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Prediction of protein and amino acid composition and digestibility in individual feedstuffs and mixed diets for pigs using near-infrared spectroscopy

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Animal Nutrition 2021年第4期7卷 1242-1252页

作者： Samantha J.Noel Henry J.Høgh Jørgensen knud E.Bach knudsen Department of Animal Science AU FoulumAarhus UniversityDK 8830TjeleDenmark

Knowledge of the amounts and digestibility of amino acids in pig feedstuffs is essential for calculating the appropriate inclusion level in a complete *** chemical analysis and in vivo digestibility trials are time-consuming and costly and cannot be used for routine ***-infrared spectroscopy(NIRS)offers a rapid,cost effective and environmentally friendly method for evaluating *** models were developed using NIRS to predict the content of crude protein and 18 amino acids from a wide range of feedstuffs used in pig production(n=607).The samples ranged from single feed ingredients(containing amino acids from 0.3 to 129.8 g/kg of dry matter)to feed mixtures(containing amino acids from 1.2 to 53.2 g/kg of dry matter).The predictive ability of the calibrations was tested with an independent dataset(n=150)and with ***,we compare these calibrations with calibrations developed on more narrowly defined groups of samples and with predictions from regression analysis of crude *** models were able to predict the concentrations of crude protein and 18 amino acids with good levels of precision and high coefficients of determination for calibration(RSQ^(CAL))from 0.91 to 0.99 and validation(RSQ^(VAL))from 0.87 to *** models were able to predict all amino acids except tryptophan and valine with greater accuracy than those from protein *** also developed calibration models to predict the apparent ileal and total tract digestibility of protein and amino *** the exception of tryptophan,RSQ values(>0.7)and standard error of cross validation(SECV)values(<5%)were obtained for the digestibility of most of the amino *** conclusion,NIRS can be used to predict crude protein and amino acid concentrations from a wide range of single ingredients and feed mixtures used for pig diets without separate models for each *** digestibility of protein and amino acids can be predicted with an

关键词： Near-infrared spectroscopy Amino acids Protein Apparent ileal digestibility Total tract digestibility Swine

Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs

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Journal of Animal Science and Biotechnology 2017年第1期8卷 134-142页

作者： Hanna Katharina Spindler Rainer Mosenthin Pia Rosenfelder Henry J?rgensen knud Erik Bach knudsen Meike Eklund University of Hohenheim Institute of Animal Science Aarhus University Department of Animal ScienceResearch Center Foulum

Background： Basal ileal endogenous amino acid（AA） losses（IAAend） and standardized ileal digestibility（SID） values of cereal grains, such as barley, are apparently underestimated when determined according to the nitrogen（N）-free method. Regression analysis between the dietary apparent ileal digestible content（c AID） and total crude protein（CP） and AA can be considered as alternative approach to obtain more accurate values for IAAendand SID of AA in cereal ***： Eight hulled barley genotypes were used, with barley being the only source of CP and AA in the assay diets. The diets contained 95 % as-fed of these eight barley genotypes each, ranging in CP content between 109.1 and 123.8 g/kg dry matter（DM）. Nine ileally T-cannulated barrows, average body weight（BW） 30 ± 2 kg, were allotted to a row-column design comprising eight periods with 6 d each and nine pigs. On d 5 and the night of d 6 of every period, ileal digesta were collected for a total of 12 h. The IAAend and the SID were determined by linear regression analysis between c AID and total dietary CP and ***： There exist linear relationships between cA ID and total CP and AA（P 〈 0.001）. The IAAend of CP, Lys, Met, Thr and Trp amounted to 35.34, 1.08, 0.25, 1.02 and 0.38 g/kg DM intake（DMI）, respectively, which are greater compared to average IAAend determined previously under N-free feeding conditions. The SID of CP, Lys, Met, Thr and Trp was 90,79, 85, 79 and 86 %, respectively, and was greater when compared to tabulated values. Moreover, these SID values were greater than those reported in literature, based on correction of apparent ileal digestibility（AID） of CP and AA for their IAAendvalues. Summarized, the results of the present regression analysis indicate greater IAAendin barley-based diets compared to those obtained by N-free ***： For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAAenddetermined und

关键词： Amino acid Barley Basal ileal endogenous loss Growing pigs Regression analysis Standardized ileal digestibility

Quantifying and sorting of gold nanoparticle dimers from complex reaction mixtures using flow cytometry

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Nano Research 2016年第10期9卷 3093-3098页

作者： Jens B. Simonsen Nini E. A. Reeler Anna Fossum knud A. Lerstrup Bo W. Laursen Kasper Nergaard Universitetsparken 5 Nano-Science Center & Department of Chemistry University of Copenhagen Copenhagen DK-2100 Denmark Niels Jensens Vej 2 Sino-Danish Centre for Education and Research (SDC) Aarhus DK-8000 Denmark Ole Maaloes Vej 5 Biotech Research and Innovation Centre (BRIC) University of Copenhagen Copenhagen DK-2200 Denmark

The formation, characterization, and purification of well-defined stoichiometric clusters of metallic nanoparticles, particularly in the form of dimers or trimers, are important and formidable challenges in nanoscience. Here we show that flow cytometry can be used as a high-throughput method to determine the relative distribution of oligomeric clusters of molecularly linked gold nano- particles in bulk solution at the single-particle level with good statistics. This unique information would be near impossible to obtain using traditional characterization techniques. The flow cytometric approach is utilized to provide fast feedback for the synthesis optimization of the complex reaction between citrate-stabilized gold nanoparticles and bi-functional molecular wires with dithiocarbamate anchoring groups. Finally, we demonstrate that flow cytometry can be used to significantly increase the proportion of AuNP dimers from an oligomer-rich polydisperse sample by size-selective sorting.

关键词： flow cytometry characterization sorting nanoparticles gold nanoparticle dimers

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北京华商工业园

建筑学报 2004年第3期 66-69页

作者： knud Rossen 维思平建筑设计咨询有限公司北京100044

该工业园的设计导入了酒店式工厂的概念,用庭院、水景,绿化等手段塑造了全新的工业建筑形象。

关键词：北京华商工业园酒店式工厂庭院水景绿化

西方的“1968”：学生运动的起源、过程和后果

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史林 2012年第5期 160-165页

作者： knud Andresen[德] 吕澍汉堡大学当代史研究所

1968这一年在西方工业国的集体记忆里留下了各种的深刻烙印。对保守者来说,这是一个社会与政治价值观坍塌的开始;而对全球左派而言,它带来了社会与政治自由化。学生要求全面的民主,要求结束越南战争和更多的社会自由。"1968"年这一概念... 详细信息

1968这一年在西方工业国的集体记忆里留下了各种的深刻烙印。对保守者来说,这是一个社会与政治价值观坍塌的开始;而对全球左派而言,它带来了社会与政治自由化。学生要求全面的民主,要求结束越南战争和更多的社会自由。"1968"年这一概念下集中了众多因素。美国和联邦德国学生运动的新左派尽管都经历了政治激进化和理论新建的过程,但是其影响力却体现在长期的、首先是新的生活方式之中。"1968"年发生的那些事件,造成了以社会地位的不同为背景的生活方式的个体化和集体化,但也并非其唯一的起因。价值观转变的冲动是青年整体的诉求,要求生活质量和自我决定权而不接受作为规范的工作纪律、道德纯洁和责任义务。尽管新的挑衅及交往战略在学生运动中做了尝试,普遍的个体化和民主化以此得以加速,但并非由此引发。它是1960年代以来西方工业国家的复杂的现代化过程的一部分。

关键词： 1968年青年运动西方工业国价值观改变

Bioabsorbable Barrier Membrane Combined with rhBMP-2 Improved Bone Formation in an Experimental Model of Compromised Healing But Was Not Superior to rhBMP-2 Alone

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