检索结果-南通市图书馆

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

特应性皮炎动物模型表皮分化蛋白表达的改变

中国皮肤性病学杂志 2006年第12期20卷 730-733页

作者：杨军 Seung Hun lee 黄晓波 Kenneth R.Feingold Peter M.Elias 史月君蔄茂強大连市皮肤病医院辽宁大连116021 Department of Dermatology Yonsei University School of Medicine Department of Dermatology and Medicine University of California School of Medicine

目的观察特应性皮炎(AD)除真皮T细胞浸润和皮肤增厚外,是否有表皮细胞的分化和增生异常。方法利用反复外涂半抗原的方法制作AD动物模型。在观察该模型皮肤生理学改变的同时,利用免疫组化的方法对表皮分化蛋白的表达和角质形成细胞的增... 详细信息

目的观察特应性皮炎(AD)除真皮T细胞浸润和皮肤增厚外,是否有表皮细胞的分化和增生异常。方法利用反复外涂半抗原的方法制作AD动物模型。在观察该模型皮肤生理学改变的同时,利用免疫组化的方法对表皮分化蛋白的表达和角质形成细胞的增生进行观察。结果AD组角质层的水分含量明显降低;皮肤水分丢失量及pH明显增高。与对照组相比,AD组表皮兜甲蛋白(loricrin)的表达明显减少;在角质层,中间丝相关蛋白(Filaggrin)和外皮蛋白(involucrin)的表达强度稍微减低,但表达部位有明显地改变。正常时仅表达在角质层,而在AD组其颗粒层和棘细胞层均有较强的染色。抗增生细胞核抗体染色显示,AD的表皮增生极其活跃。HE染色显示AD的表皮明显增厚。结论利用反复外涂半抗原的方法可成功制作AD动物模型,AD除皮肤生理的异常外,还伴有表皮增生和分化的异常。

关键词：分化增生透过表皮水分丢失异位性皮炎模型

维普期刊数据库

The inhibitory role of Co2+ on α-glucosidase: inhibition kinetics and molecular dynamics simulation integrating study

在线全文

学校读者我要写书评

暂无评论

The inhibitory role of Co2+ on α-glucosidase: inhibition ki...

生物素蛋白连接酶与抑制剂作用的分子模拟及结合能分析

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Xuan Li Zhi-Rong Lü Dong Shen Yi Zhan Jun-Mo Yang Yong-Doo Park Hai-Meng Zhou Qing Sheng Jinhyuk lee College of Life Sciences Zhejiang Sci-Tech University Zhejiang Provincial Key Laboratory of Applied Enzymology Yangtze Delta Region Institute of Tsinghua University Zhejiang Provincial Key Laboratory of Applied Enzymology Yangtze Delta Region Institute of Tsinghua University School of Life Science Tsinghua University Department of Dermatology Sungkyunkwan University School of Medicine Samsung Medical CenterSeoul 135-710 Korea Korean Bioinformation Center (KOBIC) Korea Research Institute of Bioscience and BiotechnologyDaejeon 305-806 Korea Department of Bioinformatics University of Sciences and Technology Daejeon 305-350 Korea

α-glucosidase(EC 3.***.1.20) is a target enzyme to treat type 2 diabetes mellitus, hence, dueto the clinical relevance, it is important for studying this enzyme’s inhibition. In this study,we investigated Co2+-induced inhibition as well as structural changes of α-glucosidase integratingwith computational simulations. α-glucosidase activity was inhibited by Co2+ ina dose-dependent manner. Co2+ inhibited α-glucosidase in a parabolic non-competitiveinhibition reaction(ki = 0.78 ± 0.08 mM) and directly induced the regional unfolding ofthe enzyme resulting in a slight decrease of hydrophobic surface. The computational simulationsusing molecular dynamics showed that the simulation with Co2+ ions lost the secondarystructure by positioning Co2+ ions near the active site for glucose degradation, implyingthat the Co2+ ions can stimulate the unfolding of the enzyme. Our study revealedthe mechanism of Co2+ ligand binding mediated structural change as well as inhibition ofα-glucosidase, and suggested that Co2+ could act as a potent inhibitor of α-glucosidasefor the treatment of type 2 diabetes *** with computational simulations. α-glucosidase activity was inhibited by Co2+in a dose-dependent manner. Co2+ inhibited α-glucosidase in a parabolic noncompetitiveinhibition reaction(ki = 0.78 ± 0.08 mM) and directly induced the regional unfoldingof the enzyme resulting in a slight decrease of hydrophobic surface. The computationalsimulations using molecular dynamics showed that the simulation with Co2+ ionslost the secondary structure by positioning Co2+ ions near the active site for glucose degradation,implying that the Co2+ ions can stimulate the unfolding of the enzyme. Ourstudy revealed the mechanism of Co2+ ligand binding mediated structural change as wellas inhibition of α-glucosidase, and suggested that Co2+ could act as a potent inhibitor ofα-glucosidase for the treatment of type 2 diabetes mellitus.

关键词： α-glucosidase Co2+ Inhibition kinetics Unfolding Molecular dynamics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

生物素蛋白连接酶与抑制剂作用的分子模拟及结合能分析

Biopathologic features and clinical significance of micrometatasis in the lymph node of early gastric cancer

2019中国化学会第十五届全国计算（机）化学学术会议

作者：贺艳斌 Kwang Jun lee Andrew D.Abell Jingxian Yu 长治医学院药学系 ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP) Department of Chemistry School of Physical Sciences The University of Adelaide

生物素蛋白连接酶(biotin protein ligase, BPL)是一种很有前景的抗生素开发靶点,其抑制剂对临床重要的病原菌如金黄色葡萄球菌和结核分枝杆菌均有疗效。为了找寻对金黄色葡萄球菌有特异选择性,而对人源BPL没有抑制作用的药物分子,人们... 详细信息

生物素蛋白连接酶(biotin protein ligase, BPL)是一种很有前景的抗生素开发靶点,其抑制剂对临床重要的病原菌如金黄色葡萄球菌和结核分枝杆菌均有疗效。为了找寻对金黄色葡萄球菌有特异选择性,而对人源BPL没有抑制作用的药物分子,人们进行了许多努力并报道了系列潜在的PBL抑制剂1。本文从前期研究中选择了7种ki值从0.065?M到33?M以上(对应从化合物1到7)的代表性抑制剂分子1-3(见图1),利用Amber 17.0软件包中的Sander程序进行了分子动力学模拟4。根据PBL与抑制剂相互作用的平衡模拟数据,使用QM/MM-GBSA方法分析了两者的结合能(?G)。结果发现,随着这7种化合物的ki值不断增大,PBL与抑制剂的结合能也相应呈现了增大的趋势(见图1),并且在约-73 kcal/mol时抑制活性出现了明显的拐点,同时结合能和抑制活性之间也存在?G(kcal/mol)=2.86ln(ki(?M))-73.74的线性关系,线性相关系数为0.9798。此外,其余已报道化合物的ki值和?G也基本在这一规律上下波动。这充分说明,当结合能低于-73.74kcal/mol以下时,这一化合物往往具有好的抑制活性,反之,这一抑制剂的活性将较差,不能成为有效的药物候选分子。因此,这一结论为PBL抑制剂的筛选及进一步设计和改造新的抑制剂提供了理论指导。

关键词：生物素蛋白连接酶分子动力学结合能抑制剂

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第2期21卷 667-674页

作者： Min Jung Jo Ji Yeon Park Joon Seon Song Myeong-Cherl Kook Keun Won Ryu Soo-Jeong Cho Jun Ho lee Byung-Ho Nam Eun Kyung Hong Il Ju Choi Young-Woo kim Gastric Cancer Branch Research Institute and HospitalNational Cancer CenterGoyang-si 410-769South Korea Department of Pathology Research Institute and HospitalNational Cancer CenterGoyangsi 410-769South Korea Cancer Registration and Biostatistics Branch and Center for Clinical Trial National Cancer CenterGoyangsi 410-769South Korea

AIM: To evaluate the biopathologic features and clinical significance of nodal micrometastasis(MI) in early gastric cancer(EGC).METHODS: Among 1022 EGC patients who underwent gastrectomy with lymphadenectomy of D1 + β or more from March 2001 to December 2005 at the Korean National Cancer Center, available nodal metastasis was found in 90 p T1N1 patients. Nodal metastasis was confirmed by immunohistochemistry(IHC) with cytokeratin and patients were classified into MI and macrometastasis(MA) groups based on the main tumor burden according to the 6th International Union Against Cancer/American Joint Committee on Cancer staging system; the main tumor burden with a diameter of greater than 0.2 mm but no greater than 2 mm as MI, and greater than 2 mm as MA of the representative metastatic node. Proliferative and apoptotic activities of the primary tumor and the nodal metastasis were measured by IHC with ki-67 and terminal deoxynucleotidyl transferase d UTP nick end labeling, respectively. Biopathologic and clinical features of the patients were analyzed and compared between MI and MA groups. Patients with recurrence were compared with those without recurrence to identify risk factors for ***: Thirty-seven patients showed MI and the other 53 patients revealed MA in the lymph node; the incidence of patients with MI and MA was 41.1% and 58.9%. The main tumor burden was 0.9 and 4.6 mm in the representative metastatic node, respectively. Japanese N2 stations were more frequently involved in MA group(20.9%) than in MI group(10.3%) butthe difference was not statistically different(P = 0.338). Proliferative and apoptotic activities of MI were decreased than those of MA(26.7% vs 40.5%, P = 0.004 and 1.0% vs 3.0%, P < 0.001, respectively). However, nodal MI in the current study showed a relatively high proliferative activity and an equivalent apoptotic activity compared to other cancers in the previously published studies. Recurrence was observed in 6 patients during

关键词： Early gastric cancer Sentinel node biopsy Lymphade

CHARACTERISTICS OF ACUPUNCTURE POINTS COMBINATIONS BASED ON THREE DIFFERENT PATTERN IDENTIFICATION SYSTEM IN THE DONG UI BO GAM

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2014年第3期 305-305页

作者： Won-Mo Jung Tae-Hyung lee Younbyoung Chae Department of Brain Cognitive Engineering Korea University Acupuncture and Meridian Science Research Center Kyung Hee University

Dong Ui Bo Gam literally means "Mirror of Eastern Medicine" and it was on the UNESCO’s Memory of the World Programme in *** is composed of five chapters, and one of them is the Nae Gyeong chapter describing about internal bodily *** of important features of this chapter is that this chapter explains symptoms or diseases based on three different pattern identification *** study intends to review the acupuncture and moxibustion(AM) methods found in Dong Ui Bo Gam with consideration on how the AM methods are utilized based on three pattern identification systems, and to visualize how the features of acupoints are recognized in Dong Ui Bo *** extracted acupoints information from their usage for clinical symptoms or *** describing symptoms and diseases were analyzed using keywords of three pattern identification *** these two processes, we obtained bipartite relationships between acupoints and keywords of three pattern identification *** adjacency matrix presenting the relationship, we defined a characteristic of each acupoint using the keywords by quantifying the relationships with a Term Frequency-Inverse Document Frequency(TF-IDF).We analyzed 728 sentences describing what acupoints can be used to 114 clinical ***, we extracted 496 individual keywords illustrating causes of clinical symptoms or ***, each keyword was categorized into 25 specific concepts of the three pattern identification ***, the characteristics of 115 acupoints were presented by TF-IDF values of 25 *** top 5 acupoints with high TF-IDF were PC6, GB43, BL38, LR8, and CV7 for the internal organs pattern/SP5, ST40, ki1, TE6, and SP2 for the internal or external damages pattern/LU7, ST36, GV20, HT7, and CV12 for the five body constituent elements *** acupoints can be linked to the three pattern identification *** visualization of bipartite relationships between acupoints and t

关键词： CHARACTERISTICS OF ACUPUNCTURE POINTS COMBINATIONS BASED ON THREE DIFFERENT PATTERN IDENTIFICATION SYSTEM IN THE DONG UI BO GAM IDF

数字乳腺断层摄影结合二维乳腺X线摄影与数字乳腺钼靶摄影应用价值的对比:基于人群筛查项目的评估

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

国际医学放射学杂志 2018年第4期 478-478页

作者： S.Hofvind T.Hovda .S.Holen C.I.lee J.Albertsen H.BjФrndal 李亥辰 Oslo and Akershus University College of Applied Sciences

目的比较数字乳腺断层摄影(DBT)和二维乳腺X线摄影(SM),在其与数字化乳腺钼靶(DM)基于一定群体的乳腺X线筛查项目中的应用价值。材料与方法在这项前瞻性群组研究中,于2014—2015年期间纳入通过DBT和SM筛查的37 185名妇女和通过DM筛查的6... 详细信息

目的比较数字乳腺断层摄影(DBT)和二维乳腺X线摄影(SM),在其与数字化乳腺钼靶(DM)基于一定群体的乳腺X线筛查项目中的应用价值。材料与方法在这项前瞻性群组研究中,于2014—2015年期间纳入通过DBT和SM筛查的37 185名妇女和通过DM筛查的61 742名妇女的数据。比较接受DBT和SM筛查和接受DM筛查的女性病人的早期测试指标:包括因乳腺X线异常的召回率、乳腺癌检出率、召回率的阳性预测值、穿刺活检的阳性预测值、病理组织学类型、肿瘤大小、肿瘤分级、淋巴结转移、激素水平、免疫组化ki-67指数以及人表皮生长因子受体-2水平。采用卡方检验、两独立样本t检验和比例检验进行统计学分析。结果DBT和SM筛查的召回率为3.4%,DM筛查的召回率为3.3%(P=0.563)。与DM筛查相比,DBT和SM筛查的癌症检出率明显提高(分别为每1 000例病人筛查出9.4例和6.1例,P<0.001)。每1 000例接受DBT和SM筛查的病人中,检出10 mm或更小肿瘤的比率为3.2/1 000,而接受DM筛查的比率则为1.8/1 000(P<0.001);同时每1 000例接受DBT和SM筛查的病人中,1级肿瘤的检出率为3.3/1 000,而接受DM筛查的病人则为1.4/1 000(P<0.001)。免疫组化分析发现,接受DBT和SM筛查的女性与接受DM筛查的女性之间淋巴结转移率和肿瘤亚型没有显著差异。结论与DM筛查相比,DBT结合SM筛查提高了组织学良恶性肿瘤的检出率。

关键词： SM DM 断层摄影 DBT

软组织肉瘤DWI和DCE-MRI参数与ki-67指数的相关性

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

国际医学放射学杂志 2020年第2期 250-250页

作者： J.H.lee Y.C.Yoon S.W.Seo Y.L.Choi H.S.kim 席海蛟 Department of Radiology Samsung Medical CenterSungkyunkwan University School of Medicine

目的探讨软组织肉瘤(STS)MRI扩散加权成像(DWI)和动态增强成像参数与ki-67标记指数(LI)的相关性。方法伦理委员会批准了该回顾性研究,无需知情同意。

关键词： DWI MRI DCE-MRI ki-67

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

延令草药理作用与毒性研究

武汉医学院学报 1963年第1期 5-10页

作者：胡祟家赵国举李德兴全钰珠方达超李敦周胡文淑赵娇玲曹路敏袁青禄武汉医学院药理学教研组武汉医学院药理学教研组南京医学院药理学教研组重庆医学院药理学教研组

延令草土名头顶一颗珠,简称头珠或一颗珠,系百合科延令草属植物之延令草(Tr-illium Tsuchonoskii Maxim)①此药系鄂西草药中医用以治头痛、眩晕等病症的主要药物②,据坊问均称效果甚好,因此我们认为它可能对高血压症及疼痛有一定疗效,... 详细信息

延令草土名头顶一颗珠,简称头珠或一颗珠,系百合科延令草属植物之延令草(Tr-illium Tsuchonoskii Maxim)①此药系鄂西草药中医用以治头痛、眩晕等病症的主要药物②,据坊问均称效果甚好,因此我们认为它可能对高血压症及疼痛有一定疗效,曾初步时行了一些动物试验,证明有降低血压的作用③。以后继续作进一步的探讨,并按草医的用量试治了一些高血压患者,现将实验方法及给果综合报告如下:

关键词：灌胃给药溶血作用煎剂胆固醇汤剂胆甾醇降压作用提出物腹腔注射

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

陕西省姜片吸虫病疫源地的病原研究

甘肃农大学报 1980年第1期 1-15页

作者：朱学敬张勤李如式魏挺王尔相许绶泰甘肃农业大学兽医系甘肃农业大学兽医系河北省畜牧兽医研究所西北农学院兽医系中国农业科学上海家畜血吸病研究室

1.本文结合陕西省姜片吸虫病疫源地的研究,进行了病原-布氏姜片吸虫〔FaseioIops is bus ki(Lankester,1557)odhner,1902〕的形态和生物学方面的一些工作.2.通过虫体整体标本的观察,较详细地研究了姜片吸虫成虫形态。 3.本文详细观察和... 详细信息

1.本文结合陕西省姜片吸虫病疫源地的研究,进行了病原-布氏姜片吸虫〔FaseioIops is bus ki(Lankester,1557)odhner,1902〕的形态和生物学方面的一些工作.2.通过虫体整体标本的观察,较详细地研究了姜片吸虫成虫形态。 3.本文详细观察和描述了姜片吸虫各期幼虫的形态和发育并与广东的相同资料作了比较讨论,关于成虫的发育观察详另一报告。 4.实验证明:陕西省友中地区在七、八月份室温平均26.32-26.49Oc时,布氏姜片吸虫卵的发育良好,需23天内毛坳成熟,而在室温平均13,21。C时(十一月份),则虫卵停止发育。

关键词：姜片吸虫病平均姜片虫病布氏姜片吸虫布斯克氏姜片吸虫虫卵扁卷螺卵模成卵腔口吸盘性成熟腹吸盘疫源地人工感染陕西省