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栗疫病菌弱毒性菌株的筛选及其dsRNA的转化稳定性

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林业科学 2012年第9期48卷 95-98页

作者：朴春根李永 Lee Sanghyun kim kyunghee Lee Seungkyu 郭民伟中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京100091 韩国国立山林科学院森林病虫害科首尔130-712

为获取栗疫病生物防治的基础信息,本研究根据菌株在PDA培养基上培养7天后的菌丝生长速度、分生孢子形成能力等培养特性,从韩国国立山林科学院树木病理研究室保藏的60个栗疫病菌菌株中筛选了2个弱致病力菌株,进行dsRNA检测、弱致病力菌... 详细信息

为获取栗疫病生物防治的基础信息,本研究根据菌株在PDA培养基上培养7天后的菌丝生长速度、分生孢子形成能力等培养特性,从韩国国立山林科学院树木病理研究室保藏的60个栗疫病菌菌株中筛选了2个弱致病力菌株,进行dsRNA检测、弱致病力菌株和强致病力菌株间的细胞融合试验。结果表明:2个弱致病力菌株(KCP-135和KCP-136)中均检测到了dsRNA,弱毒性菌株KCP-22和其他19个强毒性菌株之间的菌落形成明显的隔离带并沿着隔离带产生分生孢子,没有明显的细胞融合现象,而弱毒性菌株KCP-22和强毒性菌株KCP-9之间的菌落则呈现了显著的细胞融合现象,而且其细胞融合菌株的培养特性和转化dsRNA数量均发生了变异。

关键词： Cryphonectria parasitica dsRNA 弱毒性栗疫病

栗疫病菌致病力和弱致病力菌株的分子生物学检测

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栗疫病菌致病力和弱致病力菌株的分子生物学检测

中国植物病理学会2006年学术年会

作者：朴春根 kim kyunghee Lee Sanghyun Lee Seungkyu 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室 Division of Forest Insect Pests and Diseases Korea Forest Research InstituteSeoul 130-712 Division of Forest Insect Pests and Diseases Korea Forest Research InstituteSeoul 130-712 Division of Forest Insect Pests and Diseases Korea Forest Research InstituteSeoul 130-712

根据栗疫病菌菌株的RAPD分子遗传标记分析结果,选用韩国的弱致病力菌株1个和美国的弱致病力菌株2个以及与此遗传背景相似的致病力菌株4个,通过rDNA-ITS区域的PCR-RFLP分析和RAPD分析,进行了分子生物学检测分析。利用通用引物ITSIF和ITS4... 详细信息

根据栗疫病菌菌株的RAPD分子遗传标记分析结果,选用韩国的弱致病力菌株1个和美国的弱致病力菌株2个以及与此遗传背景相似的致病力菌株4个,通过rDNA-ITS区域的PCR-RFLP分析和RAPD分析,进行了分子生物学检测分析。利用通用引物ITSIF和ITS4,以rDNA为模版扩增了720bp大小的ITS区域,并用AccII 等9个限制性内切酶进行了RFLP分析,但没能发现特异性RFLP片段。利用108个随机引物进行筛选的结果, 用随机引物OPC-07在致病力菌株中扩增了740bp的特异RAPD片段,但在弱致病力菌株中却未能扩增,即在本实验的范围内用这一特异性RAPD片段能区分栗疫病菌的致病力和弱致病力菌株。

关键词：栗疫病菌 Cryphonectria parasitica 弱致病力遗传标记

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栗疫病菌弱致病力的遗传变异分析

栗疫病菌弱致病力的遗传变异分析

Molecules “buckle up” to drive layered hybrid perovskites into single-crystal nanowires in water

中国植物病理学会2008年学术年会

作者：朴春根 Lee Seungkyu Lee Sanghyun kim kyunghee Byung-Ju Moon 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室

为了分析栗疫病菌的自然弱致病力菌株和通过细胞融合具有 dsRNA 的转化弱致病力菌株的遗传稳定性,对单孢后代进行了培养特性和 dsRNA 变异分析。结果表明,自然弱致病力菌株和转化弱致病力菌株的单孢分离后代都发生了菌丝生长、产孢能力... 详细信息

为了分析栗疫病菌的自然弱致病力菌株和通过细胞融合具有 dsRNA 的转化弱致病力菌株的遗传稳定性,对单孢后代进行了培养特性和 dsRNA 变异分析。结果表明,自然弱致病力菌株和转化弱致病力菌株的单孢分离后代都发生了菌丝生长、产孢能力、菌落颜色等培养特性的变化:自然弱致病力菌株和转化弱致病力菌株的 dsRNA 均能稳定地遗传给后代。

关键词：栗疫病菌 Cryphonectria parasitica 弱致病力变异

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Science China Chemistry 2025年第1期68卷 3-4页

作者： Seokhyoung kim Department of Chemistry Michigan State University

Layered 2D metal halide perovskites (MHPs) are one of the most exciting candidates for next-generation light sources owing to their outstanding optoelectronic properties. These properties are readily tunable by controlling the halide composition, number of inorganic layers, and the chemistry of organic cations. Another key element that can substantially impact the materials’ functionalities is crystal morphology [1].

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Role of glutathione transferase in phase separation of FUS and TAF15 in neurons

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Neural Regeneration Research 2025年第7期20卷 1999-2000页

作者： Kiyoung kim Department of Medical Science Soonchunhyang UniversityAsanKorea

Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(Cleveland and Rothstein,2001).Frontotemporal lobar degeneration(FTLD)is a neurodegenerative disease affecting the frontal and temporal lobes of the brain,leading to changes in behavior,personality,and language(Van Langenhove et al.,2012).Both ALS and FTLD are classified as proteinopathies in which abnormal protein aggregation and accumulation in neurons contribute to the disease *** in sarcoma(FUS)is a DNA/RNA-binding protein involved in various cellular processes,including transcriptional regulation,RNA splicing,and DNA *** in the FUS gene have been linked to familial ALS,highlighting the importance of FUS in the disease pathogenesis(Vance et al.,2009).In ALS and FTLD,aberrant post-translational modifications(PTMs)of FUS,such as phosphorylation,acetylation,and methylation,have been implicated in the promotion of FUS aggregation and neurotoxicity(Choi et al.,2023).Therefore,understanding the regulatory mechanisms of FUS PTMs is crucial for developing targeted therapies against these diseases.

关键词： degeneration FUS aggregation

Carboplatin restores neuronal toxicity in FUS-linked amyotrophic lateral sclerosis

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Neural Regeneration Research 2025年第8期20卷 2319-2320页

作者： Kiyoung kim Department of Medical Science Soonchunhyang UniversityAsanKorea

Amyotrophic lateral sclerosis(ALS),also known as Lou Geh rig's disease,is a progressive neurodegenerative disorder that affects motor neurons in the brain and spinal *** leads to muscle weakness,paralysis,and ultimate... 详细信息

关键词： paralysis sclerosis respiratory

Recovery of the injured neural system through gene delivery to surviving neurons in Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2855-2861页

作者： Chanchal Sharma Sehwan kim Hyemi Eo Sang Ryong kim School of Life Science Kyungpook National UniversityDaeguKorea BK21 FOUR KNU Creative BioResearch Group Kyungpook National University DaeguKorea Brain Science and Engineering Institute Kyungpook National UniversityDaeguKorea

A critical unaddressed problem in Parkinson’s disease is the lack of therapy that slows or hampers *** medications effectively manage symptoms,they offer no long-term benefit because they fail to address the underlying neuronal *** highlights that the elusive goals of halting progression and restoring damaged neurons limit the long-term impact of current *** clinical trials using gene therapy have demonstrated the safety of various vector delivery systems,dosages,and transgenes expressed in the central nervous system,signifying tangible and substantial progress in applying gene therapy as a promising Parkinson’s disease ***,at diagnosis,many dopamine neurons remain in the substantia nigra,offering a potential window for recovery and *** propose that modulating these surviving dopamine neurons and axons in the substantia nigra and striatum using gene therapy offers a potentially more impactful therapeutic approach for future ***,innovative gene therapies that focus on preserving the remaining elements may have significant potential for enhancing long-term outcomes and the quality of life for patients with Parkinson’s *** this review,we provide a perspective on how gene therapy can protect vulnerable elements in the substantia nigra and striatum,offering a novel approach to addressing Parkinson’s disease at its core.

关键词： adeno-associated virus gene therapy neuroprotection neurorestoration neurotrophic factor nigrostriatal dopamine pathway pro-survival protein

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产品造型和宗教文化

设计 2005年第5期21卷 168-168页

作者： kyunghee kim Dept Industrial design Semyung University

产品设计首先是一种特定时期的需求，拥有各种时代特征，与人的生活息息相关。本文将给产品设计赋予宗教文化的色彩，从科学的理论角度研究为什么我们必须将宗教注入产品造型以及我们该如何做。

关键词：宗教文化产品造型产品设计

High mobility group box 1 in the central nervous system:regeneration hidden beneath inflammation

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Neural Regeneration Research 2025年第1期20卷 107-115页

作者： Hanki kim Bum Jun kim Seungyon Koh Hyo Jin Cho Xuelian Jin Byung Gon kim Jun Young Choi Department of Brain Science Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Ajou University Graduate School of MedicineSuwonSouth Korea Department of Neurology Ajou University School of MedicineSuwonSouth Korea Department of Geriatrics The Affiliated Suqian First People’s Hospital of Nanjing Medical UniversitySuqianJiangsu ProvinceChina

High-mobility group box 1 was first discovered in the calf thymus as a DNA-binding nuclear protein and has been widely studied in diverse fields,including neurology and ***-mobility group box 1 in the extracellular space functions as a pro-inflammatory damage-associated molecular pattern,which has been proven to play an important role in a wide variety of central nervous system disorders such as ischemic stroke,Alzheimer’s disease,frontotemporal dementia,Parkinson’s disease,multiple sclerosis,epilepsy,and traumatic brain *** drugs that inhibit high-mobility group box 1 as a damage-associated molecular pattern,such as glycyrrhizin,ethyl pyruvate,and neutralizing anti-high-mobility group box 1 antibodies,are commonly used to target high-mobility group box 1 activity in central nervous system *** it is commonly known for its detrimental inflammatory effect,high-mobility group box 1 has also been shown to have beneficial pro-regenerative roles in central nervous system *** this narrative review,we provide a brief summary of the history of high-mobility group box 1 research and its characterization as a damage-associated molecular pattern,its downstream receptors,and intracellular signaling pathways,how high-mobility group box 1 exerts the repair-favoring roles in general and in the central nervous system,and clues on how to differentiate the pro-regenerative from the pro-inflammatory *** targeting high-mobility group box 1 in the central nervous system may benefit from differentiating between the two functions rather than overall suppression of high-mobility group box 1.

关键词： central nervous system damage-associated molecular pattern ethyl pyruvate glycyrhizzin high mobility group box 1 inflammation neural stem cells neurodevelopment oligodendrocyte progenitor cells redox status regeneration