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MiR-130a regulates and dendritic spine MeCP2 neurite outgrowth density by targeting

Protein & Cell 2016年第7期7卷 489-500页

作者： Yunjia Zhang Mengmeng Chen Zilong Qiu keping hu Warren McGee Xiaoping Chen Jianghong Liu Li Zhu Jane Y. Wu State Key Laboratory for Brain & Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory of Molecular Basis of Neural Plasticity Institute of Neuroscience Chinese Academy of Sciences Shanghai 200031 China Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences Beijing 100193 China Department of Neurology Center for Genetic Medicine Lurie Cancer Center Northwestern University Feinberg School of Medicine Chicago IL 60611 USA

MicroRNAs （miRNAs） are critical for both development and function of the central nervous system. Significant evidence suggests that abnormal expression of miRNAs is associated with neurodevelopmental disorders. MeCP2 protein is an epigenetic regulator repressing or activating gene transcription by binding to methylated DNA. Both loss-of-function and gain-of-function muta- tions in the MECP2 gene lead to neurodevelopmental disorders such as Rett syndrome, autism and MECP2 duplication syndrome. In this study, we demonstrate that miR-130a inhibits neurite outgrowth and reduces dendritic spine density as well as dendritic complexity. Bioinformatics analyses, cell cultures and biochemical experiments indicate that miR-130a targets MECP2 and down-regulates MeCP2 protein expression. Further- more, expression of the wild-type MeCP2, but not a loss- of-function mutant, rescues the miR-130a-induced phe- notype. Our study uncovers the MECP2 gene as a pre- vious unknown target for miR-130a, supporting that miR-130a may play a role in neurodevelopment by reg- ulating MeCP2. Together with data from other groups,our work suggests that a feedback regulatory mecha- nism involving both miR-130a and MeCP2 may serve to ensure their appropriate expression and function in neural development.

关键词： miR-130a MECP2 neurite outgrowth,dendritic spines dendrite morphology

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Marein Modulates the Methylglyoxal-induced Oxidative Stress Response Via the AMPK/GLUT4 Signaling Pathway in PC12 Cells

Marein Modulates the Methylglyoxal-induced Oxidative Stress ...

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第十四届全国中药和天然药物学术研讨会

作者： Baoping Jiang Lijia Xu Liang Le Wenting Wan keping hu Peigen Xiao Chinese Academy of Medical Science and Peking Union Medical College

＜正＞Diabetic encephalopathy is an emerging diabetic complication,and diabetes-associated cognitive decline is the core component of this *** studies have suggested that methylglyoxal（MG）,a highly reactive metabolite in hyperglycemia,plays an important role in diabetic *** is a chalcone compound from Coreopsis tinctoria,but little

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Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation

Identification of EFHD1 as a novel Ca2+ sensor for mitoflash...

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第十一届全国钙信号和细胞功能研讨会

作者： Tingting Hou Chongshu Jian Jiejia Xu August Yue huang Jianzhong Xi keping hu Liping Wei Heping Cheng Xianhua Wang State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular Medicine Peking-Tsinghua Center for Life Sciences Peking University Center for Bioinformatics State Key Laboratory of Protein and Plant Gene Research School of Life Sciences Peking University Department of Biomedical Engineering College of Engineering Peking University Research Center for Pharmacology and Toxicology Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

Mitochondrial flashes(mitoflashes) represent stochastic and discrete mitochondrial events that each comprises a burst of superoxide production accompanied by transient depolarization and matrix alkalinization in a respiratory mitochondrion. While mitochondrial Ca is shown to be an important regulator of mitoflash activity, little is known about its specific mechanism of action. Here we sought to determine possible molecular players that mediate the Ca regulation of mitoflashes by screening mitochondrial proteins containing the Ca-binding motifs. In silico analysis and targeted si RNA screening identified four mitoflash activators(MICU1, EFHD1, SLC25A23, SLC25A25) and one mitoflash inhibitor(LETM1) in terms of their ability to modulate mitoflash response to hyperosmotic stress. In particular, overexpression or downregulation of EFHD1 enhanced or depressed mitoflash activation, respectively, under various conditions of mitochondrial Ca elevations. Yet, it did not alter mitochondrial Ca handling, mitochondrial respiration, or ROS-induced mitoflash production. Further, disruption of the two EF-hand motifs of EFHD1 abolished its potentiating effect on the mitoflash responses. These results indicate that EFHD1 functions as a novel mitochondrial Ca sensor underlying Ca-dependent activation of mitoflashes.

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快速气相色谱法分析白酒中的香味组分

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色谱 2007年第4期25卷 586-589页

作者：张超胡可萍端裕树曹磊武杰岛津国际贸易(上海)有限公司北京分析中心北京100020 北京化工大学北京100029 中国石油勘探开发研究院北京100080

中国的传统白酒里含有多种香味组分,包括醇类、醛类、酸类和酯类,它们的比率决定着白酒的香型和品质。这些组分可以使用气相色谱仪进行很好的分析并定性和定量。为了缩短分析时间,建立了一种快速检测白酒中香味组分的气相色谱法。采用... 详细信息

中国的传统白酒里含有多种香味组分,包括醇类、醛类、酸类和酯类,它们的比率决定着白酒的香型和品质。这些组分可以使用气相色谱仪进行很好的分析并定性和定量。为了缩短分析时间,建立了一种快速检测白酒中香味组分的气相色谱法。采用该方法,用20m×0.1mm×0.1μm的熔融石英毛细管柱在12min之内完成了对白酒中香味组分的分析,分析时间只是传统色谱方法的三分之一。该方法的重现性良好。

关键词：快速气相色谱香味组分白酒

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齐地工商文化的发展与“一带一路”建设互动关系研究

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管子学刊 2020年第4期 42-47页

作者：姜颖胡克平山东理工大学文学与新闻传播学院山东淄博255000 淄博职业学院招生办公室山东淄博255314

从土著的东夷文明开始,历经姜太公封齐立国、桓管改革,齐地确立了崇商重工传统,形成了纺织、冶炼铸造、玉漆器加工、毛皮、陶瓷等多种具有代表性的传统产业,为“一带一路”建设提供了便利的交通条件、奠定了坚实的资源基础;作为古代丝... 详细信息

从土著的东夷文明开始,历经姜太公封齐立国、桓管改革,齐地确立了崇商重工传统,形成了纺织、冶炼铸造、玉漆器加工、毛皮、陶瓷等多种具有代表性的传统产业,为“一带一路”建设提供了便利的交通条件、奠定了坚实的资源基础;作为古代丝绸之路的重要源头,齐地工商业的发展也为“一带一路”建设提供了经验和借鉴。反之,时至今日,“一带一路”建设对以齐地为主力的山东工商文化的发展提出了更高的、具有时代特点的要求。历史上齐地工商文化的发展对“一带一路”建设的促进,“一带一路”建设对山东工商文化发展的推动,二者之间形成了一种良性的互动关系。

关键词：齐地工商文化丝绸之路一带一路

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MeCP2 in autism-displays allelic expression imbalance and affects both nerve system and heart development through Notch pathway

MeCP2 in autism-displays allelic expression imbalance and af...

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第十四次全国医学遗传学学术会议

作者： Yufang Zheng Yumeng Wang Yahui Liu Yasong Du Zhangmin Yang Yanqing He Xiaohong Gong Xing Liu Jian huang Bing Su keping hu Zilong Qiu Hongyan Wang State Key Laboratory of Genetic Engineering MOE Key Laboratory of Contemporary Anthropologyand Collaborative Innovation Center for Genetics & DevelopmentSchool of Life SciencesFudan University Institute of Developmental Biology &Molecular Medicine Fudan University Shanghai Mental Health Center Shanghai Jiaotong University Shanxi Normal University School of Life Sciences National Engineering Center for Biochip at Shanghai Kunming Institute of Zoology Chinese Academy of Sciences The Institute of Medicinal Plant Development Chinese Academy of Medical Sciences The Institute of Neuroscience Chinese Academy of Sciences

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MeCP2 in autism-displays allelic expression imbalance and affects both nerve system and heart development through Notch pathway

MeCP2 in autism-displays allelic expression imbalance and af...

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2015中国遗传学会大会

作者： Yufang Zheng Yumeng Wang Yahui Liu Yasong Du Zhangmin Yang Yanqing He Xiaohong Gong Xing Liu Jian huang Bing Su keping hu Zilong Qiu Hongyan Wang State Key Laboratory of Genetic Engineering MOE Key Laboratory of Contemporary Anthropologyand Collaborative Innovation Center for Genetics & DevelopmentSchool of Life SciencesFudan University Institute of Developmental Biology & Molecular Medicine Fudan University Shanghai Mental Health Center Shanghai Jiaotong University Shanxi Normal University School of Life Sciences National Engineering Center for Biochip at Shanghai Kunming Institute of Zoology Chinese Academy of Sciences The Institute of Medicinal Plant Development Chinese Academy of Medical Sciences The Institute of Neuroscience Chinese Academy of Sciences

Both Autism and Rett syndrome are neural development disorders sharing certain characterizations such as impaired social interaction or communication,and the restricted and repetitive ***,they have very different sexual bias as autism affects 4-5 boys versus 1 girl while Rett syndrome mainly affects girls with limited *** of MeCP2(Methyl-CpG-binding protein 2),a X chromosome gene,has been identified not only in most Rett syndrome patients,but also in autism *** understand whether MeCP2 contributes to the sexual imbalance in autism,we analyzed the expression of MeCP2 in both Rhesus monkey(Macaca mulatto)and ***,we found that the expression of MeCP2 in brain did not follow XCI(X chromosome inactivation)in both *** using C57B/L6 MeCP2transgenic mouse crossed with FVB WT mouse,allelic expression imbalance(AEI)of MeCP2 in brain was observed when the mutation is from the maternal side but not from the paternal *** might significantly reduce the chance of female offsprings’abnormality due to MeCP2 mutations even in consideration of X chromosome compensation,which in turn contributes to the sexual bias effects in *** further study the functions of MeCP2 in autism,we sequenced 288 autism patients and383 *** rare nonsynonymous mutations(T197M,G232A,H371R,E392K,G426S)were individually identified in autism patients of four boys and one *** analysis in zebrafish showed that those mutations lose the ability to rescue the abnormal defects in both brain and heart caused by MeCP2 morpholinos ***,when overexpressed,those mutations caused more severe defects compared to WT *** using human U251 cells,we found that WT MeCP2 could inhibit the Notch signaling by down-regulating ADAM10 expression whereas those autistic mutants lost the inhibition *** importantly,it was found that the possible underlying molecular mechanism is due to WT MeCP2,not the mutated MeCP2,up-regulat

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Detection of BmNPV multiplication in different tissues of highly resistant silkworm strain NB by RealTime-PCR

Detection of BmNPV multiplication in different tissues of hi...

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华东六省一市生物化学与分子生物学学会2006年学术交流会

作者： Gao Lu,Chen keping,hu ZhiGang,Yao Qing (Institute of Life Sciences, Jiangsu University,Zhenjiang 212013,P. R. China School of medical technology, Jiangsu University, Zhenjiang 212001, P. R. China.)

To determine the mechanism by which highly resistant silkworm resists the attack of Bombyx mori nucleopolyhedrovirus (BmNPV), we analyzed progression of infection by BmNPV in highly resistant silkworm NB?highly susceptible silkworm 306 and near isogenic line BC7. After orally administered with same dose of Wild-type BmNPV at 5th instar larvae,we detected copy numbers of virus in midgut, hemolymph and fat body at nine time points post infection by RealTime-PCR respectively. Nearly same copy numbers of virus were detected in midgut of three silkworm strains at 2hour post infection (hpi). Infection in hemolymph was widespread by 12hpi, by which time the fat body also showed infection. After 72hpi, copy numbers of BmNPV in three tissues of silkworm NB and BC7 remained in low level. In contrast, marked amplification of BmNPV (nearly 108-fold higher in the hemolymph than silkworm NB) was observed in three tissues of silkworm 306. These results indicate that suppressing replication of virus in vivo is the main mechanism by which highly resistant silkworm shows antiviral activity against BmNPV

关键词： Bombyx mori nucleopolyhedrovirus resistance RealTime-PCR

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初中校长对工作负荷的主观感受研究——以赣南初中校长为例

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新课程(中) 2014年第9期 142-143页

作者：张春兰胡可平赣州师范高等专科学校江西省赣南医学院

通过对赣南初中校长的工作压力调查,发现感觉"身心疲惫"的比例较高,同时发现,性别、年龄、教育程度、任职时间、职务、学校性质、学校地点、家庭的不同,影响到初中校长对工作的主观感受。

关键词：初中校长工作负荷主观感受

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工作坊在臀肌挛缩症患者康复护理中的应用

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中外医学研究 2019年第25期17卷 91-94页

作者：胡克萍陈惠雅董鸿雁佛山市中医院

目的:探讨工作坊在臀肌挛缩症(gluteal muscles contracture,GMC)患者康复护理中的应用效果。方法:将60例臀肌挛缩症患者随机分成观察组和对照组,各30例。对照组采用传统康复训练护理,观察组采用工作坊模式进行康复指导。观察两组患者... 详细信息

目的:探讨工作坊在臀肌挛缩症(gluteal muscles contracture,GMC)患者康复护理中的应用效果。方法:将60例臀肌挛缩症患者随机分成观察组和对照组,各30例。对照组采用传统康复训练护理,观察组采用工作坊模式进行康复指导。观察两组患者术后下床时间、住院时间、术后并发症、疗效及患者对工作坊康复训练满意度。结果:观察组和对照组所有患者均获得完整随访资料,观察组平均随访(19.6±3.4)个月,对照组平均随访(17.3±4.3)个月。观察组术后下床时间、住院时间明显优于对照组,差异均有统计学意义(P<0.05)。观察组对工作坊康复训练满意度明显优于对照组,差异有统计学意义(P<0.05)。观察组康复疗效的优良率高于对照组,差异有统计学意义(P<0.05)。观察组术中未发现重要的出血点,术后2例出现皮下血肿及皮下积液,经换药加压包扎后自愈,无神经血管损伤及感染等并发症,无复发。结论:臀肌挛缩症关节镜松解术后采用工作坊康复训练能缩短住院时间,改善关节功能。

关键词：臀肌挛缩症工作坊康复关节镜护理

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