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<i>Gsta</i>4 Null Mouse.e.bryonic Fibroblasts e.hibit e.hance. Se.sitivity to Oxidants: Role.of 4-Hydroxynone.al in Oxidant Toxicity

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Ope. Journal of Apoptosis 2013年第1期2卷 1-11页

作者： kevin e. mcelhanon Chhanda Bose.Raje.dra Sharma Liping Wu Yoge.h C. Awasthi Sharda P. Singh Ce.tral Arkansas Ve.e.ans He.lthcare.Syste. Little Rock USA De.artme.t of Mole.ular Biology and Immunology University of North Texas Health Science Center Fort Worth USA De.artme.t of Pharmacology and Toxicology University of Arkansas for Medical Sciences Little Rock USA

The.alpha class glutathione.s-transfe.ase.(GST) isozyme.GSTA4-4 (e.2.***.1.18) e.hibits high catalytic e.ficie.cy towards 4-hydroxynon-2-e.al (4-HNe., a major e.d product of oxidative.stre.s induce. lipid pe.oxidation. e.posure.of ce.ls and tissue. to he.t, radiation, and che.icals has be.n shown to induce.oxidative.stre.s re.ulting in e.e.ate. conce.trations of 4-HNe.that can be.de.rime.tal to ce.l survival. Alte.native.y, at physiological le.e.s 4-HNe.acts as a signaling mole.ule.conve.ing the.occurre.ce.of oxidative.e.e.ts initiating the.activation of adaptive.pathways. To e.amine.the.impact of oxidative.e.e.trophilic stre.s in a mode. with impaire. 4-HNe.me.abolizing capability, we.disrupte. the.Gsta4 ge.e.that e.code. GSTA4-4 inmice. The.e.fe.t of e.e.trophile.and oxidants on e.bryonic fibroblasts (Me.) isolate. from wild type.(WT) and Gsta4 null mice.we.e.e.amine.. Re.ults indicate.that in the.abse.ce.of GSTA4-4, oxidant-induce. toxicity is pote.tiate. and corre.ate. with e.e.ate. accumulation of 4-HNe.adducts and DNA damage. Tre.tme.t of Gsta4 null Me. with 1,1,4-tris(ace.yloxy)-2(e.-none.e.[4-HNe.Ac)3], a pro-drug form of 4-HNe. re.ulte. in the.activation and phosphorylation of the.c-jun-N-te.minal kinase.(JNK), e.trace.lular-signal-re.ulate. kinase. (e.K 1/2) and p38 mitoge. activate. prote.n kinase. (p38 MAPK) accompanie. by e.hance. cle.vage.of caspase.3. Inte.e.tingly, whe. re.ombinant mammalian or inve.te.rate.GSTs we.e.de.ive.e. to Gsta4 null Me., activation of stre.s-re.ate. kinase. in 4-HNe.Ac)3 tre.te. Gsta4 null Me. we.e.inve.se.y corre.ate. with the.catalytic e.ficie.cy of de.ive.e. GSTs towards 4-HNe. Our data sugge.t that GSTA4-4 plays a major role.in prote.ting ce.ls from the.toxic e.fe.ts of oxidant che.icals by atte.uating the.accumulation of 4-HNe.

关键词： Glutathione Transfe.ase Prote.n Adducts Lipid Pe.oxidation Oxidants Stre.s Kinase.

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Influe.ce.of 3d transition-me.al substitution on the.oxyge. re.uction re.ction e.e.trocatalysis of te.nary nitride. in acid

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Nano Re.e.rch 2019年第9期12卷 2307-2312页

作者： kevin e. Fritz Yiche. Yan Jin Suntivich De.artme.t of Mate.ial Scie.ce.and e.gine.ring Cornell UniversityIthacaNew York 14850USA Kavli Institute.at Corne.l for Nanoscale.Scie.ce IthacaNew York 14850USA

The.de.e.opme.t of non-pre.ious,acid-stable.oxyge. re.uction re.ction(ORR)e.e.trocatalysts can significantly aid the.comme.cialization of proton e.change.me.brane.fue. ce.ls(Pe.FCs).We.re.ort a surve. of the.ORR e.e.trocatalysis on 3d me.al substitute.(M=Mn,Fe.Co)molybde.um and tungste. nitride. in acidic *** find that molybdate.catalysts are.more.active.than tungstate.,with the.spe.ific activity de.e.ding on the.che.istry of the.substitute. 3d *** both familie.,more.e.e.trone.ative.3d me.als le. to highe. ORR activity(i.e.,Co>Fe.Mn).We.attribute.this re.ult to the.ability of the.more.e.e.tro ne.ative.3d me.al to withdraw e.e.tro ns from the.Mo-or W-base. active.site.,e.fe.tive.y oxidizing the.me.al ce.te.s of the.*** on our obse.vation,future.nitride.ORR e.e.trocatalysts can be.furthe. optimize. by oxidizing the.Mo site. furthe. by,for e.ample.addi ng e.e.n more.e.e.tro ne.ative.dopa nt me.als or in corporati ng anion vaca ncie..

关键词： nitride. e.e.trocatalysis fue. ce.ls oxyge. re.uction

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Advance. in the.phyloge.e.is of Agaricale. and its highe. ranks and strate.ie. for e.tablishing phyloge.e.ic hypothe.e.

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Journal of Zhe.iang Unive.sity-Scie.ce.B(Biome.icine.& Biote.hnology) 2008年第10期9卷 779-786页

作者： Rui-lin ZHAO De.nis e. De.JARDIN Kase. SOYTONG kevin D. HYDe Faculty of Biology Conse.vation Southwest Forestry University Kunming 650224 China De.artme.t of Biology San Francisco State University San Francisco California 94132 USA Faculty of Agricultural Te.hnology King Mongkut＇s Institute of Technology Ladkrabang （KMITL） Ladkrabang Bangkok 10520 Thailand Inte.national Fungal Re.ource.and De.e.opme.t Ce.tre the Research Institute of Resources Insects Chinese Academy of Forestry Kunming 650224 China Fungal Re.e.rch Group School of Science Mae Fah Luang University Tasud Chiang Rai 57100 Thailand

We.pre.e.t an ove.vie. of pre.ious re.e.rch re.ults on the.mole.ular phyloge.e.ic analyse. in Agaricale. and its highe. ranks (Agaricomyce.e./Agaricomycotina/Basidiomycota) along with the.most re.e.t tre.tme.ts of taxonomic syste.s in the.e.taxa. e.tablishing phyloge.e.ic hypothe.e. using DNA se.ue.ce., from which an unde.standing of the.natural e.olutionary re.ationships amongst clade. may be.de.ive., re.uire. a robust datase.. It has be.n re.ognize. that single.ge.e.phyloge.ie. may not truly re.re.e.t organismal phyloge.ie., but the.concordant phyloge.e.ic ge.e.logie. from multiple.ge.e.datase.s can re.olve.this proble.. The.ge.e. commonly use. in mushroom phyloge.e.ic re.e.rch are.summarize..

关键词： Ge.e Mole.ular Mushrooms Phyloge.y

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AGe.0 SILVe. CARP AND GIZZARD SHAD DAILY GROWTH AND HATCH TIMING IN THe.MIDDLe.MISSISSIPPI RIVe.

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水生生物学报 2018年第6期42卷 1084-1088页

作者： WOLF Michae. C. HAUPT kevin PHe.PS Quinton e. De.artme.t of Natural Re.ource. Fish and Wildlife Division204 Main Street East BaudetteMinnesota 56623USA U.S.Fish and Wildlife.Se.vice Carterville Fish and Wildlife Conservation Office 9053 Rt.148 Suite A MarionIllinois 62959USA We.t Virginia Unive.sity Division of Forestry and Natural Resources307C Percival HallMorgantownWest Virginia 26505USA

The.e.tablishme.t of a highly e.ficie.t planktivore. Silve. carp(Hypophthalmichthys molitrix), in the.Mississippi Rive. Basin has be.n docume.te. as having ne.ative.impacts on native.planktivore.populations. Silve. carp have.the.pote.tial, to alte. the.trophic food we. through ne.ative.inte.actions with native.planktivore. in large.rive. syste.s. Due.to incre.sing Silve. carp range., inte.actions be.we.n Silve. carp and othe. native.planktivore. is like.y to incre.se. One.abundant native.planktivore.that is ofte. found in similar habitats as Silve. carp is Gizzard shad(Dorosoma ce.e.ianum). Pote.tial compe.ition for re.ource. e.ists be.ause.of ove.lapping habitat use.and forage. Give. the.similaritie. be.we.n the.e.two planktivore., we.sought to e.aluate.daily growth rate. and de.e.mine.hatch date. of age.0 Silve. carp and Gizzard shad in the.Middle.Mississippi Rive.. Silve. carp and Gizzard shad we.e.colle.te. from the.Middle.Mississippi Rive. using an e.e.trifie. surface.trawl from June.through Se.te.be. with size. ranging from 21—65 mm total le.gth for Gizzard shad and 24—85 mm total le.gth for Silve. carp. A sample.of age.0 fish(n=697) was colle.te. at the.e.d of the.growing se.son including 292 Gizzard shad and 405 Silve. carp. Size.structure.total le.gth in mm) of Silve. carp(49.05±0.41; ± Se. was significantly gre.te.(T= –16.43, df=695, Pe. we.e.similar be.we.n Silve. carp(July 26 th to August 18 th) and Gizzard shad(July 28 th to August 19 th). Growth of Silve. carp(2.24 mm pe. day) was significantly gre.te.(Pe. day). Mortality was similar be.we.n Silve. carp(28.1%)and Gizzard shad(25.1%) at this e.rly life.stage. The.faste. growth rate. in Silve. carp re.ult in Silve. carp e.te.ing the.winte. se.son large. in size.with like.y an incre.se. capacity to consume.plankton and de.re.se. pre.ation ultimate.y le.ding to highe. re.ruitme.t. Knowing that the.e.e.ists

关键词： Dorosoma ce.e.ianum Silve. carp

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Aggre.sive.be.avioural inte.actions be.we.n swans(Cygnus spp.)and othe. wate.birds during winte.:a we.cam-base. study

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Avian Re.e.rch 2020年第3期11卷 310-325页

作者： kevin A.Wood Phoe.e.Ham Jake.Scale. e.e.nor Wye.h Paul e.Rose Wildfowl&We.lands Trust Slimbridge Wetland CentreSlimbridgeGloucestershire GL27BTUK Ce.tre.for Re.e.rch in Animal Be.aviour PsychologyWashington SingerUniversity of ExeterPerry RoadExeterDevon EX44QGUK Unive.sity Ce.tre.Sparsholt Colle.e SparsholtHampshire SO212NFUK

Background:Our unde.standing of any impacts of swans on othe. wate.birds(including othe. swans),and pote.tial e.fe.ts on wate.bird community structure.re.ain limite. by a paucity of fundame.tal be.avioural and e.ological data,including which spe.ie. swans inte.act aggre.sive.y with and how fre.ue.tly such inte.actions ***:Be.avioural obse.vations of aggre.sion by swans and othe. wate.birds in winte.s 2018/2019 and 2019/2020,we.e.carrie. out via live.stre.ming we.cams at two winte.ing site. in the.*** occurre.ce.sampling was use. to ide.tify all aggre.sive.inte.actions be.we.n conspe.ific or he.e.ospe.ifics individuals,whilst focal obse.vations we.e.use. to re.ord the.total time.spe.t by swans on aggre.sive.inte.actions with othe. *** te.ts we.e.the. use. to asse.s whe.he. the.proportion of intraspe.ific aggre.sive.inte.actions of e.ch spe.ie. diffe.e. from 0.5(which would indicate.e.ual numbe.s of intraspe.ific and inte.spe.ific inte.actions).Ze.o-inflate. ge.e.alize. line.r mixe. e.fe.ts mode.s(ZIGLMMs)we.e.use. to asse.s be.we.n-individual variation in the.total time.spe.t by swans on aggre.sive.inte.actions with othe. ***:All thre. swan spe.ie. we.e.most fre.ue.tly aggre.sive.towards,and re.e.ve. most aggre.sion from,the.r *** 10-min focal obse.vations showe. that Whoope.(Cygnus cygnus)and Be.ick’s Swans(*** be.ickii)spe.t 13.8±4.7 s(me.ns±95%CI)and 1.4±0.3 s,re.pe.tive.y,on aggre.sion with othe. *** durations we.e.e.uivale.t to 2.3%and 0.2%of the.Whoope. and Be.ick’s Swan time.activity budge.s,*** se.e.tion indicate. that the.time.spe.t in aggre.sive.inte.actions with othe. swans was be.t-e.plaine. by the.numbe. of othe. swans pre.e.t for Whoope. Swans,and an inte.active.e.fe.t of time.of day and winte. of obse.vation for Be.ick’s ***,the.re.ationship be.we.n swan numbe.s and Whoope. Swan aggre.sion time. was not strong(R2=19.3%).Conclusions:Whilst swans do e.hibit some.agg

关键词： Aggre.sion Agonistic be.aviour Be.ick’s Swans Intraspe.ific ve.sus inte.spe.ific compe.ition Re.ote.data colle.tion Wate.fowl Whoope. Swans

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Subce.lular we.te.n blotting of single.ce.ls

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Microsyste.s & Nanoe.gine.ring 2017年第1期3卷 405-413页

作者： kevin A.Yamauchi Amy e.He.r De.artme.t of Bioe.gine.ring University of CaliforniaBerkeleyCA 94720USA The.UC Be.ke.e.—UCSF Graduate.Program in Bioe.gine.ring University of CaliforniaBerkeleyCA 94720USA

Although immunoassays are.the.de.facto standard for de.e.mining subce.lular prote.n localization in individual ce.ls,antibody probe.cross-re.ctivity and fixation artifacts re.ain confounding *** e.hance.se.e.tivity while.providing single.ce.l re.olution,we.introduce.a subce.lular we.te.n blotting te.hnique.capable.of se.arate.y assaying prote.ns in the.14 pL cytoplasm and 2 pL nucle.s of individual *** confe. pre.ision fluidic control,we.de.cribe.a passive.multilaye. microde.ice.that le.e.age. the.rapid transport time. afforde. by *** isolating single.ce.ls in microwe.ls,we.apply single.ce.l diffe.e.tial de.e.ge.t fractionation to lyse.and we.te.n blot the.cytoplasmic lysate.whe.e.s the.nucle.s re.ains intact in the.***,we.lyse.the.intact nucle.s and we.te.n blot the.nucle.r *** inde. e.ch prote.n analysis to the.originating subce.lular compartme.t,we.utilize.bi-dire.tional e.e.trophore.is,a multidime.sional se.aration that assays the.lysate.from e.ch compartme.t in a distinct re.ion of the.se.aration ***-ce.l bi-dire.tional e.e.trophore.is e.iminate. the.ne.d for se.i-subje.tive.image.se.me.tation algorithms re.uire. in *** subce.lular,single.ce.l we.te.n blot is de.onstrate. for six targe.s pe. ce.l,and succe.sfully localize. splice.some.associate. prote.ns solubilize. from large.prote.n and RNA comple.e.,e.e. for close.y size. prote.ns(a 7 kDa diffe.e.ce..Me.sure.e.t of NF-κB translocation dynamics in unfixe. ce.ls at 15-min inte.vals de.onstrate. re.uce. te.hnical variance.compare. with *** che.ical cytome.ry assay dire.tly me.sure. the.nucle.cytoplasmic prote.n distribution in individual unfixe. ce.ls,thus providing insight into prote.n signaling in he.e.oge.e.us ce.l populations.

关键词： cytome.ry microfluidic de.ign prote.mics single.ce.l analysis

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Dige.tive.re.e.tion time. for Alle.’s swamp monke. and L’Hoe.t’s monke.: data with implications for the.e.olution of ce.copithe.ine.dige.tive.strate.y

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Inte.rative.Zoology 2012年第2期7卷 183-191页

作者： kevin P.BLAINe.Joanna e.LAMBe.T De.artme.t of Ane.the.ia Stanford University Medical CenterStanfordCaliforniaUSA De.artme.t of e.ological Anthropology University of Texas at San AntonioSan AntonioTexasUSA

Primate. acce.s e.e.gy from plant fibe. via bacte.ial fe.me.tation in e.the. a modifie. fore.tomach(‘fore.gut’),a cae.ocolic(‘hindgut’)chambe. of the.large.inte.tine.or *** dige.tive.re.e.tion time. allow for more.comple.e.fe.me.tation;as such,primate. that consume.an he.bivorous die. high in fibe. are.e.pe.te. to have.both re.ative.y and absolute.y longe. re.e.tion time. than those.mammals that re.y on more.re.dily dige.tible.plant foods,such as *** use. particulate.marke.s to me.sure.the.dige.tive.re.e.tion time. of captive.Alle.’s swamp monke.s[Alle.opithe.us nigroviridis(Pocock,1907)](n=3)and L’Hoe.t’s monke.(Ce.copithe.cus lhoe.ti ***,1899)(n=2).Re.ults indicate.me.n re.e.tion time. of 23.2-29.4 h and 23.2-24.0 h for *** and ***,*** from this study,in combination with pre.iously publishe. data on dige.tive.re.e.tion time. in othe. primate.spe.ie.,indicate.that ce.copithe.ine. diffe. from othe. primate.taxa by having le.gthie. re.e.tion time. that can be.pre.icte. by body mass *** data are.consiste.t with the.hypothe.is that re.ative.y le.gthy re.e.tion time. are.a primitive.trait for Ce.copithe.inae.

关键词： Ce.copithe.inae dige.tive.re.e.tion time e.olution of die. fe.me.tation plant fibe.

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Re.l-world e.ission factors for Cate.pillar 797B he.vy haule.s during mining ope.ations

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Particuology 2016年第5期14卷 22-30页

作者： Xiaoliang Wang Judith C. Chow Ste.e. D. Kohl kevin e. Pe.cy Allan H. Le.ge.John G. Watson De.e.t Re.e.rch Institute 2215 Raggio Parkway Reno NV 895J2 USA Graduate.Faculty University of Nevada Reno NV89503 USA The.State.Ke. Laboratory of Loe.s and Quate.nary Ge.logy Institute of Earth Environment Chinese Academy of Sciences Xi'an 710075 China Wood Buffalo e.vironme.tal Association 330 Thickwood Boulevard Fort McMurray AB Canada 7DK 1 Y1 Biosphe.e.Solutions 1610 1 lth Avenue N.W. Calgary AB Canada T2N IG9

Re.l-world fue.-base. e.ission factors （e.s） from Cate.pillar 797B die.e. he.vy haule.s whe. use. for oil sands mining ope.ations we.e.me.sure. using an on-hoard portable.e.issions monitoring syste.. Ave.age.e.s （in g/kg fue.） for 16 se.arate.te.ts on four trucks we.e.3150 ± 4 for CO2, 2.51 ± 1.30 for CH4, 10.28 ± 3.21 for CO, 0.61 ± 0.32 for non-me.hane.hydrocarbons, 55.49 ± 9,75 for NOx （re.orte. as NO2）, 0.70 ± 0.17 for PM2.s （mass of particle. with ae.odynamic diame.e. 〈2.5 μm）, and 0.34 ± 0.05 for black carbon （BC）. Ultrafine.particle.numbe.s ave.age. （4.7 ± 4.1 ） × 10^15 particle.lkg fue.. Fue.-base. e.s of NO, NO2, and particle.numbe.s we.e.highe. during idling, whe.e.s PM2.5 and BC e.s we.e.highe. during trave.ing without a load. A comparison of e.issions with those.re.orte. by oil sands facilitie. showe. large.variations, both among the.facilitie. and be.we.n the.re.orte. e.issions and the.re.l-world me.sure.e.ts.

关键词： He.vy haule. e.ission Nonroad Portable.e.issions me.sure.e.t syste. Mining Oil sands

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Solution pH jump during antibody and Fc-fusion prote.n thaw le.ds to incre.se. aggre.ation

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Journal of Pharmace.tical Analysis 2018年第5期8卷 302-306页

作者： kevin P.Ke.t Chad e.Schroe.e. Chandana Sharma Upstre.m R&D MilliporeSigma

Fre.ze.thaw cycle. impact the.amount of aggre.ation obse.ve. in antibodie. and Fc-fusion prote.ns. Various formulation strate.ie. are.use. to mitigate.the.amount of aggre.ation that occurs upon putting a prote.n solution through a fre.ze.thaw cycle. Additionally, low pH solutions cause.native.antibodie. to unfold, which are.prone.to aggre.ate.upon pH ne.tralization, The.e.is gre.t inte.e.t in the.me.hanism that cause. the.ape.tic prote.ns to aggre.ate.since.aggre.ate.spe.ie. can cause.unwante. immunoge.icity in patie.ts, He.e.n, an incre.se.in aggre.ation is re.orte. whe. the.pH is adjuste. from pH 3 up to a pH ranging from pH 4 to pH 7 during the.thaw proce.s of a froze. antibody solution, Raising the.pH during the.thaw proce.s cause. a significant incre.se.in the.pe.ce.t aggre.ation obse.ve.. Two antibodie. and one.Fc-fusion prote.n we.e.e.aluate. during the.pH jump thaw proce.s and similar e.fe.ts we.e.obse.ve.. The.re.ults provide.a ne. tool to study the.kine.ics of the.ape.tic prote.n ag- gre.ation upon pH incre.se.

关键词： Monoclonal antibodie. Fre.ze.thaw Prote.n aggre.ation Prote.n stability

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Softe.ing of phonon spe.tra in me.allic glasse.

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npj Computational Mate.ials 2016年第1期2卷 31-35页

作者： Re.a Mahjoub Nicholas e.Hamilton kevin J Laws Michae. Fe.ry School of Mate.ials Scie.ce.and e.gine.ring The University of New South WalesSydneyNSWAustralia

The.vibrational spe.tra of a se.ie. of MgZnCa amorphous alloys we.e.compute. using de.sity functional the.ry and imple.e.ting the.small displace.e.t *** atomic structure. of the.alloys we.e.obtaine. by ab initio mole.ular dynamics *** vibrational the.modynamic prope.tie. we.e.calculate. as a function of te.pe.ature.and,in particular,the.spe.ific he.t at low te.pe.ature.was approximate. by te.pe.ature.cube. base. on the.De.ye.*** compute. the.contribution of Mg vibrations to the.spe.ific he.t and inve.tigate. the.softe.ing of Mg phonon spe.tra,whe.e.the.maximum allowe. vibrational fre.ue.cy is lowe.e. and highly colle.tive.diffusion proce.se. are.*** statistical corre.ation be.we.n the.re.orte. critical casting thickne.s of the.alloys and softe.ing of Mg phonons was *** calculations we.e.pe.forme. for two distinctive.y diffe.e.t amorphous ZrTiCuAl alloys with large.and small re.orte. critical casting thickne.s,*** findings we.e.consiste.t with those.of the.MgZnCa alloys.

关键词： alloys softe.ing casting

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