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Establishment of Master Cell Stock and Working Cell Bank of MDCK Lines and Selection and Evaluation of the Lines as Candidate Viral Substrates for Approval production of Combinational Canine Attenuated-live Virus Vaccines

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agricultural sciences in china 2002年第9期1卷 1054-1066页

作者： ZHANG De-li, LI Liu-Jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de( Department of Immunology , School of Basic medical sciences, peking university Health Science Center , beijing 100083 , p. r . china , beijing Institute of preventive medicine, beijing 100071, p. r . china Department of Toxicology at Center for research and Development of animal Healthcare products , Fourth Military medical university , Xi’an 710032 , p. r . china Department of pathology at School of pre-clinical Education , Section of pathology at Xi-Jing Hospital, Institute for Cancer research at china Cancer research Foundation , Fourth Military medical university, Xi’an 710032 , p. r . china key laboratory of preventive veterinary medicine of state ministry of agriculture , college of animal medical sciences and state key laboratory of agricultural biotechnology, college of biological sciences , china agricultural university, beijing 100094 , p.r.china state key laboratory of medical Molecular Biology, School of Basic medical sciences at the peking Union medical Collage /Institute of Basic medical sciences at the Chinese Academy of medical sciences , beijing 100005 , p. r . china ) Department of Immunology School ofBasic Medical Sciences Peking University Health Science Center Beijing 100083 P.R. China beijing Institute of preventive medicine Beijing 100071 P. R. China Department of Toxicology at Center for research and Devel Department of Toxicology at Center for research and Development of animal Healthcare products Fourth Military Medical University Xi'an 710032 P. R. China beijing Institute of preventive medicine Beijing 100071 P. R. China Department of pathology at School of pre-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xi'an 710032 P. R. China key laboratory of preventive veterinary medicine of state ministry of agriculture College of Animal Medical Sciences and State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural UniversityBeijing 100094 P. state key laboratory of medical Molecular Biology School of Basic Medical Sciences at the Peking Union Medical Colloge /Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P. R . China

Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with di-and hy-perploid YB strain of MDCK cell during 17 - 23 passages, with hyper- and hypoploid KA strain of MDCK cell during 6-8 passages, with hypoploid WB strain of MDCK cell on passage 6, with hyper-and hypoploid H strain of MDCK cell during 8-24 passages was 2/24, 6/10, 5/10 and 10/15, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)passages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome kary-otype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. It is thus evident that MDCK cell of WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be approved as substrate for the preparation of attenuated viral vaccines.

关键词： Cell line MDCK Master cell stock Working cell bank Karyotypes Carcinogenesis Tu-morigenicity

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Experimental researches on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotypes

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agricultural sciences in china 2002年第7期1卷 816-827页

作者： ZHANG De-li, LI Liu-jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, YAN Long-fei and FANG Fu-de( Department of Immunology, School of Basic medical sciences , peking university Health Science Center , beijing 100083 , p. r . china beijing Institute of preventive medicine , beijing 100071 , p. r . china Department of Toxicology at Center for research & Development of animal Healthcare products , Fourth Military medical university , Xian 710032 , p. r . china Department of pathology at School of pre-clinical Education , Section of pathology at Xi-Jing Hospital, Institute for Cancer research at china Cancer research Foundation , Fourth Military medical university, Xian 710032 , p. r . china key Open laboratory of preventive veterinary medicine of state ministry of agriculture, college of animal medical sciences & state key laboratory of agricultural biotechnology , college of biological sciences , china agricultural university , beijing 100094 , p.r.china state key laboratory of medical Molecular Biology, School of Basic medical sciences at the peking Union medical Colloge /Institute of Basic medical sciences at the Chinese Academy of medical sciences , beijing 100005 , p. r . china) Department of Immunology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 P. R.China beijing Institute of preventive medicine Beijing 100071P.R.China Department of pathology at School of pre-clinical Education Department of Toxicology at Center for research Development of animal Healthcare products Fourth Military Medical University Xian 710032 P. R . China beijing Institute of preventive medicine Beijing 100071P.R.China Department of pathology at School of pre-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xian 710032 P. R . China key Open laboratory of preventive veterinary medicine of state ministry of agriculture College of Animal Medical Sciences State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural University Beijing 100094 P state key laboratory of medical Molecular Biology School of Basic Medical Sciences at the Peking Union Medical Colloge/Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P.R. China

The chromosomal number variations & structural aberrations of the MDCK cell line, primary feline or canine kidney cell(FKC or CKC) and Hela cell line were investigated and their karyotypes of conventional chromosome bands were analyzed. The carcinogenesis or tumorigenicity testing of these cell lines in about 232 nude mice and for colony formation in soft agarose and for haemagglutination under different concentration of plant lectins of these cells were carried out. Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with tetraploid YA strain of MDCK cell during 20 - 45 passages, with hy-podiploid JB strain of MDCK cell on passage 25, with di-and hypoploid JC strain of MDCK cell during 2-15 passages or with hypoploid M strain of MDCK cell during 9 - 27 passages was 28/58, 1/5, 4/18 and 0/31, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs+ n) and lowest (mcs) passages was not more than 5% - 15% and the structure aberrations was generally 0 - 3% . These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome karyotype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. The repeatedly frozen, thawed and split controls of tumorigenicity-positive cell lines(X strain of Hela, M strain of BHK-21, JA strain of Vero, YA strain of MDCK) have much

关键词： Cell line MDCK Hela Feline or canine kidney cell Karyotypes Carcinogenesis or tuniori-genicity

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Further report on Carcinogenesis or Tumorigenicity ofMDCK Canine Kidney Cell(CKC) Lines and Analysis ofTheir Chromosome Karyotypes

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agricultural sciences in china 2002年第11期1卷 1293-1301页

作者： ZHANG De-li, XIA Geng-tian, HE Xu-yu, GAO Bu-xian ,BAI Xiao-hong ,LI Liu-jin,HUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de(peking university Health Science Center, beijing 100083 ,p. r. china beijing Institute of preventive medicine , beijing 100071 , p.r. china Center for research and Development of animal Healthcare products, Fourth Military medical university, Xi’an 710032, p.r. china Institute for Cancer research at china Cancer research Foundation, Fourth Military medical university, Xi’an 710032, p.r. china key Open laboratory of preventive veterinary medicine, ministry of agriculture/china agricultural university , beijing 100094 , p. r. china state key laboratory of medical Molecular Biology , Institute of Basic medical sciences , beijing 100005 , p. r. china) peking university Health Science Center Beijing 100083P.R.China beijing Institute of preventive medicine Beijing 100071 P.R.China Center for research and Development of animal Healthcare products Fourth Military Medical University Xi'an 710032 P.R. beijing Institute of preventive medicine Beijing 100071 P.R.China Center for research and Development of animal Healthcare products Fourth Military Medical University Xi'an 710032 P.R.China Institute for Cancer research at china Cancer research Foundation Fourth Military Medical University Xi'an 710032 P.R.China key Open laboratory of preventive veterinary medicine Ministry of Agriculture/China Agricultural University Beijing 100094 P.R.China state key laboratory of medical Molecular Biology Institute of Basic Medical Sciences Beijing 100005 P.R.China

Using Hela cell cultures as positive control and primary canine kidney cell (CKC) or feline kidney cell (FKC) cultures purified in vitro on passage 3 as negative control, the tumorigenicity of Madin-Darby canine kidney (MDCK) cells was tested in >273 nude mice, and colony formation in soft agarose and haemag-glutination under different concentration of plant lectins of these cells were carried out at the same time. Subsequently, very low tumorigenicity strains of MDCK line were successfully selected; these were evaluated for the production of canine or feline combination viral vaccines, free of infectious agents, and of known cytoge-netic and tumorigenic. It is thus evident that MDCK cell of M, JB, JC, WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA, YB and KA strains can not be approved as substrate for the preparation of attenuated viral vaccines. The heritable character of these cell sub-lines is comparatively stable, and shows little significant difference between passages.

关键词： Kidney cell line Nude mice Carcinogenesis Tumorigenicity Canine kidney cell (CKC) MDCK

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CANINE OOCYTES CAN BE SpONTANEOUSLY pArTHENOGENErATED AND DEVELOp TO 2-CELL TO 8-CELL STAGE pArTHENOGENErATED EMBrYOS IN COCULTUrE SYSTEM WHICH THEY WErE COCULTUrED WITH CANINE OVIDUCTAL CELLS Or CANINE OVIDUCT EXTrACTS.

CANINE OOCYTES CAN BE SPONTANEOUSLY PARTHENOGENERATED AND DE...

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The 5th Annual Conference of the Asian reproductive biotechnology Society

作者： Lunxue WANG~1 Sheng WANG~1 Jian HOU~1 Yechun pEI~1 Ling LIU~1 Qing LI~1 Yongfu CHEN~1 rongliang HU~2 Xiaorong AN~(1*) 1 state key laboratory of Agrobiotechnology Department of biological sciences china agricultural university beijing 100094 china 2 The Insititue of veterinary medicine Academy of Military medical Science of pLA Changchun 130062 china

＜正＞The objective of this study was to coculture canine oocytes with canine oviductal cells or canine oviduct extracts in modified TCM199 culture medium to improve the MII *** canine oocytes were retrived from the l... 详细信息

关键词： canine oocytes oviductal cells 2-cell 8-cell parthenogenerated

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Minimizing risks of Antimicrobial resistance Development in the Environment from a public One Health perspective

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china CDC weekly 2022年第49期4卷 1105-1109页

作者： Yu Zhang Timothy r.Walsh Yang Wang Jianzhong Shen Min Yang state key laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina Ineos Oxford Institute of Antimicrobial research Department of BiologyUniversity of OxfordOxfordUK beijing key laboratory of Detection Technology for animal-Derived Food Safety College of Veterinary MedicineChina Agricultural UniversityBeijingChina university of Chinese Academy of sciences BeijingChina.

Antimicrobial resistance(AMr)is a globally recognized crisis with meaningful engagement across humans,animals,and the environment as in the One Health *** environment is the potential source,reservoir,and transmission route of AMr,and it plays a key role in AMr development from the One Health *** farming,hospitals,and the pharmaceutical industry are identified as the main emission sources in the *** emissions and determining antimicrobial emission limits are priorities in the containment of environmental AMr *** the perspectives of environmental management and environmental engineering,some important actions to minimize risks of AMr development are summarized,including the recent progress in enhanced hydrolysis pre-treatment technology to control the development of antibiotic resistance genes(ArGs)during biological wastewater *** is desirable to establish a holistic framework to coordinate international actions on the containment of environmental AMr *** establish a community with a shared future for humanity,china should and could play an important role in international cooperation to cope with AMr challenges.

关键词： reservoir summarized globally

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Forecasting the number of species of asexually reproducing fungi(Ascomycota and Basidiomycota)

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Fungal Diversity 2022年第3期114卷 463-490页

作者： Nalin N.Wijayawardene Alan J.L.phillips Diana Santos pereira Dong-Qin Dai AndréAptroot Josiane S.Monteiro Irina S.Druzhinina Feng Cai Xinlei Fan Laura Selbmann Claudia Coleine rafael F.Castañeda-ruiz Martin Kukwa Adam Flakus patricia Oliveira Fiuza paul M.Kirk Kunhiraman C.rajesh Kumar Ilesha S.leperuma Arachchi Nakarin Suwannarach Li-Zhou Tang Teun Boekhout Chen Shuhui Tan r.p.prabath K.Jayasinghe Marco Thines Centre for Yunnan plateau biological resources protection and Utilization College of Biological Resource and Food EngineeringQujing Normal UniversityQujing 655011YunnanPeople’s Republic of China Section of Genetics Institute for Research and Development in Health and Social CareNo:393/3Lily AvenueOff Robert Gunawardane MawathaBattaramulla 10120Sri Lanka Faculdade de Ciências Biosystems and Integrative Sciences Institute(BioISI)Universidade de LisboaCampo Grande1749-016 LisbonPortugal Laboratório de Botânica/Liquenologia Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulAvenida Costa e Silva S/NBairro UniversitárioCampo GrandeMS CEP 79070-900Brazil Coordination of Botany Museu Paraense Emílio GoeldiBelémPA 66077830Brazil Fungal Genomics laboratory(FungiG) College of Resources and Environmental SciencesNanjing Agricultural UniversityWeigang 1Nanjing 210095JiangsuPeople’s Republic of China The key laboratory for Silviculture and Conservation of ministry of Education Beijing Forestry UniversityBeijing 100083People’s Republic of China Department of Ecological and biological sciences University of TusciaViterboItaly Italian National Antarctic Museum(MNA) Mycological SectionGenoaItaly Alejandro de Humboldt’(INIFAT) Instituto de Investigaciones Fundamentales en AgriculturaTropicalSantiago de Las VegasHavanaCuba Department of plant Taxonomy and Nature Conservation Faculty of BiologyUniversity of GdańskWita Stwosza 5980-308 GdańskPoland W.Szafer Institute of Botany Polish Academy of SciencesLubicz 4631-512 KrakowPoland Universidade Federal do rio Grande do Norte(UFrN) Programa de Pós-Graduação em Sistemática e EvoluçãoCentro de BiociênciasCampus UniversitárioAv.Senador Salgado Filho3000Lagoa NovaNatalRN 59078-970Brazil Biodiversity Informatics and Spatial Analysis Jodrell LaboratoryRoyal Botanic GardensKewRichmond TW93DSSurreyUK National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology GroupMACS Agharkar Research InstituteG.G.

Asexually reproducing fungi play a significant role in essential processes in managed and wild ecosystems such as nutrients cycling and multitrophic interactions.A large number of such taxa are among the most notorious plant and animal *** addition,they have a key role in food production,biotechnology and *** without or rare sexual reproduction are distinguished based on their sporulating structures and conidiomata in traditional morphology-based *** number,variation and diversity of asexually reproducing taxa are insufficiently known,even though fungi capable of asexual reproduction may provide an untapped,rich biological resource for future ***,ca.30,000 asexual species belonging to ca.3800 genera have been reported(including 1388 coelomycetous and 2265 hyphomycetous genera).recent reports(2017–2020)reiterate that the number of asexually producing fungi is higher than the number of frequently sexually-reproducing *** the advent of molecular tools and the abandonment of the dual nomenclature system for pleomorphic fungi,priority criteria were established and revisited in the latest outline of fungi and fungus-like ***,species numbers and taxonomic boundaries of pleomorphic taxa and their synanamorphs or synasexual morphs have yet to be *** number of species of speciose genera(***,Aspergillus,Cercospora,Fusarium,phoma and pseudocercospora),cryptic species,species of pleomorphic genera,less studied life modes(such as lichenicolous taxa,taxa from extreme environments)and species from biodiversity-rich areas still need evaluation to achieve more reliable estimates of their *** paper discusses the current knowledge on the matter,with diversity estimates,and potential obstacles in several chapters on(1)speciose genera;(2)pleomorphic genera;(3)cryptic species;(4)well-studied but insufficiently resolved taxa,*** inhabiting species,marine fungi,(5)less studied life modes,e.g.

关键词： Cryptic species DNA sequences Morphology pleomorphism Speciose genera Species identificat

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Knockout of glutathione peroxidase 5 down-regulates the pirNAs in the caput epididymidis of aged mice

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Asian Journal of Andrology 2020年第6期22卷 590-601页

作者： Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin Ma Min-Jie Ni Zi-Mei Ni Yun-Li Guo Zhao-Liang Fei Lan-Tao Gou Qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel r Drevet Yong-Lian Zhang state key laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Genetics reproduction and Development laboratory CNRS UMR 6293-INSERM U1103-Universite Clermont AuvergneClermont-Ferrand 63001France Institute of Neuroscience State Key Laboratory of NeuroscieneeShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China key laboratory of agricultural animal Genetics Breedingand Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of AgricultureHuazhong Agricultural UniversityWuhan 430070China Department of Cellular and Molecular medicine University of CaliforniaSan DiegoLa JollaCA 92093-0651USA Department of Anatomy Histology and EmbryologyShanghai Jiao long University School of MedicineShanghai Key Laboratory of Reproductive MedicineShanghai 200025China department of Cancer Biology Dana-Farber Cancer InstituteBostonMA 02215USA Department of Genetics Harvard Medical SchoolBostonMA 02115USA priority research Centre for reproductive sciences School of Environmental and Life SciencesThe University of NewcastleCallaghanNSW 2308Australia.

The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various *** foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic *** mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GpX5)as a major luminal scavenger in the proximal caput epididymidal ***,the loss of GpX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 *** explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m *** analysis revealed the dysregulation of several thousand epididymal mrNA transcripts,in eluding the downregulation of a subgroup of pirNA pathway gen es,in aged Gpx5^-/-*** agreeme nt with these fin dings,we also observed the loss of pirNAs,which potentially bind to the p-element-induced wimpy testis(plWI)-like proteins pIWIL1 and *** absence of these pirNAs was correlated with the elevated mrNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-***,the oxidative stress response genes tend to have more targeting pirNAs,and many of them were among the top increased genes upon the loss of GpX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic pirNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses.

关键词： epididymis GpX5 oxidative stress pirNA pIWI-interacting rNA small noncoding rNA

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Fungal diversity notes 929-1035:taxonomic and phylogenetic contributions on genera and species of fungi

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Fungal Diversity 2019年第2期95卷 1-273页

作者： rungtiwa phookamsak Kevin D.Hyde rajesh Jeewon D.Jayarama Bhat E.B.Gareth Jones Sajeewa S.N.Maharachchikumbura Olivier raspe Samantha C.Karunarathna Dhanushka N.Wanasinghe Sinang Hongsanan Mingkwan Doilom Danushka S.Tennakoon Alexandre r.Machado Andre L.Firmino Aniket Ghosh Anuruddha Karunarathna Armin Mesic Arun Kumar Dutta Benjarong Thongbai Bandarupalli Devadatha Chada Norphanphoun Chanokned Senwanna Deping Wei Dhandevi pem Frank Kwekucher Ackah Gen-Nuo Wang Hong-Bo Jiang Hugo Madrid Hyang Burm Lee Ishani D.Goonasekara Ishara S.Manawasinghe Ivana Kusan Josep Cano Josepa Gene Junfu Li Kanad Das Krishnendu Acharya K.N.Anil raj K.p.Deepna Latha K.W.Thilini Chethana Mao-Qiang He Margarita Duenas Margita Jadan Maria p.Martin Milan C.Samarakoon Monika C.Dayarathne Mubashar raza Myung Soo park M.Teresa Telleria Napalai Chaiwan Neven Matocec Nimali Ide Silva Olinto L.pereira paras Nath Singh patinjareveettil Manimohan priyanka Uniyal Qiu-Ju Shang rajendra p.Bhatt rekhani H.perera renato Lucio Mendes Alvarenga Sandra Nogal-prata Sanjay K.Singh Santhiti Vadthanarat Seung-Yoon Oh Shi-Ke Huang Shiwali rana Sirinapa Konta Soumitra paloi Subashini C.Jayasiri Sun Jeong Jeon Tahir Mehmood Tatiana Baptista Gibertoni Thuong T.T.Nguyen Upendra Singh Vinodhini Thiyagaraja V.Venkateswara Sarma Wei Dong Xian-Dong Yu Yong-Zhong Lu Young Woon Lim Yun Chen Zdenko Tkalcec Zhi-Feng Zhang Zong-Long Luo Dinushani A.Daranagama Kasun M.Thambugala Saowaluck Tibpromma Erio Camporesi Timur S.Bulgakov Asha J.Dissanayake Indunil C.Senanayake Dong Qin Dai Li-Zhou Tang Sehroon Khan Huang Zhang Itthayakorn promputtha Lei Cai putarak Chomnunti rui-Lin Zhao Saisamorn Lumyong Saranyaphat Boonmee Ting-Chi Wen peter E.Mortimer Jianchu Xu key laboratory for plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Institute of animal Science State Key Laboratory of AnimalNutritionChinese Academy of Agricultural SciencesBeijing 100193People’s Republic of China Center of Excellence in Fungal research Mae Fah Luang UniversityChiang Rai 57100Thailand World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Mushroom research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Rai 50150Thailand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Department of Health sciences Faculty of ScienceUniversity of MauritiusReduitMauritius No.128/1-J Co-Op Azad Housing SocietyCurcaGoa VelhaIndia Department of Crop sciences College of Agricultural and Marine SciencesSultan Qaboos UniversityPO Box 8123 Al KhoudOman Botanic Garden Meise Nieuwelaan 381860 MeiseBelgium Federation Wallonie-Bruxelles Service general de l’Enseignement universitaire et de la Recherche scientifiqueRue A.Lavallee 11080 BrusselsBelgium Shenzhen key laboratory of Microbial Genetic Engineering College of Life Science and OceanographyShenzhen University3688Nanhai AvenueNanshanShenzhen 518060People’s Republic of China Department of plant medicine National Chiayi University300 Syuefu RoadChiayi City 60004Taiwan Departamento de Micologia Universidade Federal de PernambucoRecifePernambucoBrazil Instituto de Ciencias Agrarias Universidade Federal de UberlandiaMonte CarmeloMinas GeraisBrazil Department of Botany&Microbiology H.N.B.Garhwal UniversitySrinagarGarhwal 246174UttarakhandIndia Department of Entomology and plant pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand ruder Boskovic Institute Bijenicka cesta 5410000 ZagrebCroatia Molecular and Applied Mycology and plant pathology laboratory Department of BotanyUniversity of CalcuttaKolkataWest Bengal

This article is the ninth in the series of Fungal Diversity Notes,where 107 taxa distributed in three phyla,nine classes,31 orders and 57 families are described and *** described in the present study include 12 new genera,74 new species,three new combinations,two reference specimens,a re-circumscription of the epitype,and 15 records of sexualasexual morph connections,new hosts and new geographical *** new genera comprise Brunneofusispora,Brunneomurispora,Liua,Lonicericola,Neoeutypella,paratrimmatostroma,parazalerion,proliferophorum,pseudoastrosphaeriellopsis,Septomelanconiella,Velebitea and ***-four new species are Agaricus memnonius,***,Aleurodiscus patagonicus,Amanita flavoalba,***,Amphisphaeria mangrovei,Baorangia major,Bartalinia kunmingensis,Brunneofusispora sinensis,Brunneomurispora lonicerae,Capronia camelliaeyunnanensis,Clavulina thindii,Coniochaeta simbalensis,Conlarium thailandense,Coprinus trigonosporus,Liua muriformis,Cyphellophora filicis,Cytospora ulmicola,Dacrymyces invisibilis,Dictyocheirospora metroxylonis,Distoseptispora thysanolaenae,Emericellopsis koreana,Galiicola baoshanensis,Hygrocybe lucida,Hypoxylon teeravasati,Hyweljonesia indica,Keissleriella caraganae,Lactarius olivaceopallidus,Lactifluus midnapurensis,Lembosia brigadeirensis,Leptosphaeria urticae,Lonicericola hyaloseptispora,Lophiotrema mucilaginosis,Marasmiellus bicoloripes,Marasmius indojasminodorus,Micropeltis phetchaburiensis,Mucor orantomantidis,Murilentithecium lonicerae,Neobambusicola brunnea,Neoeutypella baoshanensis,Neoroussoella heveae,Neosetophoma lonicerae,Ophiobolus malleolus,parabambusicola thysanolaenae,paratrimmatostroma kunmingensis,parazalerion indica,penicillium dokdoense,peroneutypa mangrovei,phaeosphaeria cycadis,phanerochaete australosanguinea,plectosphaerella kunmingensis,plenodomus artemisiae,***,proliferophorum thailandicum,pseudoastrosphaeriellopsis kaveriana,pseudohelicomyces menglunicu

关键词： 86 new taxa Agaricomycetes Ascomycota Basidiomycota Dacrymycetes Dothideomycetes Eurotiomycetes Lecanoromycetes Leotiomycetes Mucoromycetes Mucoromycota pezizomycetes Sordariomycetes Taxonomy

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FungalTraits:a user-friendly traits database of fungi and fungus-like stramenopiles

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Fungal Diversity 2020年第2期107卷 I0001-I0016页

作者： Sergei põlme Kessy Abarenkov rHenrik Nilsson Björn D.Lindahl Karina Engelbrecht Clemmensen Havard Kauserud Nhu Nguyen rasmus Kjøller Scott T.Bates petr Baldrian Tobias Guldberg Frøslev Kristjan Adojaan Alfredo Vizzini Ave Suija Donald pfister Hans-Otto Baral Helle Järv Hugo Madrid Jenni Nordén Jian-Kui Liu Julia pawlowska Kadri põldmaa Kadri pärtel Kadri runnel Karen Hansen Karl-Henrik Larsson Kevin David Hyde Marcelo Sandoval-Denis Matthew E.Smith Merje Toome-Heller Nalin N.Wijayawardene Nelson Menolli Jr Nicole K.reynolds rein Drenkhan Sajeewa S.N.Maharachchikumbura Tatiana B.Gibertoni Thomas Læssøe William Davis Yuri Tokarev Adriana Corrales Adriene Mayra Soares Ahto Agan Alexandre reis Machado Andrés Argüelles-Moyao Andrew Detheridge Angelina de Meiras-Ottoni Annemieke Verbeken Arun Kumar Dutta Bao-Kai Cui C.K.pradeep César Marín Daniel Stanton Daniyal Gohar Dhanushka N.Wanasinghe Eveli Otsing Farzad Aslani Gareth W.Griffith Thorsten H.Lumbsch Hans-peter Grossart Hossein Masigol Ina Timling Inga Hiiesalu Jane Oja John Y.Kupagme József Geml Julieta Alvarez-Manjarrez Kai Ilves Kaire Loit Kalev Adamson Kazuhide Nara Kati Küngas Keilor rojas-Jimenez Krišs Bitenieks Laszlo Irinyi LászlóGNagy Liina Soonvald Li-Wei Zhou Lysett Wagner M.Catherine Aime MaarjaÖpik María Isabel Mujica Martin Metsoja Martin ryberg Martti Vasar Masao Murata Matthew pNelsen Michelle Cleary Milan C.Samarakoon Mingkwan Doilom Mohammad Bahram Niloufar Hagh-Doust Olesya Dulya peter Johnston petr Kohout Qian Chen Qing Tian rajasree Nandi rasekh Amiri rekhani Hansika perera renata dos Santos Chikowski renato L.Mendes-Alvarenga roberto Garibay-Orijel robin Gielen rungtiwa phookamsak ruvishika S.Jayawardena Saleh rahimlou Samantha C.Karunarathna Saowaluck Tibpromma Shawn p.Brown Siim-Kaarel Sepp Sunil Mundra Zhu-Hua Luo Tanay Bose Tanel Vahter Tarquin Netherway Teng Yang Tom May Torda Varga Wei Li Victor rafael Matos Coimbra Virton rodrigo Targino de Oliveira Vitor Xavier de Lima Vladimir S.Mikryukov Yongzhong Lu Yosuke Matsuda Yumiko Miyamoto Urm Institute of Ecology and Earth sciences University of Tartu14A Ravila50411 TartuEstonia Natural History Museum University of Tartu46 Vanemuise Str51003 TartuEstonia Gothenburg Global Biodiversity Centre Box 46140530 GothenburgSweden Department of biological and Environmental sciences University of GothenburgGothenburgSweden Department of Soil and Environment Swedish University of Agricultural SciencesBox 701475007 UppsalaSweden Department of Forest Mycology and plant pathology Swedish University of Agricultural SciencesBox 702675007 UppsalaSweden Section for Genetics and Evolutionary Biology(EvoGene) Department of BiosciencesUniversity of OsloP.O.Box 1066BlindernNO-0316 OsloNorway university of Hawaiʻi at Mānoa 3190 Maile WaySt.John 102HonoluluHIUSA Department of Biology University of CopenhagenUniversitetsparken 152100 CopenhagenØDenmark purdue university Northwest WestvilleIN 46391USA Institute of Microbiology Czech Academy of SciencesVidenska 108314220 Praha 4Czech Republic GLOBE Institute University of CopenhagenØster Farimagsgade 5KH7.2.321353 CopenhagenDenmark Dipartimento di Scienze della Vita e Biologia dei Sistemi Universitàdi TorinoViale Mattioli 2510125 TorinoItaly Department of Organismic and Evolutionary Biology and Farlow Library and Herbarium Harvard University22 Divinity AveCambridgeMA 02138USA Blaihofstr.42 72074 TübingenGermany SYNLAB Estonia Veerenni 53aTallinn 11313Estonia Escuela de Tecnología Médica Universidad Santo TomásLos Carreras 753OsornoChile Centro de Genómica y Bioinformática Universidad MayorCamino La Pirámide 5750HuechurabaSantiagoChile Norwegian Institute for Nature research(NINA) Sognsveien 680855 OsloNorway School of Life Science and Technology University of Electronic Science and Technology of ChinaChengdu 611731People’s Republic of China Evolutionary Biology Faculty of BiologyBiological and Chemical Research CentreUniversity of Warsawul.Zwirki i Wigury 10102-089 WarsawPoland Department of Botany Swedish Museum of

The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental *** the past decades,rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and ***,in spite of the progress of molecular methods,knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains *** order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis *** the information from previous efforts such as FUNGuild and FunFun together with involvement of expert knowledge,we reannotated 10,210 and 151 fungal and Stramenopila genera,*** resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera,designed for rapid functional assignments of environmental *** order to assign the trait states to fungal species hypotheses,the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS *** the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1%dissimilarity threshold.

关键词： Fungal traits Trophic modes Function Guild Bioinformatics High-throughput sequencing Community ecology

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Modes of genetic adaptations underlying functional innovations in the rumen

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Science china(Life sciences) 2021年第1期64卷 1-21页

作者： Xiangyu pan Yudong Cai Zongjun Li Xianqing Chen rasmus Heller Nini Wang Yu Wang Chen Zhao Yong Wang Han Xu Songhai Li Ming Li Cunyuan Li Shengwei Hu Hui Li Kun Wang Lei Chen Bin Wei Zhuqing Zheng Weiwei Fu Yue Yang Tingting Zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv Wei Sun Xinyu Li Shisheng Huang Lixiang Liu Shengyong Mao Wenqing Liu Jinlian Hua Zhipeng Li Guojie Zhang Yulin Chen Xihong Wang Qiang Qiu Brian pDalrymple Wen Wang Yu Jiang key laboratory of animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China School of Ecology and Environment Northwestern Polytechnical UniversityXi’an710072China Section for Computational and rNA Biology Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark university of Chinese Academy of sciences Beijing100049China Center for Excellence in animal Evolution and Genetics Chinese Academy of SciencesKunming650223China CEMS NCMISMDISAcademy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing100080China Marine Mammal and Marine Bioacoustics laboratory Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanya572000China college of Life sciences Shihezi UniversityShihezi832003China state key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Animal Science and TechnologyGuangxi UniversityNanning530005China college of animal Science and Technology Yangzhou UniversityYangzhou225009China Joint International research laboratory of agriculture and Agri-product Safety of ministry of Education of china Yangzhou UniversityYangzhou225009China key laboratory for Major Obstetric Diseases of Guangdong province The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510150China School of Life Science and Technology Shanghai Tech UniversityShanghai201210China college of animal Science and Technology Nanjing Agricultural UniversityNanjing210095China college of veterinary medicine Shaanxi Centre of Stem Cells Engineering&TechnologyNorthwest A&F UniversityYangling712100China Department of Special Economic animal Nutrition and Feed Science Institute of Special Animal and Plant SciencesChinese Academy of Agricultural SciencesChangchun130112China Section for Ecology and Evolution Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark china National GeneBank BGI-ShenzhenShen

The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant *** analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional *** identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other *** genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen *** modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory *** validated that the key ketogenesis rate-limiting gene(HMGCS2)with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other *** newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community ***,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene *** results greatly improve our understanding of rumen evolution and organ evo-devo in general.

关键词： rumen innovations comparative transcriptomics comparative genomics evo-devo evolution of organs

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