检索结果-南通市图书馆

Journal of Biosciences and Medicines 2015年第3期3卷 50-55页

作者： Masaaki Minami Syun Syun Torii Michio ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

In Streptococcus pyogenes, we have described the two-component signal transduction sensor and regulatory systems, CovR/S affect the antimicrobial susceptibility including penicillin G before. But the mechanism how two-component sensor protein CovS modulates penicillin G susceptibility has not been elucidated till date. This study aimed to determine how the CovS affected penicillin G susceptibility in Streptococcus pyogenes by northern blot analysis. At first, we investigated the covS mRNA expression under penicillin G induction. We found that the decrease of covS mRNA expression under Penicillin G stimulation. Next we investigated the expression of cell wall synthesis gene, pbp2a and glmM with use of covS knockout mutants from emm1 Streptococcus pyogenes strain 1529. We found that the cell-wall synthesis gene expression of the ?covS mutant strain were lower than that of the wild-type strain. Furthermore the expression of glmM mRNA gene was lower than the expression of pbp2a mRNA gene in the ?covS mutant strain. The covS-complemented strain almost restored the mRNA expression compared to covS mutant strain. The two-component sensor protein CovS affects the susceptibility to penicillin G in Streptococcus pyogenes by modulation of cell-wall synthesis.

关键词： Streptococcus Pyogenes CovS pbp2a glmM

EFFECT OF RAIN- AND ICE-INDUCED DEPOLARIZATION ON DUAL POLARIZED 21GHz-BAND SATELLITE BROADCASTING SYSTEM

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

EFFECT OF RAIN- AND ICE-INDUCED DEPOLARIZATION ON DUAL POLAR...

1993 International Symposium on Radio Propagation(ISRP’93)

作者： Hiroki ohta Hajime Fukuchi Communications Research Laboratory M.P.T.Tokyo 184Japan

＜正＞We present relation between attenuation and XPD measured over several years by using a circularly polarized beacon signal at 19.5 GHz transmitted from Japanese geostationary communication satellite *** also present results of computer simulation for bit-error-rate performance with the function of Eb/No ratio and D/U ratio assuming QPSK modulation methods,and co-channel dualpolarized channel *** combining the results mentioned above,effect of depolarization on the system performance of dual polarized 21GHz-band satellite broadcasting is discussed.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

基于动态规划的高速立体影像匹配系统

测绘译丛 1990年第1期 10-18页

作者： ohta,Y 周月琴

A case of mucosa-associated lymphoid tissue lymphoma forming multiple lymphomatous polyposis in the small intestine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第9期13卷 1453-1457页

作者： Naoto Hirata Kazunari Tominaga Kensuke ohta Kaori Kadouchi Hirotoshi Okazaki Tetsuya Tanigawa Masatsugu Shiba Toshio Watanabe Yasuhiro Fujiwara Shiro Nakamura Nobuhide Oshitani Kazuhide Higuchi Tetsuo Arakawa Department of Gastroenterology Osaka City University Graduate School of Medicine 1-4-3 Asahimachi Abeno-ku Osaka 545-8585 Japan Department of Hematology Osaka City University Graduate School of Medicine1-4-3 Asahimachi Abeno-kuOsaka 545-8585 Japan Department of Gastroenterology Rinku General Medical Center 2-3 Rinkuouraikita Izumisano Osaka 598-0048Japan

A 50-year old woman suffering from diabetes had a CT scan that revealed a diffuse thickening of small intestinal wall and swollen paraaortic lymph nodes. An esophago gastroduodenoscopy (EGD) confirmed multiple polypoid lesions in the duodenum and small intestine, and conventional histological testing revealed non-specific inflammatory changes. Further examinations including the immunohistochemical profiles of the biopsied specimens led us to diagnose the lesion as a marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type, forming multiple lymphomatous polyposis sequentially spreading from duodenal bulb to terminal ileum. According to Lugano’s classification, its staging was clinically diagnosed as stage Ⅱ. Two courses of a standard CHOP (cyclophosphamide, doxorubicin hydrochloride, vincristine sulfate, and predonisolone) regimen with rituximab reduced the lesion and the patient had a almost complete response. A 5-year follow- up EGD and histological examinations detected no recurrence of the disease.

关键词： Intestinal lymphoma Hucosa-associated lymphoid tissue lymphoma Hultiple lymphomatous polyposis

DESIGN METHOD OF LEG STRUCTURE OF JACK-UP RIGS

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 1993年第4期5卷 57-62页

作者： T.ohta H.Yamauchi M.Toriumi Engineering Research Center,NKK Corporation,Kawasaki,Japan

The structural failures of jack-up rigs were mostly found in leg structures,due to cracks in way of connections between tubular members of leg ***,stress calculations of leg structures can be made by using“finite element techniques”,it takes a lot of time to complete the calculations for the full cases of all working conditions because of many tubular ***,a simple method for the stress calculations for design purposes is strongly *** paper desribes a simple stress calculaiton method of leg structures and shows a satisfactory results as compared with the results obtained by a detailed calculation method.

关键词： jack-up rigs leg structures simple design method stresses

Tropomyosin Isoform Expression in the Adductor Muscle of the Japanese Pearl Oyster, <i>Pinctada fucata</i>

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

American Journal of Molecular Biology 2019年第1期9卷 16-27页

作者： Daisuke Funabara Ayaka ohta Jungo Sueyoshi Satoshi Kanoh Graduate School of Bioresources Mie University Tsu Japan

We determined the full-length primary structure of the tropomyosin (TM)-1 and -2 proteins from the adductor muscle of the Japanese pearl oyster Pinctada fucata (Pifuc-TM-1 and Pifuc-TM-2), and found that they are each composed of 284 amino acid residues. We predicted the gene structure of P. fucata TM (Pifuc-TM) using Splign alignment of our cDNA with genomic sequences and elucidated that Pifuc-TM consists of 10 exons. Exons 1 - 3 and 5 - 10 are used to transcribe Pifuc-TM-1 mRNA, and exons 1 - 4 and 6 - 10 are used to transcribe Pifuc-TM-2 mRNA. Both genes share the same start and stop codons located in exon 1 and exon 10, respectively. Using quantitative real-time PCR, we determined that the Pifuc-TM-1 gene was mainly expressed in adductor phasic muscle, and at a relatively weaker level in adductor catch muscle, whereas the Pifuc-TM-2 gene was expressed equally in both phasic and catch muscles. They were weakly expressed in gill and mantle. Immunoblot analysis using anti-Pifuc-TM-1 and anti-Pifuc-TM-2 antibodies revealed that adductor phasic muscle contained Pifuc-TM-1, while adductor catch muscle contained both Pifuc-TM-1 and Pifuc-TM-2. Differential scanning calorimetry (DSC) analysis was carried out for Pifuc-TM-1 and Pifuc-TM-2 expressed in bacteria, as well as TM purified from P. fucata phasic and catch muscle tissues (phasic-TM and catch-TM). The DSC data indicated that phasic-TM was mainly composed of Pifuc-TM-1, whereas catch-TM contained Pifuc-TM-1 and Pifuc-TM-2. These findings suggest that the distribution of Pifuc-TM-1 and Pifuc-TM-2 in adductor muscle is specific to the muscle fiber type, and reflects the properties of each.

关键词： Adductor Muscle Alternative RNA Processing Catch Contraction Pearl Oyster Tropomyosin

Role of Cathepsin G in the Degradation of Glyceraldehyde-3-Phosphate Dehydrogenase Triggered by 4-Hydroxy-2-Nonenal in U937 Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CellBio 2014年第2期3卷 35-42页

作者： Satoshi ohta Noriko Suzuki Shigeki Kobayashi Toshiyuki Chikuma Department of Analytical Chemistry of Medicines Showa Pharmaceutical University Tokyo Japan

Degradation of oxidized or oxidatively modified proteins is an essential part of the cellular antioxidant defense system. 4-Hydroxy-2-nonenal (HNE), a major reactive aldehyde formed by lipid peroxidation, causes many types of cellular damage. HNE-modified proteins are degraded by the ubiquitin-proteasome pathway or the lysosomal pathway. However, our previous studies using U937 cells showed that HNE-modified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is degraded by cathepsin G. In the present study, we examined whether GAPDH in U937 cells treated with HNE in culture is degraded similarly to that incubated with HNE and U937 cell extract. Treatment with HNE for 10 min in culture decreased GAPDH activity in a concentration dependent manner, but did not affect GAPDH degradation. The proteasome activities were not affected by HNE, but culturing with HNE decreased cathepsin G activity and protein level in a concentration dependent manner. These results suggest that HNE-induced oxidative stress leads to decreased cathepsin G activity and results in the loss of GAPDH degradation. Taken together, our findings indicate that cathepsin G has an important role in the degradation of oxidatively modified GAPDH in U937 cells.

关键词： 4-Hydroxy-2-Nonenal Glyceraldehyde-3-Phosphate Dehydrogenase Cathepsin G U937 Oxidative Stress Proteasome

Effect of phlorizin on metabolic abnormalities in Spontaneously Diabetic Torii (SDT) rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Animal Sciences 2012年第2期2卷 113-118页

作者： Takeshi ohta Hisayo Morinaga Takuji Yamamoto Takahisa Yamada Laboratory of Animal Breeding and Genetics Graduate School of Agriculture Kyoto University Kyoto Japan Laboratory of Animal Genetics Graduate School of Science and Technology Niigata University Niigata Japan

The Spontaneously Diabetic Torii (SDT) rat is a novel model for nonobese type 2 diabetes. In this study we investigated the glycolipid metabolic changes with phlorizin-treatment, which inhibits intestinal glucose uptake and renal glucose reabsorption, in male SDT rats. Phlorizin (100 mg/kg, b.i.d., s.c.) was administered for 4 weeks to SDT rats from 20 to 24 weeks of age. As a result, phlorizin reduced the development of hyperglycemia and decreased the hemoglobin A1c (HbA1c) levels. In the liver, phlorizin increased mRNA levels of glucokinase, the enzymes related with the glycogen cascade and the proteins associated with lipid metabolism. In conclusion, chronic administration of phlorizin in SDT rats produced a good glycemic control and an improvement in liver function.

关键词： Hyperglycemia Phlorizin SDT Rat

Seismic pounding and collapse behavior of neighboring buildings with different natural periods

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Science 2012年第8期4卷 686-693页

作者： Daigoro Isobe Tokiharu ohta Tomohiro Inoue Fujio Matsueda Collaborative Research Center Ashikaga Institute of Technology Ashikaga-shi Japan Division of Engineering Mechanics and Energy University of Tsukuba Tsukuba-shi Japan Kyoryo Consultants Ltd. Tokyo Japan Science Programs Division Japan Broadcasting Corporation Tokyo Japan

Seismic pounding phenomena, particularly the collision of neighboring buildings under long-period ground motion, are becoming a significant issue in Japan. We focused on a specific apartment structure called the Nuevo Leon buildings in the Tlatelolco district of Mexico City, which consisted of three similar buildings built consecutively with narrow expansion joints between the buildings. Two out of the three buildings collapsed completely in the 1985 Mexican earthquake. Using a finite element code based on the adaptively shifted integration (ASI)-Gauss technique, a seismic pounding analysis is performed on a simulated model of the Nuevo Leon buildings to understand the impact and collapse behavior of structures built near each other. The numerical code used in the analysis provides a higher computational efficiency than the conventional code for this type of problem and enables us to address dynamic behavior with strong nonlinearities, including phenomena such as member fracture and elemental contact. Contact release and recontact algorithms are developed and implemented in the code to understand the complex behaviors of structural members during seismic pounding and the collapse sequence. According to the numerical results, the collision of the buildings may be a result of the difference of natural periods between the neighboring buildings. This difference was detected in similar buildings from the damages caused by previous earthquakes. By setting the natural period of the north building to be 25% longer than the other periods, the ground motion, which hada relatively long period of 2 s, first caused the collision between the north and the center buildings. This collision eventually led to the collapse of the centerbuilding, followed by the destruction of the north building.

关键词： Seismic Pounding Collapse Behavior Neighboring Buildings Natural Period Asi-Gauss Technique

肾及尿路疾患病人尿IL－6的检测及其临床意义

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际免疫学杂志 1994年第1期17卷 52-53页

作者： ohta-K 余英豪

肾及尿路疾患病人尿ＩＬ－６的检测及其临床意义［英］／ＯｈｔａＫ…／／ＣｌｉｎＮｅｐｈｒｏｌ．－１９９２，３８（４）．－１８５～１８９ＩＬ－６在宿主炎症及免疫反应中担负着重要作用。据报道肾小球系膜细胞可产生ＩＬ－６，因... 详细信息

肾及尿路疾患病人尿ＩＬ－６的检测及其临床意义［英］／ＯｈｔａＫ…／／ＣｌｉｎＮｅｐｈｒｏｌ．－１９９２，３８（４）．－１８５～１８９ＩＬ－６在宿主炎症及免疫反应中担负着重要作用。据报道肾小球系膜细胞可产生ＩＬ－６，因而推测ＩＬ－６可能与系膜增生性肾小...

关键词：病人疾患尿路 IL 临床意义