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Molecular evidence and phylogenetic delineation of spotted fever group Rickettsia species in Amblyomma ticks from cattle in Gauteng and Limpopo Provinces,South Africa

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Asian Pacific Journal of Tropical Medicine 2023年第8期16卷 371-378页

作者： keneilwe mhlambo Benson C Iweriebor Nqobile Mkolo K O Afolabi Larry C Obi Department of Biology and Environmental Sciences School of Science and TechnologySefako Makgatho Health Sciences UniversityGa-RankuwaPretoriaSouth Africa Pathogenic Yeasts Research Group Department of Microbiology and BiochemistryUniversity of the Free StateBloemfontein 9300Free StateSouth Africa School of Science and Technology Sefako Makgatho Health Sciences UniversityGa-RankuwaPretoriaSouth Africa

Objective:To determine the prevalence of tick-borne pathogens with a particular focus on Rickettsia *** ticks collected from cattle in Gauteng and Limpopo Provinces,South ***:A total of 200 ticks were collected from cattle within the Madala livestock,Pretoria,Gauteng Province and in Mankweng Township,Polokwane,Limpopo Province in *** ticks were morphologically identified and processed individually for a total genomic DNA *** primers targetting ompA,ompB,and the 17KDa genes were used for a molecular screening and delineation of Rickettsia from the extracted genetic materials using polymerase chain reaction(PCR)*** amplicons of positive samples were sequenced bidirectionally using the Sanger sequencing *** generated were processed and analysed using appropriate bioinformatics ***:The ticks were morphologically identified as Amblyomma *** profiling of the genomic DNA samples revealed the presence of the Rickettsia pathogen in 42(21%)of the ticks collected from both *** of the genes profiled,14(7%)were positive for 17KDa,42(21%)for ompA and 32(16%)were positive for ompB genes *** nucleotide blast of the sequenced genomes showed high similarity,as high as 100% with other reference Rickettsia(R.)africae in the *** phylogenetic analysis of the sequences further validated them as *** with their characteristic clustering pattern with related reference ***:There is an abundance of *** in Amblyomma ticks collected from cattle in the study *** has serious public health implications as individuals who accidentally get infested with the ticks could acquire ***,adequate precautions in terms of sensitization of farmers about the risk and mass mobilization drive to control the vectors in the areas are highly recommended to safeguard public health.

关键词： Amblyomma ticks Tick-borne pathogen Rickettsia Cattle South Africa

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