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超声造影与乳腺癌生物学指标的相关性

中国药业 2024年第S01期33卷 88-91页

作者：赵青杜静李安琪宋少威翟虹新疆医科大学第四附属医院超声科新疆乌鲁木齐830000

目的探讨超声造影征象与乳腺癌分子生物学指标、增殖细胞抗原(ki-67)及组织学分级、分子分型之间的关系,以预测临床预后。方法选取2020年6月至2023年6月在医院手术或穿刺并进行超声造影检查的女性浸润性乳腺癌患者107例(共107个病灶),... 详细信息

目的探讨超声造影征象与乳腺癌分子生物学指标、增殖细胞抗原(ki-67)及组织学分级、分子分型之间的关系,以预测临床预后。方法选取2020年6月至2023年6月在医院手术或穿刺并进行超声造影检查的女性浸润性乳腺癌患者107例(共107个病灶),观察超声造影征象,收集术后乳腺癌分子生物学指标、ki-67及组织学分级、分子分型,分析前者与后三者的相关性。结果人表皮生长因子受体2(HER-2)阳性与阴性组织超声造影不均匀增强、增强后边界不清、向心性增强、增强后范围比较,增殖细胞抗原ki-67阳性与阴性组织超声造影不均匀增强、增强后边界不清、向心性增强、增强后范围比较,不同组织学分级超声造影不均匀增强、增强后边界不清、向心性增强、增强后范围比较,以及不同分子分型超声造影不均匀增强、增强后边界不清、增强后范围比较,差异均有统计学意义(P<0.05)。结论超声造影与乳腺癌分子生物学指标密切相关,对术前开展临床预后评估有提示作用。

关键词：乳腺癌超声造影分子生物学指标组织学分级分子分型

Upregulation of h-TERT and ki-67 in ectopic endometrium is associated with recurrence of endometriosis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第2期23卷 158-163页

作者： Jie LUO Zhimin song Tao ZHANG Ketan CHU Jingyi LI Jianhong ZHOU Jun LIN Department of Gynecology Women's HospitalZhejiang University School of MedicineHangzhou 310006China Department of Obstetrics and Gynecology Women’s HospitalZhejiang University School of MedicineHangzhou 310006China Department of Reproductive Endocrinology Women’s HospitalZhejiang University School of MedicineHangzhou 310006

At present,endometriosis remains a worldwide health burden,with the main symptoms of dysmenorrhea,chronic pelvic pain,and infertility,markedly reducing the quality of life(de Ziegler et al.,2010).Although there is no proof that the disease is associated with high mortality,this disorder can significantly contribute to the deterioration of women’s general well-being(McPeak et al.,2018).

关键词： markedly endometriosis

Cancer stem cell markers CD133 and CD24 correlate with invasiveness and differentiation in colorectal adenocarcinoma

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World Journal of Gastroenterology 2009年第18期15卷 2258-2264页

作者： Dongho Choi Hyo Won Lee Kyung Yul Hur Jae Joon kim Gyeong-Sin Park Si-Hyong Jang Young Soo song ki-Seok Jang Seung Sam Paik Department of Surgery College of MedicineSoonchunhyang University140-743 SeoulSouth Korea Department of Surgery Chi Hang Hospital140-741 SeoulSouth Korea Department of Pathology College of MedicineCatholic University137-040 SeoulSouth Korea Department of Pathology College of MedicineHanyang University133-790 SeoulSouth Korea

AIM:To verify that CD markers are available for detecting cancer stem cell populations and to evaluate their clinical significance in colon ***:Immunohistochemistry for CD133,CD24 and CD44 was performed on the tissue microarray of 523 colorectal *** records were reviewed and clinicopathological analysis was ***:In colorectal adenocarcinoma,128 of 523 cases(24.5%) were positive and 395 cases(75.5%) were negative for CD133 *** hundred and sixty-four of 523 cases(50.5%) were positive and 259 cases(49.5%) were negative for CD24 *** hundred and two of 523 cases(96%) were negative and 21 cases(4%) were positive for CD44 *** clinicopathological analysis,CD133 expression was present more in male patients(P = 0.002) and in advanced T stage cancer(P = 0.024).Correlation between CD24 expression and clinicopathological factors was seen in the degree of differentiation(P = 0.006).Correlation between CD44 expression and clinicopathological factors was seen in the tumor size(P = 0.001).Survival was not significantly related to CD133,CD24 and CD44 ***:CD markers were related to invasiveness and differentiation of colorectal ***,CD expression was not closely related to survival.

关键词： CD133 大肠腺癌分化程度肿瘤大小干细胞标志物 CD44表达临床病理因素侵袭

Wandering small intestinal stromal tumor:A case report

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World Journal of Clinical Cases 2022年第29期10卷 10622-10628页

作者： Jin-Zhan Su Shu-Feng Fan Xia song Lu-Jun Cao Dong-Ying Su Department of Radiology The Second Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310005Zhejiang ProvinceChina Department of Radiology The Hospital of the Zhejiang UniversityHangzhou 310058Zhejiang ProvinceChina

BACKGROUND The occurrence of gastrointestinal stromal tumors(GISTs)in the small intestine is rare,and a case of wandering small intestinal stromal tumor has been rarely reported to *** of this case can help inform future diagnosis and effective *** SUMMARY A 68-year-old patient presented to us with tarry *** tomography showed a mobile tumor moving widely within the abdominal *** the laboratory data showed a low range of red blood cells and an immediate surgery was not indicated,we performed digital subtraction angiography and embolization to achieve *** resection was performed after the patient’s condition *** tumor was successfully removed *** examination revealed submucosal GIST with infarction,which was of intermediate-risk,with mitotic countki67 Hotspots 10%+.The patient was ultimately diagnosed with wandering small intestinal stromal *** When a highly vascularized tumor is clinically encountered in the small intestine,the possibility of stromal tumors should be ***,when the tumor cannot be visualized at its original location,the possibility of tumor migration is considered.

关键词： Wandering tumor Gastrointestinal stromal tumors Small intestine Melena Gastrointestinal hemorrhage Case report

Effect of SMAD7 gene overexpression on TGF-β1-induced profibrotic responses in fibroblasts derived from Peyronie＇s plaque

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Asian Journal of Andrology 2015年第3期17卷 487-492页

作者： Min Ji Choi Kang-Moon song Jin-Mi Park Mi-Hye Kwon ki-Dong Kwon Soo-Hwan Park Dong-Soo Ryu Ji-Kan Ryu Jun-Kyu Suh Department of Urology National Research Center for Sexual Medicine Inha University School of Medicine Incheon 400-711 Korea Department of Urology Changwon Samsung Medical Center Changwon 630-522 Korea Inha Research Institute for Medical Sciences Inha University School of Medicine Incheon 400-711 Korea

Transforming growth factor-β1 （TGF-β1） has been identified as one of the most important fibrogenic cytokines associated with Peyronie＇s disease （PD）. The mothers against decapentaplegic homolog 7 （SMAD7） is an inhibitory Smad protein that blocks TGF-J3 signaling pathway. The aim of this study was to examine the anti-fibrotic effect of the SMAD7 gene in primary fibroblasts derived from human PD plaques. PD fibroblasts were pretreated with the SMAD7 gene and then stimulated with TGF-β1. Treated fibroblasts were used for Western blotting, fluorescent immunocytochemistry, hydroxyproline determination, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assays. Overexpression of the SMAD7 gene inhibited TGF-β1-induced phosphorylation and nuclear translocation of SMAD2 and SMAD3, transdifferentiation of fibroblasts into myofibroblasts, and quashed TGF-β1-induced production of extracellular matrix protein and hydroxyproline. Overexpression of the SMAD7 gene decreased the expression of cyclin D1 （a positive cell cycle regulator） and induced the expression of poly （ADP-ribose） polymerase 1, which is known to terminate Smad-mediated transcription, in PD fibroblasts. These findings suggest that the blocking of the TGF-β pathway by use of SMAD7 may be a promising therapeutic strategy for the treatment of PD.

关键词： cell culture decapentaplegic homolog 7 fibrosis Peyronie's disease transforming growth factor-beta

催化剂作用原理实验设计:碘化钾催化下的L-半胱氨酸氧化反应

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化学教育（中英文） 2024年第8期45卷 36-42页

作者：任成军张程闫双昊赵明李珊珊王健礼游劲松四川大学化学学院四川成都610064

催化剂具有降低反应活化能、加快反应速率等特点。催化化学是物理化学领域的一个重要分支。催化剂的作用原理是催化化学知识体系的基础。为了具象理解该原理,设计了“碘化钾催化下的L-半胱氨酸氧化反应”。该实验通过温度、催化剂2个变... 详细信息

催化剂具有降低反应活化能、加快反应速率等特点。催化化学是物理化学领域的一个重要分支。催化剂的作用原理是催化化学知识体系的基础。为了具象理解该原理,设计了“碘化钾催化下的L-半胱氨酸氧化反应”。该实验通过温度、催化剂2个变量验证了催化剂的作用;通过变色反应检测中间物,理解催化剂改变了反应历程;用原电池电动势测定方法验证了催化剂不改变反应的平衡常数和吉布斯自由能的热力学特性。通过设计创新实验将催化剂作用原理展现得淋漓尽致。该实验将动力学、催化化学和电化学的知识融会贯通,巩固了学生的理论知识水平,锻炼了学生运用知识的能力。设计非金属氧化还原反应电池电动势测定装置,激发了学生的创新能力。

关键词：物理化学动力学催化化学 L-半胱氨酸旋光法变色反应原电池电动势

人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用

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基础医学与临床 2024年第7期44卷 1002-1007页

作者：宋佳赵峰张婷徐静孙静莉陈震宇北部战区总医院妇产科辽宁沈阳110016 中国医科大学干细胞与再生医学研究室辽宁沈阳110122 锦州医科大学妇产科学系辽宁锦州121001

目的探究人羊膜间充质干细胞(hAMSCs)对大鼠子宫瘢痕的修复作用。方法分离培养hAMSCs,选雌性SPF级SD大鼠行子宫壁全层切开术,术中注射hAMSCs,于术后第30天对子宫切开部位进行组织学检查。用ImageJ图像分析软件进行分析比较各组子宫肌层... 详细信息

目的探究人羊膜间充质干细胞(hAMSCs)对大鼠子宫瘢痕的修复作用。方法分离培养hAMSCs,选雌性SPF级SD大鼠行子宫壁全层切开术,术中注射hAMSCs,于术后第30天对子宫切开部位进行组织学检查。用ImageJ图像分析软件进行分析比较各组子宫肌层厚度、纤维化面积百分比。免疫组化法分别检测子宫肌层α平滑肌肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)、肿瘤增殖抗原ki-67(ki-67)阳性面积百分比。结果与磷酸缓冲盐溶液(PBS)组相比,hAMSCs组子宫肌层明显增厚、纤维化面积减少,α-SMA、ki-67阳性表达明显增加(P<0.05),而TGF-β1阳性表达明显减少(P<0.05)。。结论人羊膜间充质干细胞(hAMSCs)可能通过减少瘢痕纤维化的形成、促进子宫瘢痕处平滑肌细胞的增殖等作用机制促进子宫切口组织修复。

关键词：人羊膜间充质干细胞剖宫产子宫瘢痕

TRPV1对肝细胞性肝癌生物学行为的影响及可能的机制预测

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中华疾病控制杂志 2024年第6期28卷 691-701页

作者：韦丽铃刘美良李德远杨钰肖苏洋黎婵华吴思黔宋安华曾小云广西医科大学公共卫生学院南宁530021 广西医科大学公共卫生学院儿少卫生与妇幼保健学教研室南宁530021 广西医科大学公共卫生学院流行病学与卫生统计学教研室南宁530021

目的明确瞬时受体电位香草醛亚家族1(transient receptor potential vanilloid 1,TRPV1)在肝细胞性肝癌(hepatocellular carcinoma,HCC)中的表达及与预后的关系,探讨其对HCC细胞生物学行为的影响。方法利用癌症基因组图谱(the cancer genome atlas,TCGA)、基因型组织表达(genotype-tissue expression,GTEx)及基因表达综合数据库(gene expression omnibus,GEO)验证TRPV1在HCC中的表达及与预后的关系。通过CCK-8实验、划痕实验、Transwell实验、流式细胞术检测TRPV1对HCC细胞增殖、迁移、侵袭和细胞周期的影响,蛋白质印迹法(western blot,WB)检测细胞周期调节蛋白激酶4(cyclin-dependent kinase 4,CDK4)、细胞周期素依赖激酶6(cyclin-dependent kinase 6,CDK6)的表达,裸鼠皮下成瘤实验检测肿瘤增殖能力。最后,构建TRPV1共表达网络并进行富集分析。结果TCGA、GTEx和GEO数据库中,TRPV1在HCC组织中表达水平均下调(均Pki-67蛋白阳性表达水平下调(P=0.010)。TRPV1共表达基因主要参与核糖核酸(ribonucleic acid,RNA)剪接、细胞周期蛋白结合、细胞增殖等过程。结论TRPV1抑制了HCC细胞的增殖、迁移和侵袭能力,并阻滞细胞周期的进程,在HCC中发挥抑癌作用,为HCC预后分析、治疗提供了新方向。

关键词： TRPV1 肝癌增殖迁移细胞周期

Generalized stability criterion for controlling solidification segregation upon twin-roll casting

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Journal of Materials Science & Technology 2023年第3期134卷 163-177页

作者： Pan Wu Yubing Zhang Jiaqi Hu Shaojie song Yong Li Huiyuan Wang Guo Yuan Zhaodong Wang Shizhong Wei Feng Liu State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China Analytical and Testing Center Northwestern Polytechnical UniversityXi’an 710072China State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China State Key Laboratory of Super hard Materials Jilin UniversityChangchun 130012China National Joint Engineering Research Center for Abrasion Control and Molding of Metal Materials Henan University of Science and TechnologyLuoyang 471003China

Macro-and micro-segregation formed upon twin-roll casting(TRC)can be inherited from sub-rapid solid-ification to solid-state transformation,even to plastic deformation,thus deteriorating drastically mechan-ical properties of as-produced thin *** many works focusing mainly on controlling fields of thermal,concentration and convection have been reported,how to control artificially and quantitatively the segregation using a theoretical connection between processing parameters and solidification models,has not been realized,*** it,a systematical framework integrating non-equilibrium dendritic growth and overall solidification kinetics with the TRC parameters,was constructed applying a general-ized stability(GS)conception deduced from transient thermodynamic driving force△G^(t)and transient ki-netic energy barrier Q_(eff)^(t)evolving upon *** from this framework considering synergy of thermodynamics and kinetics(i.e.,thermo-kinetic synergy),a criterion of high△G^(t)-high GS guaranteed that the macro(i.e.,the centerline)and the micro(i.e.,the edge)segregation can be suppressed by in-creasing△G^(t)and GS at the beginning and the ending stage of sub-rapid solidification,*** typical thermo-kinetic combination producing the microstructure can be inherited into the plastic de-formation,as reflected by corresponding strength-ductility *** work realized quantitative controlling of TRC by a theoretical connection between processing parameters and solidification models,where,an optimization for sub-rapid solidification segregation using the GS conception including△G^(t)and Q_(eff)^(t)has been performed.

关键词： Segregation Sub-rapid solidification Generalized stability Twin-roll casting Thermo-kinetics