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Assessment of d ifferent f ormalin c oncentrations on the h istochemical s taining of l iver,k idneys and l ungs of Wistar r ats

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Toxicology Advances 2023年第3期5卷 22-27页

作者： Gideon Taiwo Oluwaloye Ochuko Fortunate Jenakevwe kenneth chukwudi onyegbula Julius Kayode Bankole Seyi Samson Enitan Department of Medical Laboratory Science Babcock UniversityIlisan-Remo121103Ogun StateNigeria Department of Biomedical Laboratory Science University of IbadanIbadan200005Oyo StateNigeria Department of Medical Laboratory Science Ambrose Alli UniversityEkpoma310006Edo StateNigeria

Background:Formaldehyde is a chemical substance used to preserve the biochemical and physiological components of a tissue by preventing autolysis and putrefaction,leaving the tissue in a life-like ***:This study aims to assess the effect of formalin concentrations on the histochemical staining in liver,kidneys and lungs of Wistar ***:A total of ten(10)Wistar rats were purchased and allowed to acclimatize for a period of 2 weeks in plastic cages with iron netting at the Animal House facility,Babcock *** animals were placed on standard rat feeds and water *** rats were randomly sacrificed by cervical dislocation,following mild anaesthesia using chloroform *** histological examination,the liver,lungs,and kidneys of Wistar rats were removed and preserved in neutral buffered formalin at 2%,4%,6%,8%,and 10%*** were stained in Haematoxylin and Eosin(Vector Laboratories,***,US).The quality of the nuclei,cytoplasm and cell membrane were graded on a scale of 1(poor)to 5(excellent).Results:Six percent(6%)formalin was observed to produce excellent results in liver tissues with a total grade of sixteen(16).10%formalin produced excellent results in lung tissues with a total grade or nineteen(19).Interestingly,2%,6%and 10%formalin concentrations produced excellently results in kidney tissues with the highest total grade fourteen(14).Conclusion:This study concludes that 6%formalin produced better section and staining qualities more than the commonly used 10%formalin according to the observations from the stained *** implies that the concentration of formalin affects the histochemical staining in liver,kidneys and lungs of Wistar rats.

关键词： fixation kidney liver lungs neutral buffered formalin Wistar rats

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Comparison of the Serum Proteins and Immune Responses of Velogenic Newcastle Disease Virus Infected Chickens and Ducks

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Open Journal of Veterinary Medicine 2014年第6期4卷 122-128页

作者： Chekwube Paul Eze Vincent S. O. Shoyinka John Osita Arinze Okoye Wilfred Sunday Ezema Innocent Okonkwo Ogbonna Didacus Chukwuemeka Eze Emmanuel chukwudi Okwor Ogbu kenneth Ikejiofor Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka Nigeria Microbiology Unit Department of Biological Sciences University of Agriculture Makurdi Nigeria National Veterinary Research Institute Vom Nigeria

The effect of velogenic Newcastle disease virus (vNDV) on the immune responses and serum proteins was investigated in six-week-old ducks and chickens. Results showed that weight loss was markedly significant (p < 0.05) from days 3 - 21 (PI) in chickens and mild (p < 0.05) on days 3 and 15 PI in ducks. The antibody response obtained showed significant (p < 0.05) increase in infected chickens (IC) than those of the infected ducks (ID). While the total serum protein and serum globulin increased significantly (p < 0.05) in IC on days 7 and 14 PI, they decreased significantly (p < 0.05) in ID only on day 21 PI. The immune responses and serum protein values in this experiment X-ray showed less susceptibility of ducks when compared with the chickens. This may be related to marked anorexia and severe dehydration observed in the latter consequent upon serum concentration. Ducks could be maintaining the endemicity of Newcastle disease (ND) as reservoir host.

关键词： Velogenic Newcastle Disease Experimental Infection Immune Response Serum Proteins Ducks Chickens

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