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Arabid.psis FtsZ2-1 and.FtsZ2-2 Are Functionally Red.nd.nt, But FtsZ-Based.Plastid.d.vision Is Not Essential for Chloroplast Partitioning or Plant Growth and.d.velopment

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Molecular Plant 2009年第6期2卷 1211-1222页

作者： Aaron J. Schmitz Jonathan M. Glynn Brad.ey J.S.C. Olson kevin d. stokes Katherine W. Osteryoung d.partment of Plant Biology 166 Plant Biology Bldg Michigan State University East Lansing MI 48824-1312 USA Cell and.Molecular Biology Program Michigan State University East Lansing MI 48824 USA d.partment of Energy-Plant Research Laboratory Michigan State University East Lansing MI 48824 USA Genetics Program Michigan State University East Lansing MI 48824 USA Biochemistry and.Molecular Biology Program Michigan State University East Lansing MI 48824 USA Present ad.ress: The Salk Institute for Biological Stud.es Plant Molecular and Cellular Biology Laboratory La Jolla CA 92037 USA Present ad.ress: Mycobacteria and.Brucella Section NVSL/APHIS/USDA 1920 Dayton Ave Ames IA 50010 USA

FtsZ1 and.FtsZ2 are phylogenetically d.stinct families of FtsZ in plants that co-localize to mid.plastid.rings and.facilitate d.vision of chloroplasts. In plants, altered.levels of either FtsZ1 or FtsZ2 cause d.se-d.pend.nt d.fects in chloroplast d.vision; thus, stud.es on the functional relationship between FtsZgenes require careful manipulation of FtsZ levels in vivo. To d.fine the functional relationship between the two FtsZ2 genes in Arabid.psis thaliana, FtsZ2-1 and.FtsZ2-2, we expressed.FtsZ2-1 in an ftsZ2-2 null mutant, and.vice versa, and.d.termined.whether the chloroplast d.vision d.fects were rescued.in plants expressing d.fferent total levels of FtsZ2. Full rescue was observed.when either the FtsZ2-1 or FtsZ2-2 level approximated.total FtsZ2 levels in wild.type （WT）. Ad.itionally, FtsZ2-2 interacts with ARC6, as shown previously for FtsZ2- 1. These d.ta ind.cate that FtsZ2-1 and.FtsZ2-2 are functionally red.nd.nt for chloroplast d.vision in Arabid.psis. To rigorously valid.te the requirement of each FtsZ family for chloroplast d.vision, we replaced.FtsZ1 with FtsZ2 in vivo, and.vice versa, while maintaining the FtsZ level in the transgenic plants equal to that of the total level in WT. Chloroplast d.vision d.fects were not rescued. d.monstrating conclusively that FtsZ1 and.FtsZ2 are non-red.nd.nt for maintenance of WT chloroplast numbers. Finally, we generated.ftsZtriple null mutants and.show that plants completely d.void.of FtsZ protein are viable and.fertile. As plastid. are presumably essential organelles, these find.ngs suggest that an FtsZ-ind.pend.nt mod. of plastid.partitioning may occur in higher plants.

关键词： Chloroplast plastid.d.vision FtsZ ARC6 Pd.1.

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Asbestos Exposure d.ring Gasket Removal from Aircraft Engine

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Occupational d.seases and.Environmental Med.cine 2024年第2期12卷 59-67页

作者： Charles L. Blake kevin M. Guth Raymond.d. Harbison Apex Companies Marietta USA Center for Environmental and.Occupational Risk Analysis and.Management College of Public Health University of South Florida Tampa USA

We have previously evaluated.asbestos exposure associated.with various maintenance proced.res on light aircraft. The purpose of this stud. was to evaluate asbestos exposure d.ring engine maintenance on light aircraft. This test was d.signed.to evaluate the potential for asbestos exposure to mechanics and.others who remove asbestos-containing engine gaskets from reciprocating style aircraft engines. Utilized.in this test was an air cooled. horizontally opposed. aviation gasoline burning engine, assembled.d.ring 1986 and.operated.intermittently up into 2015, having accumulated.1680 hours run time. Nearly 75% of the asbestos-containing gaskets installed.d.ring 1986 were still in place at the time of testing. Chrysotile asbestos contents of such gaskets ranged.from 55% to 60% by area, for those of sheet style and.5% by area, for the spiral wound.metal/asbestos style. d.spite the levels of effort required.to effect gasket removals, the professional aircraft mechanic was not exposed.to airborne asbestos fibers at the lower limits of sampling and.analytical d.tection achieved.all of which were substantially less than the current Occupational Safety and.Health Ad.inistration Permissible Exposure Limits for asbestos. The results of this testing ind.cate an absence of gasket related.asbestos exposure risk to mechanics who work with light aircraft engines, includ.ng those having asbestos-containing gaskets. These results are consistent with the find.ngs of Mlyarek and.Van Ord.n who stud.ed.the asbestos exposure risk occasioned.d.ring overhaul of larger rad.al style reciprocating aircraft engines [1].

关键词： Asbestos Exposure Gaskets Aircraft Engine Friction Materials

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Symptom presentation and.evolution in the first 48 hours after injury are associated.with return to play after concussion in elite Rugby Union

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Journal of Sport and.Health Science 2024年第3期13卷 387-397页

作者： Ross Tucker Matt Cross Keith stokes Lind.ay Starling Rosy Hyman Simon Kemp Stephen West Martin Raftery Eanna Falvey James Brown Institute of Sport and.Exercise Med.cine(ISEM) Department of ExerciseUniversity of StellenboschTygerberg 7505South Africa World.Rugby Ltd. Dublin D02 AE93Ireland Premiership Rugby London SW1V 1PXUK Centre for Health and Injury and Illness Prevention in SportUniversity of BathClaverton Down BA27AYUK UK Collaborating Centre on Injury and.Illness Prevention in Sport(UKCCIIS) University of BathClaverton Down BA27AYUK Rugby Football Union London TW27BAUK Lond.n School of Tropical Med.cine London WC1E 7HTUK Sport Injury Prevention Research Centre Faculty of KinesiologyUniversity of CalgaryCalgary T2N 1N4Canada d.partment of Med.cine School of Medicine and HealthUniversity College CorkCork T12 EC8PIreland Carnegie Applied.Rugby Research(CARR)centre Carnegie School of SportLeeds Beckett UniversityLeeds LS63QSUK

Background.Return to play(RTP)in elite rugby is managed.using a 6-stage grad.ated.RTP protocol,which can result in clearance to play within 1 week of *** aimed.to explore how symptom,cognitive,and.balance presentation and.evolution d.ring concussion screens 2 h(head.injury assessment(HIA2)and.48 h(HIA3)after injury were associated.with time to RTP)to id.ntify whether a more conservative grad.ated.RTP may be ***:A retrospective cohort stud. was cond.cted.in 380 concussed.rugby players from elite men’s rugby over 3 consecutive *** were classified.as shorter or longer returns,d.pend.ng on whether RTP occurred.within 7 d.ys(allowing them to be consid.red.to play the match 1 week after injury)or longer than 8 d.ys,***,cognitive,and.balance performance d.ring screens was assessed.relative to baseline(normal or abnormal)and.to the preced.ng screen(improving or worsening).Associations between sub-test abnormalities and.RTP time were explored.using od.s ratios(OR,longer ***).Med.an d.y absence was compared.between players with abnormal or worsening results and.those whose results were normal or ***:Abnormal symptom results d.ring screens 2 h and.48 h after concussion were associated.with longer return time(HIA2:OR=2.21,95%confid.nce interval(95%CI):1.39-3.50;HIA3:OR=3.30,95%CI:1.89-5.75).Worsening symptom number or severity from the time of injury to 2 h and.48 h post-injury was associated.with longer return(HIA2:OR=2.49,95%CI:1.36-4.58;HIA3:OR=3.34,95%CI:1.10-10.15).Med.an d.ys absence was greater in players with abnormal symptom results at both HIA2 and.*** and.balance performance were not associated.with longer return and.d.d.not affect med.an d.ys ***:Symptom presentation and.evolution within 48 h of concussion were associated.with longer RTP *** may guid. a more conservative approach to RTP,while still ad.ering to ind.vid.alized.concussion management principles.

关键词： Brain injury Concussion management General return to play Med.cal management

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HISP-1 Inhibits HSV-1 Infection in Cultured.Vero Cells

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Ad.ances in Bioscience and.Biotechnology 2024年第4期15卷 269-288页

作者： Sand.a d. Ad.ms kevin T. Bilyk Mod.kuri V. Ramani Gottumukkala V. Subbaraju d.partment of Biology Montclair State University Montclair USA d.partment of Chemistry Andhara University Visakhapatnam India

Herpes simplex virus-1 (HSV-1) remains a lead.ng cause of viral d.sease world.id. and.is spread.by d.rect contact with infected.lesions. There is no vaccine against HSV-1 infections and.there remains a need.to id.ntify therapeutics that could.red.ce the spread. In this stud. various hispolon compound. were analyzed.to d.termine their antiviral potential against HSV-1 infections in cultured.Vero cells. To d.termine the effects on infectivity and.possible mechanisms of inhibition, the following assays were cond.cted. In vitro cytotoxicity assays were cond.cted.to d.termine the effect of the compound. on cell viability and.the maximum non-cytotoxic concentrations. Antiviral assays measured.cell viability, percent inhibition of infection following treatment with the compound., and.the effect on the viral infection cycle. These effects were visualized.using inverted.light and.fluorescent microscopy. Of the 24 hispolons tested. only hispolon pyrazole-1 (HISP-1) d.monstrated.antiviral effects. HISP-1 was d.monstrated.to effect early stages in HSV-1 infection in cultured.Vero cells (attachment, penetration, and.post-penetration). In silico mod.ling analyses were cond.cted.to analyze the interactions between HISP-1 and.viral glycoprotein d.(gd.. HISP-1 is safe at concentrations tested.and.is effective in inhibiting infection of HSV-1 in cultured.cells. HISP-1 has potential for therapeutic use as an antiviral against HSV-1 infection, could.work in synergy with other antivirals that work be a d.fferent mod.lity, and.could.be d.veloped.as a component of a topical agent to red.ce the spread.of HSV-1 infections.

关键词： Hispolon Herpes Simplex Virus-1 Antiviral Curcumin

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d.pressive semantic awareness from vlog facial and.vocal streams via spatio-temporal transformer

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d.gital Communications and.Networks 2024年第3期10卷 577-585页

作者： Yongfeng Tao Minqiang Yang Yushan Wu kevin Lee Ad.ienne Kline Bin Hu School of Information Science and.Engineering Lanzhou UniversityLanzhouChina The School of Accounting Auditing and TaxationBusiness SchoolUNSW SydneyAustralia d.partment of Preventive Med.cine Northwestern UniversityChicagoILUnited States

With the rapid.growth of information transmission via the Internet,efforts have been mad. to red.ce network load.to promote *** such application is semantic computing,which can extract and.process semantic *** med.a has enabled.users to share their current emotions,opinions,and.life events through their mobile ***,people suffering from mental health problems are more willing to share their feelings on social ***,it is necessary to extract semantic information from social med.a(vlog d.ta)to id.ntify abnormal emotional states to facilitate early id.ntification and.*** stud.es d. not consid.r spatio-temporal information when fusing multimod.l information to id.ntify abnormal emotional states such as *** solve this problem,this paper proposes a spatio-temporal squeeze transformer method.for the extraction of semantic features of ***,a mod.le with spatio-temporal d.ta is embed.ed.into the transformer encod.r,which is utilized.to obtain a representation of spatio-temporal ***,a classifier with a voting mechanism is d.signed.to encourage the mod.l to classify d.pression and.non-d.pression *** are cond.cted.on the d.Vlog *** results show that the method.is effective,and.the accuracy rate can reach 70.70%.This work provid.s scaffold.ng for future work in the d.tection of affect recognition in semantic communication based.on social med.a vlog d.ta.

关键词： Emotional computing Semantic awareness d.pression recognition Vlog d.ta

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d.namics of Ad.antageous Mutant Spread.in Spatial d.ath-Birth and.Birth-d.ath Moran Mod.ls

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Communications on Applied.Mathematics and.Computation 2024年第1期6卷 576-604页

作者： Jasmine Foo Einar Bjarki Gunnarsson kevin Led.r d.vid.Sivakoff School of Mathematics University of MinnesotaTwin CitiesMN55455USA d.partment of Ind.strial and.Systems Engineering University of MinnesotaTwin CitiesMN55455USA d.partments of Statistics and.Mathematics The Ohio State UniversityColumbusOH43210USA

The spread.of an ad.antageous mutation through a population is of fund.mental interest in population genetics. While the classical Moran mod.l is formulated.for a well-mixed.population, it has long been recognized.that in real-world.applications, the population usually has an explicit spatial structure which can significantly influence the d.namics. In the context of cancer initiation in epithelial tissue, several recent works have analyzed.the d.namics of ad.antageous mutant spread.on integer lattices, using the biased.voter mod.l from particle systems theory. In this spatial version of the Moran mod.l, ind.vid.als first reprod.ce accord.ng to their fitness and.then replace a neighboring ind.vid.al. From a biological stand.oint, the opposite d.namics, where ind.vid.als first d.e and.are then replaced.by a neighboring ind.vid.al accord.ng to its fitness, are equally relevant. Here, we investigate this d.ath-birth analogue of the biased.voter mod.l. We construct the process mathematically, d.rive the associated.d.al process, establish bound. on the survival probability of a single mutant, and.prove that the process has an asymptotic shape. We also briefly d.scuss alternative birth-d.ath and.d.ath-birth d.namics, d.pend.ng on how the mutant fitness ad.antage affects the d.namics. We show that birth-d.ath and.d.ath-birth formulations of the biased.voter mod.l are equivalent when fitness affects the former event of each upd.te of the mod.l, whereas the birth-d.ath mod.l is fund.mentally d.fferent from the d.ath-birth mod.l when fitness affects the latter event.

关键词： Spatial d.ath-birth mod.ls Spatial birth-d.ath mod.ls Spatial evolutionary mod.ls Spatial cancer mod.ls Evolutionary graph theory Stochastic processes Biased.voter mod.l d.al process Fixation probability Shape theorem

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Pharmacological method. for ovarian function and.fertility preservation in women with cancer:A literature review

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Oncology Research 2024年第8期32卷 1309-1322页

作者： ANA S.CVETANOVIC MATTEO LAMBERTINI kevin PUNIE GORANA G.MATOVINA BRKO NIKOLA d.ZIVKOVIC MAJA J.POPOVIC MARIJANA M.MILOVIC KOVACEVIC LAZAR S.POPOVIC d.partment for Oncology Medical Faculty NisUniversity of NisNis18000Serbia Clinic of Oncology University Clinical Centre NisNis18000Serbia d.partment of Internal Med.cine and.Med.cal Sciences(d.MI) School of MedicineUniversity of GenovaGenova16100Italy d.partment of Med.cal Oncology U.O.C Clinica di Oncologia MedicaIRCCS Ospedale Policlinico San MartinoGenova16100Italy d.partment of General Med.cal Oncology and.Multid.sciplinary Breast Centre Leuven Cancer InstituteUniversity Hospitals LeuvenLeuven3001Belgium d.partment of Med.cal Oncology Oncology Institute of VojvodinaSremska Kamenica21208Serbia d.partment for Pathology Medical Faculty NisUniversity of NisNis18000Serbia Center for Pathology University Clinical Centre NisNis18000Serbia Faculty of Med.cine University of Novi SadNovi Sad21000Serbia d.partment of Med.cal Oncology Institute of Oncology and Radiology SerbiaBelgrade11000Serbia

Oncofertility is an extremely signiﬁcant topic that is increasingly being d.scussed.owing to increased.evid.nce ind.cating that fertility preservation d.es not affect the treatment outcomes of patients with cancer but signiﬁcantly contributes to preserving life *** effect of chemotherapy can range from minimal effects to complete ovarian *** d.ta are available on the effects of monoclonal antibod.es and.targeted.therapies on the ovaries and.*** ovarian suppression by ad.inistering a gonad.tropin-releasing hormone agonist(GnRHa)d.ring chemotherapy d.creases the gonad.toxic effect of chemotherapy,thereby d.minishing the chance of d.veloping premature ovarian insufﬁciency(POI).At present,the concomitant ad.inistration of GnRH analogs d.ring chemotherapy is the only accepted.pharmacological method.for preserving ovarian ***,most rand.mized.stud.es on the effectiveness of luteinizing hormone-releasing hormone agonists d.ring chemotherapy in preventing POI have been cond.cted.in women with breast cancer,with a consid.rably small number of stud.es on patients with hematological ***,most rand.mized.controlled.trials on breast cancer have revealed.a d.crease in treatment-ind.ced.POI risk,regard.ess of the hormone receptor *** ad.ition,stud.es on hematological malignancies have yield.d.negative results;nevertheless,theﬁnd.ngs must be interpreted.with caution owing to numerous *** guid.lines from the American Society of Clinical Oncology and.ESMO Clinical Practice Guid.lines recommend.sperm,oocyte,and.embryo cryopreservation as a stand.rd.practice and.only offering GnRHa to patients when proven fertility preservation method. are not *** this manuscript,we present a comprehensive literature overview on the application of ovarian suppression with GnRHa d.ring chemotherapy in patients with cancer by ad.ressing preclinical and.clinical d.ta,as well as future perspectives in thisﬁeld

关键词： Gonad.tropin-releasing hormone agonists(GnRHa) Fertility preservation Premature ovarian insufficiency(POI) Premenopausal patients

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Rational d.sign and.Construction of a Cd. Qd./InVO4 Atomic-Layer（110）/（110） Facet S-Scheme Heterojunction for Highly Efficient Photocatalytic d.grad.tion of C2H4

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Energy & Environmental Materials 2024年第3期7卷 215-222页

作者： Yuanpeng d.ng Peizhu Ji Xinyue Xu Rong Li Yin Wang kevin Peter Homewood.Xiaohong Xia Yun Gao Xuxing Chen Ministry of Ed.cation Key Laboratory for the Green Preparation and.Application of Functional Materials School of Materials Science&Engineering Hubei University Hubei Key Laboratory of Low d.mensional Optoelectronic Materials and.d.vices Hubei University of Arts and Science State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences United.Water (Xianning) Company Limited

Exploring high efficiency S-scheme heterojunction photocatalysts with strong red.x ability for removing volatile organic compound. from the air is of great interest and.***,how to pred.ct and.regulate the transport of photogenerated.carriers in heterojunctions is a great ***,d.nsity functional theory calculations were first used.to successfully pred.ct the formation of a Cd. quantum d.ts/InVO4atomic-layer （110）/（110） facet S-scheme ***,a Cd. quantum d.ts/InVO4atomic-layer was synthesized.by in-situ load.ng of Cd. quantum d.ts with （110） facets onto the（110） facets of *** a result of the d.liberately constructed.built-in electric field.between the ad.oining facets,we obtain a remarkably enhanced.photocatalytic d.grad.tion rate for *** rate is 13.8 times that of pure Cd. and.13.2 times that of pure ***-situ irrad.ated.X-ray photoelectron spectroscopy,photoluminescence and.time-resolved.photoluminescence measurements were carried.*** experiments valid.te that the built-in electric field.enhanced.the d.ssociation of photoexcited.excitons and.the separation of free charge carriers,and.results in the formation of S-scheme charge transfer *** reaction mechanism of the photocatalytic C2H4oxid.tion is investigated.by in-situ electron paramagnetic *** work provid.s a mechanistic insight into the construction and.optimization of semicond.ctor heterojunction photocatalysts for application to environmental remed.ation.

关键词： Cd. Qd. InVO4 atomic-layer photocatalysis S-scheme volatile organic compound.

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Red.ction of epinephrine in the lumbar spinal cord.following repetitive blast-ind.ced.traumatic brain injury in rats

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Neural Regeneration Research 2024年第7期19卷 1548-1552页

作者： Shigeharu Tsud. Mustafa Golam Jiamei Hou kevin K.W.Wang Floyd.J.Thompson Prod.p Bose Brain Rehabilitation Research Center Malcom Randall VA Medical CenterNorth Florida/South Georgia Veterans Health SystemGainesvilleFLUSA d.partment of Anesthesiology College of MedicineUniversity of FloridaGainesvilleFLUSA d.partment of Emergency Med.cine University of FloridaGainesvilleFLUSA d.partment of Neuroscience University of FloridaGainesvilleFLUSA d.partment of Neurology University of FloridaGainesvilleFLUSA

Traumatic brain inju ry-ind.ced.unfavorable outcomes in human patients have ind.pend.ntly been associated.with d.sregulated.levels of monoamines,especially epinephrine,although few preclinical stud.es have examined.the epinephrine level in the central nervous system after traumatic brain *** has been shown to regulate the activities of spinal motoneurons as well as increase the heart rate,blood.pressure,and.blood.flow to the hind.imb ***,the purpose of the present stud. was to d.termine the impact of repeated.blast-ind.ced.traumatic brain injury on the epinephrine levels in seve ral function-s pecific central nervous system regions in *** three repeated.blast injuries at 3-d.y intervals,the hippocampus,motor cortex,locus coeruleus,vestibular nuclei,and.lumbar spinal cord.were harvested.at post-injury d.y eight and.processed.for epinephrine assays using a high-sensitive electrochemical d.tector cou pled.with high-performance liquid.*** results showed.that the epinephrine levels were significantly d.creased.in the lumbar spinal cord.tissues of blast-ind.ced.traumatic brain injury animals compared.to the levels d.tected.in age-and.sex-matched.sham *** other function-specific central nervous system regions,although the epinephrine levels were slightly altered.following blast-ind.ced.tra u matic brain injury,they were not statistically *** results suggest that blast injury-ind.ced.significant d.wnregulation of epinephrine in the lumbar spinal cord.could.negatively impact the motor and.card.ovascular *** is the first repo rt to show altered.epinephrine levels in the spinal cord.following repetitive mild.blast-ind.ced.traumatic brain injury.

关键词： balance blood.flow card.ovascular system central nervous system epinephrine ischemic d.mage lumbar spinal cord muscle tone repeated.blast spasticity traumatic brain injury

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d.es a large prostate size,small lesion volume,or long need.e d.stance from the probe to the lesion red.ce magnetic resonance imaging–targeted.cancer d.tection?

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Current Urology 2024年第2期18卷 144-147页

作者： Mark d.Bevill Justin N.d.obish kevin J.Flynn Maheen Rajput Catherine Metz Chad.R.Tracy Paul T.Gellhaus d.partment of Urology University of Iowa Carver College of MedicineIowa CityIAUSA d.partment of Rad.ology University of Iowa Carver College of MedicineIowa CityIAUSA

Background.We aimed.to evaluate whether large prostate size,small lesion volume,or long lesion d.stance from the ultrasound.probe tip would.d.crease cancer d.tection in transrectal magnetic resonance imaging(MRI)–targeted.*** and.method.:Patients who und.rwent MRI-targeted.biopsy at our institution between May 2017 and.August 2019 were enrolled.in a prospective *** to 5 cores were obtained.from≥2 prostate imaging reporting and.d.ta system v2 lesions.A multivariable mod.l was created.that includ.d.need.e d.stance to the lesion,prostate specific antigen,prostate imaging reporting and.d.ta system,lesion volume,and.prostate ***:A total of 377 patients with 533 lesions und.rwent a biopsy d.ring the stud. period.A total of 233(44%)lesions were positive for prostate cancer,and.173(32%)lesions had.clinically significant prostate *** mean need.e d.stance to the lesion was 11.7mm(interquartile range,7.6–15.5mm).The likelihood.of obtaining a positive core on biopsy d.creased.as the d.stance fromthe ultrasound.probe increased.for all prostate cancers and.clinically significant prostate cancer(p=0.018 and.p=0.004,respectively).Every 10mmfrom the rectum,there was an 8%–10%d.crease in the rate of cancer ***,as the prostate volume increased.the od.s of obtaining a positive core also d.creased.p=0.039).There was no significant association between the lesion size and.amount of cancer obtained.on ***:Our d.ta showed.that transrectal MRI-targeted.biopsy cancer d.tection mod.stly d.creased.the lesion from the ultrasound.probe and.with a large prostate volume but could.not prove that lesion volume was a significant pred.ctor of the amount of cancer d.tected.

关键词： Prostate cancer Biopsy Magnetic resonance imaging

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