检索结果-南通市图书馆

基础医学与临床 2011年第12期31卷 1309-1313页

作者：雷健王广李艳常青 kenneth KH lee John YH Chan 杨雪松暨南大学医学院组织与胚胎学教研室再生医学教育部重点实验室广东广州510632 香港中文大学解剖系

目的探讨BRE基因对鸡胚胎早期体节发育的作用。方法显微注射法将含有BRE基因的质粒注射入HH10期鸡胚神经管,活体胚胎细胞电穿孔方法转染半侧神经管,以另一侧为正常对照侧,原位杂交及免疫荧光法观察过表达BRE基因后体节的发育。结果 BRE... 详细信息

目的探讨BRE基因对鸡胚胎早期体节发育的作用。方法显微注射法将含有BRE基因的质粒注射入HH10期鸡胚神经管,活体胚胎细胞电穿孔方法转染半侧神经管,以另一侧为正常对照侧,原位杂交及免疫荧光法观察过表达BRE基因后体节的发育。结果 BRE基因在鸡胚表达始于神经板,而后高表达在脑泡、神经管及体节。过表达BRE基因后,神经管形态及PAX7和FGF8表达与对照侧相比无明显异常,而实验侧体节PAX7标记发现生皮肌节较大,FGF8表达与对照侧相比无明显异常。结论过表达BRE基因对体节的形成和分化有促进作用,其机制可能与PAX7相关。

关键词：神经管体节 BRE PAX7 FGF8

维普期刊数据库

Gastrointestinal Kaposi's sarcoma: Case report and review of the literature

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pharmacology and Therapeutics 2015年第3期6卷 89-95页

作者： Ann Joo lee Lacie Brenner Bashar Mourad Carmela Monteiro kenneth J Vega Juan Carlos Munoz Division of Gastroenterology Department of Medicine University of Florida College of Medicine Jacksonville Department of Medicine University of Florida College of Medicine Jacksonville Department of Pathology University of Florida College of Medicine Jacksonville Division of Digestive Diseases and Nutrition University of Oklahoma Health Sciences Center

Kaposi's sarcoma(KS) of the gastrointestinal tract is not an uncommon disease among individuals with acquired immunodeficiency syndrome(AIDS). The majority is asymptomatic, and for this reason, gastrointestinal KS(GI-KS) remains undiagnosed. With continued tumor growth, considerable variation in clinical presentation occurs including abdominal pain, nausea, vomiting, iron deficiency anemia(either chronic or frank gastrointestinal bleeding), and rarely mechanical obstruction alone or combined with bowel perforation. Endoscopy with biopsy allows for histological and immunohistochemical testing to confirm the diagnosis of GI-KS among those with clinical symptoms. In previous studies, dual treatment with highly active antiretroviral therapy and systemic chemotherapy have been associated with improved morbidity and mortality in individuals with visceral KS. Therefore, investigators have suggested performing screening endoscopies in select patients for early detection and treatment to improve outcome. In this review, we describe a 44 years old man with AIDS and cutaneous KS who presented for evaluation of postprandial abdominal pain, vomiting, and weight loss. On upper endoscopy, an extensive, infiltrative,circumferential, reddish mass involving the entire body and antrum of the stomach was seen. Histologic examination later revealed spindle cell proliferation, and confirmatory immunohistochemical testing revealed human herpes virus 8 latent nuclear antigen expression consistent with a diagnosis of gastric KS. Following this, we present a comprehensive review of literature on KS with emphasis on gastrointestinal tract involvement and management.

关键词： Kaposi sarcoma Acquired immunodeficiency syndrome Gastrointestinal endoscopy Epidemiology Gastrointestinal tumor symptoms

Endoscopic ultrasound-through-the-needle biopsy in pancreatic cystic lesions: A large single center experience

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Endoscopy 2019年第11期11卷 531-540页

作者： Rintaro Hashimoto John G lee kenneth J Chang Nabil El Hage Chehade Jason B Samarasena H.H.Chao Comprehensive Digestive Disease Center Division of Gastroenterology and Hepatology Department of Medicine University of CaliforniaIrvine

BACKGROUND Establishing a diagnosis of pancreatic cystic lesions(PCLs)preoperatively still remains ***,endoscopic ultrasound(EUS)-through-the-needle biopsy(EUS-TTNB)using microforceps in PCLs has been made *** To assess the efficacy and safety of EUS-TTNB in the diagnosis of *** We retrospectively collected data of patients with PCLs who underwent both EUS-fine-needle aspiration(FNA)for cytology and EUS-TTNB at our institution since ***-FNA for cytology was followed by EUS-TTNB in the same *** of the cyst location,primary diagnosis,adverse events,and comparison between the cytologic fluid analyses and histopathology was *** success of EUS-TTNB was defined as visible tissue present after *** success was defined as the presence of a specimen adequate to make a histologic or cytologic *** A total of 56 patients(mean age 66.9±11.7,53.6%females)with PCLs were enrolled over the study *** mean cyst size was 28.8 mm(12-85 mm).The EUS-TTNB procedure was technically successful in all patients(100%).The clinical success rate using EUS-TTNB was much higher than standard EUS-FNA,respectively 80.4%(45/56)vs 25%(14/56).Adverse events occurred in 2 patients(3.6%)who developed mild pancreatitis that resolved with medical *** TTNB specimens,23 of 32 cases(71.9%)with intraductal papillary mucinous neoplasm were further differentiated into gastric type(19 patients)and pancreaticobiliary type(4 patients)based on immunochemical *** EUS-TTNB for PCLs was technically feasible and had a favorable safety ***,the diagnostic yield for PCLs was much higher with EUS-TTNB than standard EUS-FNA cytology and fluid carcinoembryonic *** should be considered as an adjunct to EUS-FNA and cytologic analysis in the diagnosis and management of PCLs.

关键词： Pancreatic cyst lesion Endoscopic ultrasound Endoscopic ultrasound-guided fine needle aspiration Cyst fluid Biopsy

Factors Affecting Mesenchymal Stromal Cells Yield from Bone Marrow Aspiration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2011年第1期23卷 43-48页

作者： Jing Li Wilfred Hing-Sang Wong Shing Chan James Chor-San Chim kenneth Man-Chee Cheung Tsz-Leung lee Wing-Yan Au Shau-Yin Ha Albert Kwok-Wei Lie Yu-Lung Lau Raymond Hin-Suen Liang Godfrey Chi-Fung Chan China Oxford Centre for International Health Research Cardiovascular Institute ＆ Fuwai HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical CollegeBeijing 100037China Department of Paediatrics and Adolescent Medicine Department of Medicine Department of Orthopaedics ＆Traumatology the University of Hong KongHKSARChina

Objective：This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells（MSC）.Methods：We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from：（1） donors of different ages;（2） healthy donors and patients with leukemia;（3） bone marrow aspirated at different time points during marrow harvesting;（4） bone marrow harvested by different ***：During the process of harvesting,the number of MSC significantly decreased with increase number of aspiration,from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated,and 50/ml after 200 ml bone marrow *** number of MSC retrieved from leukemia patients（99/ml bone marrow） was significantly lower than that of healthy donors（708/ml bone marrow）.However,there was no significant difference in growth *** was no significant age-related difference of MSC yielded from donors 55 *** there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole ***：The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting *** marrow from donors 55 years was equally good as MSC *** autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.

关键词： Mesenchymal stromal cell CFU-F Bone marrow aspiration Leukemia

维普期刊数据库

SMTA华东高科技技术研讨会论文沉镍层的高磷含量是否引致黑镍的主要原因?

在线全文

学校读者我要写书评

暂无评论

SMTA华东高科技技术研讨会论文沉镍层的高磷含量是否引致黑镍的...

The Optical and Two-Photon Properties of Amylose-Encapsulated Chromophore

2014中国高端SMT学术会议

作者： Dipl.-Ing.Mustafa ?zk?k Joe McGurran kenneth lee Atotech Deutschland GmbH Atotech USA Inc.Rock Hill SCUSA Atotech Asia Pacific Ltd.

在电子世界中,"黑镍"的现象被认为是线路板中的沉镍浸金工艺的一种失效模式。许多技术文献都有描述及讨论此失效的机理和根原,但亦有些是支持黑镍失效的传统"神话"。可是这些关于黑镍的论文或文章根本没有提及此问题的真正本质。对关于... 详细信息

在电子世界中,"黑镍"的现象被认为是线路板中的沉镍浸金工艺的一种失效模式。许多技术文献都有描述及讨论此失效的机理和根原,但亦有些是支持黑镍失效的传统"神话"。可是这些关于黑镍的论文或文章根本没有提及此问题的真正本质。对关于指高磷含量是引致黑镍的根原（没有有关的化学机理或实质的量度数据支持）但没有足够的科学根据所支持。这种猜测乃基于过往被广泛流传的不确定性黑镍失效的原因,导致很多线路板制作在规格上限制了沉镍层的最高磷含量以减少这种缺陷发生的可能性。此文章主要是要探讨这问题:"沉镍层的磷含量是否引致黑镍的主要原因?’此问题我们会从纯粹科学的方法配合相关的技术数据如微切片结果、焊点可靠性测试及其相应的磷含量去进行分析及探讨。本文章将会介绍并解释黑镍失效的机理,及与另一种类似的失效模式-"脆性破裂"作为比较。此文还会提供一些分析实例,如不同的磷含量对化学沉镍面的腐蚀程度对比,与及在高温条件变化下焊点的可靠性测试表现作详细报告。

关键词：黑鎳沉镍浸金高磷低磷中磷

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Chemistry and Chemical Engineering 2014年第2期8卷 199-203页

作者： David Sukwon Chung James kenneth Park Judy Sulki lee Choong Sung lee Ester Yang Moon-Jun Brian Kim Sung-Jae Chung Division of Biology and Medicine Brown University Providence 02912 USA Jefferson High School Shenandoah Junction WV25442 USA McDonogh School Owings Mills MD 21117 USA Glenelg Country School Ellicott City MD 21042 USA Mount Holyoke College South Hadley MA 01075 USA Department of Biological and Environmental Engineering Cornell University NY 14850 USA Chemistry School of Arts and Sciences Marymount University VA 22207 USA

Encapsulation of DASP (4-[4-(dimethylamino)styryl]-1-octylpyridinium bromide) with amylose, and conformational transitions of amylose upon complexation with the dyes were investigated using UV-vis, fluorescence and two-photon absorption properties in various Dimethyl Sulfoxide ( DMSO)/H2O mixtures. Encapsulation of the dye was achieved by dilution of DMSO stock solutions of the chromophores (1 × 10-3 M) and amylose (1 × 10-1 M) with DMSO, followed by a dropwise addition of H2O with continuous stirring at room temperature. At less than 40% aqueous DMSO solutions, the chromophores had a stable encapsulation with amylose and showed over 100 times the fluorescence intensity of non-encapsulated chromophores. Both of the dye solution with and without amylose also exhibit 2PA (two-photon absorption), as determined by nonlinear absorption measurements using 10 ns laser pulses at 800 nm. The respective values of two-photon cross-sections are 13.3 × 10-20 for encapsulated dye solution and 2.53 × 10-20 cm4/GW for non encapsulated dye solution. The data clearly indicate that the contribution of the encapsulated dye dominates the two-photon absorption cross-section of the non encapsulated dye at 800 nm.

关键词：双光子吸收直链淀粉发色团包封染料溶液络合染料 DMSO 性质

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国启动月岩取样返回的探月任务

英语文摘 2021年第2期 58-63页

作者： Steven lee Myers kenneth Chang 何金娥

It has been four decades since lunar samples;were brought to Earth,and the Chang’e-5;spacecraft’s;bounty;could have great scientific value.上一次人类采集月球样本送回地球还是40年以前,嫦娥五号探测器此举具有重大科研价值。

关键词：

社区步行通达性对独栋住宅房价的效益评估--美国德克萨斯州奥斯丁市空间特征分析

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

城市交通 2016年第4期14卷 75-91页

作者：李威 kenneth Joh Chanam lee Jun-Hyun Kim Han Park Ayoung Woo 张斯阳(译) 德州农工大学建筑学院景观建筑和城市规划系美国德克萨斯州大学城77843-3137 德州交通研究所美国德克萨斯州大学城77843-3135 中国城市规划设计研究院北京100037

通过分析美国德克萨斯州奥斯丁市2010—2012年独栋住宅的销售价格,以街道智慧步行指数(Street Smart Walk Score)和人行道密度(Sidewalk Density)作为衡量指标,研究社区步行通达性对房价的影响。使用Cliff-Ord空间特征模型(即广义空间模... 详细信息

通过分析美国德克萨斯州奥斯丁市2010—2012年独栋住宅的销售价格,以街道智慧步行指数(Street Smart Walk Score)和人行道密度(Sidewalk Density)作为衡量指标,研究社区步行通达性对房价的影响。使用Cliff-Ord空间特征模型(即广义空间模型,General Spatial Model,或者SAC)控制空间自相关影响。结果表明:在依赖小汽车的社区通过增加设施可达性来提升步行通达性的举措并不能提高房价;增加人行道也只能最小限度地提高房价。投资社区便利设施和人行道对适宜步行社区的房价提升效果比依赖小汽车的社区显著。

关键词： Cliff-Ord空间特征模型广义空间模型(SAC) 房价步行指数适宜步行社区步行通达性空间自相关空间特征模型

维普期刊数据库

在线全文

学校读者我要写书评

暂无评论

L波段雷达的25千瓦固态发射机

现代雷达 1981年第1期 151-156页

作者： kenneth J.lee 于鸿希

采用了40个高功率组件并联作为输出级的25千瓦L波段的固态雷达发射机已经研制出来了,对在这样功率电平下保持组件间的稳定和有效的功率合成的高功率技术进行了介绍,对发射机的整个设计方法和最终特性进行了讨论。

关键词：功率合成器组件输出端发射机电子设备功率电平雷达传感器波段