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Acta Pharmacologica Sinica 2006年第10期27卷 1340-1345页

作者： Wen-jing LI Teng-ke WANG Xian WANG~2.Department of Physiology,School of Basic Medical sciences,peking {2.,key laboratory of molecular cardiovascular science,ministry of education,beijing 100083.{12. Department of Physiology School of Basic Medical Sciences Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing 100083 China

Aim:Our previous data have shown that type Ⅱ alveolar epithelial(AEⅡ)cellsexpress neuropeptide calcitonin gene-related peptide(CGRP),and that pro-in-flammatory factor interleukin 1-β(IL-1β)induces CGRP secretion in the A549 hu-man AEⅡ cell *** the present study,we investigated the effect of endogenousand exogenous CGRP on IL-1β-induced chemokine interleukin-8(IL-8)***:We used enzyme-linked immunosorbent assay(ELISA)and RT-PCR todetect IL-8 protein and mRNA levels,respectively,siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene,respectively,and chemiluminescence assay was used to detect reactive oxygenspecies(ROS)***:CGRP-1 receptor antagonist hCGRP8-37(0.1-1nmol·L-1)greatly amplified IL-1β-induced IL-8 *** inhibition of CGRPexpression by siRNA significantly increased IL-8 secretion upon IL-1β ***,cell clones stably transfected with CGRP showed significantly inhibitedmRNA and protein levels of IL-8 induced by 1L-1β.Conclusion:These data implythat AEⅡ cell-derived CGRP suppress IL-1β-induced IL-8 secretion in an autocrine/paracrine *** investigation showed that CGRP attenuated IL-1β-arousedROS formation,which is an early indication of pro-inflammatory factor signaling.

关键词： type Ⅱ alveolar epithelial cells interleukinl interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species

Petrogenesis of Songshugou dunite body in the Qinling orogenic belt, Central china: Constraints from geochemistry and melt inclusions

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science china Earth sciences 2005年第8期48卷 1146-1157页

作者： SU Li1,2. SONG Shuguang3 & ZHOU Dingwu1 1. key laboratory of Continental Dynamics of the {2. of education, Department of Geology, Northwestern university, Xi’an 710069, china 2. The Geological Lab center, china university of Geosciences (beijing), beijing 100083. china 3. key laboratory of Orogenic Belts of the {2. of education, Department of Geology, peking university, beijing 100871, china 1. key laboratory of Continental Dynamics of the {2. of education Department of Geology Northwestern University 710069 Xi’an China 2. The Geological Lab center China University of Geosciences 100083 (Beijing)Beijing China 3. key laboratory of Orogenic Belts of the {2. of education Department of Geology Peking University 100871 Beijing China

The Songshugou dunite body, which occupies an area of about 2. km2. is the largest ultramafic massif in the eastern Qinling orogenic belt, Central china. The major compo-nent of this body is dunitic rocks including mylonitic dunite and coarse-grained dunite; they oc-cupy about 95 vol% of the total body. Petrography, mineral composition, major and trace ele-ments and primitive melt inclusions have been investigated in this paper; all revealed that this dunite body is the product of melt-rock interaction by porous percolation flow. In comparison with dunite sills or veins in harzburgite from the basal part of Oman ophiolite, this dunite body is characterized by lower contents of Al2.3, CaO and HREE but higher content of highly incom-patible elements and Zr and Hf. The LREE enriched patterns and primitive mantle normalized spidergrams of trace elements are also different from the Oman dunite. Combining with melt in-clusions observed in olivines, we conclude that this dunite body is the product of large- scale porous percolation flow of high-MgO melts within depleted mantle peridotites. The high-MgO magma, in essence, was most probably produced at the hot head of an upwelling mantle plume. The occurrence of the Songshugou dunite body is closely associated with the activity of mantle super-plume in Neo-Proterozoic era in the Yangtze Craton.

关键词： Songshugou dunite body, geochemistry, melt inclusion, mantle plume, Qinling orogenic belt.

Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats

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Acta Pharmacologica Sinica 2006年第3期27卷 299-306页

作者： Sheng-ying WU~(2.6) Chun-shui PAN~3 Bin GENG~3 Jing ZHAO~3 Fang YU~3 Yong-zheng PANG~3 Chao-shu TANG~4 Yong-fen QI~(3,4,5) ~2.nstitute of cardiovascular Disease,peking {2. First Hospital.beijing 100034 ~3Department of Physiology and Pathophysiology,peking {2. health science center,beijing 100083.china,~4{12. laboratory of molecular cardiovascular science,ministry of education,beijing 100083.china 2.nstitute of cardiovascular Disease Peking University First Hospital Beijing 100034 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 4key laboratory of {2. cardiovascular science Ministry of Education Beijing 100083 China 4key laboratory of {2. cardiovascular science Ministry of Education Beijing 100083 China

Aim:To investigate the role of the endogenous cystathionine γ-synthase(CSE)/hydrogen sulfide(H2.)pathway in vascular calcification in ***:A ratvascular calcification model was established by administration of vitamin D3 plusnicotine(VDN).The amount of CSE and osteopontin(OPN)mRNA was deter-mined by using semi-quantitative reverse-transcription polymerase chain *** calcium content,45Ca2.accumulation and alkaline phosphatase(ALP)activitywere measured.H,S production and CSE activity were ***:yonKossa staining produced strong positive black/brown staining in areas amongthe elastic fibers of the medial layer in the calcified *** calcium content,45Ca2.accumulation and ALP activity in calcified arteries increased by 6.77-,1.42.,and 1.87-fold,respectively,compared with *** expression of the OPNgene was upregulated(P2.uptake and ALP activity in the VDN plus NaHSgroup was lower than that in the VDN *** content of calcium and45Ca2.accumulation and activity of ALP in the aorta were 34.8%,40.75% and 63.5% lowerin the low-dosage NariS group than in the VDN group,respectively(P2.and activity of ALP was 83.9%,37.8 % and 46.2. lower in the aorta in the high-dosage NariS group than in theVDN group,respectively(P2. can ameliorate vascular calcification,suggesting that the H2./CSE pathway plays a regulatory role in the pathogenesis of vascular calcification.

关键词： hydrogen sulfide cystathionine-lyase vascular calcification mRNA

Composition of sandstone and heavy minerals implies the provenance of Kuqa Depression in Jurassic, Tarim basin, china

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Progress in Natural science:Materials International 2005年第7期15卷 633-640页

作者： WU Chaodong, LIN Changsong, SHEN Yanping and FENG Xue ( key laboratory of Orogenic Belts and Crustal Evolution, {2. of education, peking university, beijing 100871, china Department of Resources, china university of Geosciences, beijing 100083. china) key laboratory of Orogenic Belts and Crustal Evolution Ministry of Education Peking University Beijing 100871 China. Department of Resources China University of Geosciences Beijing 100083 China

The petrological characteristics and heavy mineral assembly of the Jurassic System in Kuqa Basin are consistent with the tectonic evolution, which is the major factor controlling the filling process of Kuqa Basin. Controlled by the source areas at the both sides of the Basin, the matrixes are dominated by sedimentary rock, high-grade metamorphic rock, acid magmatic rock, and the highest heavy mineral assemblage of zircon-garnet-magnetite in the content. According to heavy mineral indexes, the Jurassic strata can be classified into three rhythmic units of heavy mineral. The lower sequence contains abundant heavy minerals and relatively high content of detritus and its matrixes primarily came form the recycling orogen. The assemblage of "zircon-garnet-magnetite" indicates that the sedimentary area in the early evolution period of the basin is relatively near the provenance. The middle sequence is different from the lower sequence in the heavy mineral assemblage and has the different characteristics from that of source rock. The heavy mineral assemblage is "garnet-zircon" with the highest content of garnet and its main matrices are the high-grade metamorphic rock, acid magmatic rock and hydrothermal vein at the northeastern edge of the basin. The middle and upper sequences are characterized by the clast provenance, which is primarily from the recycling orogen at the northern edge of the basin and secondarily from the southern uplift. As a result of the two provenances, the strata with apparent changes of heavy mineral assemblage and index better coincides with the interfaces of the secondary strata sequences, which reflects the denudation history and the filling process of the orogen at the northern edge of the basin.

关键词： sandstone components provenance analysis filling sequence tectonic evolution.

Kinematics and dynamics of the Mesozoic orogeny and late-orogenic extensional collapse in the Sino-Mongolian border areas

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science china Earth sciences 2005年第7期48卷 849-862页

作者： ZHENG Yadong 1 & WANG Tao2.1. key laboratory of Orogenic Belts and Crustal Evolution ({2. university), ministry of education, beijing 100871, china 2. Institute of Geology, Chinese Academy of Geological sciences, beijing 100037, china key laboratory of Orogenic Belts and Crustal Evolution ({2. university) Ministry of Education Beijing 100871 China Institute of Geology Chinese Academy of Geological Sciences Beijinq 100037 China

The Sino-Mongolian border areas underwent two important tectonic events during Mesozoic time after late Paleozoic orogeny: a late Triassic to earlier Jurassic contractional event that resulted in a large-scale south-vergent thrust during the orogeny and a late Jurassic-earlier Cretaceous extensional event in a north-south direction that formed a metamorphic core complex. The kinematic and dynamic analyses show that the thrust sheet moved southwards with a kinematic vorticity number of ca. -0.10 and sub-horizontal maximum compressive stress axis that belongs to a contraction-thickening shear. The upper plate of the late-orogenic detachment relatively moved in a 165°direction. The average kinematic vorticity in its earlier stage was 0.74 that belongs to simple shear dominated shearing and related to the maximum compressive stress axes dipping at ～66°, while the later average kinematic vorticity was ～0.55°that belongs to pure shear dominated shearing with sub-vertical maximum compressive stress axes. This suggests that the thrusting led to the crust thickened and the lower plate rocks that were originally located in the upper crust depressed through a brittle-ductile transition zone into the lower crust and became warmer. The heated rocks trended to uplift since their increasing volume and decreasing density while the loading of the upper-plate rocks increased due to the structural thickening. Under the combined effect of the loading and the thermal-uplifting, the ductile shear zone in between increased in its component of vertical pure shear. Once its pure-shear component exceeded its simple-shear one the ductile shear zone became an extension-thinned shear zone. This progressive transitional process reflects internal and essential temporal and spatial relationships: the extensional factor nucleated during the crust thickening by thrusting and increase of the extensional factor finally led to late-orogenic collapse.

关键词： Mesozoic thrusting,late-orogenic collapse,kinematic vorticity number,stress state.

Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart

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science china(Life sciences) 2009年第11期52卷 1003-1010页

作者： WANG Jun1,2. BAI Ling1,2. LI Jing3, SUN ChaoFeng1,2. ZHAO Jin1, CUI ChangCong1,2. HAN Ke1,2. LIU Yu1,2. ZHUO XiaoZhen1,2. WANG TingZhong1,2. LIU Ping1,2. FAN FenLing1,2. GUAN YouFei3 & MA AiQun1,2.1 Department of Cardiology, First affiliated hospital of medical college of Xi’an Jiaotong university, Xi’an 710061, china 2.Ion Channel Disease {2., key {2. of Environment and Genes Related to Diseases of education ministry, Xi’an 710061, china 3 Department of Physiology and Pathophysiology key {2. of molecular cardiovascular {12.s of the ministry of education, peking university health science center, beijing 100083. china 1. Department of Cardiology First affiliated hospital of medical college of Xi’an Jiaotong University Xi’an 710061 China 2. Ion Channel Disease laboratory Key Laboratory of Environment and Genes Related to Diseases of Education Ministry Xi’an 710061 China 3. Department of Physiology and Pathophysiology key laboratory of {2. cardiovascular sciences of the ministry of education Peking University Health Science Center Beijing 100083 China

This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 2. proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury.

关键词： mitochondria heart failure proteome two-dimensional electrophoresis energy metabolism

Effects of Cu^(2.) on characteristic of SV currents

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science china Chemistry 2006年第6期49卷 560-564页

作者： ZHANG Liping1,2.& YANG Pin1 1. key laboratory of Chemical Biology and {2. Engineering of ministry of education, Institute of {2. science, Shanxi university, Taiyuan 030006, china 2. Institute of Environment science, Taiyuan university of science and Technology, Taiyuan 03002., china 1. key laboratory of Chemical Biology and {2. Engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China 2. Institute of Environment science Taiyuan University of Science and Technology Taiyuan 030024 China

Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu2. on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu2. in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu2. significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu2.. These results show that there may be some Cu2. binding sites on SV channels and binding to which can change SV current’s characteristic.

关键词： the patch-clamp technique, vacuolar, Cu2., SV channel.

Metal-amyloid-β peptide interactions: a preliminary investigation of molecular mechanisms for Alzheimer’s disease

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science china Chemistry 2007年第4期50卷 453-467页

作者： JIAO Yong & YANG Pin key laboratory of Chemical Biology and {2. Engineering of ministry of education, Institute of {2. science, Shanxi university, Taiyuan 030006, china 1. key laboratory of Chemical Biology and {2. Engineering of ministry of education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Although humans have spent exactly 100 years combating Alzheimer’s disease (AD), the molecular mechanisms of AD remain unclear. Owing to the rapid growth of the oldest age groups of the popula-tion and the continuous increase of the incidence of AD, it has become one of the crucial problems to modern sciences. It would be impossible to prevent or reverse AD at the root without elucidating its molecular mechanisms. From the point of view of metal-amyloid-β peptide (Aβ) interactions, we review the molecular mechanisms of AD, mainly including Cu2. and Zn2. inducing the aggregation of Aβ, cata-lysing the production of active oxygen species from Aβ, as well as interacting with the ion-channel-like structures of Aβ. Moreover, the development of therapeutic drugs on the basis of metal-Aβ interactions is also briefly introduced. With the increasingly rapid progress of the molecular mechanisms of AD, we are now entering a new dawn that promises the delivery of revolutionary developments for the control of dementias.

关键词： Alzheimer’s disease (AD) amyloid-β peptide Cu2. Zn2. molecular mechanisms aggregation active oxygen species ion channel

Magnetism induced by nonmagnetic dopants in zinc-blende SiC: First-principle calculations

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science china(Physics,Mechanics & Astronomy) 2010年第1期53卷 1-10页

作者： LIU ZhaoQing & NI Jun Department of Physics and key laboratory of Atomic and {2. Nanoscience (ministry of education), Tsinghua university, beijing 100084, china 1. Department of Physics and key laboratory of Atomic and {2. Nanoscience (ministry of education) Tsinghua University Beijing 100084 China

Magnetism induced by the nonmagnetic dopants in the zinc-blende SiC (3C-SiC) is investigated by first-principle calculations. The atoms of the first 2. elements in the periodic table except inert gas are used to replace either Si or C atoms as dopants. We find that some nonmagnetic substitutional dopants (mainly the Group IA, Group IIA, Group IIIB, and Group VIIB elements) prefer the spin-polarized ground states with local magnetic moments. In general, the condition for obtaining the local magnetic moments and the magnetic ground state requires that the dopants are p-type and have large electronegativity difference from the neighboring host atoms. The magnetic moments can be tuned over a range between 1 μ B and 3 μ B by doping with the nonmagnetic elements. The nearest-neighbor exchange couplings J 0 between the local magnetic moments are quite large and the codoping method is proposed to increase the dopant concentration. These imply that the nonmagnetic doping in SiC may exhibit collective magnetism. Moreover, the Group IIA Mg and Ca atoms substituting the preferred Si atoms favor the ferromagnetic ground states with the half-metallic electronic properties, which suggests that Mg or Ca substitutional doping on the Si sites in SiC could be a potential route to fabricating the diluted magnetic semiconductors.

关键词： nonmagnetic dopant magnetism SiC diluted magnetic semiconductor first-principle calculation

A malware detection model based on a negative selection algorithm with penalty factor

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science china(Information sciences) 2010年第12期53卷 2461-2471页

作者： ZHANG PengTao 1,2., WANG Wei 1,2.& TAN Ying 1,2.1 Department of Machine Intelligence, School of Electronics Engineering and Computer science, peking {2., beijing 100871, china 2.key laboratory of Machine Perception, ministry of education, peking {2., beijing 100871, china 1. Department of Machine Intelligence School of Electronics Engineering and Computer Science Peking University Beijing 100871 China2. Key Laboratory of Machine Perception Ministry of Education Peking University Beijing 100871 China

A malware detection model based on a negative selection algorithm with penalty factor (NSAPF) is proposed in this paper. This model extracts a malware instruction library (MIL), containing instructions that tend to appear in malware, through deep instruction analysis with respect to instruction frequency and file frequency. From the MIL, the proposed model creates a malware candidate signature library (MCSL) and a benign program malware-like signature library (BPMSL) by splitting programs orderly into various short bit strings. Depending on whether a signature matches "self", the NSAPF further divides the MCSL into two malware detection signature libraries (MDSL1 and MDSL2., and uses these as a two-dimensional reference for detecting suspicious programs. The model classifies suspicious programs as malware and benign programs by matching values of the suspicious programs with MDSL1 and MDSL2. Introduction of a penalty factor C in the negative selection algorithm enables this model to overcome the drawback of traditional negative selection algorithms in defining the harmfulness of "self" and "nonself", and focus on the harmfulness of the code, thus greatly improving the effectiveness of the model and also enabling the model to satisfy the different requirements of users in terms of true positive and false positive rates. Experimental results confirm that the proposed model achieves a better true positive rate on completely unknown malware and a better generalization ability while keeping a low false positive rate. The model can balance and adjust the true positive and false positive rates by adjusting the penalty factor C to achieve better performance.

关键词： penalty factor negative selection algorithm signature extraction artificial immune system mal- ware detection