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Comparative In Vitro Osteoinductivity Study of CaP Ceramics (HA, α-TCP, β-TCP) using 10T1/2 Cells with Different Controls and Possible Correlations with Other Systems

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Journal of Biomaterials and Nanobiotechnology 2011年第2期2卷 162-171页

作者： Juliana Tsz Yan Lee Kefeng Wang Wai Hung TSANG king lau chow 不详

In this study, we use a pluripotent mesenchymal stem cell (MSC) model, C3H/10T1/2, to evaluate three calcium phos-phate (CaP) materials, namely the hydroxyapatite (HA), α-tricalcium phosphate (α-TCP) and β-tricalcium phosphate (β-TCP). 10T1/2 cell was chosen as it has advantages over its counterparts in terms of ease of maintenance, free of ethical concerns and also more reproducible results. ALP enzymatic assay, RT-qPCR, DAPI staining and SEM were employed to assess the osteoinductivity of these materials. A good reference material which also acts as a scientific control is necessary for comparisons of results from different experimental batches and hence other materials such as titanium, Nunclon plastic surface, BD Falcon plastic surface and gold coated porous HA were also tested. The results show that ceramics induce a more sustained osteo-differentiation state as compared with plastics. Inductivity was found to be acting in descending order of strength with HA > β-TCP > α-TCP, which is reversed in terms of their impact on proliferation rate (HA TCP) and in vivo osteoinductivity in terms of incidence and quality of bone described previously (HA > β-TCP > α-TCP). These confirm the suitability of using 10T1/2 cells in cell culture assay of osteoinductivity.

关键词： Bone Tissue Engineering Stem Cell Calcium Phosphate Hydroxyapatite Gene Expression Osteo-Inductivity In Vitro Test Pcr (Polymerase Chain Reaction) Sem (Scanning Electron Microscopy)

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Cellular & Molecular Immunology 2021年第4期18卷 1005-1015页

作者： Xuezhen Zeng Jingying Zhou Zhewen Xiong Hanyong Sun Weiqin Yang Myth T.S.Mok Jing Wang Jingqing Li Man Liu Wenshu Tang Yu Feng Hector Kwong-Sang W ang Shun-Wa Tsang king-lau chow Philip Chun Yeung John Wong Paul Bo-San Lai Anthony Wing-Hung Chan Ka Fai To Stephen Lam Chan Qiang Xia Jing Xue Xiao Chen Jun Yu Sui Peng Joseph Jao-Yiu Sung Ming Kuang Alfred Sze-Lok Cheng School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina lnstitute of Precision Medicine The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Liver Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Pharmacy The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Anatomical and Cellular Pathology The Chinese University of Hong KongHong Kong SARChina Department of Liver Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Gastroenterology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Division of Life Science Hong Kong University of Science and TechnologyHong Kong SARChina Department of Surgery The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Translational Oncology The Chinese University of Hong KongHong Kong SARChina Department of Clinical Oncology The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Oncogenes and Related Genes Stem Cell Research CenterRen Ji HospitalSchool of MedicineShanghai Cancer InstituteShanghai Jiao Tong UniversityShanghaiChina Department of Medicine and Therapeutics The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Digestive Disease The Chinese University of Hong KongHong Kong SARChina Clinical Trial Unit The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Cancer Center The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina

The liver is an immunologically tolerant organ and a common metastatic site of multiple cancer *** a role for cancer cell invasion programs has been well characterized,whether and how liver-intrinsic factors drive metastatic spread is incompletely ***,we show that aberrantly activated hepatocyte-intrinsic cell cycle-related kinase(CCRK)signaling in chronic liver diseases is critical for cancer metastasis by reprogramming an immunosuppressive *** an inducible liverspecific transgenic model,we found that CCRK overexpression dramatically increased both B16F10 melanoma and MC38 colorectal cancer(CRC)metastasis to the liver,which was highly infiltrated by polymorphonuclear-myeloid-derived suppressor cells(PMNMDSCs)and lacking natural killer T(NKT)*** of PMN-MDSCs in CCRK transgenic mice restored NKT cell levels and their interferon gamma production and reduced liver metastasis to 2.7% and 0.7%(metastatic tumor weights)in the melanoma and CRC models,***,CCRK activated nuclear factor-kappa B(NF-κB)signaling to increase the PMN-MDSC trafficking chemokine C-X-C motif ligand 1(CXCL1),which was positively correlated with liver-infiltrating PMN-MDSC levels in CCRK transgenic ***,CRC liver metastasis patients exhibited hyperaaivation of hepatic CCRK/NF-κB/CXCL1 signaling,which was associated with accumulation of PMN-MDSCs and paucity of NKT cells compared to healthy liver transplantation *** summary,this study demonstrates that immunosuppressive reprogramming by hepatic CCRK signaling undermines antimetastatic *** findings offer new mechanistic insights and therapeutic targets for liver metastasis intervention.

关键词： Cell cycle related kinase liver metastasis liver immune microenvironment myeloid-derived suppressor cell natural killer T cell

Regulation of cytohesins by their interactors in the nervous system

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Neural Regeneration Research 2023年第10期18卷 2186-2187页

作者： Yuqi Zhai king To Leung Kwok-Fai lau School of Life Sciences Faculty of ScienceThe Chinese University of Hong KongHong Kong Special Administrative RegionChina

Adenosine diphosphate ribosylation factors(ARFs) are small GTP-binding proteins of the Ras superfamily which are involved in membrane trafficking and actin cytoskeleton *** other small GTPases,their functions are attributed to the cycling of ARF between GTP(active) and GDP (inactive) states through the actions of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins,respectively.

关键词： Adenosine cycling attributed

Cosmic microwave background constraints on the tensor-to-scalar ratio

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Research in Astronomy and Astrophysics 2014年第6期14卷 635-647页

作者： king lau Jia-Yu Tang Ming-Chung Chu Department of Physics and Institute of Theoretical Physics The Chinese University of Hong Kong

One of the main goals of modern cosmic microwave background （CMB） missions is to measure the tensor-to-scalar ratio r accurately to constrain inflation models. Due to ignorance about the reionization history Xe （z）, this analysis is usu- ally done by assuming an instantaneous reionization Xe （z） which, however, can bias the best-fit value of r. Moreover, due to the strong mixing of B-mode and E-mode polarizations in cut-sky measurements, multiplying the sky coverage fraction fsky by the full-sky likelihood would not give satisfactory results. In this work, we forecast constraints on r for the Planck mission taking into account the general reionization scenario and cut-sky effects. Our results show that by applying an N-point interpo- lation analysis to the reionization history, the bias induced by the assumption of in- stantaneous reionization is removed and the value of r is constrained within 5% error level, if the true value of r is greater than about 0.1.

关键词： cosmology: cosmic microwave background -- cosmology: cosmologicalparameters -- cosmology: early universe -- gravitational waves

Giant two-photon absorption of anatase TiO_(2) in Au/TiO_(2) core-shell nanoparticles

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光子学研究:英文版 2023年第7期11卷 1303-1313页

作者： LIJIE WANG TSZ HIM chow MALTE OPPERMANN JIANFANG WANG MAJED CHERGUI Laboratory of Ultrafast Spectroscopy ISIC and Lausanne Centre for Ultrafast Science(LACUS)École Polytechnique Fédérale de Lausanne(EPFL)CH-1015 LausanneSwitzerland Department of Physics The Chinese University of Hong KongShatinHong Kong SARChina Division of Physical Science and Engineering King Abdullah University of Science and Technology(KAUST)ThuwalKingdom of Saudi Arabia Chemistry Department University of Basel4001 BaselSwitzerland

We report on deep-to-near-UV transient absorption spectra of core-shell Au∕SiO_(2) and Au∕TiO_(2) nanoparticles(NPs) excited at the surface plasmon resonance of the Au core, and of UV-excited bare anatase TiO_(2) NPs. The bleaching of the first excitonic transition of anatase TiO_(2) at ~3.8 e V is a signature of the presence of electrons/holes in the conduction band(CB)/valence band(VB) of the material. We find that while in bare anatase TiO_(2) NPs, two-photon excitation does not occur up to the highest used fluences(1.34 mJ∕cm^(2)), it takes place in the TiO_(2) shell at moderate fluences(0.18 mJ∕cm^(2)) in Au∕TiO_(2) core-shell NPs, as a result of an enhancement due to the plasmon resonance. We estimate the enhancement factor to be of the order of ~10~8–10~9. Remarkably, we observe that the bleach of the 3.8 eV band of TiO_(2) lives significantly longer than in bare TiO_(2), suggesting that the excess electrons/holes in the conduction/valence band are stored longer in this material.

关键词： material absorption excited

Linewidth narrowing in self-injection-locked on-chip lasers

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Light(Science & Applications) 2023年第10期12卷 2074-2083页

作者： Emad Alkhazraji Weng W.chow Frédéric Grillot John E.Bowers Yating Wan Integrated Photonics Lab King Abdullah University of Science and TechnologyThuwal 23955Saudi Arabia Sandia National Laboratories AlbuquerqueNM 87185-1086USA LTCI Télécom ParisInstitut Polytechnique de Paris91120 PalaiseauFrance Department of Electronic and Computer Engineering University of California-Santa BarbaraSanta BarbaraCA 93106USA

Stable laser emission with narrow linewidth is of critical importance in many applications,including coherent communications,LIDAR,and remote *** this work,the physics underlying spectral narrowing of self-injection-locked on-chip lasers to Hz-level lasing linewidth is investigated using a composite-cavity *** integrated III–V/SiN lasers operating with quantum-dot and quantum-well active regions are analyzed with a focus on the effects of carrier quantum *** intrinsic differences are associated with gain saturation and carrier-induced refractive index,which are directly connected with 0-and 2-dimensional carrier densities of *** from parametric studies are presented for tradeoffs involved with tailoring the linewidth,output power,and injection current for different device *** both quantum-well and quantum-dot devices show similar linewidth-narrowing capabilities,the former emits at a higher optical power in the self-injection-locked state,while the latter is more ***,a multi-objective optimization analysis is provided to optimize the operation and design *** the quantum-well laser,minimizing the number of quantum-well layers is found to decrease the threshold current without significantly reducing the output *** the quantum-dot laser,increasing the quantum-dot layers or density in each layer increases the output power without significantly increasing the threshold *** findings serve to guide more detailed parametric studies to produce timely results for engineering design.

关键词： narrowing injection quantum

Visible light-induced degradation of organic pollutants using Fe(II) supported on silica gel as an effective catalyst

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Chinese Science Bulletin 2008年第10期53卷 1497-1502页

作者： ZHAO Chao HUANG YingPing FANG YanFen JIANG LiRong LIU LiMing king Tong lau Center for Ecologic and Environment Science China Three Gorges University Yichang 443002 China College of Chemistry Central China Normal University Wuhan 430070 China School of Chemical -Sciences National Centre for Sensor Research Dublin City University Dublin Ireland

Silica gel-supported Fe(II) (SiOFe) was prepared and used for heterogeneous degradation of sulforho-damine B (SRB) and 2,4-dichlorophenol (DCP) under visible irradiation (λ > 420 nm) as an effective catalyst. UVvisible spectra, and infrared Spectrophotometry (IR), fluorescence, total organic carbon (TOC) and electron spin resonance (ESR) measurements were employed to analyze the photoreaction products. The results showed that SRB could be efficiently degraded by SiOFe/H2O2 system under visible irradiation with 100% decolorization and 72.3% TOC removal after 180 min illumination. The results of ESR and fluorescence measurements indicated that the oxidative process was predominated mainly by the hydroxyl radical (·OH) generated in the system.

关键词：二氧化硅凝胶剂异质性光催化剂辐射度

Fe65-engineered neuronal exosomes encapsulating corynoxine-B ameliorate cognition and pathology of Alzheimer’s disease

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Signal Transduction and Targeted Therapy 2023年第11期8卷 5481-5494页

作者： Ashok Iyaswamy Abhimanyu Thakur Xin-Jie Guan Senthilkumar Krishnamoorthi Tsz Yan Fung Kejia Lu Isha Gaurav Zhijun Yang Cheng-Fu Su Kwok-Fai lau Kui Zhang Roy Chun-Laam Ng Qizhou Lian king-Ho Cheung Keqiang Ye Huanhuan Joyce Chen Min Li Mr.&Mrs.Ko Chi-Ming Centre for Parkinson’s Disease Research School of Chinese MedicineHong Kong Baptist UniversityHong Kong SARChina Pritzker School of Molecular Engineering The University of ChicagoChicagoILUSA Ben May Department for Cancer Research The University of ChicagoChicagoILUSA School of Chinese Medicine Hong Kong Baptist UniversityHong Kong SARChina School of Life Sciences The Chinese University of Hong KongHong Kong SARChina Division of Neuroscience School of Biological SciencesFaculty of BiologyMedicine and HealthManchester Academic Health Science CentreUniversity of ManchesterManchesterUnited Kingdom Department of Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongPok Fu LamHong Kong SARChina Prenatal Diagnostic Center and Cord Blood Bank Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhouChina HKUMed Laboratory of Cellular Therapeutics the University of Hong KongPok Fu LamHong Kong SARChina Faculty of Life and Health Sciences Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina

Alzheimer’s disease(AD)is a neurodegenerative disorder characterized by the predominant impairment of neurons in the hippocampus and the formation of amyloid plaques,hyperphosphorylated tau protein,and neurofibrillary tangles in the *** overexpression of amyloid-βprecursor protein(APP)in an AD brain results in the binding of APP intracellular domain(AICD)to Fe65 protein via the C-terminal Fe65-PTB2 interaction,which then triggers the secretion of amyloid-βand the consequent pathogenesis of ***,targeting the interaction between APP and Fe65 can offer a promising therapeutic approach for ***,exosome,a type of extracellular vesicle with diameter around 30–200 nm,has gained much attention as a potential delivery tool for brain diseases,including AD,due to their ability to cross the blood–brain barrier,their efficient uptake by autologous cells,and their ability to be surface-modified with target-specific receptor ***,the engineering of hippocampus neuron cell-derived exosomes to overexpress Fe65,enabled the development of a novel exosome-based targeted drug delivery system,which carried Corynoxine-B(Cory-B,an autophagy inducer)to the APP overexpressed-neuron cells in the brain of AD *** Fe65-engineered HT22 hippocampus neuron cell-derived exosomes(Fe65-EXO)loaded with Cory-B(Fe65-EXO-Cory-B)hijacked the signaling and blocked the natural interaction between Fe65 and APP,enabling APP-targeted delivery of ***,Fe65-EXO-Cory-B induced autophagy in APP-expressing neuronal cells,leading to amelioration of the cognitive decline and pathogenesis in AD mice,demonstrating the potential of Fe65-EXO-Cory-B as an effective therapeutic intervention for AD.

关键词： Alzheimer gained loaded

Putting PLX5622 into perspective:microglia in central nervous system viral infection

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Neural Regeneration Research 2023年第6期18卷 1269-1270页

作者： Alanna G.Spiteri Nicholas J.C.king Viral Immunopathology Laboratory InfectionImmunity and Inflammation Research ThemeSchool of Medical SciencesFaculty of Medicine and HealthThe University of SydneySydneyNSWAustralia Sydney Cytometry The University of Sydney and Centenary InstituteSydneyNSWAustralia Ramaciotti Facility for Human Systems Biology The University of Sydney and Centenary InstituteSydneyNSWAustralia Charles Perkins Centre The University of SydneyAustralia The University of Sydney Institute for Infectious Diseases The University of SydneySydneyNSWAustralia The University of Sydney Nano Institute the University of SydneySydneyNSWAustralia

Elucidating the exact contribution of microglia to central nervous system(CNS)pathology has historically been extremely *** resident parenchymal myeloid cells are considered to have critical roles as frontline responders during pathogen invasion and CNS ***,understanding the precise temporal kinetics of microglial function is central to the evolution of novel therapeutics for disease intervention and/or resolution(Spiteri et al.,2022a).The development of PLX5622,a colony-stimulating factor 1 receptor(CSF-1R)inhibitor typically formulated into a rodent chow for simple oral administration has facilitated exploration of microglial functions in disease(Spangenberg et al.,2019).

关键词： invasion system precise