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湖南烟草镰刀菌根腐病病原鉴定及防治药剂筛选

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中国烟草科学 2024年第3期45卷 51-59,67页

作者：钟杰邱泽澜刘仕达周向平王迎黄焱斌周可心朱俊子刘天波肖艳松湖南农业大学植物保护学院长沙410128 湖南省烟草公司永州市公司湖南永州425000 湖南省烟草科学研究所长沙410010 湖南省烟草公司郴州市公司湖南郴州423000

为明确湖南郴州和永州地区烟草根腐病的病原种类,针对主要病原菌筛选有效的防治药剂,分别利用组织分离法和平板稀释法对两地主要烟区采集的烟草根腐病病样和根际土壤进行病原分离,通过形态学观察、翻译延伸因子1A(EF-1α)序列进化分析... 详细信息

为明确湖南郴州和永州地区烟草根腐病的病原种类,针对主要病原菌筛选有效的防治药剂,分别利用组织分离法和平板稀释法对两地主要烟区采集的烟草根腐病病样和根际土壤进行病原分离,通过形态学观察、翻译延伸因子1A(EF-1α)序列进化分析以及致病性测定确定病原种类,采用生长速率法测定供试杀菌剂对主要病原菌菌丝生长的抑制效果,并通过活体盆栽防效试验验证候选杀菌剂对主要病原菌的防治效果。共分离到真菌128株,其中镰刀菌87株,包括共享镰刀菌Fusarium commune、甜瓜镰刀菌Fusarium incarnatum、藤仓镰刀菌Fusarium fujikuroi、茄镰刀菌Fusarium solani、层出镰刀菌Fusarium proliferatum、Fusarium falciforme、Fusarium humuli共7个种。其中,***占比最高,为39.08%,其次为***和***,占比均为14.94%。致病力测定结果表明,这些菌株都能引起烟草发病,明确其为烟草根腐病的病原物。***对40%咪鲜胺EW、43 g/L戊唑醇SC和15%氰烯菌酯SC高度敏感,EC50分别为0.026、0.079和0.169μg/mL。盆栽试验结果表明,40%咪鲜胺EW的防治效果最为显著,相对防效达到94.43%±2.79%,其次为43 g/L戊唑醇SC和15%氰烯菌酯SC,相对防效为88.63%±1.68%和83.07%±2.55%。本研究表明,湖南郴州和永州地区烟草镰刀菌根腐病病原真菌以共享镰刀为主,供试药剂中,40%咪鲜胺EW对该病原菌的抑菌效果最好。

关键词：烟草根腐病镰刀菌致病性共享镰刀菌药剂筛选

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液液萃取-石墨炉原子吸收法测定食盐中铅含量的不确定度评定

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现代食品 2022年第8期28卷 175-179页

作者：邹勇平周元元杨嘉胡云周可欣梁志春王帅扬州市食品药品检验检测中心江苏扬州225100

本文建立了液液萃取-石墨炉原子吸收法测定食盐中铅含量的不确定度评定数学模型。针对液液萃取中存在的多次定容操作对结果带来的影响,分析和探讨了测定过程中不确定度来源,包括样品称量结果、待测溶液定容体积、工作曲线、测量结果的... 详细信息

本文建立了液液萃取-石墨炉原子吸收法测定食盐中铅含量的不确定度评定数学模型。针对液液萃取中存在的多次定容操作对结果带来的影响,分析和探讨了测定过程中不确定度来源,包括样品称量结果、待测溶液定容体积、工作曲线、测量结果的重复性和校准溶液浓度。将各分量进行合成,得到合成标准不确定度和扩展不确定度。当k=2(95%置信度水平),食盐中铅含量结果表示为(0.136±0.011)mg·kg^(-1)。结果不确定度的主要分量依次为工作曲线、测量结果的重复性和校准溶液浓度。在检验中,需要减少工作曲线定容次数和保证样品的均匀度以降低不确定度。

关键词：食盐铅不确定度液液萃取石墨炉原子吸收法

脑卒中患者作业治疗参与能力-机会-动机问卷的编制及信效度检验

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反射疗法与康复医学 2023年第5期4卷 159-162页

作者：许梦凡杨丽高子寒周可欣邝金珂山东省青岛大学护理学院山东青岛266000 山东省青岛市市立医院神经康复科山东青岛266000

目的编制脑卒中患者作业治疗参与能力-机会-动机问卷,并评价其信效度.方法以COM-B模型为理论框架,通过文献分析、专家函询形成初始版问卷.便利选取402例脑卒中患者进行问卷调查,进行项目分析和信效度检验.结果问卷包括3个维度、34个条目... 详细信息

目的编制脑卒中患者作业治疗参与能力-机会-动机问卷,并评价其信效度.方法以COM-B模型为理论框架,通过文献分析、专家函询形成初始版问卷.便利选取402例脑卒中患者进行问卷调查,进行项目分析和信效度检验.结果问卷包括3个维度、34个条目.探索性因子分析提取3个公因子,累计方差贡献率为84.309%.问卷的重测信度为0.878,Cronbach'sα系数为0.958,折半信度为0.961.结论编制的脑卒中患者作业治疗参与能力-机会-动机问卷具有良好的信效度,可评估脑卒中患者参与作业治疗的能力、机会和动机.

关键词：脑卒中问卷编制信效度

链霉素废水对斑马鱼POD活性和GSH含量的影响

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安全与环境工程 2015年第3期22卷 1-5,11页

作者：李敏赵月曹志会王冰周可心沈洪艳河北科技大学环境科学与工程学院河北石家庄050080 河北省药用分子化学实验室河北石家庄050080

为明确链霉素废水对斑马鱼的毒性效应,将斑马鱼暴露于10%、30%、50%和70%体积百分比的链霉素生产废水中,同时设置空白对照组,分别于第3、6、9、12和15天检测斑马鱼肌肉组织中过氧化物酶（POD）活性和还原型谷胱甘肽（GSH）含量两种生化... 详细信息

为明确链霉素废水对斑马鱼的毒性效应,将斑马鱼暴露于10%、30%、50%和70%体积百分比的链霉素生产废水中,同时设置空白对照组,分别于第3、6、9、12和15天检测斑马鱼肌肉组织中过氧化物酶（POD）活性和还原型谷胱甘肽（GSH）含量两种生化指标,考察链霉素废水对斑马鱼肌肉组织中POD活性和GSH含量的影响。结果表明：在低浓度组（10%体积百分比）废水中,斑马鱼肌肉组织中POD活性和GSH含量均呈现抑制—诱导—抑制的变化趋势;在中浓度组（30%和50%体积百分比）废水中,斑马鱼肌肉组织中POD活性基本维持在对照组水平,与对照组无显著性差异,而GSH活性变化反复,其中30%体积百分比试验组斑马鱼肌肉组织中POD活性和GSH含量表现出抑制—诱导—抑制—诱导的趋势,50%体积百分比试验组斑马鱼肌肉组织中POD活性和GSH含量表现出诱导—抑制—诱导的趋势;高浓度组（70%体积百分比）废水对斑马鱼的毒害作用比较明显,斑马鱼肌肉组织中POD活性和GSH含量同样表现出诱导—抑制的趋势。结果分析表明：斑马鱼暴露在10%~70%体积百分比的链霉素生产废水中15d,其肌肉组织中POD活性不如GSH含量敏感,在10%体积百分比链霉素生产废水中斑马鱼肌肉组织中POD活性和GSH含量变化呈现出抑制—诱导—抑制的变化趋势,在70%体积百分比链霉素生产废水中斑马鱼肌肉组织中POD活性和GSH含量变化呈现出诱导—抑制的趋势,由此说明斑马鱼已受到污染胁迫并产生应激反应,其中70%体积百分比的链霉素生产废水对斑马鱼的毒性作用最强,表明鱼体已受到较大氧化损伤。

关键词：链霉素废水斑马鱼 POD活性 GSH含量抗氧化防御体系

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Clinical Study on the Modified Formula of Huang Yuanyu’s Gui Fu Ling Wu Decoction for Treating Spleen-Kidney Yang Deficiency Type Diabetic Nephropathy

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Journal of Clinical and Nursing Research 2025年第1期9卷 32-38页

作者： Xiaoqin Liu Xiaoping zhou Wen Wang Meiji Li Qian Zhang Xiaoxia Zhai kexin Yu Changyi Hospital of Traditional Chinese Medicine Changyi 261300Shandong ProvinceChina

Objective:To evaluate the efficacy of the modified Gui Fu Ling Wu Decoction in treating diabetic nephropathy(DN)of the spleen-kidney Yang deficiency ***:A total of 100 DN patients admitted to Changyi Traditional Chinese Medicine Hospital between August 2023 and March 2024 were included in the *** were randomly divided into a control group and a study group,each comprising 50 cases,using a computerized random number *** control group received kallikrein treatment,while the study group received a combination of kallikrein and the modified Gui Fu Ling Wu *** glucose control,renal function,and inflammatory markers were assessed before and after treatment in both ***:Before treatment,there were no significant differences in blood glucose and renal function indicators between the two groups(P>0.05).After treatment,postprandial blood glucose,fasting blood glucose,24-hour urinary protein,urinary microalbumin,serum creatinine,serum C-reactive protein(CRP),and interleukin-6 levels significantly decreased in both groups,with the study group showing superior results compared to the control group(P<0.05).Conclusion:The combination of Gui Fu Ling Wu Decoction and kallikrein for treating spleen-kidney Yang deficiency type DN significantly improves blood glucose control,enhances renal function,and reduces inflammatory responses.

关键词： Gui Fu Ling Wu Decoction Diabetic nephropathy Renal function Inflammatory markers

Extraction and analysis of risk factors from Chinese chemical accident reports

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Chinese Journal of Chemical Engineering 2023年第9期61卷 68-81页

作者： Xi Luo Xiayuan Feng Xu Ji Yagu Dang Li zhou kexin Bi Yiyang Dai School of Chemical Engineering Sichuan UniversityChengdu 610065China

Accidents in chemical production usually result in fatal injury,economic loss and negative social *** accident reports which record past accident information,contain a large amount of expert ***,manually finding out the key factors causing accidents needs reading and analyzing of numerous accident reports,which is time-consuming and labor ***,in this paper,a semiautomatic method based on natural language process(NLP)technology is developed to construct a knowledge graph of chemical ***,we build a named entity recognition(NER)model using SoftLexicon(simplify the usage of lexicon)+BERT-Transformer-CRF(conditional random field)to automatically extract the accident information and risk *** risk factors leading to accident in chemical accident reports are divided into five categories:human,machine,material,management,and *** analysis of the extraction results of different chemical industries and different accident types,corresponding accident prevention suggestions are ***,based on the definition of classes and hierarchies of information in chemical accident reports,the seven-step method developed at Stanford University is used to construct the ontology-based chemical accident knowledge description ***,the ontology knowledge description model is imported into the graph database Neo4j,and the knowledge graph is constructed to realize the structu red storage of chemical accident *** the case of information extraction from 290 Chinese chemical accident reports,SoftLexicon+BERT-Transformer-CRF shows the best extraction performance among nine experimental *** that the method developed in the current work can be a promising tool in obtaining the factors causing accidents,which contributes to intelligent accident analysis and auxiliary accident prevention.

关键词： Chemical processes Chemical process safety Natural language process Knowledge graph Neural networks Algorithm

Potential Adverse Outcome Pathways of Chlorinated Organophosphate Flame Retardants

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China CDC weekly 2024年第23期6卷 542-546,I0008-I0011页

作者： Meiyu zhou Huilin Zhang Qi Xiao kexin Li Xiaoting Li Haiyan Chu Department of Environmental Genomics Jiangsu Key Laboratory of Cancer BiomarkersPrevention and TreatmentCollaborative Innovation Center for Cancer Personalized MedicineSchool of Public HealthNanjing Medical UniversityNanjing CityJiangsu ProvinceChina Department of Genetic Toxicology The Key Laboratory of Modern Toxicology of Ministry of EducationCenter of Global HealthSchool of Public HealthNanjing Medical UniversityNanjing CityJiangsu ProvinceChina Department of Nutrition and Food Safety School of Public HealthNanjing Medical UniversityNanjing CityJiangsu ProvinceChina

What is already known about this topic?Chlorinated organophosphate flame retardants(Cl-OPFRs)are frequently detected chemicals in the environment and biological samples,yet there is a lack of systematic evaluation regarding the adverse effects and toxicological mechanisms of *** is added by this report?This study utilizes the adverse outcome pathway(AOP)framework to assess the health implications and mechanisms of Cl-OPFRs,identifying multi-system toxicity,with a particular emphasis on reproductive issues and the possible toxic *** are the implications for public health practice?These results enhance knowledge of the health hazards linked to Cl-OPFRs,supporting the creation of focused risk evaluations and suitable regulatory actions.

关键词： utilize supporting Chlorin

Core bacterial communities dominated Purus frumentum biomass under different green manure returning amounts in saline-alkali soil

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Soil Ecology Letters 2024年第4期6卷 149-162页

作者： Zhibo zhou Yongyong Ding kexin Li Fengge Zhang College of Agro-grassland Science Nanjing Agricultural UniversityNanjing 210095China College of Life and Environmental Sciences Hunan University of Arts and ScienceChangde 415000China

*** biomass could be improved by appropriating returning *** biomass was excellent in 75%alfalfa returning *** species of bacteria differed among the alfalfa returning amountsThe relationship of core bacteria and their potential ecological functions are more close to *** use of green manure returning to field is a common practice in conservation ***,there is limited research on how different amounts of alfalfa can affect saline-alkali soil properties,bacterial community characteristics,and subsequent *** this study,five different amounts of alfalfa return were investigated to understand the biological relationships between rhizospheres soil properties,bacterial communities,potential functions,and the Purus frumentum *** results showed that the biomass was highest when 75%of the alfalfa was returned to the *** particular amount was associated with relatively low soil pH and electrical ***,it increased the relative abundance of beneficial bacterial taxa in both core and non-core *** analysis revealed significant differences in both core(RANOSIM=0.871,P=0.001)and non-core(RANOSIM=0.947,P=0.001)bacterial communities among the different amounts of alfalfa return based on non-metric multidimensional scaling *** bacterial taxa and their potential ecological functions were more closely related to plant biomass compared to non-core bacteria based on correlation analysis and multiple regression ***,our results indicate that optimizing the amount of alfalfa return can improve subsequent plant *** soil physicochemical properties and influencing core microbial community structure are of great significance for soil functional stability and crop productivity sustainability.

关键词： alfalfa green manure core bacteria FAPROTAX saline-alkali soil

Regional Δ~14C patterns and fossil fuel derived CO_2 distribution in the Beijing area using annual plants

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Chinese Science Bulletin 2011年第16期56卷 1721-1726页

作者： XI XianTing DING XingFang FU DongPo zhou LiPing LIU kexin Institute of Heavy Ion Physics & State Key Laboratory of Nuclear Physics and Technology Peking University Beijing 100871 China College of Urban and Environmental Science & Laboratory for Earth Surface Process Peking University Beijing 100871 China

The level of atmosphericΔ14C and the fossil fuel derived CO 2 concentration in the Beijing area from May to September,2009, were systematically analyzed based on radiocarbon(14C)measurements of annual plants by accelerator mass spectrometry(AMS). The results show that the maximumΔ14C in Beijing was 29.6‰±2.2‰,and the minimum was–28.2‰±2.5‰,with a trend of decreasingΔ14C from the outer suburbs to inner suburbs to the urban *** trend correlates well with increases in fossil fuel derived CO2 caused by human activities such as population density,industrial emissions and traffic,with lower values of atmosphericΔ14C associated with more intensive human *** fossil fuel derived CO 2 concentrations from May to September, 2009,ranged from 3.9±1.0 ppm to 25.4±1.0 *** was calculated that each additional 1 ppm of CO2 from fossil fuels depleted the atmosphericΔ14C by approximately 2.70‰.This study suggests that 14C measurements of annual plants by AMS provide an effective method to rapidly trace fossil fuel derived CO2.

关键词：二氧化碳浓度一年生植物化石燃料北京地区衍生碳分布 C模式加速器质谱