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Study of the Prevalence of Hepatitis B and Associated Risk Factors in Cotonou (Benin) and Dakar (Senegal)

Open Journal of Immunology 2023年第1期13卷 32-43页

作者： Comlan Jerome Gaston Montcho Pelagie Mougola Bissiengou Léopold Ngor Sene khadidiatou sarr fall Ousseynou Boye Moustapha Mbow Babacar Mbengue Alioune Dieye Maguette Sylla Niang Immunology Department Faculty of Medicine Pharmacy and Odontology Cheikh Anta Diop University of Dakar Dakar Senegal Medical Biology Laboratory of the General Hospital Idrissa Pouye Dakar Senegal

Background: In 2019, the WHO estimated that over 296 million people were living with chronic hepatitis B virus (HBV) infection and over 820,000 deaths attributable to hepatitis B. Most people living with HBV are unaware of their immune status and live in endemic areas. This is the case of Benin and Senegal, which have little data on the disease. Objective: This study aimed to provide epidemiological furthers on hepatitis B in Dakar and Cotonou according to WHO recommendations about “obtaining data for action”. Materials and Methods: Our study took place at the Medical Biology Laboratory of the Idrissa Pouye General Hospital (LBM-HOGIP) in Dakar, Senegal. Participants were selected at the LBM-HOGIP of Dakar or at the LBMs of the health centres of the Archdiocese of Cotonou respectively from November to December 2019 and February to March 2020. All participants were tested for hepatitis B virus antigen (HBsAg) using a microparticle chemiluminescence immunoassay assay. Other risk factors including blood transfusion, haemodialysis, tattooing, cultural or clan scarification, piercing, injecting drug use, unprotected sex and surgical procedures were also investigated. Informed consent was obtained from each participant. The study was approved by the ethics committees in Senegal and Benin. For the biological tests, Excel and IBM SPSS Statistics software were used for the analysis of the results. Results: A total of 470 participants were recruited including 234 in Cotonou and 236 in Dakar. The median age in Cotonou was 29 years with extremes of 10 and 65 years, and 38 years in Dakar with extremes of 6 and 93 years. The prevalence of HBsAg was 12.39% in Cotonou and 19.91% in Dakar. The most affected age groups were 20 - 29 in Dakar and 30 - 39 in Cotonou. Except for piercing, none of the other risk factors considered in our study were found to be associated with HBV transmission in our populations. Conclusion: Our study is hospital-based and revealed high prevalence of HBsAg

关键词： HBsAg Seroprevalence Risk Factors Benin Senegal

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Immunization against Hepatitis B Virus: Serological Status of West African Populations Residing in Cotonou (Benin) and Dakar (Senegal)

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Open Journal of Immunology 2023年第4期13卷 99-108页

作者： Comlan Jerome Gaston Montcho Pelagie Mougola Bissiengou Léopold Ngor Sene khadidiatou sarr fall Ousseynou Boye Moustapha Mbow Babacar Mbengue Alioune Dieye Maguette Sylla Niang Immunology Department Faculty of Medicine Pharmacy and Odontology Cheikh Anta Diop University of Dakar Dakar Fann Senegal Medical Biology Laboratory of the General Hospital Idrissa Pouye Dakar Senegal

Background: According to WHO estimates, by 2022 over 296 million people are living with chronic hepatitis B virus (HBV) infection, and over 820,000 have died from complications. In sub-Saharan African countries such as Benin and Senegal, few research studies have addressed the issue of HBV immunization. Objective: The main objective of this study was to evaluate immunization against the hepatitis B virus in populations residing in Cotonou and Dakar by titrating anti-HBs antibodies (Ab) and detecting total anti-HBc immunoglobulins (Ig). Materials and Methods: This was a prospective, descriptive, analytical study of two West African populations recruited in Dakar at the Laboratory of Medical Biology (LBM) of the General Hospital Idrissa Pouye (HOGIP) and in Cotonou at the LBMs of the health centres of the Cotonou archdiocese. HBsAg-negative patients constituted our study population. The study took place in November-December 2019 for Dakar and February-March 2020 for Cotonou. Anti-HBs antibodies were tested and titrated. In the event of anti-HBs positivity, total anti-HBc was determined. A microparticle chemiluminescence immunoassay was used for marker determination. The detection threshold was 2.50 IU/L for anti-HBs. Excel and IBM SPSS Statistics software were used for data analysis. Subjects’ sociodemographic characteristics were collected using a questionnaire, as was knowledge of their vaccination status. The study was approved by the ethics committees in Benin and Senegal. Results: A total of 394 HBs antigen-negative participants were recruited: 205 in Cotonou and 189 in Dakar. The population was predominantly female, with 65.36% (N = 134) and 57.14% (N = 108) women in Cotonou and Dakar respectively. The median age of participants was 29 years in Cotonou, with extremes of 10 and 65 years, versus 39 years in Dakar, with extremes of 6 and 93 years. Some participants claimed to be unaware of their vaccination status: 33.17% in Cotonou and 56.61% in Dakar. The total p

关键词： HBV Anti-HBs Anti-HBc-Cotonou-Dakar

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Immunological Status to Hepatitis B Virus of Pregnant Women in Dakar, Senegal

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Open Journal of Immunology 2017年第2期7卷 37-44页

作者： Maguette Sylla Niang khadidiatou sarr fall Babacar Mbengue Moustapha Mbow Ndiokhor Nd. Diouf Ousseynou Boye Gora Lo Papa Madieye Guèye Rena Derwiche Amina Sow Sall Tandakha Ndiaye Dieye Alioune Dieye Service of Immunology Faculty of Medicine Pharmacy and Odontology (FMPO) Cheikh Anta Diop University of Dakar (UCAD) Dakar Senegal Laboratory of Medical Biology General Hospital of Grand-Yoff Dakar Senegal Department of Biological and Pharmaceutical Applied Sciences Pharmacy and Odontology (FMPO) Cheikh Anta Diop University of Dakar (UCAD) Dakar Senegal

Objective: Evaluate the immunological status to hepatitis B virus of Senegalese pregnant women by screening HBs antigen. Material and methods: The selection criteria of women were presence at the laboratory for biological exams of pregnancy follow-up. All volunteers for the study were screened for HBs antigen (HBs Ag). Investigation of chronic hepatitis B markers (HBe Ag, anti-HBe, viral quantification) was performed in HBs Ag positive participants. The concentration of anti-HBs antibodies was assessed in HBs Ag negative women. Results: One hundred and fifteen (115) pregnant women were included in the study from July to October 2014. The mean age was 29 ± 6 years, ranging from 16 to 47. The seroprevalence of HBs Ag was 12% and the majority of women (90%) were not vaccinated. Any of the 14 HBs Ag-positive subjects did not express serum HBe Ag, (marker of active viral replication) and all were positive for anti-HBe antibodies. Their viral load (HBV DNA) was undetectable and serum transaminases were normal. The anti-HBs antibodies titrated in HBs-Ag negative women serum revealed that only 46 had protective levels against HBV whilst 55 of them were unprotected.

关键词： HBs Ag Pregnancy Senegal

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Validation of a Capillary Electrophoresis Method for Analyzing Chlorphenamine Maleate-Based Drugs Using the Accuracy Profile Approach

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American Journal of Analytical Chemistry 2016年第5期7卷 452-459页

作者： Serigne Omar sarr Eddy Kevin Senan Ouendo Thierno Mouhamed Wane El Hadji Assane Diop Diop Amadou Serigne Momar Ndiaye Rokhaya Gueye khadidiatou Thiam Aminata sarr Ousmane Niass Djibril fall Bara Ndiaye Serge Rudaz Yérim Mbagnick Diop Laboratory of Analytical Chemistry and Food Sciences Cheikh Anta Diop University Dakar Senegal National Medicines Control Laboratory Ministry of Health Dakar Senegal Laboratory of Pharmaceutical Analytical Chemistry School of Pharmacy Geneva Switzerland Laboratory of Organic and Therapeutic Chemistry Cheikh Anta Diop University Dakar Senegal

This study aimed at validating an analytical method, using the accuracy profile approach, for the assay of chlorphenamine maleate by capillary electrophoresis. The validation was done using concentrations ranging between 75% and 125% of the target concentration of 600 mg/ml. Validation standards were prepared separately in triplicate for each series. Studied validation criteria were selectivity, linearity, trueness, precision (repeatability and intermediate precision), accuracy and limits of detection and quantification. The method was selective, with recoveries ranging between 99.55% and 99.84%. The relative standard deviations of repeatability and intermediate precision were <5%. The accuracy profile confirmed the performance of the assay method between 75% and 100% of the target concentration of 600 mg/ml. The detection and quantification limits were 5 mg/l and 15 mg/l respectively. This ecological and economical method was applied to identify and quantify chlorphenamine maleate in 3 samples of chlorphenamine maleate-based drugs provided by the Senegalese National Medicines Control Laboratory. All analyzed samples were in accordance with official standards.

关键词： Analytical Validation Chlorphenamine Maleate Quality Capillary Electrophoresis CEB Senegal

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