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Time Dependent Motility of Stallion Semen during Cool Storage Centrifuged in Two Different Extenders

Journal of Agricultural Science and Technology(A) 2022年第1期12卷 15-21页

作者： Christine Stroink-Pickering Yuna Lee Peyton Arnett kevin halden kline Department of Animal Sciences University of Illinois at Urbana Champaign1207 West Gregory DriveUrbanaIL 61801USA

The aim of this study was to evaluate the maintenance of spermatozoa motility over 72 h by two commercial semen extenders used to centrifuge semen before re-suspending the sperm pellet in INRA 96^(■).Centrifuged and re-suspended semen samples were evaluated for percent motility using four commercial Standard bred stallions collected three times each during the spring and summer breeding *** Generation ***’s^(■)without antibiotic or INRA 96^(■)semen extenders were used to dilute semen samples in a 1:1 ratio prior to centrifugation at 750×g for 10 *** decanting the supernatant,the resulting sperm pellets were then re-suspended and extended with INRA 96^(■)to a final concentration of 50 million total cells per millimeter and stored in Equine Express II stallion semen shipping *** progressive motility was recorded at time points 0,24,48,and 72 *** of variance between stallions,time,and extender type found that there was no significant difference in percent motility at each of the time points between the two extenders(p=0.87)used for *** in percent motility over time and between stallions were found(p<0.001).The findings suggest that the use of Next Generation ***’s^(■)semen extender during centrifugation and then re-suspending the sperm pellet in INRA 96^(■)may be more cost effective than using INRA 96^(■)for both centrifugation and re-suspension of the sperm *** is due to the fact that the less expensive Next Generation ***’s^(■)supernatant is decanted and discarded after the centrifugation step,instead of the much more expensive INRA 96^(■),yet does not affect the quality of semen preservation and motility over time.

关键词： Storage breeding millimeter

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Motility of Stallion Spermatozoa after Centrifugation and Cooling in INRA96 or Walworth Extender

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Journal of Agricultural Science and Technology(A) 2016年第2期6卷 143-147页

作者： Gabrielle Nicolette Riccio Robyn Ellienne Ellerbrock Igor Frederico Canisso Robert Victor Knox kevin halden kline Department of Animal Sciences University of Illinois Urbana-Champaign 1207 West Gregory Drive Urbana IL 61801 USA Department of Veterinary Clinical Medicine College of Veterinary Medicine University of Illinois Urbana-Champaign Urbana IL 61801 USA

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Feeder Type Affects Growth in Weanling Horses

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Journal of Agricultural Science and Technology(A) 2015年第7期5卷 616-620页

作者： Carol Jean Thompson kevin halden kline Department of Animal Sciences University of Illinois Urbana IL 61801 USA

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Effects of Reduced Glutathione to Centrifuged and Non-centrifuged Fresh Chilled Stallion Semen on Progressive Motility of Spermatozoa over 72 h

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Journal of Agricultural Science and Technology(A) 2020年第5期10卷 257-264页

作者： James Robert Lyko Leigha Ann Maree Johnson kevin halden kline Department of Animal Sciences University of Illinois at Urbana Champaign1207 West Gregory DriveUrbanaIL 61801USA

The aim of the current study was to evaluate the effects of supplementation of an inexpensive stallion semen extender(Next Generation■ ***’s with Amikacin and K-Penn)with 1 mM of the antioxidant reduced glutathione(GSH)on the progressive motility of cooled stallion semen over a period of 72 *** centrifuged and non-centrifuged semen samples were evaluated from six Standardbred stallions collected three times each,with one week between collections for each *** centrifuged samples,non-cushioned centrifugation was done at 750 g for 10 *** semen samples were extended to a final concentration of 50 million total cells per milliliter and stored in Equine Express II stallion semen shipping ***-centrifuged samples without GSH declined from an initial mean progressive motility of 70.3%at 0 h to 9.1%at 72 ***-centrifuged samples with added GSH declined from an initial mean progressive motility of 70.3%at 0 h to 7.1%at 72 *** samples without added GSH declined from an initial mean progressive motility of 70.3%at 0 h to 46.1%at 72 *** samples with added GSH declined from an initial mean progressive motility of 70.3%at 0 h to 40.3%at 72 *** results of this study did not find significant improvement in the progressive motility over time for spermatozoa prepared in a traditional skim-milk based extender with antibiotics as a result of addition of GSH to either centrifuged or non-centrifuged *** findings suggest that centrifugation of fresh chilled extended stallion semen may be a good idea for any semen that may be used at time points greater than 24 h after collection.

关键词： Antioxidant reduced glutathione Standardbred stallion cooling spermatozoa

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