular mechanisms of OA and the limitations of current therapies remain challenges. Adipose-derived stem cells (ADSC) and stromal vascular fraction (SVF) are gaining attention for OA treatment due to their abundance in adipose tissue. The literature delineates two primary techniques for SVF extraction from adipose tissue: enzymatic digestion and mechanical methods. The Lipocube Hybrid SVF presents a straightforward and secure mechanical isolation method for SVF, enhancing its regenerative potential for various applications. Purpose: This study aims to provide valuable insights into the potential of Lipocube Hybrid SVF as a regenerative therapy for OA, contributing to the broader understanding of its applicability in addressing this debilitating condition. Method: To assess the effectiveness and safety of the Lipocube Hybrid SVF, we have designed a comparative study that evaluates cellular activity and viability, phenotypic characterization, and differentiation potential. The in vitro activity of mechanically isolated SVF is compared to the established gold standard enzymatic digestion method. After in vitro studies, Lipocube Hybrid mechanical isolation method was used to isolate SVF and applied in 42 knee and 7 hip joints of 28 patients with Grade II, Grade III, and Grade IV OA. Results: The Lipocube Hybrid group had slightly lower viable cell numbers but higher cell viability. Flow cytometry analysis showed the Lipocube Hybrid group exhibited more favorable markers for regenerative potential and reduced inflammatory response. Additionally, both groups demonstrated successful osteogenic differentiation, with the Lipocube Hybrid group excelling in chondrogenic and adipogenic differentiation. The clinical application of the Lipocube Hybrid SVF in OA patients resulted in significant improvements in WOMAC and VAS scores

关键词： Osteoarthritis Stromal Vascular Fraction Enzymatic Isolation Mechanical Isolation Regenerative Medicine

基于CRISPR/Cas9技术构建PAX3-FOXO1融合基因敲除腺泡状横纹肌肉瘤细胞模型

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石河子大学学报（自然科学版） 2024年第5期42卷 621-630页

作者：张乾儒田娇孟莲史奇李亚夏慧欣李锋石河子大学医学院病理系新疆石河子832000 石河子大学第一附属医院病理科新疆石河子832000 首都医科大学附属北京朝阳医院医学研究中心北京100020

目的使用CRISPR/Cas9技术构建稳定敲除PAX3-FOXO1融合基因的人腺泡状横纹肌肉瘤(ARMS)细胞系(RH30),并验证PAX3-FOXO1功能。方法运用在线网站针对PAX3、FOXO1设计sgRNA,选择切割效率最高的一组构建LV-PAX3-sgRNA、LV-FOXO1-sgRNA及LV-Cas9慢病毒载体;LV-Cas9病毒感染RH30细胞,潮霉素筛选后,通过RT-PCR及qRT-PCR检测RH30-Cas9细胞中Cas9基因表达;LV-PAX3-sgRNA及LV-FOXO1-sgRNA慢病毒载体分别感染RH30-Cas9细胞,流式分选筛选荧光阳性细胞;Sanger测序、PCR等分别验证RH30-PAX3-ko细胞及RH30-FOXO1-ko细胞中PAX3-FOXO1表达;EDU、TUNEL、Transwell实验检测敲除RH30-PAX3-ko细胞及RH30-FOXO1-ko细胞功能改变。结果成功构建靶向PAX3、FOXO1的CRISPR/Cas9慢病毒载体;PCR验证稳定表达Cas9的RH30细胞系(RH30-Cas9)构建成功;PCR、Western Blot验证靶向PAX3或FOXO1均成功敲除RH30中PAX3-FOXO1融合基因;靶向PAX3或FOXO1抑制RH30细胞增殖、侵袭、迁移,促进凋亡。结论使用CRISPR/Cas9成功构建敲除PAX3-FOXO1的RH30细胞系;敲除PAX3-FOXO1抑制了RH30细胞增殖、迁移等恶性生物学行为。

关键词：横纹肌肉瘤 PAX3-FOXO1 CRISPR/Cas9 基因编辑

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Phenotypic research on senile osteoporosis caused by SIRT6 deficiency

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International Journal of Oral Science 2016年第2期8卷 84-92页

作者： De-Mao Zhang Di-Xin Cui Ruo-Shi Xu ya-Chuan Zhou Li-Wei Zheng Peng Liu Xue-Dong Zhou State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University

Osteoporosis is a serious public bone metabolic disease. However, the mechanisms underlying bone loss combined with ageing, which is known as senile osteoporosis, remains unknown. Here we show the detailed phenotype of this disease caused by SIRT6 knock out （ko） in mice. To the best of our knowledge, this is the first study to reveal that SIRT6 is expressed in both bone marrow stroma cells and bone-related cells in both mouse and human models, which suggests that SIRT6 is an important regulator in bone metabolism. SIRT6-ko mice exhibit a significant decrease in body weight and remarkable dwarfism. The skeleton of the SIRT6-ko mouse is deficient in cartilage and mineralized bone tissue. Moreover, the osteocalcin concentration in blood is lower, which suggests that bone mass is markedly lost. Besides, the tartrate-resistant acid phosphatase 5b （TRAP5b） concentration is much higher, which suggests that bone resorption is overactive. Both trabecular and cortical bones exhibit severe osteopenia, and the bone mineral density is decreased. Moreover, double-labelling analysis shows that bone formation is much slower. To determine whether SIRT6 directly regulates bone metabolism, we cultured primary bone marrow stromal cells for osteogenesis and osteoclastogenesis separately to avoid indirect interference in vivo responses such as inflammation. Taken together, these results show that SIRT6 can directly regulate osteoblast proliferation and differentiation, resulting in attenuation in mineralization. Furthermore, SIRT6 can directly regulate osteoclast differentiation and results in a higher number of small osteoclasts, which may be related to overactive bone resorption.

关键词： ageing osteoclastogenesis osteogenesis osteoporosis

基于CiteSpace软件分析小儿外科加速康复外科的研究现状、热点与趋势

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广西医学 2024年第9期46卷 1403-1411页

作者：刘珏杉曹学华毛孝容张雯何雅李晓云杨博四川省医学科学院·四川省人民医院(电子科技大学附属医院)儿童医学中心四川省成都市610072 四川省医学科学院·四川省人民医院(电子科技大学附属医院)妇科四川省成都市610072 四川省医学科学院·四川省人民医院(电子科技大学附属医院)护理部四川省成都市610072 四川省医学科学院·四川省人民医院(电子科技大学附属医院)呼吸与重症医学科四川省成都市610072 四川省医学科学院·四川省人民医院(电子科技大学附属医院)骨科四川省成都市610072

目的基于CiteSpace软件探讨小儿外科加速康复外科(ERAS)的研究现状、热点和趋势。方法在Web of Science核心合集、中国知网数据库中检索2013—2023年与小儿外科ERAS相关的文献,采用CiteSpace软件对年度发文量、国家、作者、关键词等进... 详细信息

目的基于CiteSpace软件探讨小儿外科加速康复外科(ERAS)的研究现状、热点和趋势。方法在Web of Science核心合集、中国知网数据库中检索2013—2023年与小儿外科ERAS相关的文献,采用CiteSpace软件对年度发文量、国家、作者、关键词等进行可视化分析。结果共纳入499篇文献,其中英文文献345篇,中文文献154篇。2013—2023年期间,小儿外科ERAS相关文献的年度发文量总体呈上升趋势。共有43个国家发表小儿外科ERAS相关文献,年度发文量排名前5的国家分别是美国(156篇)、中国(67篇)、加拿大(19篇)、法国(16篇)、德国(15篇)和英国(15篇)。英文文献共有572名作者,核心作者28名,发文量共118篇,已形成以Chen Q、Raval MV、Rove ko等核心作者为代表的研究团队,团队间的合作关系较为紧密。中文文献共322名作者,核心作者40名,发文量共87篇,张现伟、吕成杰、唐维兵等为核心作者,核心作者所形成的研究团队之间合作较少。小儿外科ERAS研究领域中,ERAS方案的制定、ERAS在儿童围手术期的应用和管理、ERAS的应用效果评价为当前的研究热点,疼痛管理、饮食护理、质量改进等是未来的研究趋势。结论国内外小儿外科ERAS研究领域呈快速发展趋势,但我国与欧美国家相比还有一定差距,核心作者研究团队间的合作仍有待加强,未来可借鉴国外实践经验,重视ERAS方案的安全性和适用性,加强临床研究和循证实践,落实围手术期的管理和评价,积极探索适合我国国情的小儿外科ERAS实践方案。

关键词：加速康复外科小儿外科 CiteSpace软件研究现状研究热点研究趋势