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Binary Vector Construction for site-Directed Mutagenesis of <i>Kafirin</i>Genes in sorghum

American Journal of Plant sciences 2021年第8期12卷 1276-1287页

作者： Grigoriy A. Gerashchenkov Lev A. Elkonin kirill G. Gerashchenkov Natalia A. Rozhnova stefan Hiekel Jochen Kumlehn Alexey V. Chemeris Institute of Biochemistry and Genetics Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences Ufa Russia Federal Centre of Agriculture Research of south-East Region Saratov Russia Kazan Federal University Institute of Fundamental Medicine and Biology Kazan Russia Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben Germany

sorghum (sorghum bicolor (L.) Moench) is one of the world’s leading cereal crops in agricultural production, which has a special importance in the arid regions. However, unlike other cereals, sorghum grain has a lower nutritional value, which is caused, inter alia, by the resistance of its seed storage proteins (kafirins) to protease digestion. One of the effective approaches to improve the nutritional value of sorghum grain is to obtain mutants with partially or completely suppressed synthesis or altered amino acid composition of kafirins. The employment of genome editing may allow to solve this problem by introducing mutations into the nucleotide sequences of the α- and γ-kafirin genes. In this study, genomic target motifs (23 bp sequences) were selected for the introduction of mutations into the α- and γ-KAFIRIN genes of sorghum. The design of the gRNAs was conducted using the online tools CRIsPROR and CHOPCHOP. Two most suitable targets were chosen for α-KAFIRIN (k1C5) and two for γ-KAFIRIN (gKAF1) genes. The insertion of respective sequences in the generic vector psH121 was performed at the BsaI (Eco31I) sites. Validation of the cloning procedure was performed by DNA sequencing. subcloning of the resulting constructs was performed using the sfiI restriction sites into the compatible binary vector B479p7oUZm-LH. The correct assembly of binary vectors was confirmed by restriction analysis using the MluI and sfiI cleavage sites. The four vectors created (1Cspan> - 4C) were transferred by electroporation into the Agrobacterium tumefaciens strain AGL0. Currently, this vector series is used for stable

关键词： sorghum bicolor (L.) Moench CRIsPR/Cas Genome Editing α-Kafirin γ-Kafirin Genetic Engineering Grain Quality

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Isolation and Characterization of a New Cyanobacterial strain with a Unique Fatty Acid Composition

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Advances in Microbiology 2014年第15期4卷 1033-1043页

作者： Fariza K. sarsekeyeva Aizhan A. Usserbaeva Bolatkhan K. Zayadan kirill s. Mironov Roman A. sidorov Anna Yu. Kozlova Elena V. Kupriyanova Maria A. sinetova Dmitry A. Los Institute of Plant Physiology Russian Academy of Sciences Moscow Russia Department of Biotechnology Faculty of Biology and Biotechnology Al-Farabi Kazakh National University Almaty Kazakhstan

A new cyanobacterial strain was isolated and purified from salt Lake Balkhash, Kazakhstan. According to its morphological and ultrastructural characteristics, 16s rRNA sequence and the fatty acid profile, the strain has been classified as Cyanobacterium spp. and assigned as Cyanobacterium sp. IPPAs B-1200. The strain is characterized by a non-temperature inducible Δ9-desaturation system, and by high relative amounts of myristic (14:0—30%) and myristoleic (14:1Δ9—10%) acids. The total amount of C14 fatty acids reaches 40%, which is unusually high for cyanobacteria, and it has never been reported before. The remaining fatty acids are represented mainly by palmitic (16:0) and palmitoleic (16:1Δ9) acids (the sum reaches nearly 60%). such a fatty acid composition, together with a relatively high speed of growth, makes this newly isolated strain a prospective candidate for biodiesel production.

关键词： Cyanobacterium Fatty Acids Lake Balkhash Myristic Acid Myristoleic Acid 16s rRNA

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Research on the knee region of cosmic ray by using a novel type of electron-neutron detector array

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Frontiers of physics 2024年第4期19卷 13-24页

作者： Bing-Bing Li Xin-Hua Ma shu-Wang Cui Hao-Kun Chen Tian-Lu Chen Danzengluobu Wei Gao Hai-Bing Hu Denis Kuleshov kirill Kurinov Hu Liu Mao-Yuan Liu Ye Liu Da-Yu Peng Yao-Hui Qi Oleg shchegolev Yuri stenkin Li-Qiao Yin Heng-Yu Zhang Liang-Wei Zhang College of Physics Hebei Normal UniversityShijiazhuang 050024China Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China TIANFU Cosmic Ray Research Center Chengdu 610000China College of science Tibet UniversityLhasa 850000China Key Laboratory of Comic Rays Ministry of EducationTibet UniversityLhasa 850000China Institute for Nuclear Research of the Russian Academy of sciences Moscow 117312Russia school of Physical science and Technology Southwest Jiaotong UniversityChengdu 610031China school of Management science and Engineering Hebei University of Economics and BusinessShijiazhuang 050061China Moscow Institute of Physics and Technology Moscow 141700Russia

By accurately measuring composition and energy spectrum of cosmic ray,the origin problem of so called“knee”region(energy>one PeV)can be ***,up to the present,the results of the spectrum in the knee region obtained by several previous experiments have shown obvious differences,so they cannot give effective evidence for judging the theoretical models on the origin of the ***,the Large High Altitude Air shower Observatory(LHAAsO)has reported several major breakthroughs and important results in astro-particle physics *** on its advantages of wide-sky survey,high altitude location and large area detector arrays,the research content of LHAAsO experiment mainly includes ultra high-energy gamma-ray astronomy,measurement of cosmic ray spectra in the knee region,searching for dark matter and new phenomena of particle physics at higher *** electron and thermal neutron detector(EN-Detector)is a new scintillator detector which applies thermal neutron detection technology to measure cosmic ray extensive air shower(EAs).This technology is an extension of *** EN-Detector Array(ENDA)can highly efficiently measure thermal neutrons generated by secondary hadrons so called“skeleton”of *** this paper,we perform the optimization of ENDA configuration,and obtain expectations on the ENDA results,including thermal neutron distribution,trigger efficiency and capability of cosmic ray composition *** obtained real data results are consistent with those by the Monte Carlo simulation.

关键词： cosmic ray EAs knee region LHAAsO ENDA

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Recent advances in laser self-injection locking to high-Q microresonators

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Frontiers of physics 2023年第2期18卷 89-130页

作者： Nikita M.Kondratiev Valery E.Lobanov Artem E.shitikov Ramzil R.Galiev Dmitry A.Chermoshentsev Nikita Yu.Dmitriev Andrey N.Danilin Evgeny A.Lonshakov kirill N.Min’kov Daria M.sokol steevy J.Cordette Yi-Han Luo Wei Liang Junqiu Liu Igor A.Bilenko Directed Energy Research Centre Technology Innovation InstituteAbu DhabiUnited Arab Emirates Russian Quantum Center MoscowRussia skolkovo Institute of science and Technology SkolkovoRussia Moscow Institute of Physics and Technology DolgoprudnyMoscow RegionRussia Faculty of Physics Lomonosov Moscow State UniversityMoscowRussia shenzhen Institute for Quantum science and Engineering Southern University of Science and TechnologyShenzhen 518055China International Quantum Academy Shenzhen 518048China Key Laboratory of Nanodevices and Applications Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China Hefei National Laboratory University of Science and Technology of ChinaHefei 230026China

The stabilization and manipulation of laser frequency by means of an external cavity are nearly ubiquitously used in fundamental research and laser applications. While most of the laser light transmits through the cavity, in the presence of some back-scattered light from the cavity to the laser, the self-injection locking effect can take place, which locks the laser emission frequency to the cavity mode of similar frequency. The self-injection locking leads to dramatic reduction of laser linewidth and noise. Using this approach, a common semiconductor laser locked to an ultrahigh-Q microresonator can obtain sub-Hertz linewidth, on par with state-of-the-art fiber lasers. Therefore it paves the way to manufacture high-performance semiconductor lasers with reduced footprint and cost. Moreover, with high laser power, the optical nonlinearity of the microresonator drastically changes the laser dynamics, offering routes for simultaneous pulse and frequency comb generation in the same microresonator. Particularly, integrated photonics technology, enabling components fabricated via semiconductor CMOs process, has brought increasing and extending interest to laser manufacturing using this method. In this article, we present a comprehensive tutorial on analytical and numerical methods of laser self-injection locking, as well a review of most recent theoretical and experimental achievements.

关键词： self-injection locking laser stabilization microresonator nonlinearity single-frequency lasing multi-frequency lasing

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Dielectric Mie voids: confining light in air

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Light(science & Applications) 2023年第2期12卷 156-167页

作者： Mario Hentsche Kirll Koshelev Florian sterl steffen Botho Julian Karst Lida shamsafar Thomas Weiss Yuri Kivshar Harald Giessen 4th Physics Institute and Research Center sCoPE University of StuttgartPfaffenwaldring 5770569 StuttgartGermany Nonlinear Physics Centre Research School of PhysicsAustralian National UniversityCanberraACT 2601Australia institute of Physics University of Grazand NAWI GrazUniversitatsplatz 58010 GrazAustria

Manipulating light on the nanoscale has become a central challenge in metadevices,resonant surfaces,nanoscale optical sensors,and many more,and it is largely based on resonant light confinement in dispersive and lossy metals and ***,we experimentally implement a novel strategy for dielectric nanophotonics:Resonant subwavelength localized confinement of light in *** demonstrate that voids created in high-index dielectric host materials support localized resonant modes with exceptional optical *** to the confinement in air,the modes do not suffer from the loss and dispersion of the dielectric host *** experimentally realize these resonant Mie voids by focused ion beam milling into bulk silicon wafers and experimentally demonstrate resonant light confinement down to the UV spectral range at 265 nm(4.68 eV).Furthermore,we utilize the bright,intense,and naturalistic colours for nanoscale colour *** voids will thus push the operation of functional high-index metasurfaces into the blue and UV spectral *** combination of resonant dielectric Mie voids with dielectric nanoparticles will more than double the parameter space for the future design of metasurfaces and other micro-and nanoscale optical *** particular,this extension will enable novel antenna and structure designs which benefit from the full access to the modal field inside the void as well as the nearly free choice of the high-index material for novel sensing and active manipulation strategies.

关键词： dielectric utilize exceptional

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The Role of Multilateral Development Banks and Institutions Are Important

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China's Foreign Trade 2016年第5期 13-页

作者： kirill Dmitriev b20 infrastructure taskforce russian direct investment fund

These five recommendations cover the most critical aspects of infrastructure development:an attractive pipeline of projects,diversity of financial instruments and a conducive regulatory environment,the role of multila... 详细信息

关键词： The Role of Multilateral Development Banks and Institutions Are Important

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CAsCIRE surveillance network and work on avian influenza viruses

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science China(Life sciences) 2017年第12期60卷 1386-1391页

作者： Yuhai Bi Weifeng shi Jianjun Chen Quanjiao Chen Zhenghai Ma Gary Wong Wenxia Tian Renfu Yin Guanghua Fu Yongchun Yang William J.Liu Chuansong Quan Qianli Wang shenghu He Xiangdong Li Qianfeng Xia Lixin Wang Zhaohui Pan Laixing Li Hong Li Wen Xu Ying Luo Hui Zeng Lianpan Dai Haixia Xiao kirill sharshov Alexander shestopalov Yi shi Jinghua Yan Xuebing Li Yingxia Liu Fumin Lei Wenjun Liu George F.Gao CAs Key Laboratory of Pathogenic Microbiology and Immunology Collaborative Innovation Center for Diagnosis and Treatment ofInfectious DiseaseInstitute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)Chinese Academy of SciencesBeijing 100101China shenzhen Key Laboratory of Pathogen and Immunity State Key Discipline of Infectious DiseaseShenzhen Third People's HospitalShenzhen 518112China shandong Universities Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases Taishan Medical CollegeTai'an 271016China CAs Key Laboratory of special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China College of Life science and Technology Xinjiang UniversityUrumchi 830046China College of Animal science and Veterinary Medicine Shanxi Agricultural UniversityTaigu 030801China Department of Veterinary Preventive Medicine College of Veterinary MedicineJilin UniversityJilin 130062China Institute of Animal Husbandry and Veterinary Medicine Fujian Academy of Agricultural SciencesFuzhou 350013China Center of Excellence for Animal Health Inspection College of Animal Science and TechnologyZhejiang Agriculture and Forestry UniversityZhejiang 311300China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Key Laboratory of Public Health safety Ministry of EducationSchool of Public HealthFudan UniversityShanghai 200032China Laboratory of Clinical Veterinary Medicine College of AgricultureNingxia UniversityYinchuan 750021China National Research Center for Veterinary Medicine Luoyang 471003China Laboratory of Tropical Biomedicine and Biotechnology and Faculty of Tropical Medicine and Laboratory MedicineImmunology DepartmentSchool of Basic Medicine and Life ScienceHainan Medical UniversityHaikou 571101China Xizang Agriculture and Animal Husbandry College Linzhi 860000China Key Laboratory of Adaptation and Evolution of Plateau Biota N

studies on influenza virus by Chinese Academy of sciences(CAs)could be traced back as early as 2005 by the CAs Key Laboratory of Pathogenic Microbiology and Immunology(CAsPMI),who discovered that Qinghai-like Clade 2.2H5N1 subtype highly pathogenic avian influenza virus(HPAIV)first caused severe outbreak in wild birds in Qinghai Lake(Liu et al.,2005).

关键词： CAsCIRE surveillance network work on avian influenza viruses

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俄罗斯转向东方:历史意义与现实任务

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俄罗斯学刊 2024年第4期14卷 70-97页

作者： [俄]基里尔·巴巴耶夫胡巍葳(编译) 俄罗斯科学院中国与现代亚洲研究所黑龙江大学俄罗斯语言文学与文化研究中心

作为自称为“国家-文明”的伟大欧亚大国,俄罗斯正在为欧亚大陆和整个世界赋予新的内涵,并迅速调集其资源以切实实施新东方战略。现代俄罗斯外交政策的这一主要趋势在短时间内不仅会令俄罗斯发生显著变化,而且会改变整个地缘政治状况,... 详细信息

作为自称为“国家-文明”的伟大欧亚大国,俄罗斯正在为欧亚大陆和整个世界赋予新的内涵,并迅速调集其资源以切实实施新东方战略。现代俄罗斯外交政策的这一主要趋势在短时间内不仅会令俄罗斯发生显著变化,而且会改变整个地缘政治状况,形成新的国际关系体系。真正的“转向东方”是在科学和技术、教育和文化上的根本转向。至于各国人民之间的互动水平、人文联系以及总体上的文化和文明互动领域的转变远未完成,其任务仍然极为紧迫。俄罗斯完成“转向东方”政策的主要任务,可以在2030年之前完全平衡俄罗斯的外交政策和对外经济活动,消除来自西方的大部分制裁带来的风险,显著提高俄罗斯在东方国家进行各类活动的效果。

关键词：俄中关系外交政策对外经济活动俄罗斯

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Genome and clonal hematopoiesis stability contrasts with immune,cfDNA,mitochondrial,and telomere length changes during short duration spaceflight

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Precision Clinical Medicine 2024年第1期7卷 1-13页

作者： J.sebastian Garcia-Medina Karolina sienkiewicz s.Anand Narayanan Eliah G.Overbey kirill Grigorev Krista A.Ryon Marissa Burke Jacqueline Proszynski Braden Tierney Caleb M.schmidt Nuria Mencia-Trinchant Remi Klotz Veronica Ortiz Jonathan Foox Christopher Chin Deena Najjar Irina Matei Irenaeus Chan Carlos Cruchaga Ashley Kleinman JangKeun Kim Alexander Lucaci Conor Loy Omary Mzava Iwijn De Vlaminck Anvita singaraju Lynn E.Taylor Julian C.schmidt Michael A.schmidt Kelly Blease Juan Moreno Andrew Boddicker Junhua Zhao Bryan Lajoie Andrew Altomare semyon Kruglyak shawn Levy Min Yu Duane C.Hassane susan M.Bailey Kelly Bolton Jaime Mateus Christopher E.Mason Department of Physiology and Biophysics Weill Cornell MedicineCornell UniversityNew YorkNY 10021USA The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine Weill Cornell MedicineNew YorkNY 10021USA Department of Immunology Weill Cornell MedicineCornell UniversityNew YorkNY 10021USA Department of stem Cell Biology and Regenerative Medicine Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90033USA space Exploration Technologies Corporation HawthomeCA 90250USA sovaris Aerospace BoulderCO 80302USA Advanced Pattern Analysis&Human Performance Group BoulderCO 80302USA Children's Cancer and Blood Foundation Laboratories Departments of Pediatrics and Cell and Developmental BiologyDrukier Institute for Children's HealthMeyer Cancer CenterWeill Cornell MedicineNew YorkNY 10021USA Meyer Cancer Center Weill Cornell MedicineNew YorkNY 10065USA Department of Nutrition and Integrative Physiology Florida State UniversityTallahasseeFL 32306USA Department of systems Engineering Colorado State UniversityFort CollinsCO 80523USA Element Biosciences San DiegoCA 10055USA Department of Environmental and Radiological Health sciences Colorado State UniversityFort CollinsCO 80523USA Cell and Molecular Biology Program Colorado State UniversityFort CollinsCO 80523USA BioAstra Inc New YorkNYUSA Washington University st Louis Oncology Division St.LouisMO 63100USA Meinig school of Biomedical Engineering Cornell UniversityIthacaNY 14853USA The Feil Family Brain and Mind Research Institute Weill Cornell MedicineNY 10021USA WorldQuant Initiative for Quantitative Prediction Weill Cornell MedicineNew YorkNY 10021USA

Background The Inspiration4(I4)mission,the first all-civilian orbital flight mission,investigated the physiological effects of short-duration spaceflight through a multi-omic *** advances,there remains much to learn about human adaptation to spaceflight's unique challenges,including microgravity,immune system perturbations,and radiation *** To provide a detailed genetics analysis of the mission,we collected dried blood spots pre-,during,and post-flight for DNA *** length was measured by quantitative PCR,while whole genome and cfDNA sequencing provided insight into genomic stability and immune adaptations.A robust bioinformatic pipeline was used for data analysis,including variant calling to assess mutational *** Telomere elongation occurred during spaceflight and shortened after return to ***-free DNA analysis revealed increased immune cell signatures *** significant clonal hematopoiesis of indeterminate potential(CHIP)or whole-genome instability was *** long-term gene expression changes across immune cells suggested cellular adaptations to the space environment persisting months *** Our findings provide valuable insights into the physiological consequences of short-duration spaceflight,with telomere dynamics and immune cell gene expression adapting to spaceflight and persisting after return to *** sequencing data will serve as a reference point for studying the early development of CHIP in astronauts,an understudied phenomenon as previous studies have focused on career *** study will serve as a reference point for future commercial and non-commercial spaceflight,low Earth orbit(LEO)missions,and deep-space exploration.

关键词： genomes clonal hematopoiesis stability immune mitochondria ribosomes spaceflight

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PandaX: a liquid xenon dark matter experiment at CJPL

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science China(Physics,Mechanics & Astronomy) 2014年第8期57卷 1476-1494页

作者： CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI shaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUsHKIN kirill REN XiangXiang sCHUBNELL Michael sHEN ManBing sHI YuJie sTEPHENsON scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU shiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua shanghai Institute of Applied Physics The Institute of Nuclear and Particle Physics Astronomy and Cosmology (INPAC) and Department of Physics and AstronomyShanghai Jiao Tong University Yalong River Hydropower Development Company Ltd. school of Physics and Key Laboratory of Particle Physics and Particle Irradiation (MOE) Shandong University school of Physics Peking University Department of Physics University of Maryland College Park MD 20742 USA school of Mechanical Engineering Shanghai Jiao Tong University Department of Physics University of Michigan Ann Arbor MI 48109 USA

PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay *** central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton *** experiment is located in the Jinping Deep-Underground Laboratory in sichuan,*** detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional *** this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented.

关键词： dark matter liquid xenon detector underground experiment time projection chamber

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