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Journal of Energy Chemistry 2020年第4期29卷 47-51页

作者： Sangryun Kim kentaro harada Naoki Toyama Hiroyuki Oguchi Kazuaki Kisu Shin-ichi Orimo Institute for Materials Research Tohoku UniversitySendai 980-8577Japan WPI-Advanced Institute for Materials Research(WPI-AIMR) Tohoku UniversitySendai 980-8577Japan New Industry Creation Hatchery Center(NICHe) Tohoku UniversitySendai 980-8579Japan

We report on an all-solid-state battery that employs a closo-type complex hydride solid electrolyte and a LiCoO2 *** modification between the solid electrolyte and cathode with a LiNbO3 buffer layer enables reversible charge-discharge cycling with a cell voltage of 3.9V (***^+/Li) at room *** analyses clarify that the given modification effectively suppresses side reactions at the cathode/solid electrolyte *** interfacial resistance is lowered by ca.10 times with a 5 nm thick LiNbO3 buffer layer compared to that without a buffer layer,so that a discharge capacity of 109 mAh g^-1 is *** results suggest that interfacial modification can be a viable approach to the development of high-voltage all-solid-state batteries using closo-type complex hydride solid electrolytes and oxide cathodes.

关键词： All-solid-state batteries Closo-type complex hydride Solid electrolyte High-voltage cathodes Interfacial modification

New combination test for hepatitis C virus genotype and viral load determination using Amplicor GT HCV MONITOR test v2.0

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World Journal of Gastroenterology 2005年第4期11卷 469-475页

作者： Motokazu Mukaide Yasuhito Tanaka Hirokazu Kakuda Kei Fujiwara Fuat Kurbanov Eturo Orito kentaro Yoshioka Kiyotaka Fujise Shoji harada Takazumi Kozaki Kazuo Takemura Kazumasa Hikiji Masashi Mizokami SRL Inc. 5-6-50ShinmachiHino-shiTokyo 191-0002Japan Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences KawasumiMizuhoNagoya 467-8601Japan Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences KawasumiMizuhoNagoya 467-8601Japan Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences KawasumiMizuhoNagoya 467-8601 Japan Third Department of Internal Medicine Nagoya University School of Medicine65 TsurumaShowaNagoya 466-8550Japan Division of Gastroenterology and Hepatology Department of Internal Medicine (Kashiwa) The Jikei University School of Medicine163-1KashiwashitaKashiwaChiba 277-8567Japan SRL Inc. 5-6-50ShinmachiHmo-shiTokyo 191-0002Japan

AIM: To develop a new sensitive and inexpensive hepatitis C virus (HCV) combination test (HCV Guideline test) that enables the determination of HCV genotypes 1, 2 and 3, and simultaneous determination of HCV viral load using commercial Amplicor GT HOV MONITOR test v2.0 (microwell version). METHODS: The HCV Guideline test used the PCR product generated in commercial Amplicor GT HCV Monitor test v2.0 for viral load measurement using microwell plate version of Amplicor HCV Monitor and also captured on separate plates containing capture probes and competitive oligonucleotide probes specific for HCV genotypes 1, 2 and 3, The HCV genotype was subsequently determined using the biotin-labeled PCR product and five biotin-labeled HCV-specific probes. RESULTS: The sensitivity of the HCV Guideline test was 0.5 KIU/mL. Specificity of the HCV Guideline test was confirmed by direct sequencing of HCV core region and molecular evolutionary analyses based on a panel of 31 samples. The comparison of the HCV Guideline test and an in-house HCV core genotyping assay using 252 samples from chronic hepatitis C patients indicated concordant results for 97.2% of samples (59.5% genotype 1, 33.7% genotype 2, 6.0% genotype 3, and 0.8% mixed genotypes). Similarly, the HCV Guideline test showed concordance with a serological test, and the serological test failed to assign any serotype in 12.7% of the samples, indicating a better sensitivity of the HCV Guideline test. CONCLUSION: Clinically, both viral load and genotypes (1, 2 and 3) have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C based on guidelines and they are, in normal circumstances, performed as separate stand-alone assays. The HCV Guideline test is a useful method for screening large cohorts in a routine clinical setting for determining the treatment regimen and for predicting the outcome of antiviral therapy of chronic hepatitis C.

关键词： Hepatitis CVirus HCV Guideline test Viral Load Genotype

Change in reproductive and dispersal traits in the water strider, Aquarius paludum(Fabricius) and global warming

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Natural Science 2013年第1期5卷 156-162页

作者： Tetsuo harada Takashi Shiraki Shiho Takenaka Takero Sekimoto kentaro Emi Tomoya Furutani Laboratory of Environmental Physiology Graduate School of Integrated Arts and Sciences Kochi University Kochi Japan Laboratory of Environmental Physiology Graduate School of Integrated Arts and Sciences Kochi University Kochi Japan

This study aims to examine the following three hypotheses on the impact of global warming on the populations of the water strider, Aquarius paludum in the Kochi-Nankoku area (33。30'N) of Kochi prefecture, Japan through the recent data collected in 2009-2011. 1) Has the generation number increased? 2) Has aestivation appeared in adults? 3) Have overwintering adults stopped dispersing between the water surface and overwintering lands-sites far away from water and, instead, overwintered on/near the shore? Sampling data showed that the number of generations may have increased from three (1989-2002 strains) and four (2004-2008) to five (2009-2011) per year in Kochi (33。N). The ratio of adults having well developed flight muscles decreased from 45% in 1995 to 24% - 28% in 2009-2011 inoverwintering adults collected from the field in fall likely as a result of histolysis. “Mosaic-typed” wing morph group with long fore-wings and short hind-wings newly appeared in 2009-2011 in the Kochi-Nankoku overwintering populations. The mosaic-typed wings group cannot fly and the black and long fore wings might function as absorbing apparatus of sun-lights in the daytime of winter. Some overwintering adults seem to stop migrating between water bodies and overwintering sites on land far from the water bodies and overwinter, instead, near the shore. The use of Aquarius paludum as a biological indicator would be possible in the future, because this species can respond and change their reproductive and dispersal characteristics to the global change.

关键词： Adult Diapause Aestivation Dispersal Long Fore-Wing and Short Hind-Wing

Clinical Utility of Procalcitonin for Differentiating between Cryptogenic Organizing Pneumonia and Community-Acquired Pneumonia

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International Journal of Clinical Medicine 2015年第6期6卷 372-376页

作者： Satoshi Takeda Nobuhiko Nagata Hiroyuki Miyazaki Takanori Akagi Taishi harada Masaru Kodama Shinichiro Ushijima Takashi Aoyama kentaro Wakamatsu Masaki Fujita kentaro Watanabe Department of Respiratory Medicine Fukuoka University Chikushi Hospital Chikushino Japan Department of Respiratory Medicine National Hospital Organization Omuta Hospital Omuta Japan Department of Respiratory Medicine Faculty of Medicine Fukuoka University Fukuoka Japan

Background: Differentiating cryptogenic organizing pneumonia (COP) from community-acquired pneumonia (CAP) can be difficult in some cases. Objective: To clarify the clinical utility of procalcitonin (PCT) levels for differentiating between COP and CAP. Methods: Blood PCT levels, leukocyte count, C-reactive protein concentration, number of segments involved on computed tomography (CT) images, and pneumonia severity assessment scale were retrospectively investigated from clinical charts and chest CT images of COP and CAP patients who were admitted to our hospital from 2012 to 2014. The clinical characteristics of COP patients were compared to those of CAP patients for whom causative organisms were not detected. Results: There were 16 COP and 94 CAP patients for whom causative organisms were not detected. Blood PCT levels of all COP patients were less than 0.16 ng/dL, and significantly lower than those of CAP patients (p = 0.0004), while the number of involved segments was significantly higher than that of CAP patients (p = 0.0001). Blood PCT levels and the number of involved segments remained independently significant for differentiating between COP and CAP by multivariate analysis. Receiver operating characteristics curve analysis revealed that 7 was the best cut-off number for involved segments to differentiate between COP and CAP patients with low PCT levels (sensitivity 85.7%, specificity 94.7%). Conclusion: A combination of PCT levels and number of involved segments on CT images is useful for differentiation between COP and CAP.

关键词： Cryptogenic Organizing Pneumonia Procalcitonin Community-Acquired Pneumonia Computed Tomography

Comparative Study of Salinity Tolerance an Oceanic Sea Skater, Halobatesmicansand Its Closely Related Fresh Water Species, Metrocorishistrio

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Natural Science 2014年第14期6卷 1141-1148页

作者： Takero Sekimoto Yuki Osumi Takashi Shiraki Akane Kobayashi kentaro Emi Mitsuru Nakajo Masatoshi Moku Vladimir Kostal Chihiro Katagiri Tetsuo harada Laboratory of Environmental Physiology Graduate School of Integrated Arts and Sciences Kochi University Kochi Japan Atmosphere and Ocean Research Institute The University of Tokyo Tokyo Japan Institute of Entomology Biology Center of the Academy of Sciences CR Ceske Budejovice Czech Republic Division of Life Science and Engineering School of Science and Engineering Tokyo Denki University Hatoyama-cho Japan

This study aims, first, to examine the limit for tolerance to lower salinity by an oceanic sea skater, Halobates micans, and , second, to make it clear whether exclusively fresh water Halobatinae species, Metrocoris histrio has salinity tolerance. Adults of H. micans were collected using Neuston Net from the starboard side of R/V MIRAI on a fixed station at 8°S, 80°E, whereas those of M. histrio were collected from a small pond filled with a spring fresh water in Kochi (33°N, 133°E), Japan. Time in survival was measured in starved condition under several salinity conditions: 0‰, 2‰, 4‰, 6‰, 8‰, 9‰ and 10‰ for H. micans;0‰, 5‰, 10‰, 12.5‰, 15‰ for M. histrio. Half of adults were in coma due to lower salinity under 10‰ and time in survival was less than 10 hours under less than 4‰ for H. micans. Time in survival was half at 5‰ of 80 hours on average at 0‰ as a control and less than 10 hours at 10‰ or higher salinity for M. histrio. Relatively flexible osmo-regulation ability by H. micans would be related to wide variety of salinity condition of surface oceanic water, whereas very limited tolerance even to lower salinity of 5‰ may be permitted by the no chances to be exposed to brackish water in natural habitats of M. histrio. This study showed that salinity tolerance of Halobatinae species would reflect, directly, the salinity condition of their habitats.

关键词： Oceanic Sea Skaters Fresh Water Halobates mican sand Its Closely Related Fresh Water Species Metrocoris histrio

Comparison of temperature preference for habitat among three species of oceanic sea skaters,Halobates micans,*** and ***

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Natural Science 2013年第12期5卷 9-15页

作者： Mitsuru Nakajo Takero Sekimoto kentaro Emi Ryuta Ide Kai Wada Takao Inoue Masatoshi Moku Vladimír Kostál Chihiro Katagiri Tetsuo harada Laboratory of Environmental Physiology Graduate School of Integrated Arts and SciencesKochi UniversityKochiJapan Atmosphere and Ocean Research Institute The University of TokyoTokyoJapan Institute of Entomology Biology Center of the Academy of Sciences CRCeske BudejoviceCzech Republic Division of Life Science and Engineering School of Science and EngineeringTokyo Denki UniversityTokyoJapan

The main purpose of this study is to investigate temperature preference for habitat of three species of oceanic sea skaters, Halobates micans, H. germanus and H. sericeus. For each of the three species, we examined the relationship between population density and surface water and air temperatures in the sampling sites during three science cruises in the tropical to temperate zone of the Pacific Ocean. A higher density of H. micans was observed in sites with higher water and air temperatures at 28℃?- 30℃?than that at site with lower temperatures of 23℃?- 28℃. A higher density of H. germanus was observed in sites with extremely high temperatures of 29.6℃?and 30℃?than that in sites with moderately high temperatures of 28℃?- 29℃?in the Pacific Ocean, while a relatively high density was observed in sites with a wide range of temperatures from 24℃?- 30℃?in the southern hemisphere Pacific Ocean. The relatively low temperature preference of 23℃?- 28℃?exhibited by H. sericeus may be related to its size as the smallest among the three species and its relatively wide latitudinal distribution ranging up to 40?N.

关键词： Oceanic Sea Skaters Temperature Preference Latitudinal Distribution Pacific Ocean