检索结果-南通市图书馆

Neural Regeneration Research 2012年第22期7卷 1709-1714页

作者： Yan wang Ning Zhu kewan wang Zhongyi Zhang Yong wang Department of Pharmacy Zhujiang Hospital Southern Medical University Guangzhou 510282 Guangdong Province China Department of Pharmacy the 458 Hospital of Chinese PLA Guangzhou 510602 Guangdong Province China Department of Neurosurgery Nanfang Hospital Southern Medical University Guangzhou 510602 Guangdong Province China

The present study found expressions of a7 nicotinic acetylcholine receptor on hippocampal slices and hippocampal astrocytes using double immunofluorescence stainings. Expression of glial fibdllary acidic protein in the cultured hippocampal slices and hippocampal astrocytes significantly increased, and levels of macrophage inflammatory protein la, RANTES, interleukin-1β, intedeukin-6, and tumor necrosis factor-α increased in the supernatant of cultured astrocytes following exposure to 200 nM amyloid 13 protein 1-42. Preconditioning of 10 μM nicotine, a nicotinic acetylcholine receptor agonist, could attenuate the influence of amyloid β protein 1-42 in inflammatory mediator secretion of cultured astrocytes. Experimental findings indicated that α7 nicotinic acetylcholine receptor was expressed on the surface of hippocampal astrocytes, and activated a7 nicotinic acetylcholine receptor was shown to inhibit inflammation induced by amyloid β protein 1-42.

关键词： α7 nicotinic acetylcholine receptor astrocytes inflammation cytokines chemotactic factor amyloidβ protein hippocampus neural regeneration

Huperzine A protects neural stem cells against Aβ induced apoptosis in a neural stem cells and microglia co-culture system

在线全文

学校读者我要写书评

暂无评论

Huperzine A protects neural stem cells against Aβ induced a...

Timing of globuspallidu internal deep brain stimulation for cervical dystonia:case report and systemic review

2016年广东省药师周大会

作者： Ning Zhu Jizong Lin kewan wang Meidan Wei Qingzhuang Chen Yong wang Department of Pharmacy Zhujiang HospitalSouthern Medical University Department of Hepatobiliary Surgery the Third Affiliated HospitalSun Yat-Sen University Department of Neurosurgery Nanfang HospitalSouthern Medical University

Objectives This study aims to explore whether Huperzine A(Hup A) could protect neural stem cells against Amyloid beta-peptide Aβ induced apoptosis in a neural stem cells(NSCs) and microglia co-culture system Methods Rat NSCs and microglial cells were isolated,cultured and identified with immunofluorescence Assays(IFA).Co-culture systems of NSCs and microglial cells were employed using Transwell Permeable *** effects of Aβ1-42 on NSCs were studied in 4 groups using co-culture systems:NSCs,Aβ+NSCs,co-culture and Aβ+co-culture ***(Brd U) incorporation and flow cytometry were utilized to assess the differences of proliferation,differentiation and apoptosis of NSCs between the *** test was performed to assess the amounts of IL-6,TNF-α and MIP-α secreted,and flow cytometry and Western blotting were used to assess apoptosis of NSCs and the expressions of Bcl-2 and Bax in each *** IFA results showed that isolated rat NSCs were nestin-positive and microglial cells were CD11 b/*** all the groups,the Aβ+co-culture group has the lowest Brd U expression level,the lowest MAP2-positive,Ch AT-positive cell counts and the highest NSC apoptosis *** amounts of IL-6,TNF-α and MIP-α were being secreted by microglial cells in the Hup A+Aβ+co-culture group compared with those in the Aβ+co-culture *** the Bcl-2:Bax ratio was much higher in the Hup A+Aβ+co-culture group than in the Aβ+co-culture *** Hup A inhibits cell apoptosis through restraining microglia’s inflammatory response induced by Aβ.

关键词： Huperzine A Alzheimer’s disease Amyloid beta-peptide neural stem cells microglial cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Timing of globuspallidu internal deep brain stimulation for ...

Blocking mPTP on neural stem cells and activating the nicotinic acetylcholine receptor α7 subunit on microglia attenuate Aβ-induced neurotoxicity on neural stem cells

第十五届中国医师协会神经外科医师年会

作者： Jun wang Zhang Yuzhen Hao Long Kaijun Yang kewan wang Hongxiao wang Songtao Qi Nanfang Hospital Southern Medical University The First Clinical Medicine College Southern Medical University Neural Networks Surgery Team Southern Medical University

＜正＞目的:Cervical dystonia is the most common form of focal dystonia,and it often cause pain and *** surgical therapies,deep brain stimulation（DBS） of the internal globuspallidus（GPi） has showed its safety and efficacy and has emerged as the treatment of choice for adults with cervical dystonia who do not respond to pharmacologic *** there much controversy over the timing of surgical intervention in cervical dystonia.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Blocking mPTP on neural stem cells and activating the nicoti...

试论作为概念的“宗教”——从威尔弗雷德·坎特韦尔·史密斯的《宗教的意义与终结》谈起

2017年广东省药师周大会

作者： Qingzhuang Chen kewan wang Deqi Jiang Yan wang Xiaodan Xiao Ning Zhu Mingxing Li Siyuan Jia Yong wang Department of Pharmacy Southern Medical UniversityZhujiang Hosipital Department of Clinical Pharmacy Guangzhou Hospital of Integrated Traditional and Western Medicine Department of Neurosurgery Nanfang HospitalSouthern Medical University

Background: β-Amyloid(Aβ) can stimulate microglia to release a variety of proinflammatory cytokines and induce neurotoxicity. Nicotine has been reported to inhibit TNF-α, IL-1, and ROS production in microglia. Mitochondrial permeability transition pore(mP TP) plays an important role in neurotoxicity as well. Here, we investigated whether activating the microglial α7-nAChR has a neuroprotective role on neural stem cells(NSCs) and the function of mP TP in NSCs in this process. Methods: The expression of α7-nAChR in rat NSCs was detected by immunocytochemistry and RT-PCR. The viability of microglia and NSCs was examined by MTT assay. The mitochondrial membrane potential(ΔΨm) and morphological characteristics of NSCs was measured by JC-1 staining and transmission electron microscopy respectively. The distribution of cytochrome c in the subcellular regions of NSCs was visualized by confocal laser scanning microscopy, and the expression levels of cyclophilin D and cleaved caspase-3 were assayed by western blot. The apoptotic rate of NSCs was measured by flow cytometry. Results: The expression of α7-nAChR was detected in microglial cells, but no expression was found in NSCs. The viability of rat microglial cells and NSCs was not affected by reagents or coculture itself. Aβ1-42-mediated microglial activation impaired the morphology and the ΔΨm of mitochondria of NSCs as well as increased cell apoptosis. However, the damage was attenuated when the α7-nAChRs on microglial cells were activated or the m PTPs on NSCs were blocked. Conclusions: Blockade of mP TPs on NSCs and activation of α7-nA ChR s on microglia exhibit neuroprotective roles in Aβ-induced neurotoxicity of NSCs.

关键词： mitochondrial permeability transition pore microglia neural stem cells inflammatory response β-amyloid nicotinic acetylcholine receptor α7 subunit

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

广州社会主义学院学报 2010年第3期8卷 69-72页

作者：王克琬中央民族大学哲学与宗教学学院

威尔弗雷德.坎特韦尔.史密斯通过对"宗教"概念的演化历史的考察和分析,得出的结论是我们应该放弃这个概念而以"信仰"和"累积的传统"这两个单独的概念来取代它。本文认为这种观念虽有见地,但这种术语的变革未必能够达成,更为现实的做法... 详细信息

威尔弗雷德.坎特韦尔.史密斯通过对"宗教"概念的演化历史的考察和分析,得出的结论是我们应该放弃这个概念而以"信仰"和"累积的传统"这两个单独的概念来取代它。本文认为这种观念虽有见地,但这种术语的变革未必能够达成,更为现实的做法是我们在使用这个概念时对语言的歧义性和误导性抱以警觉,改变思维方式,放弃对宗教本质的追求而把它视为一个集合名称而在特定的研究范围或语境中为它暂定界限。

关键词：宗教诸宗教本质集合名称

在线全文

学校读者我要写书评

暂无评论

罪就是人的异化——蒂里希罪论思想初探

广州社会主义学院学报 2010年第1期8卷 53-57页

作者：王克琬王再兴中央民族大学哲学与宗教学学院襄樊学院

在基督教神学领域中,罪论是一个相当复杂而又颇富争议性的问题。蒂里希的罪论思想建立在哲学本体论思辨的基础之上,同时又交织着深刻的人文主义关怀。他主张,罪的本质特征及其产生根源两者都可以归结为人的生存异化。本文拟对蒂里希罪... 详细信息

在基督教神学领域中,罪论是一个相当复杂而又颇富争议性的问题。蒂里希的罪论思想建立在哲学本体论思辨的基础之上,同时又交织着深刻的人文主义关怀。他主张,罪的本质特征及其产生根源两者都可以归结为人的生存异化。本文拟对蒂里希罪论思想的这一方面进行探讨并作出自己的评价。

关键词：罪异化本质现实

在线全文

学校读者我要写书评

暂无评论

多功能小型生物撞击机的研制与应用

生物医学工程学杂志 2000年第3期17卷 309-312,323页

作者：尹志勇王正国刘海鹏谢柏林王克万董蕻第三军医大学大坪医院野战外科研究所重庆400042

研制一套集柔性撞击、刚性撞击和细胞损伤于一体的多功能致伤装置及简易伤情评估系统。应用空气动力学原理、微弱信号检测技术和机电一体化的方法研制而成 ,通过更换撞击头可实现柔性撞击、刚性撞击和细胞损伤 ,通过眼、脑、肺和细胞进... 详细信息

研制一套集柔性撞击、刚性撞击和细胞损伤于一体的多功能致伤装置及简易伤情评估系统。应用空气动力学原理、微弱信号检测技术和机电一体化的方法研制而成 ,通过更换撞击头可实现柔性撞击、刚性撞击和细胞损伤 ,通过眼、脑、肺和细胞进行实验研究。该系统通过微机对致伤参数进行精确控制 ,实时测试致伤参数 ,并对损伤前后的神经功能进行评估。实验结果表明 :采用不同的驱动压可分别造成轻、中、重度的损伤模型 :柔性撞击致大鼠脑损伤为 40 0、6 0 0、70 0 k Pa;兔视网膜挫伤为 6 0 0、80 0、10 0 0 k Pa;刚性撞击致大鼠肺损伤为 30 0、45 0、6 0 0 k Pa;细胞损伤为 10 0、15 0、2 0 0 k Pa。本装置具有操作简便、定量可控、重复性好、应用广泛等优点 ,可非常方便地复制出小动物不同部位 ,不同种类和不同程度的损伤模型。

关键词：生物撞击机动物模型细胞损伤

内侧隔-斜角带核:阿片类药物精神依赖外科治疗的新靶点(附34例报告)

在线全文

学校读者我要写书评

暂无评论

中国微侵袭神经外科杂志 2006年第3期11卷 112-115页

作者：王克万漆松涛杨开军南方医科大学附属南方医院神经外科广东广州510515

目的探讨单纯双侧内侧隔-斜角带核毁损对阿片类药物精神依赖的治疗作用。方法采用立体定向毁损双侧内侧隔-斜角带核后部治疗34例阿片类药物精神依赖病人,观察手术前后成瘾个体对药物的精神依赖和尼古丁依赖的变化情况,采用简明心理状况... 详细信息

目的探讨单纯双侧内侧隔-斜角带核毁损对阿片类药物精神依赖的治疗作用。方法采用立体定向毁损双侧内侧隔-斜角带核后部治疗34例阿片类药物精神依赖病人,观察手术前后成瘾个体对药物的精神依赖和尼古丁依赖的变化情况,采用简明心理状况检查量表(MMSE)、汉密尔顿抑郁量表(HAMD)和焦虑量表(HAMA)评价手术对记忆、情感的影响。结果随访12￣18个月,精神依赖完全消除25例(73.5%),复吸9例。术后病人学习和记忆力无明显变化,抑郁和焦虑症状有显著性改善(P<0.01)。结论单纯内侧隔-斜角带核毁损治疗阿片类药物依赖有效。

关键词：隔核 Broca斜角带阿片相关性障碍立体定位技术

立体定向放射外科治疗功能区顽固性癫的研究

在线全文

学校读者我要写书评

暂无评论

中国微侵袭神经外科杂志 2011年第9期16卷 398-400页

作者：徐波涛杨开军王克万王洪筱李玉芬漆松涛南方医科大学南方医院神经外科广州510515

目的评价立体定向放射外科在功能区顽固性癫疒间的治疗作用。方法回顾性分析功能区顽固性癫疒间85例。根据癫疒间发作特点、脑电图及影像学检查综合评估和定位。采用Brainscan X-刀治疗系统实施治疗,中心剂量12~16 Gy,周边剂量9~13 Gy... 详细信息

目的评价立体定向放射外科在功能区顽固性癫疒间的治疗作用。方法回顾性分析功能区顽固性癫疒间85例。根据癫疒间发作特点、脑电图及影像学检查综合评估和定位。采用Brainscan X-刀治疗系统实施治疗,中心剂量12~16 Gy,周边剂量9~13 Gy。随访3~118个月,平均70个月。结果脑电图改变在术后12个月达到高峰,28.6%恢复正常,46.8%明显好转;发作频率在术后6个月明显降低,24个月降低达治疗谷峰;疗效在术后24个月达到高峰,有效率89.1%。术后影像学检查显示放射性脑水肿59例,其中出现症状需处理6例,经治疗后均恢复;短期内一过性癫疒间发作频率升高8例,无致残和死亡病例。结论立体定向放射外科治疗功能区顽固性癫疒间效果良好,可作为无法手术病人的替代治疗方法。

关键词：癫疒间,顽固性脑电描记术放射外科手术功能区

在线全文

学校读者我要写书评

暂无评论

二醋酯纤维与棉混纺针织物染整工艺探讨

天津纺织科技 2024年第3期 55-59页

作者：解晓然李克弯刘浩戈剑广东传化富联精细化工有限公司广东佛山528329 伊士曼(中国)投资管理有限公司上海201210 杭州福莱蒽特股份有限公司浙江杭州311228

二醋酯纤维诞生已有近百年的历史,但因棉与二醋酯纤维混纺面料在染整过程仍然存在一些技术难点,目前二醋酯纤维与棉的混纺应用相对较少。文中主要探讨二醋酯纤维与棉混纺织物在染整过程中的技术难点,主要针对二醋酯纤维遇碱皂化以及色... 详细信息

二醋酯纤维诞生已有近百年的历史,但因棉与二醋酯纤维混纺面料在染整过程仍然存在一些技术难点,目前二醋酯纤维与棉的混纺应用相对较少。文中主要探讨二醋酯纤维与棉混纺织物在染整过程中的技术难点,主要针对二醋酯纤维遇碱皂化以及色牢度偏低的技术难点,提供详细的低温前处理方案、酸性还原清洗方案、低温皂洗方案以及高效涤棉固色等解决方案,为二醋酯纤维与棉混纺织物的研究以及工业化生产提供参考。

关键词：二醋酯纤维混纺织物前处理抗皱低温皂洗涤棉固色