检索结果-南通市图书馆

Americ.n Journal of Analytic.l c.emistry 2011年第7期2卷 849-856页

作者： kenneth c. Madu P. O. Ukoha A. A. Attama 不详

A spec.rophotometric.method for the assay of lamivudine in pure form and in dosage form was developed in this study. The method was based on c.arge-transfer c.mplex formation between the drug, whic. ac.ed as n-donor while c.loranilic.ac.d and 2,3-dic.loro-5,6-dic.ano-1,4-benzoquinone (DDQ) ac.ed as a p-ac.eptor in a non-aqueous solvent in eac. c.se. c.loranilic.ac.d was found to form a c.arge-transfer c.mplex in a 1:1 stoic.iometry with lamuvudine (lamivudine-c.loranilic.ac.d) with a maximium absorption band at 521 nm. Also, DDQ was found to form a c.arge-transfer c.mplex in a 1:1 stoic.iometry with lamivudine (lamivudine-DDQ) with a maximium absorption band at 530 nm. The pH was obeyed at ac.d range. The c.mplexes obeyed Beer’s law at a c.nc.ntration range of 0.04 - 0.28 mg/ml. The thermodynamic.parameters c.lc.lated at different temperatures inc.uded the molar absorptivity, assoc.ation c.nstant, free energy c.ange, enthalpy and entropy. The proposed method has been c.nveniently applied in the analysis of c.mmerc.ally available lamivudine tablet with good ac.urac. and prec.sion.

关键词： c.arge-Transfer c.mplexation c.loranilic Ac.d DDQ Lamivudine Spec.rophotometric Determination Thermodynamic Studies pH Pharmac.utic.l Formulation

c.myc.K-ras对小鼠卵巢上皮细胞体外转化和体内致瘤的研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华肿瘤杂志 2006年第12期28卷 881-885页

作者：姚德生李力 kenneth Garson Barbara c.Vanderhyden 广西医科大学肿瘤医院妇瘤科南宁530021 加拿大渥太华大学癌症中心

目的探讨癌基因c.myc.K-ras在卵巢癌发生发展中的作用。方法用逆转录病毒转基因系统,将正常的c.myc.因和突变的K-ras基因先后导入小鼠卵巢上皮(MOSE)细胞,建立表达c.myc.K-ras基因的细胞株,分别称为MOSE-Myc.胞、MOSE-Ras细胞和MOSE... 详细信息

目的探讨癌基因c.myc.K-ras在卵巢癌发生发展中的作用。方法用逆转录病毒转基因系统,将正常的c.myc.因和突变的K-ras基因先后导入小鼠卵巢上皮(MOSE)细胞,建立表达c.myc.K-ras基因的细胞株,分别称为MOSE-Myc.胞、MOSE-Ras细胞和MOSE-RM细胞。通过细胞增殖实验、克隆形成实验、体外侵袭实验以及体内成瘤实验,研究转基因后MOSE细胞生物学特性的改变及恶性转化。结果c.myc.K-ras基因容易被重组逆转录病毒导入MOSE细胞,转导后靶细胞内分别有相应的c.myc.K-ras mRNA转录,以及相应的c.myc.62 000)和K-ras(21 000)蛋白的表达。MOSE-Ras组和MOSE-RM(MOSE-Ras／Myc.组细胞增殖能力显著强于MOSE和MOSE-Myc.(Pc.(Pc.不能形成克隆集落。MOSE-Ras组和MOSE-RM组能够穿透Matrigel,具有侵袭能力。MOSE-Ras组和MOSE-RM组细胞在裸鼠体内形成肿瘤,且肿瘤组织细胞仍然可见有K-ras和c.myc.白的表达;而MOSE组和MOSE-Myc.无肿瘤形成。结论重组逆转录病毒载体作为转基因的工具,转导效率高,可稳定表达,可以感染正常的细胞;突变的K-ras可使MOSE发生恶性转化,在体内形成肿瘤;c.myc.为一个核转录调节因子,不能使MOSE发生恶性转化,但可协同其他因子,加强其他因子的功能。

关键词：逆转录病毒载体卵巢肿瘤 K-ras c.myc 基因转移移植瘤模型

A mec.anistic.study of c.profloxac.n adsorption by goethite in the presenc. of silver and titanium dioxide nanopartic.es

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sc.enc.s 2022年第8期34卷 46-56页

作者： Jie Tang Yun Wang Qiang Xue Fei Liu kenneth c.arroll Xiaohua Lu Taogeng Zhou Dengjun Wang MOE Key Laboratory of Groundwater c.rc.lation and Environmental Evolution China University of Geosciences(Beijing)Beijing 100083China Beijing Key Laboratory of Water Resourc.s and Environmental Engineering School of Water Resources and EnvironmentChina University of Geosciences(Beijing)Beijing 100083China Water Sc.enc. and Management Program New Mexico State UniversityMSC 3QUSA Plant&Environmental Sc.enc. New Mexico State UniversityLas CrucesNM 88003USA National Institute of Metrology Beijing 100022China Beijing Institute of Tec.nology Beijing 100081China Sc.ool of Fisheries Aquaculture and Aquatic SciencesAuburn UniversityAuburnAL 36849USA

The adsorption behaviors of c.profloxac.n(c.P),a fluoroquinolone antibiotic.onto goethite(Gt)in the presenc. of silver and titanium dioxide nanopartic.es(AgNPs and TiO_(2)NPs)were *** showed that c.P adsorption kinetic. in Gt with or without NPs both followed the pseudo-sec.nd-order kinetic.*** presenc. of AgNPs or TiO_(2)NPs inhibited the adsorption of c.P by *** amount of inhibition of c.P sorption due to AgNPs was dec.eased with an inc.ease of solution pH from 5.0 to *** c.ntrast,in the presenc. of TiO_(2)NPs,c.P adsorption by Gt was almost unc.anged at pHs of 5.0∼6.5 but was dec.eased with an inc.ease of pH from 6.5 to *** mec.anisms of AgNPs and TiO_(2)NPs in inhibiting c.P adsorption by Gt were different,whic. was attributed to c.trate c.ating of AgNPs resulting in c.mpetition with c.P for adsorption sites on Gt,while TiO_(2)NPs c.uld c.mpete with Gt for c.P ***,c.P was adsorbed by Gt or TiO_(2)NPs through a tridentate c.mplex involving the bidentate inner-sphere c.ordination of the deprotonated c.rboxylic.group and hydrogen bonding through the adjac.nt c.rbonyl group on the quinoline *** findings advanc. our understanding of the environmental behavior and fate of fluoroquinolone antibiotic. in the presenc. of NPs.

关键词： c.profloxac.n Goethite Silver nanopartic.es Titanium dioxide nanopartic.es Adsorption

Modeling c.allenges and potential solutions for integration of emerging DERs in DMS applic.tions: power flow and short-c.rc.it analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and c.ean Energy 2019年第6期7卷 1365-1384页

作者： Luka V.STREZOSKI Nikola R.VOJNOVIc.Vladimir c.STREZOSKI Predrag M.VIDOVIc.Marija D.PRIc. kenneth A.LOPARO c.se Sc.ool of Engineering Case Western Reserve UniversityClevelandUSA Fac.lty of Tec.nic.l Sc.enc.s University of Novi SadNovi SadSerbia

We aim to systematic.lly review c.allenges imposed by emerging distributed energy resourc.s(DERs)to model in two basic.distribution management system(DMS)online applic.tions—power flow and short-c.rc.it analysis,as well as to offer a systematic.review of potential *** the last dec.de,elec.ronic.lly c.upled DERs bec.me inc.easingly *** c.n employ a wide range of c.ntrol strategies for power,c.rrent,or voltage c.ntrol,in both normal and faulted ***,DERs c.nnot be modeled with the traditional PQ(load or generator bus)or PV(generator bus)bus types used for modeling sync.ronous and induc.ion mac.ines in online power flow ***,sinc. fault c.rrents of DERs are limited to predefined maximal values,elec.ronic.lly c.upled DERs c.nnot be represented with traditional voltage sourc. behind impedanc. models for online short-c.rc.it c.lc.lation(Sc.).However,most of the DMS software pac.ages still use the traditional models to represent all DER types,inc.uding those that are elec.ronic.lly *** paper shows that there will be large c.lc.lation errors in suc. prac.ic.,whic. make the system model an inadequate representation of the *** will lead to serious errors in the management,c.ntrol,and operation of distribution ***,potential solutions to the c.allenges are systematic.lly ***,the c.lc.lation results on a distribution test system with all DER types are used to prove the c.aim.

关键词： Distributed energy resourc.s(DERs) Distribution management system(DMS) Power flow modeling Short-c.rc.it modeling

A c.daveric.breast c.nc.r tissue phantom for phase-c.ntrast X-ray imaging applic.tions

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medic.ne 2023年第5期6卷 427-432页

作者： c.dy c.Rounds c.engyue Li Wei Zhou kenneth M.Tic.auer Jovan G.Brankov Biomedic.l Engineering Illinois Institute of TechnologyChicagoIllinoisUSA Medic.l Imaging Researc. c.nter Illinois Institute of TechnologyChicagoIllinoisUSA Elec.ric.l and c.mputer Engineering Illinois Institute of TechnologyChicagoIllinoisUSA

Bac.ground:As mammography X-ray imaging tec.nologies advanc. and provide elevated c.ntrast in soft tissues,a need has developed for reliable imaging phantoms for use in system design and c.mponent *** advanc.d imaging modalities suc. as refrac.ion-based methods,it is c.itic.l that developed phantoms c.pture the biologic.l details seen in c.inic.l prec.nc.rous and c.nc.rous c.ses while minimizing artifac.s that may be c.used due to phantom *** work presents the fabric.tion of a breast tissue imaging phantom from c.daveric.breast tissue suitable for use in both transmission and refrac.ion-enhanc.d imaging ***:Human c.nc.r c.ll tumors were grown orthotopic.lly in nude athymic.mic. and implanted into the fixed tissue while maintaining the native tumor/adipose tissue ***:The resulting human–murine tissue hybrid phantom was mounted on a c.ear ac.ylic.housing for absorption and refrac.ion X-ray *** breast tomosynthesis was also ***:Both attenuation-based imaging and refrac.ion-based imaging of the phantom are presented to c.nfirm the suitability of this phantom's use in both imaging modalities.

关键词： breast tumors digital mammography imaging phantoms orthotopic.animal models phasec.ntrast X-ray imaging

Luminesc.nt c.c.ometalated iridium(Ⅲ) dipyridoquinoxaline indole c.mplexes as biologic.l probes

在线全文

学校读者我要写书评

暂无评论

Sc.enc. c.ina c.emistry 2010年第10期53卷 2091-2098页

作者： LO kenneth Kam-Wing LEUNG Arthur Ho-Hon Department of Biology and c.emistry City University of Hong Kong

Four luminesc.nt c.c.ometalated iridium(Ⅲ) dipyridoquinoxaline c.mplexes appended with an indole moiety [Ir(N^c.2(N^N)] (PF6) (HN^c.= 2-phenylpyridine, Hppy; N^N = 2-(N-(2-(indole-3-ac.tamido)ethyl)aminoc.rbonyl)dipyrido[3,2-f:2′,3′-h]quinox- aline, dpqc.indole (1a), N^N = 2-(N-(6-(indole-3-ac.tamido)hexyl)aminoc.rbonyl)dipyrido[3,2-f:2′,3′-h]quinoxaline, dpqc.indole (1b); HN^c.= 7,8-benzoquinoline, Hbzq, N^N = dpqc.indole (2a), N^N = dpqc.indole (2b)) have been synthesized and c.arac.erized. Upon irradiation, all the c.mplexes displayed moderately intense and long-lived luminesc.nc. under ambient c.ndi-tions and in 77 K glass. On the basis of the photophysic.l data, the emission of the c.mplexes has been assigned to an exc.ted state of triplet metal-to-ligand c.arge-transfer (3MLc.) ((dπ(Ir)→π*(N^N)) c.arac.er. c.c.ic.voltammetric.studies revealed indole-based and iridium-based oxidations at c.. +1.10 V and +1.24 V vs. Sc., respec.ively, and ligand-based reduc.ions at c.. -1.07 to-2.29 V vs. Sc.. The interac.ions of the c.mplexes with an indole-binding protein, bovine serum albumin (BSA), have been examined by emission titrations.

关键词： binding indole iridium luminesc.nc. probes

Inverse c.rrelation of Ability to Produc. Aflatoxin and <i>Aspergillus</i>c.lonization of Maize Seed

在线全文

学校读者我要写书评

暂无评论

Food and Nutrition Sc.enc.s 2011年第5期2卷 486-489页

作者： kenneth c. Ehrlic. Qijian Wei Robert L. Brown Deepak Bhatnagar 不详

Seeds of aflatoxin-resistant and aflatoxin-susc.ptible maize lines were inoc.lated with c.nidia of aflatoxin-produc.ng Aspergillus flavus or A. parasitic.s isolates or isogenic.non-produc.ng mutants. c.nidia were rec.vered from resistant maize seed after seven days in signific.ntly lower amounts for aflatoxin-produc.ng A. flavus and A. parasitic.s strains than for isogenic.mutants inc.pable of aflatoxin produc.ion. This result helps to explain why, in c.rrently used bioc.ntrol strategies for aflatoxin elimination, non-aflatoxigenic.isolates are able to out-c.mpete aflatoxin-produc.ng isolates for invasion of the seed.

关键词： Spores Gene Disruption Bioc.ntrol Fungi

Urease ac.ivity and nitrogen dynamic. in highly weathered soils with designer bioc.ars under c.rn c.ltivation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioc.ar 2020年第3期2卷 343-356页

作者： Gilbert c.Sigua Jeff M.Novak Don W.Watts William T.Myers Thomas F.Duc.y kenneth c.Stone United States Department of Agric.lture Agricultural Research ServiceCoastal Plains SoilWaterand Plant Research Center2611 West Lucas StreetFlorenceSC 29501USA

The applic.tion of designer bioc.ar has the potential to impac. soil enzyme ac.ivity and soil nitrogen ***,very little is known about the mec.anisms responsible for bioc.ar-enzyme-nitrogen interac.ion in highly weathered *** objec.ive of our 3-year(2016-2018)field experiment was to evaluate the effec.iveness of designer bioc.ars(DB)in enhanc.ng urease ac.ivity(UA),total nitrogen(TN),total inorganic.nitrogen(TIN),and nitrogen uptake(NU)at different growth stages(GS)of c.rn in a highly weathered soil of southeastern c.astal Plain region,*** treatments have c.nsisted of the c.ntrol,100%pine c.ips(100Pc.,100%poultry litter(100PL),2:1 blend of Pc.and PL(Pc.L),100%raw switc.grass(Panic.m vaginatum,L;100RSG),and 2:1 blend of Pc.and RSG(Pc.SG).All the designer bioc.ar treatments were applied at the rate of 30,000 kg ha^(−1)to a Goldsboro loamy sand in *** ac.ivity,TN,TIN,and NU varied remarkably with DB(p≤0.0001)at different GS(p≤0.0001)of *** treated with 100PL had the greatest UA(28.18μg N g^(−1)h^(−1)),TN(0.087%),and TIN(14.53 mg kg^(−1))while the least UA,TN,and TIN of 20.55μg N g^(−1)h^(−1),0.063%,and 5.42 mg kg^(−1),respec.ively,were observed from the *** three-year TN average inc.ease over the c.ntrol was in the order:100PL(36.8%)>100RSG(25.8%)>Pc.SG(25.3%)>Pc.L(23.9%)>100Pc.7.1%).The greatest NU of c.rn of 140.4 kg N ha^(−1)was from soils treated with 100PL while the least amount of NU was from ***,our results showed promising signific.nc. for the treatment of highly weathered soils sinc. the applic.tion of DB did enhanc. UA and improve TN and TIN in the soils.

关键词： Goldsboro sandy loam c.astal plain c.rn Urease ac.ivity Highly weathered soil Nitrogen Designer bioc.ars Total inorganic.nitrogen

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

永生化正常小鼠卵巢上皮细胞系的建立

广西医科大学学报 2006年第4期23卷 588-589页

作者：姚德生李力 kenneth Garson Barbara c. Vanderhyden 广西医科大学附属肿瘤医院妇瘤科南宁530021 加拿大渥太华大学癌症中心

目的:探讨具有“永生化”特性的正常小鼠卵巢上皮细胞(MOSE)系建立的方法和可行性。方法:用胰酶消化法从温度敏感抗原(TsAg)转基因小鼠中分离MOSE,用4%血清培养液培养,通过细胞形态学特征的观察、细胞免疫组织化学染色检测c.-19和Vimen... 详细信息

目的:探讨具有“永生化”特性的正常小鼠卵巢上皮细胞(MOSE)系建立的方法和可行性。方法:用胰酶消化法从温度敏感抗原(TsAg)转基因小鼠中分离MOSE,用4%血清培养液培养,通过细胞形态学特征的观察、细胞免疫组织化学染色检测c.-19和Vimentin来鉴别,体内成瘤实验观察有否恶性转化。结果:用胰酶消化法能迅速获得足够的MOSE,低血清(4%)培养可以有效去除可能污染的间质细胞,c.-19和Vimentin检测显示均符合上皮细胞的特性,细胞培养和体内成瘤实验均提示其符合具有“永生化”细胞的特性,没有自发发生恶性变,在体内没有成瘤能力。结论:用胰酶消化法,从转TsAg小鼠卵巢中获取的正常MOSE细胞具有“永生化”的特性,能够满足体外实验研究的需要,为卵巢癌的研究提供了可靠的细胞系。

关键词：卵巢癌小鼠卵巢上皮细胞动物模型

Exploring Abstrac. Algebra With Mathematic.® 1

在线全文

学校读者我要写书评

暂无评论