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Intraprocedural gastric juice analysis as compared to rapid urease test for real-time detection of Helicobacter pylori

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World Journal of Gastroenterology 2023年第10期29卷 1638-1647页

作者： Riccardo Vasapolli florent Ailloud Sebastian Suerbaum Jens Neumann Nadine Koch Lukas Macke Jörg Schirra Julia Mayerle Peter Malfertheiner Christian schulz Medical DepartmentⅡ University Hospital LMU MunichMunich 81377Germany Deutsches Zentrum für Infektionsforschung Partner Site MunichMunich 81377Germany Max von Pettenkofer Institute Faculty of MedicineLudwig-Maximilians University of MunichMunich 80336Germany National Reference Center for Helicobacter pylori Munich 81377Germany Institute of Pathology Faculty of MedicineLudwig-Maximilians-University of MunichMunich 81377Germany

BACKGROUND Endofaster is an innovative technology that can be combined with upper gastrointestinal endoscopy(UGE)to perform gastric juice analysis and real-time detection of Helicobacter pylori(***).AIM To assess the diagnostic performance of this technology and its impact on the management of *** in the real-life clinical *** Patients undergoing routine UGE were prospectively *** were taken to assess gastric histology according to the updated Sydney system and for rapid urease test(RUT).Gastric juice sampling and analysis was performed using the Endofaster,and the diagnosis of *** was based on real-time ammonium *** detection of *** served as the diagnostic gold standard for comparing Endofaster-based *** diagnosis with RUT-based *** *** A total of 198 patients were prospectively enrolled in an *** diagnostic study by Endofasterbased gastric juice analysis(EGJA)during the *** for RUT and histological assessment were performed on 161 patients(82 men and 79 women,mean age 54.8±19.2 years).*** infection was detected by histology in 47(29.2%)***,the sensitivity,specificity,accuracy,positive predictive value,and negative predictive value(NPV)for *** diagnosis by EGJA were 91.5%,93.0%,92.6%,84.3%,and 96.4%,*** patients on treatment with proton pump inhibitors,diagnostic sensitivity was reduced by 27.3%,while specificity and NPV were *** and RUT were comparable in diagnostic performance and highly concordant in *** detection(κ-value=0.85).CONCLUSION Endofaster allows for rapid and highly accurate detection of *** during *** may guide taking additional biopsies for antibiotic susceptibility testing during the same procedure and then selecting an individually tailored eradication regimen.

关键词： Helicobacter pylori diagnostic Chronic gastritis Gastric juice Endofaster Rapid urease test Antimicrobial susceptibility testing

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A sandwich-like structure hybrid coating of cold galvanizing coating/polydopamine on hot-dip galvanized steel with enhanced adhesion and corrosion resistance

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Science China(Technological Sciences) 2023年第8期66卷 2381-2395页

作者： LI JiWen GAO NingJie ZHANG WanYu XU Long NWOKOLO kenneth Izuchukwu WU Hao LIU fuChun HAN En-Hou Key Laboratory of Nuclear Materials and Safety Assessment Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China Institute of Corrosion Science and Technology Guangzhou 510530China Jihua Laboratory Foshan Guangdong ProvinceFoshan 528000China

This study aims to enhance the adhesion strength and anti-corrosion performance of the cold galvanizing coating(CGC)applied on the hot-dip galvanized steel(HDG).Polydopamine(PDA)is deposited on the HDG surface with different time ranges and as an interlayer between CGC and HDG through covalent *** surface morphology and the covalent interaction between PDA/HDG are exhibited by scanning electron microscope(SEM),atomic force microscopy(AfM)and X-ray photoelectron spectroscopy(XPS).The pull-off adhesion tests before and after neutral slat spry tests show an enhanced dry adhesion strength and less adhesion loss of the hybrid CGC/PDA coated HDG compared with the direct CGC coated *** addition,open circuit potential(OCP)reveals that the corrosion protection performance of the hybrid CGC/PDA coated HDG increases by 200%(up to 201 d)and the corrosion density icorrattaining about 4.45×10^(-7)A/cm^(2).Electrochemical impedance spectroscopy(EIS)measurements and X-ray diffraction(XRD)analysis confirm that the precipitate of the stable chelation formed by PDA and Zn^(2+)between CGC and HDG substrate can also improve the corrosion protection *** a strategy of strengthening adhesion and forming the chelate compound at the HDG surface promises a new route to corrosion protection of CGC on HDG.

关键词： dopamine zinc-rich coatings hot-dip galvanized steel corrosion protection chelate compound

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fluorescence Intensity Decay Shape Analysis Microscopy （fIDSAM） for Quantitative and Sensitive Live-Cell Imaging： A Novel Technique for fluorescence Microscopy of Endogenously Expressed fusion-Proteins

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Molecular Plant 2010年第3期3卷 555-562页

作者： frank Schleifenbaum Kirstin Elgass Marcus Sackrow Katharina Caesar kenneth Berendzen Alfred J. Meixner Klaus Hatter Center for Plant Molecular Biology Department of Plant Physiology University of Tubingen Auf der Morgenstelle 1 72076 Tubingen Germany Institute of Physical and Theoretical Chemistry University of Tubingen Auf der Morgenstelle 8 72076 Tubingen Germany

fluorescent studies of living plant cells such as confocal microscopy and fluorescence lifetime imaging often suffer from a strong autofluorescent background contribution that significantly reduces the dynamic image contrast and the quantitative access to sub-cellular processes at high spatial resolution. Here, we present a novel technique--fluorescence intensity decay shape analysis microscopy （fIDSAM） to enhance the dynamic contrast of a fluorescence image of at least one order of magnitude. The method is based on the analysis of the shape of the fluorescence intensity decay （fluorescence lifetime curve） and benefits from the fact that the decay patterns of typical fluorescence label dyes strongly differ from emission decay curves of autofluorescent sample areas. Using fIDSAM, we investigated Arabidopsis thaliana hypocotyl cells in their tissue environment, which accumulate an eGfP fusion of the plasma membrane marker protein LTI6b （LTI6b-eGfP） to low level. Whereas in conventional confocal fluorescence images, the membranes of neighboring cells can hardly be optically resolved due to the strong autofluorescence of the cell wall, fIDSAM allows for imaging of single, isolated membranes at high spatial resolution. Thus, fIDSAM will enable the sub-cellular analysis of even low-expressed fluorophoretagged proteins in living plant cells. furthermore, the combination of fIDSAM with fluorescence lifetime imaging provides the basis to study the local physico-chemical environment of fluorophore-tagged biomolecules in living plant cells.

关键词： Cell structure cell walls membrane proteins high-resolution fluorescence microscopy.

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Phylogenetic Relationships of <i>Termitomyces aurantiacus</i>Inferred from Internal Transcribed Spacers DNA Sequences

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Advances in Bioscience and Biotechnology 2015年第5期6卷 358-367页

作者： Shafiquzzaman Siddiquee Kobun Rovina Laila Naher kenneth f. Rodrigues Md Akhter Uzzaman Biotechnology Research Institute Universiti Malaysia Sabah Kota Kinabalu Malaysia faculty of Agro-Based Industry Universiti Malaysia Kelantan Jeli Campus-17600 Kelantan Malaysia Planning and Development Division Bangladesh Atomic Energy Commission Dhaka Bangladesh

fungus-growing termites cultivate species of the mutualistic basidiomycete genus Termitomyces on a substrate called the fungal comb. Identification of fungal species based on morphological features is complicated, tedious, and prone to errors. As an alternative, nuclear ribosomal DNA sequences consisting of the internal transcribed spacers (ITS1 and ITS2) and 5.8S rDNA were used to identify Malaysian isolates of Termitomyces sp. The morphological characteristics and molecular data indicate that Malaysian Termitomyces isolated is clearly monophyletic and belongs to the Tricholomataceae family. The Malaysian isolates analyzed in this study represent the termite fungus species called T. aurantiacus.

关键词： fungal Comb Internal Transcribed Spacers Morphological feature Phylogenetic Relationship

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Magnetic resonance imaging of the liver:New imaging strategies for evaluating focal liver lesions

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World Journal of Radiology 2009年第1期1卷 72-85页

作者： kenneth Coenegrachts Department of Radiology AZ St.-Jan Brugge-Oostende AVRuddershove 10B-8000 BrugesBelgium Author contributions:Coenegrachts K contributed solely to this paper

The early detection of focal liver lesions,particularly those which are malignant,is of utmost *** resection of liver metastases of some malignancies(including colorectal cancer)has been shown to improve the survival of *** knowledge of the number,size,and regional distribution of liver metastases is essential to determine their *** all focal liver lesions larger than 10 mm are demonstrated with current imaging techniques but the detection of smaller focal liver lesions is still relatively *** of the advantages of magnetic resonance imaging(MRI)of the liver is better soft tissue contrast(compared to other radiologic modalities),which allows better detection and characterization of the focal liver lesions in *** in MRI hardware and software and the availability of novel MRI contrast agents have further improved the diagnostic yield of MRI in lesion detection and *** the primary modalities for liver imaging are ultrasound and computed tomography,recent studies have suggested that MRI is the most sensitive method for detecting small liver metastatic lesions,and MRI is now considered the pre-operative standard method for *** recent developments in MRI sequences for the upper abdomen comprise unenhanced diffusion-weighted imaging(DWI),and keyhole-based dynamic contrast-enhanced(DCE)MRI(4D THRIVE).DWI allows improved detection(b=10 s/mm2)of small(focal liver lesions in particular,and is useful as a road map ***,using higher b-values,the calculation of the apparent diffusion coefficient value,true diffusion coefficient,D,and the perfusion fraction,f,has been used for the characterization of focal liver *** 4D THRIVE enables MRI of the liver with high temporal and spatial resolution and full liver coverage.4D THRIVE improves evaluation of focal liver lesions,providing multiple arterial and venous phases,and allows the calculation of perfusion parameters usi

关键词： Magnetic resonance imaging Liver neoplasms Diffusion-weighted magnetic resonance imaging 4D THRIVE Dynamic contrast-enhanced magnetic resonance imaging Contrast agents

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Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods

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Cellular & Molecular Immunology 2021年第4期18卷 936-944页

作者： Berislav Bosnjak Saskia Catherina Stein Stefanie Willenzon Anne Katrin Cordes Wolfram Puppe Gunter Bernhardt Inga Ravens Christiane Ritter Christian R.Schultze-florey Nina Godecke Jorg Martens Hannah Kleine-Weber Markus Hoffmann Anne Cossmann Mustafa Yilmaz Isabelle Pink Marius M.Hoeper Georg M.N.Behrens Stefan Pohlm ann Rainer Blasczyk Thomas f.schulz Reinhold forster Institute of Immunology Hannover Medical SchoolHannoverGermany lnstitute of Virology Hannover Medical SchoolHannoverGermany Department of Hematology HemostasisOncology and Stem Cell TransplantationHannover Medical SchoolHannoverGermany Institute of Transfusion Medicine and Transplant Engineering Hannover Medical SchoolHannoverGermany German Primate Center-Leibniz Institute for Primate Research GottingenGermany faculty of Biology and Psychology University GottingenGottingenGermany Department of Rheum atology and Immunology Hannover Medical SchoolHannoverGermany Public Health Department HannoverGermany Department of Pneumology and German Centre of Lung Research(DZL) Hannover Medical SchoolHannoverGermany German Center for Infection Research(DZIf) partner site Hannover-BraunschweigHannoverGermany Cluster of Excellence RESIST(EXC 2155) Hannover Medical SchoolHannoverGermany

Neutralizing antibodies targeting the receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)block severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)entry into cells via surface-expressed angiotensin-converting enzyme 2(ACE2).We used a surrogate virus neutralization test(sVNT)and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus(VSV)vector-based neutralization assay(pVNT)to assess the degree to which serum antibodies from coronavirus disease 2019(COVID-19)convalescent patients interfere with the binding of SARS-CoV-2 S to *** tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent ***,sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA ***,levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient *** to pVNT,sVNT is less sophisticated and does not require any biosafety *** this assay is also much faster and cheaper,sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy.

关键词： COVID-19 SARS-CoV-2 Neutralizing antibody Serum ELISA

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Weedy rice, a hidden gold mine in the paddy field

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Molecular Plant 2022年第4期15卷 566-568页

作者： Dongya Wu Jie Qiu Jian Sun Beng-Kah Song kenneth M.Olsen Longjiang fan Zhejiang University City College Hangzhou 310015China College of Agriculture and Biotechnology Zhejiang UniversityHangzhou 310058China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Rice Research Institute Shenyang Agricultural UniversityShenyang 110866China School of Science Monash University MalaysiaBandar SunwaySelangor 46150Malaysia Department of Biology Washington University in St.LouisSt.LouisMO 63130USA

Weedy rice(Oryza spp.)is an increasingly invasive paddy weed,which mimics cultivated rice at the seedling stage but is distinguished from cultivated rice with strong seed shattering and seed dormancy,which are typical phenotypic characteristics of wild *** the origins of this conspecific form of cultivated rice(Oryza sativa),four hypotheses have been proposed and debated for decades:(1)origination from wild rice(Oryza rufipogon or possibly other wild Oryza species);(2)origination following hybridization between wild and cultivated rice;(3)direct descent from escaped cultivated rice;and(4)intervarietal hybridization between different domesticated rice varieties(Ellstrand et al.,2010).Recent population genomic investigations based on whole-genome variation provide powerful phylogenetic and demographic evidence for the de-domestication origination of weedy rice(Li et al.,2017;Qiu et al.,2017,Qiu et al.,2020;Sun et al.,2019).

关键词： sativa (Oryza spp.)

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SPIRIT 2013声明：定义临床研究方案的标准条目

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中国循证医学杂志 2013年第12期13卷 1501-1507页

作者： An-Wen Chan Jennifer M.Tetzlaff Douglas G.Altman Andreas Laupacis Peter C.GΦtzsche Karmela Krleza-Jeri AsbjΦrn Hróbjartsson Howard Mann Kay Dickersin Jesse A.Berlin Caroline J.Doré Wendy R.Parulekar William S.M.Summerskill Trish Groves kenneth f.schulz Harold C.Sox frank W.Rockhold Drummond Rennie David Moher 钟丽丹郑颂华吴泰相李幼平商洪才张伯礼唐旭东吕爱平卞兆祥 SPIRIT中文工作組

临床研究方案是临床研究设计、实行、报告和评价的基础。然而，临床研究方案及现存的临床研究方案指引在内容和质量上差异很大。本文描述了《规范临床研究方案内容》（Standard Protocol Items：Recommendations for Interventional Tri... 详细信息

临床研究方案是临床研究设计、实行、报告和评价的基础。然而，临床研究方案及现存的临床研究方案指引在内容和质量上差异很大。本文描述了《规范临床研究方案内容》（Standard Protocol Items：Recommendations for Interventional Trials 2013 ）,简称SPIRIT2013声明，一个临床研究方案必须报告的条目指引的系统建立和范围。

关键词：临床研究条目标准

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Appearance of aseptic vascular grafts after endovascular aortic repair on[(18)f]fluorodeoxyglucose positron emission tomography/computed tomography

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World Journal of Radiology 2023年第8期15卷 241-249页

作者： Paige Bennett Maria Bernadette Tomas Christopher f Koch kenneth J Nichols Christopher J Palestro Department of Radiology LIJMC Northwell HealthNew Hyde ParkNY 11040United States Department of Radiology Zucker School of Medicine at Hofstra/NorthwellHempsteadNY 11549United States Division of Nuclear Medicine and Molecular Imaging Department of RadiologyNorthwell HealthNew Hyde ParkNY 11040United States

BACKGROUND Diagnosis of prosthetic vascular graft infection with[(18)f]fluorodeoxyglucose positron emission tomography/computed tomography(18f-fDG PET/CT)allows for early detection of functional changes associated with infection,based on increased glucose utilization by activated macrophages and *** vascular grafts,like all foreign bodies,can stimulate an inflammatory response,which can present as increased activity on 18f-fDG PET/***,distinguishing aseptic inflammation from graft infection,though important,can be *** the case of endovascular aneurysm repair(EVAR),a minimally invasive procedure involving the transfemoral insertion of an endoprosthetic stent graft,the normal postoperative appearance of these grafts on 18f-fDG PET/CT can vary over time,potentially confounding study *** To investigate the visual,semiquantitative,and temporal characteristics of aseptic vascular grafts in patients status post *** In this observational retrospective cohort study,patients with history of EVAR who underwent 18f-fDG PET/CT for indications other than infection were identified *** patients were asymptomatic for graft infection-no abdominal pain,fever of unknown origin,sepsis,or leukocytosis-at the time of imaging and for≥2 mo after each PET/*** studies such as CT for each patient were also reviewed,and any patients with suspected or confirmed vascular graft infection were *** hundred two scans performed on 43 patients(34 males;9 females;age=77±8 years at the time of the final PET/CT)were retrospectively *** 43 patients had an abdominal aortic(AA)vascular graft,40 patients had a right iliac(RI)limb graft,and 41 patients had a left iliac(LI)limb *** patients had 1 PET/CT and 21 patients had from 2 to 9 PET/*** were imaged between 2 mo to 168 mo(about 14 years)post *** grafts were imaged within 6 mo of placement,including three that were imaged wit

关键词： Aseptic vascular grafts Endovascular aortic repair [(18)f]fluorodeoxyglucose positron emission tomography/computed tomography

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Philinopsides A and B,Two New Sulfated Saponins from the Sea Cucumber Pentacta quadrangularis

Philinopsides A and B,Two New Sulfated Saponins from the Sea...

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中国海洋生化学术会议

作者： Chih-Chuang Liaw kenneth f.Bastow Kuo-Hsiung Lee Natural Products Laboratory School of Pharmacy University of North Carolina Chapel Hill NC 27599-7360 USA

Two new sulfated saponins, philinopsides A (1) and B (2), have been isolated from the sea cucumber Pentacta quadrangularis. Their structures were established by a combination of NMR (600 MHZ) spectroscopic analyses and chemical transformations. Based on DQCOSY, HMQC, NOESY, HMBC and ESIMS data, philinopsides A and B were shown to have the structures 16 β-acetoxy-3-O-{3’’’’ -O-methyl- β-D-glucopyranosyl-(1 →3)- β-D-xylopyranosyl-(1 →4)- β-D-quinovopyranosyl-(1→2)-4’ -O-sodium sulfate- β-D-xylopyranosyl}-holosta-7, 24- diene- 3 β-ol and 16β-acetoxy-3-O-{3’’’’-O-methyl-β-D-glucopyranosyl-(1→3)-2’’’-O -sodium sulfate-β-D-xylo- pyranosyl-(1→4)- β-D-quinovo-pyranosyl-(1→2)-4’-O-sodium sulfate- β-D-xylopyranosyl}-holosta- 7,25(26)-diene-3β-ol, respectively. Philinopside A (1) showed significant cytotoxic and anti-angiogenetic activities, although the former in vitro bioactivity was not markedly tumor-type selective.

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