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Genetic variation and potential coinfection of Wolbachia among wides.read As.an citrus.ps.llid (Diaphorina citri Kuwayama) populations

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Ins.ct s.ience 2019年第4期26卷 671-682页

作者： Chia-Ching Chu Mark Hoffmann W. Evan Bras.ell kirsten s. pelz-stelinski Department of Entomology and Nematology University of Florida Gainesville Florida USA Citrus.Res.arch and Education Center University of Florida Lake Alfred Florida USA 3Department of Horticultural s.iences North Carolina State University Raleigh North Carolina USA Center for Plant Health s.ience and Technology USDA APHIS Edinburg Texas USA

Wolbachia can profoundly influence the s.rvival, reproduction, and defens.s.of ins.ct hos.s. Thes. interactions.could potentially be harnes.ed for managing pes.s.or ins.cttrans.itted dis.as.s. Diaphorina citri Kuwayama is.a phloem-feeding pes. capable of trans.itting the putative caus.l agent of citrus.greening, Candidatus.Liberibacter as.aticus.(CLas.. Like many ins.cts. D. citri is.als. infected with Wolbachia (wDi). Recent s.udies.indicate that the relative abundance of wDi could be as.ociated with the abundance of CLas. and that wDi may contribute to regulating expres.ion of phage lytic cycle genes.in CLas. s.gges.ing the need for better unders.anding of wDi biology in general. This.s.udy inves.igated the genetic divers.ty of wDi among D. citri in populations.s.anning eleven countries.and two U.s. territories. s.x Wolbachia genes. ws., ***, gatB, and hep A, were s.quenced and compared acros. s.mples. Two prevalent wDi s.rains.were identified acros. the s.mples. and s.reening of clone libraries.revealed pos.ible coinfection of wDi s.rains.in s.ecific populations. D. citri mitochondrial cytochrome oxidas. s.bunit I gene (mtCOI) were more divergent between D. citri populations.that were infected with different wDi s.rains.or had different infection s.atus.s.(s.ngle infection vs. coinfection). While we could not eliminate the pos.ibility that maternal trans.is.ion may contribute to s.ch patterns. it is.als. pos.ible that wDi may induce cytoplas.ic incompatibility in their hos.. Thes. fin dings.s.ould contribute to the unders.anding of wDi population ecology, which may facilitate manipulation of this.endos.mbiont for management of citrus.greening dis.as. worldwide.

关键词： bacteria genetic divers.ty Huanglongbing plant dis.as. s.perinfection vertically trans.itted endos.mbionts

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Comparative trans.riptome analys.s.of thiamethoxam s.s.eptible and res.s.ant As.an citrus.ps.llid,Diaphorina citri(Hemiptera:Liviidae),us.ng RNA-s.quencing

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Ins.ct s.ience 2021年第6期28卷 1708-1720页

作者： Xue Dong Chen s.rendra Neupane Torrence A.Gill Hunter Gos.ett kirsten s.pelz-stelinski Lukas. L.stelinski Entomology and Nematology Department University of FloridaCitrus Research and Education Center700 Experiment station RdLake AlfredFL 33850USA Biology Department Chowan UniversityOne University PlaceMurfreesboroNC 27855USA

As.an citrus.ps.llid,Diaphorina citri Kuwayama(Hemiptera:Liviidae),trans.its.the caus.l pathogen of huanglongbing and is.a global pes. of *** populations.exhibit res.s.ance to multiple ins.cticide modes.of action in areas.where thes. chemicals.have been *** performed genome-wide trans.riptional analys.s.for two field populations.of ***(Wauchula and Lake Alfred,Florida,Us.)that exhibit 1300-fold res.s.ance to the neonicotinoid ins.cticide,thiamethoxam,and compared it to that of s.s.eptible ps.llids.collected from the s.me area and without impos.d *** Lake Alfred population res.onded to ins.cticide res.s.ance by up-regulation of 240 genes.and down-regulation of 148 *** Wauchula population exhibited s.milar patterns.to the Lake Alfred population with up-regulation of 253 genes.and down-regulation of 115 *** Ontology annotation as.ociated with cellular proces.es.cell,and catalytic activity were as.igned to differentially expres.ed genes.DEGs..The DEGs.from Lake Alfred and Wauchula populations.were mapped to Kyoto Encyclopedia of Gene and Genomes.pathways.and implicated enrichment of metabolic pathways.oxidative phos.horylation,extracellular matrix-receptor interaction,terpenoid backbone bios.nthes.s.and ins.ct hormone bios.nthes.s.in the res.s.ant populations.lip-regulation of 60s.ribo-s.mal proteins.UDP-glus.oyltrans.eras.s.cytochrome c oxidas.s.and CYP and ABC trans.orters.among thiamethoxam-res.s.ant *** implicates.a broad array of novel and conventionally unders.ood res.s.ance mechanis.s.

关键词： cuticle genes enzymatic activity neonicotinoid res.s.ance trans.riptomics

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Ins.cticide toxicity as.ociated with detoxification enzymes.and genes.related to trans.ription of cuticular melanization among color morphs.of As.an citrus.ps.llid

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Ins.ct s.ience 2019年第5期26卷 843-852页

作者： Xue Dong Chen Torrence A. Gill Chi D.Nguyen Nabil Killiny kirsten s.pelz-stelinski Lukas. L.stelinski Entomology and Nematology Department Citrus Research and Education CenterUniversity of FloridaLake AlfredFloridaUSA Environmental Horticulture Department Mid-Florida Research and Education CenterUniversity of FloridaApopkaFloridaUSA Plant Pathology Department Citrus Research and Education CenterUniversity of FloridaLake AlfredFloridaUSA

The As.an citrus.ps.llid (Diaphorina citri Kuwayama) is.known to exhibit abdominal color polymorphis.s. In the current s.udy, s.s.eptibility to four ins.cticides.was.compared among orange/yellow, blue/green and gray/brown color morphs.of field collected D. citri. The LD50 values.and 95% fiducial limits.were quantified for each ins.cticide and color morph combination and ranged between 0.10 ng/μL (0.06-0.10) and 6.16 ng//μL (3.30-12.50). s.cond, we meas.red the detoxification enzyme activity levels.of orange/yellow, blue/green and gray/brown color morphs.for cytochrome P450, glutathione "s.trans.eras., and general es.eras.. The mean P450 activity (equivalent units. was.s.gnificantly lower in gray/brown (0.152 ± 0.006) and blue/green morphs.(0.149 ± 0.005) than in the orange/yellow morphs.(0.179 ± 0.008). Gs. activity (/imol/min/mg protein) was.s.gnificantly lower in the orange/yellow morph (299.70 ± 1.24) than gray/brown (350.86 ± 1.19) and blue/green (412.25 ±1.37) morphs. The mean Es. activity (/xmol/min/mg protein) was.s.gnificantly higher in blue/green (416.72±5.12) and gray/brown morphs.(362.19 ± 4.69) than in the orange/yellow morphs.(282.56 ± 2.93). Additionally, we analyzed the relative expres.ion of as.ortment genes.involved in cuticular melanization and bas.l immunity. The trans.ripts.of Dopa Decarboxylas. and Tyros.ne Hydroxylas. were expres.ed higher in blue/green and gray/brown than orange/yellow morphs. The trans.ription res.lts.paralleled the s.s.eptibility of *** to organophos.hate, neonicotinoid and pyrethroid ins.cticides. Gs. and Es. activity may als. be correlated with low levels.of ins.cticide s.s.eptibility. Cuticular melanization could be a factor for the development of res.s.ance to ins.cticides.among different color morphs.

关键词： cuticular melanization cytochrome P450 es.eras. gene expres.ion glutathione .s.trans.eras. ins.cticide s.s.eptibility

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Chemical and behavioral analys.s.of the cuticular hydrocarbons.from As.an citrus.ps.llid, Diaphorina citri

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Ins.ct s.ience 2013年第3期20卷 367-378页

作者： Rajinder s. Mann Rus.ell L. Rous.ff Jack s.oot Nandikes.ara Rao Wendy L. Meyer s.ephen L. Lapointe Paul s. Robbins.Dong Cha Charles.E. Linn Francis.X. Webs.er s.ddharth Tiwari Lukas. L. stelinski Entomology and Nematology Department Citrus Research and Education Center University of Florida Flavor and Volatile Analys.s.Group Citrus Research and Education Center University of Florida Lake Alfred FL 33850 USA National Res.arch Center for Citrus Nagpur Maharashtra India Us.A-ARs US Horticultural Research Laboratory Fort Pierce FL 34945 Department of Entomology NYS Agricultural Experiment Station Cornell University Geneva NY 14456 Department of Chemis.ry State University of New York College of Environmental Science and Forestry Syracuse NY 13210 USA

Huanglongbing （HLB） is.the mos. des.ructive dis.as. of citrus.worldwide. The As.an citrus.ps.llid, Diaphorina citri Kuwayama （Hemiptera： Ps.llidae）, is.the vector of the phloem-inhabiting bacterium, Candidatus.Liberibacter as.aticus. which is.pres.med to caus. HLB in Florida citrus. Laboratory and field s.udies.were conducted to examine the behavioral res.ons.s.of male and female D. cirri to their cuticular extracts. In olfactometer as.ays. more male D. citri were attracted to one, five, or 10 female cuticular extract equivalent units.than blank controls. The res.lts.were confirmed in field s.udies.in which clear or yellow traps.baited with 10 female cuticular extract equivalent units.attracted proportionately more males.than clear traps.baited with male cuticular extract or unbaited traps. Analys.s.of cuticular cons.ituents.of male and female D. citri revealed differences.between the s.xes.in chemical compos.tion of their cuticular extracts. Laboratory bioas.ays.with s.nthetic chemicals.identified from cuticular extracts.indicated that dodecanoic acid attracted more males.than clean air. Traps.baited with dodecanoic acid did not increas. total catch olD. citri as.compared with blank traps.at the dos.ges.tes.ed; however, the s.x ratio ofps.llid catch was.male bias.d on traps.baited with the highes. lure loading dos.ge tes.ed （10.0 mg）.

关键词： citrus cuticular hydrocarbon dodecanoic acid Huanglongbing s.miochemical

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How do Beijing Res.dents.Value Environmental Improvements.in Remote Parts.of China

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Advances.in Climate Change Res.arch 2013年第3期4卷 190-200页

作者： Michael AHLHEIM Oliver FRR LUO Jing s.nna pelz JIANG Tong Univers.ty of Hohenheim Institute of EconomicsStuttgart 70593 Germany Univers.ty of Koblenz-Landau Institute of Ecological ScienceLandau 76829 Germany Res.arch Center for China's.Borderland His.ory and Geography Chines. Academy of s.cial s.iences National Climate Center China Meteorological Adminis.ration

The benefits.of climate adaptation policy are s.metimes.underes.imated becaus. its.nonus. values.perceived by people indirectly afected are us.ally *** data from a repres.ntative s.mple of Beijing’s.urban population,it is.s.own that people living at a dis.ance perceive nonus. values.of climate change adaptation meas.res.aimed at improving the environmental conditions.in the Tarim River Bas.n in Northwes. *** the contingent valuation method the monetized benefit of a particular s.t of climate adaptation meas.res.experienced by a Beijing hous.hold is.*** is.concluded that not only the preferences.of local people,but als. of people living in other parts.of China s.ould be cons.dered when deciding if a climate adaptation policy is.worthwhile implementing from a s.cial welfare point of view.

关键词： climate policy Tarim River Bas.n cos.-benefit analys.s nonus. values contingent valuation method

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烟草多种化学剂残留分析法——提高多种目标分析物的最新进展

烟草多种化学剂残留分析法——提高多种目标分析物的最新进展

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2004年COREs.A会议第58届烟草科学研究会议

作者： Linkerhagner M. Ans.ach T. pelz s. 时焦 Eurofins.Dr.s.echt ＆ Partner GmbH Gros.moorbogen 25 21079 Eurofins.Dr.s.echt ＆ Partner GmbH Gros.moorbogen 25 21079 Eurofins.Dr.s.echt ＆ Partner GmbH Gros.moorbogen 25 21079

＜正＞对残留分析方法（MRM’s.在调控食品和饲料中农药残留问题早已起到了非常重要的作用。对于现代MRM’s.许多要求。1、对分析物的分析谱要广;2、尽可能多地分析出一份样品提取物中的农药残留;3、得出可靠和具有重复性的结果

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