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Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

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Journal of Animal Science and Biotechnology 2020年第1期11卷 44-57页

作者： MariaPortia B.Nagata Junki Egashira Naoto Katafuchi Kenji Endo Kazuko Ogata Kenichi yamanaka Tadayuki Yamanouchi Hideo Matsuda Yutaka Hashiyada Kenichi Yamashita Advanced Manufacturing Research Institute National Institute of Advanced Industrial Science and Technology(AIST)807-1 Shuku-machiTosuSaga 841-0052Japan Saga Prefectural Livestock Experiment Station 23242-2 Yamauchi-machiMiyanoTakeoSaga 849-2305Japan Morinaga Dairy Service Co.Ltd. 1-159 ToyoharaotsuNasu-gun Nasu-machiTochigi 329-3224Japan National Livestock Breeding Center(NLBC) 1 OdakuraharaOdakuraNishigo-muraNishishirakawa-gunFukushima 961-8511Japan Institute of Livestock and Grassland Science National Agriculture and Food Research Organization(NARO)Ikenodai 2TsukubaIbaraki 305-0901Japan Faculty of Agriculture Saga University1 Honjo-machiSaga 840-8502Japan.7Ishikawa Prefectural University1-308 SuematsuNonoichi-shiIshikawa 921-8836Japan.

Background:The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal ***,a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation,rendering the frozen-thawed semen impractical for routine *** is known to reduce sperm lifespan and *** in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination(AI)*** goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation ***:Our strategy of sperm selection based on rheotaxis and thermotaxis(SSRT)on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity,viability and biological function related to fertilization *** strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat *** technique protected further sublethal damage to bovine spermatozoa(higher%cryosurvival than the control)and resulted in the improvement of DNA *** selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo ***:These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival,longevity of post-thawed sperm,and optimization of in vivo fertilization,embryo development and calving as supported by the favorable results of field fertility study.

关键词： Cryopreservation Fertility Livestock Rheotaxis Spermatozoa Thermotaxis

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Vacuum Insulation and Achievement of 980 keV,185 A/m^2 H-Ion Beam Acceleration at JAEA for the ITER Neutral Beam Injector

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Plasma Science and Technology 2013年第2期15卷 179-183页

作者： Hiroyuki TOBARI Masaki TANIGUCHI Mieko KASHIWAGI Masayuki DAIRAKU Naotaka UMEDA Haruhiko yamanaka Kazuki TSUCHIDA Jumpei TAKEMOTO Kazuhiro WATANABE Takashi INOUE Keishi SAKAMOTO Japan Atomic Energy Agency (JAEA) Mukouyama 801-1 Naka Ibaraki 311-1093 Japan

Abstract Vacuum insulation of -1 MV is a common issue for the HV bushing and the accel- erator for the ITER neutral beam injector （NBI）. The HV bushing as an insulating feedthrough has a five-stage structure and each stage consists of double-layered insulators. To sustain -1 MV in vacuum, reduction of electric field at several triple points existing around the double-layered insulators is a critical issue. To reduce electric field simultaneously at these points, three types of stress ring have been developed. In a voltage holding test of a full-scale mockup equipped with these stress rings, 120% of rated voltage was sustained and the voltage holding capability required in ITER was verified. In the MeV accelerator, whose target is the acceleration of a H- ion beam of 1 MeV, 200 A/mS, the gap between the grid support was extended to suppress breakdowns triggered by electric field concentration at the edge and corner of the grid support. This modi- fication improved the voltage holding capability in vacuum, and the MeV accelerator succeeded in sustaining -1 MV stably, b-~rthermore, it appeared that the H- ions beam was deflected and a part of the beam was intercepted at the acceleration grid, This causes high heat load on the grids and breakdowns during beam acceleration. To suppress the direct interception, a new grid was designed with proper aperture displacement based on a three dimensional beam trajectory analysis, As a result, 980 keV, 185 A/ms H- ion beam acceleration has been demonstrated, which is close to the ITER requirement.

关键词： neutral beam injector (NBI) negative ion beam HV bushing vacuum insu-lation

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使用配备单纳米颗粒应用模块的Agilent 7900 ICP-MS实现单个纳米颗粒的自动化高灵敏度分析

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环境化学 2016年第4期35卷 833-835页

作者： Michiko yamanaka Kazuo yamanaka Takayuki Itagaki Steven Wilbur Ed McCurdy 安捷伦科技公司日本安捷伦科技公司美国安捷伦科技公司英国

使用配备ICP-MS Mass Hunter软件专用单纳米颗粒应用模块的Agilent 7900 ICP-MS成功实现了纳米颗粒的测定和表征.集成的数据分析软件能够快速自动化地执行单颗粒分析所需的复杂计算,同时能够在单颗粒模式下对结果进行简单、可视化的优... 详细信息

使用配备ICP-MS Mass Hunter软件专用单纳米颗粒应用模块的Agilent 7900 ICP-MS成功实现了纳米颗粒的测定和表征.集成的数据分析软件能够快速自动化地执行单颗粒分析所需的复杂计算,同时能够在单颗粒模式下对结果进行简单、可视化的优化与验证.sp-ICP-MS方法可同时提供粒径分布与样品浓度信息,这是其他技术通常无法达到的.

关键词：纳米颗粒自动化分析

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Primary biliary cirrhosis after aortoiliac reconstruction surgery using a Y-graft: A case report

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World Journal of Gastroenterology 2005年第39期11卷 6219-6220页

作者： Tomoko Inoue Katsuya Shiraki Hiroyuki Fuke Yutaka yamanaka Kazumi Miyashita Keiichi Ito Masahiro Suzuki Kazushi Sugimoto Kazumoto Murata Takeshi Nakano First Department of Internal Medicine Mie University School of Medicine 2-174 EdobashiTsuMie 514-8507Japan

Primary biliary cirrhosis (PBC) is an autoimmune disease characterized by anti-mitochondrial antibodies and destruction of intra-hepatic bile ducts. Though little is known about the etiology of PBC, some reports suggest that xenobiotics and viral infections may induce PBC. We describe a case of PBC after the aortoiliac reconstruction surgery using a Y-graft.

关键词： Gall bladder cancer Metastasis Metastatic cardiac tumor

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The active role of glial cells in familial and sporadic motor neuron disease

The active role of glial cells in familial and sporadic moto...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Koji yamanaka RIKEN Brain Science Institute

＜正＞Dominant mutations in the Cu/Zn superoxide dismutase（SOD1） gene lead to a familial form of amyotrophic lateral sclerosis（ALS）,an adult motor neuron *** ubiquitous expression of mutant SOD1 provokes progressive,selective motor neuron degeneration in human and rodents due to an acquired toxic property（ies） of the mutant,the distinct roles of mutant toxicity within motor neurons and non-neuronal cells are recently established by our cell-type specific gene ablation from mutant SOD1 *** toxicity（ies） within astrocytes and microglia accelerates disease progression,indicating that glial cells contribute to non-cell autonomous neurodegeneration. Recent discovery of TDP-43 protein accumulation as a pathological hallmark of sporadic ALS prompted us to test whether SOD1-linked familial ALS and sporadic ALS share the common disease mechanisms in glial *** address this question,we have compared gene expression profile of spinal cords of SOD1 mice and sporadic ALS patients by establishing the cell-type specific *** overlap of misregulated genes enriched in microglia was observed in sporadic ALS and SOD1 mouse *** pathway analysis indicated the innate immune system,NF- kappaB signaling,and caspase-interleukin cascade that were misregulated in glial cells,suggesting these pathways may be the potential therapeutic targets of ALS.

关键词： glia motor neuron disease ALS SODl

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Medial tibial plateau morphology and stress fracture location:A magnetic resonance imaging study

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World Journal of Orthopedics 2017年第6期8卷 484-490页

作者： Kiminori Yukata Issei yamanaka Yuzuru Ueda Sho Nakai Hiroyoshi Ogasa Yosuke Oishi Jun-ichi Hamawaki Department of Orthopedic Surgery Yamaguchi University Graduate School of Medicine

AIM To determine the location of medial tibial plateau stress fractures and its relationship with tibial plateau morphology using magnetic resonance imaging(MRI).METHODS A retrospective review of patients with a diagnosis of stress fracture of the medial tibial plateau was performed for a 5-year period. Fourteen patients [three female and 11 male, with an average age of 36.4 years(range, 15-50 years)], who underwent knee MRI, were included. The appearance of the tibial plateau stress fracture and the geometry of the tibial plateau were reviewed and measured on *** Thirteen of 14 stress fractures were linear, and one of them stellated on MRI images. The location of fractures was classified into three types. Three fractures were located anteromedially(AM type), six posteromedially(PM type), and five posteriorly(P type) at the medial tibial plateau. In addition, tibial posterior slope at the medial tibial plateau tended to be larger when the fracture was located more posteriorly on *** We found that MRI showed three different localizations of medial tibial plateau stress fractures, which were associated with tibial posterior slope at the medial tibial plateau.

关键词： Magnetic resonance imaging Runner Stress fracture Tibial plateau Tibial posterior slope

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诱导多能干细胞(iPSCs):过去、现在和未来

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中国细胞生物学学报 2012年第10期34卷 1055-1062页

作者： Shinya yamanaka 朱丽华单位不详

诱导多能干细胞技术诞生至今已有6年多历史了,许多人认为这是生命科学和再生医学研究领域的一场革命,也是细胞生物学发展的里程碑,很多人预言这项技术迟早会获得诺贝尔生理医学奖。但是,对这项技术诞生的历史背景、发展历程、研究现状... 详细信息

诱导多能干细胞技术诞生至今已有6年多历史了,许多人认为这是生命科学和再生医学研究领域的一场革命,也是细胞生物学发展的里程碑,很多人预言这项技术迟早会获得诺贝尔生理医学奖。但是,对这项技术诞生的历史背景、发展历程、研究现状以及未来前景,能够真正了解的并不多。为此,我们将此项技术发明人日本京都大学iPS细胞研究和应用中心山中伸弥(Shinya yamanaka)最近发表的评述(yamanaka *** pluripotent stem cells:Past,present,and *** Stem Cell2012;10(6):678-84)翻译成中文以飨读者。

关键词：多能干细胞诱导干细胞技术诺贝尔生理医学奖日本京都大学细胞生物学再生医学生命科学

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Bacterial Infection in the Limbs of Patients with Rheumatoid Arthritis during Biological Agent Therapy

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Open Journal of Rheumatology and Autoimmune Diseases 2012年第3期2卷 47-52页

作者： Hajime yamanaka Kenichiro Goto Munetaka Suzuki Department of Orthopaedic Surgery National Hospital Organization Shimoshizu Hospital Yotsukaido City Japan

Biological therapies in rheumatoid arthritis (RA) are known to increase the risk of serious infections. The present study was performed to evaluate the clinical features of bacterial infections occurring in the limbs during biological therapies in patients with RA. By March 2011, 11 RA patients (14 limbs) treated with biological agents at our institution required hospitalization due to bacterial infections occurring in the limbs. These patients had an average age of 53.7 years old. Infections occurred an average of 19 months after biological treatment. Two limbs in one patient were treated with infliximab, eight limbs in six patients were treated with etanercept, one limb in one patient was treated with adalimumab, and three limbs in three patients were treated with tocilizumab. Cellulitis occurred in 7 limbs, late infections after total knee arthroplasty occurred in two limbs, early infections after orthopedic surgery occurred in three limbs, and septic arthritis occurred in two limbs. Four cases had comorbidities—liver cirrhosis and diabetes mellitus in one and three cases, respectively. All patients were treated using corticosteroid with an average dose of 4.6 mg daily. Seven limbs required surgical treatment. All patients finally recovered. Ten limbs continued treatment with biological agents. Care must be taken regarding bacterial infection in the limbs of RA patients treated by using biological agents, particularly those with comorbidities. Further studies are required to confirm means of preventing such infections in daily practice.

关键词： Bacterial Infection Limbs Rheumatoid Arthritis Biological Agents

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Cardiac metastases of gallbladder carcinoma

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World Journal of Gastroenterology 2005年第13期11卷 2048-2049页

作者： Tomoko Inoue Katsuya Shiraki Hiroyuki Fuke Yutaka yamanaka Kazumi Miyashita Keiichi Ito Masahiro Suzuki Kazushi Sugimoto Kazumoto Murata Takeshi Nakano First Department of Internal Medicine

This report describes the case of a 68-year-old woman diagnosed with advanced gallbladder cancer, whose autopsy revealed multiple metastases, including cardiac metastases.

关键词： Gallbladder cancer Metastasis Metastatic cardiac tumor

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客车的现行安全措施（一）

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移动电源与车辆 1992年第1期23卷 38-44页

作者： A.yamanaka 姚永昌

只有当人，车辆及其环境，各方面相协调时，才有可能保证汽车安全，自从汽车发明以来，以车辆的行驶特性，耐久性和经济性方面已经做了许多改进，但是，运输环境的研究，尤其是现行安全措施的改进，直到1960年才开始。本文所讨论的最重... 详细信息

只有当人，车辆及其环境，各方面相协调时，才有可能保证汽车安全，自从汽车发明以来，以车辆的行驶特性，耐久性和经济性方面已经做了许多改进，但是，运输环境的研究，尤其是现行安全措施的改进，直到1960年才开始。本文所讨论的最重要问题，就是司机的视野，车辆的稳定性和可操作性，并谈到期望的传动辅助装置在不久将来的发展。

关键词：客车驾驶视野安全

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