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Validation of four Helicobacter pylori rapid blood tests in a multi-ethnic Asian population

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World Journal of Gastroenterology 2005年第42期11卷 6681-6683页

作者： Lee-guan Lim khay-guan yeoh Bow Ho Seng-Gee Lim Department of Gastroenterology and Hepatology National University Hospital 5 Lower Kent Ridge Road Singapore 119074 Republic of Singapore Department of Microbiology National University of Singapore Lower Kent Ridge Road Singapore 119260 Republic of Singapore

AIM: To validate the accuracy of four rapid blood tests in the diagnosis of Helicobacter ***: Consecutive dyspeptic patients scheduled for endoscopy at the National University Hospital,Singapore, were interviewed and had blood drawn for serology. The first 109 patients were tested with BM-test (BM), Pyloriset Screen (PS) and QuickVue (QV), and the next 99 subjects were tested with PS and Unigold (UG).Endoscopies were performed blinded to rapid blood test results and biopsies were taken for culture and rapid urease test. Urea breath tests were performed after endoscopies. The rapid blood test results were compared with four reference tests (rapid urease test, culture,serology, and breath test).RESULTS: The study population composed of 208patients (mean age 43.1 years; range 18-73 years; 119males; 174 Chinese). The number of evaluable patientsfor BM, QV, UG and PS were 102, 102, 95, and 197,respectively. The sensitivity and specificity, respectively were: PS 80.2%, 95.8%; UG 55.9%, 100%; QV 43.3%,100%; BM 67.2%, 97.1%.CONCLUSION: The rapid blood test kits showed high specificity and positive predictive value (97-100%), while sensitivity and negative predictive value ranged widely (43%-80% and 47%-73%, respectively). Among test kits, PS showed the best sensitivity (80%), best negative predictive value (73%) and best negative likelihood ratio (0.207). PS had a specificity of 96%, positive predictive value of 97% and positive likelihood ratio of 19.1.

关键词： Helicobacter pylori Rapid blood test

Helicobacter pylori γ-glutamyl transpeptidase:A formidable virulence factor

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World Journal of Gastroenterology 2013年第45期19卷 8203-8210页

作者： Samantha Shi Min Ling khay guan yeoh Bow Ho Department of Microbiology Yong Loo Lin School of Medicine National University of Singapore Singapore 117597 Singapore Department of Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore 117597 Singapore

Helicobacter pylori(***)produce an enzyme known asγ-glutamyl transpeptidase(HpGGT)that is highly conserved and common to all *** has been gaining increasing attention as an important virulence factor of the bacterium,having been demonstrated to be an important colonization factor in several animal models and has also recently been strongly associated with the development of peptic ulcer *** the results of various independent researcher groups,it is clear that HpGGT acts through several pathways to damage gastric epithelial cells including the induction of apoptosis and cell cycle arrest,production of reactive oxygen species leading to DNA damage,promotion of inflammation by increasing cyclooxygenase-2 and interleukin-8 expression,and upregulation of heparin-binding epidermal growth factor-like growth factor resulting in cell survival and *** addition,the potential role of HpGGT in promoting gastric carcinogenesis will also be discussed in this *** from affecting the gastric epithelium,HpGGT also has immunomodulatory actions on host immune cells where it displays an antiproliferative effect on T cells by inducing cell cycle arrest and also works with other *** virulence factors to skew dendritic cells towards a tolerogenic phenotype,possibly contributing to the persistence of the pathogen in the gastric mucosa.

关键词： Helicobacter pylori Gamma-glutamyl transpeptidase Pathogenesis Immunomodulation Carcinogenesis

Complications of endoscopic retrograde cholangiography in the post-MRCP era: A tertiary center experience

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World Journal of Gastroenterology 2005年第33期11卷 5209-5212页

作者： Tze-Zen Ong Jen-Lock Khor Dede-Sutedja Selamat khay-guan yeoh Khek-yu Ho Department of Gastroenterology and Hepatology National University Hospital Singapore

AZM： To evaluate our experience in endoscopic retrograde cholangio-pancreatography （ERCP） in terms of fulfilling the ASGE guidelines in indications, positive findings, and complications in the post-magnetic resonance cholangiopancreatography （MRCP） era. METHODS： Between November 2001 and February 2003, consecutive ERCP cases were prospectively evaluated with regard to the indications, findings, cannulation techniques, devices used during the procedure, sedation given, duration of procedure, and complications. These data were entered in a database for subsequent processing and analysis. RESULTS： Of 336 cases, 21.4% were diagnostic and 78.6% therapeutic ERCR The indications for ERCP fulfilled the ASGE guidelines in 323 cases （96.1%）. Suspected bile duct stone was the most frequent indication （26.8%）, and this was followed by cholangitis （24.4%）, dilated common bile duct （14.9%）, and cholestatic jaundice （23.4%）. Cannulation success rate was 94%. Biliary sphincterotomy was performed in 175 （52.1%） patients. Repeated ERCP was performed on 31.5% of the patients. Overall, the complication rate was 9.8% with 0.3% being procedurerelated mortality. The complications were pancreatitis （5.4%）, bleeding （0.8%）, cholangitis （2.4%） and others （1.5%）. No significant difference was observed between the complication rate and the type of ERCP performed. CONCLUSION： Our study showed that post-ERCP complication rate was comparable with the other large prospective studies and there was no difference in the complication between the diagnostic and therapeutic ERCP.

关键词： Endoscopic retrograde cholangiopancreatogram Complication Pancreatitis

Coexistence of Helicobacter pylori spiral and coccoid forms in experimental mice

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World Journal of Gastroenterology 1998年第6期4卷 30-33页

作者： HUA Jiesong 1, HO Bow 1, ZHENG Pengyuan 1, yEOH khay guan 2, NG Han Chong 1 and LIM Seng Gee 2

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关键词： Helicobacter pylori , antibodies ELISA spiral form coccoid form mouse

Serum IgG response to differentiated antigens of Helicobacter pylori

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World Journal of Gastroenterology 1998年第3期4卷 69-71页

作者： HUA Jie Song 1, KHIN Mar Mar 1, ZHENG Peng yuan 1, yEOH khay guan 2, Ng Han Chong 1 and HO Bow 1

AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHy) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P y. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.

关键词： Helicobacter pylori spiral form coccoid form ELISA Helicobacter pylori infection Helicobacter pylori antigens, bacterial IgG antigens, differentiation antigen antibody reactions stomach ulcer stomach neoplasms gastritis