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The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.

Journal of Integrative Plant Biology 2024年第8期66卷 1735-1751页

作者： Weizhi He Hang Liu Zhangkuanyu Wu Qing Miao Xinyi Hu Xin Yan Hangyu Wen Yaojie Zhang Xueqing Fu Li Ren kexuan tang Ling Li Frontiers Science Center for Transformative Molecules Joint International ResearchLaboratory of Metabolic and Developmental SciencesKey Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Institute for Agri-Food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin production,but the underlying regulatory mechanism remains *** this study,we demonstrate that ELONGATED HYPOCOTYL5(HY5),a central transcription factor in the light signaling pathway,cannot promote artemisinin biosynthesis on its own,as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their *** analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with ***21 showed a trichome-specific expression ***,the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1,AaMYB108,and AaORA,encoding positive regulators of artemisinin ***,AaHY5 and AaBBX21 physically interacted with the *** E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1).In the dark,AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex,explaining the enhanced production of artemisinin upon light *** study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.

关键词： AaBBX21 AaHY5 AaCOP1 Artemisia annua L. artemisinin biosynthesis light

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Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation

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Plant Communications 2024年第3期5卷 31-46页

作者： Junfeng Cao Zhiwen Chen Luyao Wang Ning Yan Jialing Lin Lipan Hou Yongyan Zhao Chaochen Huang Tingting Wen Chenyi Li Saeed ur Rahman Zehui Liu Jun Qiao Jianguo Zhao Jie Wang Yannan Shi Wei Qin Tong Si Yuliang Wang kexuan tang Frontiers Science Center for Transformative Molecules Joint International Research Laboratory of Metabolic and Developmental SciencesPlant Biotechnology Research CenterFudan-SJTU Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Engineering Research Center of Coal-based Ecological Carbon Sequestration Technology of the Ministry of Education Key Laboratory of Graphene Forestry Application of National Forest and Grass AdministrationShanxi Datong UniversityDatong 037009China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Hainan Institute Zhejiang UniversityYongyou Industry ParkYazhou Bay Sci-Tech CitySanya 572000China College of Agriculture and Biotechnology Zhejiang UniversityHangzhou 310058China Shandong Provincial Key Laboratory of Dryland Farming Technology College of AgronomyQingdao Agricultural UniversityQingdao 266109China Institute of Millet Crops Hebei Academy of Agriculture&Forestry Sciences/Hebei Branch of China National Sorghum Improvement CenterShijiazhuang 050035China

We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological *** growth and biomass were promoted when *** was cultivated with low concentrations of graphene,an effect which was attributed to a 1.4-fold increase in nitrogen uptake,a 15%–22%increase in chlorophyllfluorescence,and greater abun-dance of carbon cycling–related *** to 10 or 20 mg/L graphene resulted in a�60%increase in H2O2,and graphene could act as a catalyst accelerator,leading to a 9-fold increase in catalase(CAT)ac-tivity in vitro and thereby maintaining reactive oxygen species(ROS)***,graphene exposure led to an 80%increase in the density of glandular secreting trichomes(GSTs),in which artemisinin is biosynthesized and *** contributed to a 5%increase in artemisinin content inmature ***-estingly,expression of miR828 was reduced by both graphene and H2O2 treatments,resulting in induction of its target gene AaMYB17,a positive regulator of GST *** molecular and genetic assays showed that graphene-induced H2O2 inhibits micro-RNA(miRNA)biogenesis through Dicers and regulates the miR828–AaMYB17 module,thus affecting GST *** results suggest that graphene may contribute to yield improvement in *** via dynamic physiological processes together with miRNA regulation,and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.

关键词： Artemisia annua artemisinin glandular secreting trichomes GSTs miRNA graphene

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Current Opinions on the Functions of Tocopherol Based on the Genetic Manipulation of Tocopherol Biosynthesis in Plants

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Journal of Integrative Plant Biology 2008年第9期50卷 1057-1069页

作者： Yin Li Zinan Wang Xiaofen Sun kexuan tang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Plant Biotechnology Research Center School of Agriculture and Biology Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Shanghai Jiao Tong University Shanghai 200030 China

As a member of an important group of lipid soluble antioxidants, tocopherols play a paramount role in the daily diet of humans and animals. Recently, genes required for tocochromanol biosynthesis pathway have been identified and cloned with the help of genomics-based approaches and molecular manipulation in the model organisms： Arabidopsis thaliana and Synechocystis sp. PCC 6803. At the basis of these foundations, genetic manipulation of tocochromanol biosynthesis pathway can give rise to strategies that enhance the level of tocochromanol content or convert the constitution of tocochromanol. In addition, genetic manipulations of the tocochromanol biosynthesis pathway provide help for the study of the function of tocopherol in plant systems. The present article summarizes recent advances and pays special attention to the functions of tocopherol in plants. The roles of tocopherol in the network of reactiv.e oxygen species, antioxidants and phytohormones to maintain redox homeostasis and the functions of tocopherol as a signal molecule in chloroplast-to- nucleus signaling to regulate carbohydrate metabolism are also discussed.

关键词： antioxidant carbohydrate metabolism genetic dissection programmed cell death redox homeostasis signal molecule tocopherol.

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Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

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Science Bulletin 2016年第1期61卷 18-25页

作者： Qian Shen Tingxiang Yan Xueqing Fu kexuan tang Key Laboratory of Urban Agriculture (South) Ministry of Agriculture Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University

Artemisinin, also known as qinghaosu, a sesquiterpene endoperoxide lactone isolated from the Chinese medicinal plant Artemisia annua L., is the most effective antimalarial drug which has saved millions of *** to its great antimalarial activity and low content in wild A. annua plants, researches focused on enhancing the artemisin yield in plants became a hotspot. Several families of transcription factors have been reported to participate in regulating the biosynthesis and accumulation of *** this review, we summarize recent investigations in these fields, with emphasis on newly identified transcription factors and their functions in artemisinin biosynthesis regulation, and provide new insight for further research.

关键词： Artemisia annua L. Artemisinin ,Transcription factor Biosynthesis

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Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance in Arabidopsis

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Journal of Integrative Plant Biology 2010年第4期52卷 400-409页

作者： zinan wang Ying Xiao Wansheng Chen kexuan tang Lei Zhang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai 200433 China Department of Pharmacy Changzheng Hospital second Military Medical University Shanghai 200003 China

Vitamin C （L-ascorbic acid, AsA） has important antioxidant and metabolic functions in both plants and animals. Once used, ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase （DHAR, EC 1.***.5.1）. To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress. A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana. Transgenic plants were biochemically characterized and tested for responses to oxidative stress. Western blot indicated that the dhar-transgene was successfully expressed. In homozygous T4 transgenic seedlings, DHAR overexpression was increased up to 1.5 to 5.4 fold, which enhanced foliar ascorbic acid levels 2- to 4.25-fold and ratio of AsA/DHA about 3- to 16-fold relative to wild type. In addition, the level of glutathione, the reductant used by DHAR, also increased as did its redox state. When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10μM paraquat, transgenic plants showed a larger AsA pool size, lower membrane damage, and a higher level of chlorophyll compared with controls. These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress.

关键词： Stress Tolerance Increased Vitamin Enhanced Recycling

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Diversity and versatile functions of metallothioneins produced by plants:A review

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Pedosphere 2020年第5期30卷 577-588页

作者： SAEED-UR-RAHMAN Muhammad KHALD Nan HUI Sadaf-Ilyas KAYANI kexuan tang Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)o f Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240(China) Key Laboratory of Urban Agriculture School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240(China)

Metal ions are essential for plant growth and development,but in excess,these compounds can become highly *** have adopted numerous ways to maintain metal homeostasis while mitigating adverse effects of excess metal ions,including phytochelatin and the metal-chelating proteins metallothioneins(MTs).A family of cysteine(Cys)-rich,intracellular,and low-molecular-weight(4-8 kDa)MTs are proteins found in nearly all phyla including plants,animals,and fungi,and they have the potential to scavenge reactive oxygen species and detoxify toxic metals including copper,cadmium,and *** on their Cys numbers and residues,MTs have been categorized into three major *** I MTs,which have highly conserved Cys residues,are found in animals,while class II MTs,with less conserved Cys residues,are present in plants and are classified further into four *** III MTs include phytochelatins,a group of enzymatically synthesized Cys-rich *** MTs have been an area of interest for five decades with extensive studies,which have been facilitated by advancements in instrumental techniques,protein science,and molecular biology ***,we reviewed current advances in our understanding of the regulation of MT biosynthesis,their expression,and their potential roles in the alleviation of abiotic stresses(i.e.,drought,salinity,and oxidative stresses)and heavy metal detoxification and homeostasis.

关键词： abiotic stress biosynthesis gene expression heavy metals reactive oxygen species enzyme regulation

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Progress in NMR-based metabolomics of Catharanthus roseus

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Frontiers of Agricultural Science and Engineering 2015年第3期2卷 195-204页

作者： Qifang PAN Jingya ZHAO Yuliang WANG kexuan tang Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Metabolomics has been rapidly developed as an important field in plant sciences and natural products *** the only natural source for a diversity of monoterpenoid indole alkaloids(MIAs),especially the low-abundance antitumor agents vinblastine and vincristine,Catharanthus roseus is highly valued and has been studied extensively as a model for medicinal plants *** to multistep enzymatic biosynthesis and complex regulation,genetic modification in the MIA pathway has resulted in complicated changes of both secondary and primary metabolism in ***,affecting not only the MIA pathway but also other *** at the metabolic level is necessary to increase knowledge on the genetic regulation of the whole metabolic network connected to MIA *** magnetic resonance(NMR)is a very suitable and powerful complementary technique for the identification and quantification of metabolites in the plant ***-based metabolomics has been used in studies of *** for pathway elucidation,understanding stress responses,classification among different cultivars,safety and quality controls of transgenic plants,cross talk between pathways,and diversion of carbon fluxes,with the aim of fully unravelling MIA biosynthesis,its regulation and the function of the alkaloids in the plant from a systems biology point of view.

关键词： Catharanthus roseus monoterpenoid indole alkaloids NMR metabolomics

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Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper

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Chinese Science Bulletin 2001年第20期46卷 1698-1703页

作者： SUN Xiaofen tang kexuan WAN Bingliang Ql Huaxiong LU Xinggui State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life SciencesFudan UniversityShanghai 200433China Hybrid Rice Research Center Hubei Academy of Agricultural SciencesWuhan 430064China

Mature seed-derived callo from two elite Chinese japonica rice (Oryza saliva L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromy-cin phosphotransferase gene (hpt), the reporter β-glu-curonidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns, we identified five independent homozygous lines containing and expressing all the three transgenes. Insect

关键词： Oryza saliva L. gna transgenic rice bioassay feeding test.

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Causal reasoning in typical computer vision tasks

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Science China(Technological Sciences) 2024年第1期67卷 105-120页

作者： ZHANG kexuan SUN QiYu ZHAO ChaoQiang tang Yang Key Laboratory of Advanced Control and Optimization for Chemical Process Ministry of EducationEast China University of Science and TechnologyShanghai 200237China National Key Laboratory of Air based Information Perception and Fusion Luoyang 471000China Luoyang Institute of Electro Optical Equipment of Avic Luoyang 471000China

Deep learning has revolutionized the field of artificial *** on the statistical correlations uncovered by deep learning-based methods,computer vision tasks,such as autonomous driving and robotics,are growing *** being the basis of deep learning,such correlation strongly depends on the distribution of the original data and is susceptible to uncontrolled *** the guidance of prior knowledge,statistical correlations alone cannot correctly reflect the essential causal relations and may even introduce spurious *** a result,researchers are now trying to enhance deep learningbased methods with causal *** theory can model the intrinsic causal structure unaffected by data bias and effectively avoids spurious *** paper aims to comprehensively review the existing causal methods in typical vision and visionlanguage tasks such as semantic segmentation,object detection,and image *** advantages of causality and the approaches for building causal paradigms will be *** roadmaps are also proposed,including facilitating the development of causal theory and its application in other complex scenarios and systems.

关键词： causal reasoning computer vision tasks vision-language tasks semantic segmentation object detection

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Molecular insights into AabZIP1-mediated regulation on artemisinin biosynthesis and drought tolerance in Artemisia annua

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1500-1513页

作者： Guoping Shu Yueli tang Mingyuan Yuan Ning Wei Fangyuan Zhang Chunxian Yang Xiaozhong Lan Min Chen kexuan tang Lien Xiang Zhihua Liao Key Laboratory of Eco-environments in Three Gorges Reservoir Region(Ministry of Education) Chongqing Engineering Research Centre for Sweet PotatoSWU-TAAHC Medicinal Plant Joint R&D CentreSchool of Life SciencesSouthwest UniversityChongqing 400715China TAAHC-SWU Medicinal Plant Joint R&D Centre Xizang Agricultural and Husbandry CollegeNyingchi 860000China College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China College of Environmental Science and Engineering China West Normal UniversityNanchong 637009China

Artemisia annua is the main natural source of artemisinin *** ***,extended drought stress severely reduces its biomass and artemisinin production while short-term water-withholding or abscisic acid(ABA)treatment can increase artemisinin ***-responsive transcription factor AabZIP1 and JA signaling AaMYC2 have been shown in separate studies to promote artemisinin production by targeting several artemisinin biosynthesis ***,we found AabZIP1 promote the expression of multiple artemisinin biosynthesis genes including AaDBR2 and AaALDH1,which AabZIP1 does not directly ***,it was found that AabZIP1 up-regulates AaMYC2expression through direct binding to its promoter,and that AaMYC2 binds to the promoter of AaALDH1to activate its *** addition,AabZIP1 directly transactivates wax biosynthesis genes AaCER1and *** biosynthesis of artemisinin and cuticular wax and the tolerance of drought stress were significantly increased by AabZIP1 overexpression,whereas they were significantly decreased in RNAi-AabZIP1 ***,we have uncovered the AabZIP1-AaMYC2 transcriptional module as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis,which may have general *** have also identified AabZIP1 as a promising candidate gene for the development of *** plants with high artemisinin content and drought tolerance in metabolic engineering breeding.

关键词： Artemisia annua AabZIP1 AaMYC2 Artemisinin biosynthesis Drought tolerance Wax biosynthesis

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