检索结果-南通市图书馆

Soil and water conservation measures improve soil carbon sequestration and soil quality under cashews

International Journal of Sediment Research 2021年第2期36卷 190-206页

作者： Gopal Ramdas Mahajan Bappa Das Sandrasekaran Manivannan Begur Lakshminarasimha Manjunath Ram Ratan Verma Sujeet Desai Rahul Mukund Kulkarni Ashish Marotrao Latar Reshma Sale Dayesh Murgaonkar kiran puna patel Shaiesh Morajkar Ashwini Desai Natasha Barnes Heena Mulla Natural Resource Management ICAR-Central Coastal Agricultural Research Institute ICAR-Indian Institute of Soil and Water Conservation Regional Centre Division of Fruit Crops ICAR-Indian Institute of Horticultural Research Division of Crop Production Indian Institute of Sugarcane Research Institute of Agricultural Sciences Banaras Hindu University Biological Oceanography Division CSIReNational Institute of Oceanography Goa University

Land degradation is becoming a serious problem in the west coast region of India where one of the world’s eight biodiversity hotspots, the ‘Western Ghats’, is present. Poor land management practices and high rainfall have led to increasing problems associated with land degradation. A long-term(13-year)experiment was done to evaluate the impact of soil and water conservation measures on soil carbon sequestration and soil quality at three different depths under cashew nut cultivation on a 19% slope. Five soil and water conservation measures-continuous contour trenches, staggered contour trenches, halfmoon terraces, semi-elliptical trenches, and graded trenches all with vegetative barriers of Stylosanthes scabra and Vetiveria zizanoides and control were evaluated for their influence on soil properties,carbon sequestration, and soil quality under cashews. The soil and water conservation measures improved significantly the soil organic carbon, soil organic carbon stock, carbon sequestration rate and microbial activity compared to the control condition(without any measures). Among the measures tested, continuous contour trenches with vegetative barriers outperformed the others with respect to soil organic carbon stock, sequestration rate, and microbial activity. The lower metabolic quotient with the measures compared to the control indicated alleviation of environmental stress on microbes. Using principal component analysis and a correlation matrix, a minimum dataset was identified as the soil available nitrogen, bulk density, basal soil respiration, soil p H, acid phosphatase activity, and soil available boron and these were the most important soil properties controlling the soil quality. Four soil quality indices using two summation methods(additive and weighted) and two scoring methods(linear and non-linear) were developed using the minimum dataset. A linear weighted soil quality index was able to statistically differentiate the effect of soil and water conservation me

关键词： Land degradation Microbial activity Microbial biomass carbon Minimum dataset Soil erosion Western Ghats

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

细粒棘球绦虫乳酸脱氢酶基因的生物信息学分析及原核表达

引用

中国病原生物学杂志 2024年第5期19卷 555-561页

作者：赵文卿薄新文普娜张玉霞陈旭珂张艳艳孙艳齐萌王正荣塔里木大学动物科学与技术学院新疆阿拉尔843300 省部共建绵羊遗传改良与健康养殖国家重点实验室/新疆农垦科学院畜牧兽医研究所石河子大学动物科技学院

目的了解细粒棘球绦虫的乳酸脱氢酶蛋白基因的生物信息学特性,并进行原核表达。方法利用NCBI网站登录的细粒棘球绦虫乳酸脱氢酶基因序列设计特异性引物和qRT-PCR引物,使用PCR扩增细粒棘球绦虫乳酸脱氢酶基因,使用荧光定量法检测细粒棘... 详细信息

目的了解细粒棘球绦虫的乳酸脱氢酶蛋白基因的生物信息学特性,并进行原核表达。方法利用NCBI网站登录的细粒棘球绦虫乳酸脱氢酶基因序列设计特异性引物和qRT-PCR引物,使用PCR扩增细粒棘球绦虫乳酸脱氢酶基因,使用荧光定量法检测细粒棘球绦虫乳酸脱氢酶在不同发育阶段的相对表达量,使用生物信息学软件对细粒棘球绦虫乳酸脱氢酶蛋白进行分析;构建重组pET28a-EgLdh质粒,转进大肠埃希菌BL21(DE3)感受态细胞,利用SDS-PAGE检测蛋白的表达,并通过Western blot对蛋白进行免疫原性分析;利用原核表达重组pET28a-EgLDH蛋白免疫小鼠制备多克隆抗体,通过间接免疫荧光分析其在虫体上的分布。结果生物信息学分析发现EgLDH全长996 bp,编码331个氨基酸,分子式为C_(1575)H_(2561)N_(425)O_(470)S_(17),相对分子质量为35516.25,理论等电点为6.32,不稳定指数为27.96,显示为稳定蛋白,脂肪系数为106.83,亲水性为0.236,为疏水性蛋白,有13个潜在的B细胞抗原表位;通过双酶切鉴定及测序分析证明,pET28a-EgLDH重组质粒构建成功。重组质粒pET28a-EgLDH转化大肠埃希菌BL21(DE3)感受态细胞后使用IPTG成功诱导表达蛋白,分子质量约为40 ku,Western blot结果显示,重组蛋白能被感染棘球蚴的犬阳性血清识别,表明其均具有良好的反应原性。EgLDH在细粒棘球绦虫阶段的相对转录水平显著高于原头蚴阶段。间接免疫荧光分析显示其定位在原头蚴的表皮层。结论rEgLDH具有较好的免疫活性,是一种潜在的疫苗候选抗原。

关键词：细粒棘球绦虫 EgLDH基因生物信息学分析原核表达

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Amphibians and Reptiles of Cebu,Philippines：The Poorly Understood Herpetofauna of an Island with Very Little Remaining Natural Habitat

引用

Asian Herpetological Research 2016年第3期7卷 151-179页

作者： Christian E.SUPSUP Nevong M.puna Augusto A.ASIS Bernard R.REDOBLADO Maria Fatima G.PANAGUINIT Faith M.GUINTO Edmund B.RICO Arvin C.DIESMOS Rafe M.BROWN Neil Aldrin D.MALLARI Fauna and Flora International- Philippines Foggy Heights Subdivision Brgy. San Jose Tagaytay City Philippines Biology Department De La Salle University 2401 Taft Avenue Manila Philippines Cebu Biodiversity Conservation Foundation 41 Edison Street Lahug Cebu City Cebu Cebu Technological University - Argao Campus Ed Kintanar Street Lamacan 6021 Argao Cebu Philippine National Museum Zoology Division Herpetology Section. Rizal Park Buvg os St. Manila Philippines Department of Ecology and Evolutionary Biology and KU Biodiversity Institute University of Kansas Lawrence Kansas 66045 USA

Despite its proximity to other well studied islands, Cebu has received little attention from herpetologists, most likely because of early deforestation and the perception very little natural habitat remains for amphibians and reptiles. In this study, we present a preliminary assessment of island＇s herpetofauna, focusing our field work on Cebu＇s last remaining forest fragments and synthesizing all available historical museum distribution data. We surveyed amphibians and reptile populations using standardized methods to allow for comparisons between sites and assess sufficiency of sampling effort. Fieldwork resulted in a total of 27 species recorded from five study sites, complementing the 58 species previously known from the island. Together, our data and historical museum records increase the known number of Cebu＇s resident species to 13 amphibians（frogs） and 63 reptiles（lizards, snakes, turtle, crocodile）. We recorded the continued persistence Cebu＇s rare and endemic lizard（Brachymeles cebuensis） and secretive snakes such as Malayotyphlops hypogius, and Ramphotyhlops cumingii, which persist despite Cebu＇s long history of widespread and continuous habitat degradation. Most species encountered, including common and widespread taxa, appeared to persist at low population abundances. To facilitate the immediate recovery of the remaining forest fragments, and resident herpetofauna, conservation effort must be sustained. However, prior to any conservation interventions, ecological baselines must be established to inform the process of recovery.

关键词： persist likely historical habitat remaining resident island facilitate museum populations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

湖北麻城示范县0～5岁小儿腹泻病原学监测

引用

同济医科大学学报 1991年第6期20卷 413-415页

作者：杨璞娜刘筱娴胡盛平陈树坤邱功合邢桂芝同济医科大学公共卫生学院卫生统计学教研室湖北麻城卫生防疫站

1986年7月至1987年6月,对湖北省麻城示范县308例0～5岁急性腹泻婴幼儿和185名同龄健康婴幼儿粪便作了病原体检测。结果发现,腹泻儿粪便病原体总检出率为61.7％,健康儿为18.4％。前者粪便中轮状病毒、志贺菌和致泻性大肠杆菌检出率分别为... 详细信息

1986年7月至1987年6月,对湖北省麻城示范县308例0～5岁急性腹泻婴幼儿和185名同龄健康婴幼儿粪便作了病原体检测。结果发现,腹泻儿粪便病原体总检出率为61.7％,健康儿为18.4％。前者粪便中轮状病毒、志贺菌和致泻性大肠杆菌检出率分别为18.5％、15.9％和12.3％,后者的致泻性大肠杆菌和轮状病毒检出率分别为6.5％和4.9％。冬春季轮状病毒阳性率最高,而夏秋季志贺菌阳性率最高。

关键词：腹泻病原学监测婴幼儿

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

湖北钟祥地区当地居民与河南移民食物中霉菌污染的对比分析

引用

武汉医学院学报 1982年第1期 35-39页

作者：杨璞娜贾桂珍梁浩材黄渭铭武汉医学院卫生系肿瘤流行病学和病因学研究室

1966～1969年河南淅川县五万多人移居到湖北省钟祥县大柴湖公社,分居四个管理区,该公社原有两个管理区为本地老户,有两万多人。移民原籍淅川县为食管癌高发区,移民地钟祥县为食管癌低发区。1974年普查中移民的食管癌患病率为本地老户10... 详细信息

1966～1969年河南淅川县五万多人移居到湖北省钟祥县大柴湖公社,分居四个管理区,该公社原有两个管理区为本地老户,有两万多人。移民原籍淅川县为食管癌高发区,移民地钟祥县为食管癌低发区。1974年普查中移民的食管癌患病率为本地老户10倍,移居8年后,移民食管癌标化死亡率

关键词：食管癌面粉酒曲检出率食管肿瘤糖化剂霉菌污染食物河南

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：