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Development of Non-Labeled QCM Biosensor for the Detection of <i>β</i>-Galactosidase: A Comparative Study of Gold and Polystyrene Nanoparticles

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Advances in Nanoparticles 2013年第2期2卷 182-190页

作者： Krishna pal singh Manav Kumar Choudhary Iva Chianella Prashant singh Cranfield Biotechnology Centre Cranfield Health Cranfield University Bedfordshire England Department of Chemistry D.A.V. (PG) College Dehradun H.N.B. Garhwal University Srinagar India Membrane Biophysics & Nanobiosensor Research Laboratory C.B.S H G. B. Pant University of Agriculture& Technology Pantnagar India

The performance of gold and polystyrene nanoparticles was investigated using quartz crystal microbalance (QCM) as sensor platform;β-Galactosidase antibody with corresponding antigen was utilized for the immunoreaction. The development of the immunosensor included: 1) formation of self assembled monolayers on quartz crystals;2a) immobilization of p-aminothiophenol functionalized gold nanoparticles on carboxyl-terminated self assembled monolayer, or 2b) immobilization of polystyrene nanoparticles on crystals modified with p-aminothiophenol self assembled monolayer;3) attachment of monoclonal anti β-Gal on nanoparticles;and 4) detection of target analyte. The nanoparticles used were synthesized in house and characterized by transmission electron microscopy and infrared spectroscopy. The results revealed that antibodies were strongly attached to functionalized gold nanoparticles;the weaker immobilization of antibodies to polystyrene nanoparticles provoked their detachment during antigen detection. When cross reactivity of polystyrene nanoparticles was checked using a different antigen (Brucella), displacement of antibody was not recorded, demonstrating specificity of the reaction. To the best of our knowledge this is the first direct comparison between behaviors of biosensors developed with two commonly used nanoparticles. The results showed that both nanoparticles produced biosensors capable to detect β-Gal. Nevertheless biosensors developed using polystyrene nanoparticles are simpler, cheaper and more eco-friendly than those developed using gold nanoparticles.

关键词： QCM Biosensor β-Galactosidase Gold Nanoparticle Polystyrene Nanoparticle

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SCHS: Smart Cluster Head Selection Scheme for Clustering Algorithms in Wireless Sensor Networks

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Wireless Sensor Network 2012年第11期4卷 273-280页

作者： Vipin pal Girdhari singh Rajender Prasad Yadav Computer Engineering Department Malaviya National Institute of Technology Jaipur India Electronics and Communication Engineering Department Malaviya National Institute of Technology Jaipur India Vice-Chancellor Rajasthan Technical University Kota India

Wireless sensor networks are energy constraint networks. Energy efficiency, to prolong the network for a longer time is critical issue for wireless sensor network protocols. Clustering protocols are energy efficient approaches to extend the lifetime of network. Intra-cluster communication is the main driving factor for energy efficiency of clustering protocols. Intra-cluster energy consumption depends upon the position of cluster head in the cluster. Wrongly positioned clusters head make cluster more energy consuming. In this paper, a simple and efficient cluster head selection scheme is proposed, named Smart Cluster Head Selection (SCHS). It can be implemented with any distributed clustering approach. In SCHS, the area is divided into two parts: border area and inner area. Only inner area nodes are eligible for cluster head role. SCHS reduces the intra-cluster communication distance hence improves the energy efficiency of cluster. The simulation results show that SCHS has significant improvement over LEACH in terms of lifetime of network and data units gathered at base station.

关键词： Wireless Sensor Network Clustering Energy Efficiency Intra-Cluster Communication

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新型高速纺概念生产双收缩丝

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合成纤维 2008年第1期37卷 44-47页

作者： Suprit pal singh 李晔

在PET上一项新的、非常重要的开发被运用于“美感”织物中，这类纤维最初被日本发明家命名为新合纤。双收缩丝（BSY）生产中大部分使用的是低速工艺，通过机械混合2种或更多的不同沸水收缩率的纤维而制得。这种机械混合是通过空气引导... 详细信息

在PET上一项新的、非常重要的开发被运用于“美感”织物中，这类纤维最初被日本发明家命名为新合纤。双收缩丝（BSY）生产中大部分使用的是低速工艺，通过机械混合2种或更多的不同沸水收缩率的纤维而制得。这种机械混合是通过空气引导重复连续交缠混纤，加捻混纤或沿纤维长度方向并捻混纤而实现的。

关键词：沸水收缩率生产高速纺纤维长度机械混合 PET 新合纤发明家

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Synthesis and Characterization of Cadmium Containing Sodium Borate Glasses

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New Journal of Glass and Ceramics 2012年第4期2卷 128-132页

作者： Vandana Sharma Supreet pal singh Gurmel singh Mudahar Kulwant singh Thind Department of Physics Guru Nanak Dev University Amritsar India Department of Physics Punjabi University Patiala India

Glass system (80% B2O3 – (20 – y)% Na2CO3 – y% CdO, where y = 3, 6, 9, 12 and 15 mol%) prepared by meltquenching technique and checked by XRD technique reveals the amorphous nature of these glasses. Increases in density from 2.18 to 2.82 (g/cm3) were observed with an increase in CdO content. Ultraviolet-Visible (UV-VIS) absorption spectra of polished parallel surfaces glass samples showed a decrease in the optical band gap from 3.72 to 3.59 eV with increasing CdO content. The results suggest that CdO content in glass samples plays a role as a network modifier. In addition, a correlation between the results from density, UV-VIS and FTIR measurements indicates that change in the atomic structure is due to the formation of BO4 units.

关键词： Sodium Borate Glasses Optical Band Gap Molar Volume UV-VIS and FTIR Spectroscopy and Bandgap Energy

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Synthesis and Optical Characterization of Silver Doped Sodium Borate Glasses

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New Journal of Glass and Ceramics 2012年第4期2卷 111-115页

Silver doped sodium borate glasses prepared by melt-quenching technique were checked by XRD technique for their amorphous nature. It is observed that the molar volume increases with increasing Ag2O content leading to open struc- ture. Fourier Transform Infrared spectroscopy (FTIR) reveals the formation of BO3 and BO4 groups upon addition of silver oxide as modifier. From the Ultraviolet-Visible (UV-VIS) absorption spectra it is seen that the optical band gap increases with the increase of Ag2O content. Urbach energy is observed between 0.55 - 0.77 eV. The results obtained from molar volume, Fourier Transform Infrared spectroscopy and band gap energy measurements are in agreement with each other and nearly give the similar information about the studied glasses.

关键词： Silver Doped Sodium Borate Glasses Optical Band Gap Molar Volume UV-VIS and FTIR Spectroscopy and Bandgap Energy

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Modified Dual Segment Rectangular Dielectric Resonator Antenna Terminated in a Bio-Medium

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International Journal of Communications, Network and System Sciences 2011年第6期4卷 377-383页

作者： Ravi Kumar Gangwar Surya pal singh Devendra Kumar 不详

In this paper, the simulation studies of the radiation characteristics of modified dual segment rectangular dielectric resonator antenna (DSRDRA) in free space and in presence of bio-medium alongwith the absorbed power distribution in a homogenous bio-medium (muscle layer) in direct contact with the proposed antenna are reported at different WiMAX band frequencies. The specific absorption rate (SAR) distributions in muscle layer for different antenna-to-muscle layer spacings are also presented at resonant frequency of antenna in WiMAX bands. The results of return loss, input VSWR and radiation characteristics of proposed antenna in bio-medium are also compared with results in free space. The simulation study has been carried out using CST Microwave Studio software.

关键词： Modified DSRDRA SAR WiMAX band Radiation Pattern

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HPTLC-MS as a Neoteric Hyphenated Technique for Separation and Forensic Identification of Drugs

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Journal of Analytical Sciences, Methods and Instrumentation 2018年第1期8卷 1-15页

作者： Kanak Lata Verma Manoj Kumar Amar pal singh Regional Forensic Science Laboratory Government of NCT of Delhi New Delhi India Forensic Science Post Graduate Course Department of Anthropology University of Delhi New Delhi India

Drugs are traditionally been identified on basis of chromatographic-spectroscopic hyphenated techniques in instrumental analysis. Gas chromatography (GC) and Liquid chromatography (LC) hyphenated with mass spectroscopy (MS) i.e. GC-MS and LC-MS give reliable and confirmatory results in drugs identification. In the present work the novel hyphenated technique High Performance Thin Layer Chromatography-Mass Spectroscopy (HPTLC-MS) has been used. This technique provides efficient, quick and simple method for identification and separation of Narcotic drugs and psychotropic substances. The drugs under study are Papaverine, Methadone, Cocaine, Ketamine, Caffeine, Codeine, Diazepam, Thebaine, Heroin, Methamphetamine, Carbamazepine, Morphine, Narcotine and Ephedrine. The present study comprising of sixteen drugs has been carried out on CAMAG HPTLC instrument with automatic sampling. Thin layer chromatography (TLC) plates were developed in various solvent systems, scanned under TLC scanner and the results in terms of Retention Factor (Rf value) and UV spectrum (λmax) are presented in the manuscript. Using hyphenated technique of HPTLC-MS (MS 2020 SHIMADZU) spots of these drugs from TLC plate was lifted with CAMAG TLC-MS interface and confirmed by the mass spectrum of the individual drugs by their m/z values thus delivering fast and accurate confirmatory result on the TLC plate.

关键词： Narcotic Drugs Psychotropic Substances HPTLC-MS Chromatography Mass Spectroscopy Hyphenated Techniques Forensic Science

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Synthesis and Biological Activity Study of Novel N1-(4-Hydroxy Benzoyl)-3-Methyl-5-Phenyl-4(N-4-Chlorophenylazo)-1,2-Diazole and Its Derivatives

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Pharmacology & Pharmacy 2017年第1期8卷 1-14页

作者： Sumit Bhatt Gajendra singh Sanjeev Kumar Rachna paliwal Jeet singh Jangwan Chandra pal singh Department of Chemistry SRT Campus Badshahi Thaul Tehri Garhwal University Campus of HNB Garhwal Central University Shrinagar (Uttarakhand) India Sahu Jain P.G. College Najibabad Department of Chemistry MJP Rohilkhand University Bareilly (Uttar Pradesh) India Hindu College Moradabad Department of Chemistry MJP Rohilkhand University Bareilly (Uttar Pradesh) India

A series of sulpha/substituted derivatives of phenyl azo-1,2-diazole have been synthesized and tested as an anti-inflammatory and anti-bacterial activity in mature albino rats hind paw by taking Diclofenac sodium as standard. N1-(4-hydroxy benzoyl)-3-methyl-5-phenyl-4(N-4-chlorophenylazo)-1,2-diazole is synthesized by a two-step process. In the first step, synthesis of N1-4-chlorophenyl hydrazono-1-methyl-3-phenyl propane-1,3-dione by the reciprocal action of 1-methyl-5-phenylpropane-1,3-dione and diazonium salt solution of phenyl-chloride interacts with 4-hydroxybenzoic acid hydrazide to form the final compound. These diazoles, the heterocyclic compounds which contained electron withdrawing groups, were screened for analgesic activity by acetic acid induced writing method, and for anti-inflammatory activity carried on carrageenan-induced paw edema. The synthesized substituted Chlorophenylazo-1,2-diazole nucleus exhibited significant anti-bacterial, anti-cancer, anti-inflammatory activity, muscle relaxing and moderate activity in anti-proliferative studies.

关键词： 1 2-Diazole Diuretic Activity Muscle Relaxing Anti-Inflammatory Analgesic Antibacterial Activity Anti-Proliferative Studies

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Prediction of Antagonistic Activity of <i>β</i>-Carboline and Its Derivatives Using Topological Descriptors

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Open Journal of Applied Sciences 2021年第5期11卷 577-584页

作者： Anil Kumar Soni Gajendra pal singh Vishnu Kumar Sahu Department of Chemistry Shia Post Graduate College Lucknow India Department of Chemistry Bareilly College Bareilly India Department of Chemistry Maharani Lal Kunwari Post Graduate College Balrampur India

Prediction of antagonistic activity of β-carboline and its thirteen derivatives has been made using topological descriptors viz, connectivity index, and kappa shape index of different orders. For evaluation of values of descriptor, molecular modeling and geometry optimization of all the compounds were carried out with CAChe Pro software by opting semiempirical PM3 method using MOPAC 2002. For prediction of activity multiple linear regression analysis (MLR) was performed. MLR analysis has been made by Project Leader Software associated with CAChe by using the above descriptors as independent variables and biological activity as dependent variables. We were performed leave-one-out methods and the result reflected a direct relationship between biological activity and connectivity index of zero order, while indirect relationship with connectivity index of second order and thus connectivity index is a reliable descriptor to predict the biological activity of β-carboline and its various derivatives.

关键词： β–carboline antagonistic activity PM3 connectivity index and kappa shape index

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长丝市场——论当前的旦数细分和应用分支市场

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纺织导报 2009年第7期 67-69页

作者： Suprit pal singh Michael Korobczuk 澳大利亚墨尔本RMIT大学时尚和纺织学院欧瑞康巴马格公司

长丝市场在过去10年已经达到了前所未有的成熟，行业对其已有非常明确的细分。在这篇短文中，笔者将根据现有的市场架构按不同聚合物呈现相关长丝细分市场的一些数据。

关键词：细分市场长丝应用旦数聚合物

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