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The Null Effect of UVC Ceiling Light Exposure on SARS-CoV2

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Health 2020年第11期12卷 1481-1495页

作者： Waheeb E. Alnaser Amjad z. ghanem Naser W. Alnaser Qasim Alaraibi Fatima Alshaikh Department of Physics College of Science University of Bahrain Sakhir Kingdom of Bahrain Public Health Laboratory Ministry of Health Mananama Kingdom of Bahrain Department of Architecture and Interior Design College of Engineering University of Bahrain Kingdom of Bahrain

There has been some extensive research investigating the effect of Far Ultraviolet Radiation (UVC) on SARS and MARS. However, to the best of our knowledge, there have not been any detailed experiments looking at the effect of UVC on COVID-19 (now is called SARS-CoV2). Many researchers in this field believe that UVC destroys SARS-CoV2 because it warps the genetic material of the virus hurdling the viral particles from reproduction. In this paper, we report the result of our novel experiments on the effect of UVC on SARS-CoV2 using a commercially available UVC source, i.e. Krypton Disinfection lighting CM15W12V Series (wavelength of 222 nm), which is sold and marketed for the disinfection of pathogens. The experiments were extended to study the effect of UVC exposure to Bacteria and Fungus. Our experiments show that UVC has no effects on SARS-CoV2 when it is close to the SARS-CoV2 culture plate (4 - 5 cm) or at a distance (2.0 to 2.9 m), i.e. when fixed at the ceiling. This observation is important as the public seems to have the impression that commercial UVC ceiling light can kill SARS-CoV2 while this study has proven the opposite. Moreover, it shows no effect even when the UVC ceiling light is radiating on SARS-CoV2 for overnight. This proves that the intensity of the UVC from these devices is relatively low. However, the UVC light is found to be effective in destroying Bacteria and Fungus (part of pathogens), substantially, in 30 sec, and completely kills them when it’s at 2.9 m (or less) away from them and exposure for one day. This indicates that the UVC light is effective for bacteria disinfection.

关键词： COVID-19 SARS-CoV2 UVC Pathogens UVC Exposure

Do UV LED Devices Immolate SARS-CoV2?

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Health 2020年第11期12卷 1445-1456页

作者： Waheeb E. Alnaser Amjad z. ghanem Naser W. Alnaser Hacene Manaa Qasim Alaraibi Fatima Alshaikh Department of Physics College of Science University of Bahrain Sakhir Kingdom of Bahrain Public Health Laboratory Ministry of Health Manama Kingdom of Bahrain Department of Architecture and Interior Design University of Bahrain Sakhir Kingdom of Bahrain

Some manufactures made UVC LED Strip as disinfection tool against SARS-CoV2. Therefore, three types of commercially UVC lights were used to evaluate their efficiency to warp bacteria and viruses. We tested three commercially available UV lights devices. They were put at 4 to 5 cm to spiked sterile Petri dishes (samples) for 10, 20, 30, and 60 seconds and compared it to control (without UV light exposure). Also, the same three UV LED devices were used on Positive SARS-CoV2 swab samples (used for the Petri dishes). Serial dilutions of the cultured microbes were used for the experiment as follows: 1/10 (high concertation), 1/100, 1/1000, 1/10,000, 1/100,000 (low concertation). All three UV LED devices (DA, DB, DC) were found to have no effects on the pathogens (Bacteria or SARS-CoV2), even to the lowest Bacteria Concentration (1/100,000), when pathogens were exposed to UV radiation for 10, 20, 30, and 60 sec at distance a 4 to 5 cm. One of the manufacturers of these UVC lights (DB) claims that the device is very effective in killing Bacteria and Virus immediately at a 99.93% killing rate (in 20 sec.). This observation was not noticed. False claims may lead to severe spread of SARS-CoV2 as customers may think that the DB was disinfecting, after short exposure, while truly having no effect.

关键词： COVID-19 SARS-CoV2 Pathogens UV Light Devices UVC Bahrain

Determination of Voriconaz.le in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry: Application in Therapeutic Drug Monitoring

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American Journal of Analytical Chemistry 2023年第9期14卷 378-389页

作者： Waleed Alhussaini Ez.eldeen ghanem Magd Alsahly Amani Kurdi Eman Alharbi Imadul Islam Majed Aljeraisy Department of Pharmaceutical Analysis King Abdullah International Medical Research Center King Saud bin Abdulaziz University for Health Sciences Ministry of the National Guard—Health Affairs Riyadh Saudi Arabia Research Office King Abdullah International Medical Research Center King Saud bin Abdulaziz University for Health Sciences Ministry of the National Guard—Health Affairs Riyadh Saudi Arabia

A sensitive, accurate and robust Liquid Chromatography Tandem Mass Spectrometry method has been developed and validated to measure voriconaz.le trough levels in human plasma. The plasma samples were mixed with fluconaz.le as an Internal Standard and directed to protein precipitation and drug extraction. An aliquot of 1 μl was injected into the chromatographic system and separated by the Acquity BEH C18 column at a flow rate of 0.30 ml/min in a gradient mobile phase consisting of acetonitrile, Ultrapure water (UPW), methanol and formic acid. Voriconaz.le was detected by a Triple Quadrupole Detector (TQD) operating on Multiple Reaction Monitoring (MRM) and a positive ion mode Electrospray ioniz.tion (ESI) Q1 mass: 350.1 m/z. Q3 mass: 281.1 m/z. Method linearity of the calibration curve (0.10 - 8.00 μg/ml) indicated a correlation coefficient r ≥ 0.99. The intra and inter-assay accuracy was within 85% - 115% and the intra and inter-assay precision was ≤5.76%. Voriconaz.le recovery percentage was between 97.69 - 119.62%. The method was successively applied in routine voriconaz.le TDM.

关键词： Voriconaz.le Human Plasma Liquid Chromatography Tandem Mass Spectrometry Therapeutic Drug Monitoring

Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Anti-Diabetic Drug Repaglinide in Human Plasma

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American Journal of Analytical Chemistry 2014年第4期5卷 281-290页

作者： Manar K. Fayyad Ez.eldeen H. ghanem Bioanalytical Laboratory International Pharmaceutical Research Center Amman Jordan Department of Chemistry University of Jordan Amman Jordan

A highly sensitive, accurate and rapid analytical method based on reversed-phase liquid chromatography/electrospray ioniz.tion tandem mass spectrometry (RP-LC-ESI-MS/MS) has been developed and validated for the determination of repaglinide in human plasma using cetiriz.ne as an internal standard (IS). The method was validated over a linear range of 0.5 - 100 ng/ml. After addition of IS, analytes were extracted from the plasma samples by liquid-liquid extraction using tert-butyl methyl ether as. The dried residue was reconstituted with 500 μL of mobile phase consisting of water/methanol/acetonitrile (62.5:20:17.5, v/v/v) and 0.2% formic acid. Chromatographic separations were achieved on a C18 analytical column. The analytes were detected with a triple quadrupole mass spectrometer using turbo V? ion spray source with positive ioniz.tion in multiple reaction monitoring (MRM) mode using MRM transitions m/z.453.3 > 162.2 and m/z.389.0 > 201.1 for the drug and IS, respectively. The proposed method was fully validated in terms of linearity, accuracy, precision, specificity, sensitivity, recovery and stability, giving results within the acceptable range. This method was successfully applied for a large number of authentic human plasma samples from a bioequivalence study.

关键词： Repaglinide LC-MS/MS Human Plasma Bioequivalence